Structural Characterization of Humanized Nanobodies with
... an aerobic, non-spore-forming, Gram-negative coccobacillus, Bordetella pertussis [1]. In recent years, there has been an upsurge of whooping cough among elderly people [1] whose vaccination-induced protective immunity waned-off due to the lack of natural boosters caused by a decrease of circulating ...
... an aerobic, non-spore-forming, Gram-negative coccobacillus, Bordetella pertussis [1]. In recent years, there has been an upsurge of whooping cough among elderly people [1] whose vaccination-induced protective immunity waned-off due to the lack of natural boosters caused by a decrease of circulating ...
Molecular and Phylogenetic Characterization of Cytokine Genes
... Abstract: Bats (order Chiroptera) represent nearly one fourth of the approximately 5,000 known species of mammals, yet many aspects of their biology are poorly understood. Bats of certain species are well known reservoirs of rabies virus but within the past few years bats also have been identified a ...
... Abstract: Bats (order Chiroptera) represent nearly one fourth of the approximately 5,000 known species of mammals, yet many aspects of their biology are poorly understood. Bats of certain species are well known reservoirs of rabies virus but within the past few years bats also have been identified a ...
Intrinsically Disordered Domains of the B Cell Receptor
... Integral membrane proteins (MPs), embedded in the membrane, provide critical roles in cell-to-cell contact, cytoskeleton contact, surface recognition, signaling, enzymatic activity and transportation of substances across the membrane [1]. MPs are also important for drug research, accounting for over ...
... Integral membrane proteins (MPs), embedded in the membrane, provide critical roles in cell-to-cell contact, cytoskeleton contact, surface recognition, signaling, enzymatic activity and transportation of substances across the membrane [1]. MPs are also important for drug research, accounting for over ...
Chemically Mediated Site-Specific Proteolysis. Alteration of Protein
... of native proteins. One possible application of this new functionality includes the systematic removal of individual protein domains of putative higher order structures such that the functional contribution(s) of these structures can be dissected. Especially in complicated protein-protein complexes, ...
... of native proteins. One possible application of this new functionality includes the systematic removal of individual protein domains of putative higher order structures such that the functional contribution(s) of these structures can be dissected. Especially in complicated protein-protein complexes, ...
Phylogenetic relationship of phototrophic purple sulfur bacteria
... rRNA and pufLM genes. DNA extraction, primers, PCR, and sequencing. DNA was extracted by mechanical disruption of the cells using a bead mill (type MM200, Retsch, Germany). One ml of a freshly grown culture was centrifuged in a 1.5-ml tube (10 min, 8000 ×g). The supernatant was discarded and the cel ...
... rRNA and pufLM genes. DNA extraction, primers, PCR, and sequencing. DNA was extracted by mechanical disruption of the cells using a bead mill (type MM200, Retsch, Germany). One ml of a freshly grown culture was centrifuged in a 1.5-ml tube (10 min, 8000 ×g). The supernatant was discarded and the cel ...
Duchenne muscular dystrophy:
... protein synthesis estimates [ 10, 12]. Conversely, when plasma glutamine concentration is lowered following treatments with phenyl-butyrate, a glutamine chelator used in urea cycle defects, estimates of protein synthesis decrease significantly ...
... protein synthesis estimates [ 10, 12]. Conversely, when plasma glutamine concentration is lowered following treatments with phenyl-butyrate, a glutamine chelator used in urea cycle defects, estimates of protein synthesis decrease significantly ...
Protein Structure Prediction: On the cusp between Futility and
... • Low resolution scoring functions – knowledge based • from database of known protein structures • only meaningful when database is big • data mining? ...
... • Low resolution scoring functions – knowledge based • from database of known protein structures • only meaningful when database is big • data mining? ...
How flexible is α-actinin`s rod domain?
... greater than or equal to 150 pN generate an unfolded structure, and lower forces produce only slight extension. The protein as a whole also unfolds at ∼150 pN. The stretching of the helical linker, the flexible hexamer and the rigid hexamer are calculated as the whole protein is stretched (i.e. thei ...
... greater than or equal to 150 pN generate an unfolded structure, and lower forces produce only slight extension. The protein as a whole also unfolds at ∼150 pN. The stretching of the helical linker, the flexible hexamer and the rigid hexamer are calculated as the whole protein is stretched (i.e. thei ...
Learn more by reading original document
... weight matrices. The default for proteins in version 1.8 is the PAM series derived by Gonnet and colleagues. Note, a series is used! The actual matrix that is used depends on how similar the sequences to be aligned at this alignment step are. Different matrices work differently at each evolutionary ...
... weight matrices. The default for proteins in version 1.8 is the PAM series derived by Gonnet and colleagues. Note, a series is used! The actual matrix that is used depends on how similar the sequences to be aligned at this alignment step are. Different matrices work differently at each evolutionary ...
Compilation of tRNA sequences and sequences of
... (iii) Compilation of tRNA Genes, is a summary of the sequences of tRNA genes published in the literature and databases up to the end of 1998. It contains tRNA genes of all organisms and organelles, but is not updated since January 1999. This table contains about 350 sequences of cytoplasmic tRNA gen ...
