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Gepard: a rapid and sensitive tool for creating dotplots on genome
Gepard: a rapid and sensitive tool for creating dotplots on genome

... quickly create complete comparisons of two proteins or nucleic acid sequences. Dotplots, which are the graphical results of dot matrix analysis, can be used to interpret and analyze the evolutionary relationships of the sequences by examining conserved domains, reverse matches and repeats. The princ ...
Artificial selection on introduced Asian haplotypes shaped the
Artificial selection on introduced Asian haplotypes shaped the

... reference genome in 2012 [19] greatly contributed to the applicability of these techniques in pig breeding. This genomic information can also be used to pinpoint regions in the genome that have been under selective pressure. The resulting changes at the DNA level have been detected as selective swee ...
Case-Parent Triads
Case-Parent Triads

... Any disease or condition that has its origins in fetal life would be eligible for study using the method described here. No rare-disease assumption is necessary. For this illustration, we assume the condition under study is a type of birth defect. We assume that an allele suspected of increasing the ...
NPTEL-Module-1: Introduction to Bioorganic Chemistry Dr. S. S. Bag
NPTEL-Module-1: Introduction to Bioorganic Chemistry Dr. S. S. Bag

... biradical specis via Bergman cyclization. In addition to that neocarzinostatin (NCS) chromophore which does not contain the classical conjugated enediyne system also demonstrated very similar DNA cleavage mechanism via the generation biradical species through the MyersSaito cyclization. The enediyne ...
Porphyrin Metabolism & Porphyrias
Porphyrin Metabolism & Porphyrias

... Cytochrome P450s (CYPs) are actually a superfamily of related, heme-containing monooxygenase enzymes that participate in abroad variety of reactions. This system performs different functions in two separate locations in cells. ...
Streptomyces roseoverticillatus produces two different poly(amino
Streptomyces roseoverticillatus produces two different poly(amino

... analysis of the silylated monomer determined that the repeating unit is glutamic acid (Fig. 2a). Enantioseparation using HPLC showed that almost all the glutamic acids are of the L-configuration (Fig. 2b): the L/D ratio was determined to be 98.3/1.7. A signal corresponding to Laspartic acid was also ...
The role of the PXY-CLE signalling pathway in regulating cell
The role of the PXY-CLE signalling pathway in regulating cell

... intellectual property (the “Intellectual Property”) and any reproductions of copyright works in the thesis, for example graphs and tables (“Reproductions”), which may be described in this thesis, may not be owned by the author and may be owned by third parties. Such Intellectual Property and Reprodu ...
Enzyme Mechanisms - Illinois Institute of Technology
Enzyme Mechanisms - Illinois Institute of Technology

... Prokaryotic and eukaryotic cells can, through mitosis, serve as factories to make many copies (> 106 in some cases) of a moderately complex segment of DNA—provided that that segment can be incorporated into a chromosome or a plasmid This is amplification ...
Contribution of defined amino acid residues to the immunogenicity
Contribution of defined amino acid residues to the immunogenicity

... STh have been mapped to a highly conserved domain including six cysteine residues forming three intramolecular disul¢de bonds that are absolutely necessary for toxicity of the molecule [5]. Because STa is non-immunogenic in its native form, several di¡erent approaches have been explored to obtain no ...
Strong seed pods - Bayer research Magazine
Strong seed pods - Bayer research Magazine

... compared with the original gene sequence, researchers could tell which canola genes had promising IND gene mutations. Using this method, the specialists at Bayer were able to reduce the huge pool of possibilities to some 20 candidates. Unwanted mutations also occurred outside of the IND gene, howeve ...
factor occupancy and gene expression Effects of
factor occupancy and gene expression Effects of

Molecular and General Genetics.
Molecular and General Genetics.

... growth primarily by colonising the root surface, causing an increase in the proliferation of the root hairs and root system of the host plant. This e€ect is thought to result from the production of auxin-like compounds, such as indole-3-acetic acid (IAA), by the bacterium, because application of IAA ...
Nitrogen Balance and Protein Requirements: Definition and
Nitrogen Balance and Protein Requirements: Definition and

... they must be introduced with the diet in a proportion that will fit with the organism’s metabolic needs. On the other hand, in the absence of dietary NEAA, despite the theoretical capability of the body to synthesise them, nitrogen will be needed for their de novo synthesis. This nitrogen in turn mu ...
File
File

... B) consists of a single rather than a double polynucleotide strand. C) contains the sugar ribose rather than the sugar deoxyribose. D) contains phosphate groups not found in RNA. Answer: A Topic: 3.15 Skill: Knowledge/Comprehension 61) You work for a company that manufactures food products. A new "w ...
factor occupancy and gene expression Effects of sequence variation
factor occupancy and gene expression Effects of sequence variation

... an allelic imbalance in occupancy. At many sites, TFs clustered in TF-binding hubs on the same homolog in especially open chromatin. While genetic variation in core TF binding motifs generally resulted in large allelic differences in TF occupancy, most allelic differences in occupancy were subtle an ...
Transgenic Glyphosate Resistance Crops
Transgenic Glyphosate Resistance Crops

Did sex chromosome turnover promote divergence of the major
Did sex chromosome turnover promote divergence of the major

... Because speciation was reckoned to require a long time during which changes were polymorphic, it was difficult to imagine that such drastic changes could remain in the population long enough to become fixed, and were, therefore, thought to follow, rather than to induce speciation. Yet drastic chromo ...
factor occupancy and gene expression Effects of sequence variation
factor occupancy and gene expression Effects of sequence variation

... an allelic imbalance in occupancy. At many sites, TFs clustered in TF-binding hubs on the same homolog in especially open chromatin. While genetic variation in core TF binding motifs generally resulted in large allelic differences in TF occupancy, most allelic differences in occupancy were subtle an ...
Electrochemical detection of polymerase reactions by specific metal
Electrochemical detection of polymerase reactions by specific metal

... the sequence of the template nucleic acid is already known the primer can be designed to be complementary to such a sequence. In one embodiment, the primer anneals to either the 3’ or the 5’ end of the template nucleic acid. The primer may be prepared using any suitable method, such as, for example, ...
3 -2 -1 -2 -1 1 2 K
3 -2 -1 -2 -1 1 2 K

... “Find-a-gene project” • The find-a-gene project is meant to be a very focused, specific project to help you understand how to use various BLAST tools (e.g. TBLASTN, BLASTX, BLASTP) and various databases. • You can start with (almost) any protein, from the organism of your choice, and discover a “no ...
Regulation of enzyme activity
Regulation of enzyme activity

Quantitative expression of cholera toxin mRNA in Vibrio cholerae
Quantitative expression of cholera toxin mRNA in Vibrio cholerae

... cholerae with El Tor strains with three different CTX arrangements, A, B and C. The results demonstrated the production of lower levels of CT mRNA in the El Tor strains with the various CTX arrangements. The Ct data also suggested that ctxAB gene expression was approximately fourfold and twofold hig ...
Gene Section FOXP3 (forkhead box P3) Atlas of Genetics and Cytogenetics
Gene Section FOXP3 (forkhead box P3) Atlas of Genetics and Cytogenetics

RNAi phenotypes are influenced by the genetic background of the
RNAi phenotypes are influenced by the genetic background of the

... Background: RNA interference (RNAi) is a powerful tool to study gene function in organisms that are not amenable to classical forward genetics. Hence, together with the ease of comprehensively identifying genes by new generation sequencing, RNAi is expanding the scope of animal species and questions ...
Exogenous glutamine increases lipid accumulation in
Exogenous glutamine increases lipid accumulation in

... (Qiagen, Valencia, CA) following the manufacturer’s protocol. cDNA libraries were constructed and then sequenced by Novogene (Beijing, China) using an Illumina HiSeq 2000 platform to generate single-end 50 bp reads. Sequencing raw data from each library was preprocessed using TopHat, allowing up to ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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