... (iii) Compilation of tRNA Genes, is a summary of the sequences of tRNA genes published in the literature and databases up to the end of 1998. It contains tRNA genes of all organisms and organelles, but is not updated since January 1999. This table contains about 350 sequences of cytoplasmic tRNA gen ...
TAR-RNA binding by HIV-1 Tat protein is
... binding experiments demonstrate that the affinity of the mirrorimage (L-enantiomer) of wild-type TAR for the Tat protein is 1000-fold better, thereby matching the affinity of the D-sequence. Moreover, our data show that in both cases the stability of the complexes is significantly lowered upon chang ...
... binding experiments demonstrate that the affinity of the mirrorimage (L-enantiomer) of wild-type TAR for the Tat protein is 1000-fold better, thereby matching the affinity of the D-sequence. Moreover, our data show that in both cases the stability of the complexes is significantly lowered upon chang ...
UNIT 2. Structure and function of proteins.
... 2.3. Three-Dimensional structure and function of proteins. PROTEIN STRUCTURE: LEVELS OF ORGANIZATION: ...
... 2.3. Three-Dimensional structure and function of proteins. PROTEIN STRUCTURE: LEVELS OF ORGANIZATION: ...
Complete DNA sequence of yeast chromosome II.
... have been studied in great detail biochemically. Based on present data, one can calculate that a repertoire of 65007000 genes is sufficient to build this simple eukaryotic cell. Considering recent progress and worldwide studies of yeast genome sequencing (Vassarotti and Goffeau, 1992; Goffeau, 1994 ...
... have been studied in great detail biochemically. Based on present data, one can calculate that a repertoire of 65007000 genes is sufficient to build this simple eukaryotic cell. Considering recent progress and worldwide studies of yeast genome sequencing (Vassarotti and Goffeau, 1992; Goffeau, 1994 ...
Types and effects of protein variations. Vihinen
... different. In addition to functions, protein variants can change structures, properties, interactions and other characteristics in several ways. List of the effects would be really long and include e.g. interactions, stability, electrostatic effects, protein packing, local and global structural chan ...
... different. In addition to functions, protein variants can change structures, properties, interactions and other characteristics in several ways. List of the effects would be really long and include e.g. interactions, stability, electrostatic effects, protein packing, local and global structural chan ...
Evolution of the enzymes of the citric acid cycle and the
... the JTT-F martix and starting from the NJ tree of ML distances. We often encountered distantly related genes encoding related protein families for different enzyme activities. These were usually included in the analysis if they helped to elucidate a general evolution pattern within a gene family, bu ...
... the JTT-F martix and starting from the NJ tree of ML distances. We often encountered distantly related genes encoding related protein families for different enzyme activities. These were usually included in the analysis if they helped to elucidate a general evolution pattern within a gene family, bu ...
subset seed extension to protein blast
... defined through a cumulative contribution of amino acid matches, not necessarily identical. A short sequence of such matches is considered a hot spot if their additive contribution (score) exceeds a predefined threshold. It is thus not clear whether seedbased approaches may measure up with the cumul ...
... defined through a cumulative contribution of amino acid matches, not necessarily identical. A short sequence of such matches is considered a hot spot if their additive contribution (score) exceeds a predefined threshold. It is thus not clear whether seedbased approaches may measure up with the cumul ...
Are You suprised ?
... among organisms. Proteins consist of chains of amino acids. The sequence, or order, of the amino acids in a protein determines the type and nature of the protein. In turn, the sequence of amino acids in a protein is determined by the sequence of nucleotides in a gene. A change in the DNA nucleotide ...
... among organisms. Proteins consist of chains of amino acids. The sequence, or order, of the amino acids in a protein determines the type and nature of the protein. In turn, the sequence of amino acids in a protein is determined by the sequence of nucleotides in a gene. A change in the DNA nucleotide ...
chemical modification of carboxylic groups
... also by Mr values very close to 30000 (based on their known sequences), were run on SDS/PAGE and appeared to behave 'normally' (Table 1). Thus the anomalous behaviour of the Actinomadura R39 ,J-lactamase did not appear to be a common feature of the 30000-Mr class A /J-lactamases. Non-denaturing PAGE ...
... also by Mr values very close to 30000 (based on their known sequences), were run on SDS/PAGE and appeared to behave 'normally' (Table 1). Thus the anomalous behaviour of the Actinomadura R39 ,J-lactamase did not appear to be a common feature of the 30000-Mr class A /J-lactamases. Non-denaturing PAGE ...
Sequence - BIOTEC - Biotechnology Center TU Dresden
... n Microarrays n Measure activity of thousands of genes at the same time n Example: n Cancer n Compare activity with and without drug treatment n Result: Hundreds of candidate drug targets n RNAi (Noble prize 2004, Fire and Mello) n Knock-down genes and observe effect n Example: n Infectious diseases ...
... n Microarrays n Measure activity of thousands of genes at the same time n Example: n Cancer n Compare activity with and without drug treatment n Result: Hundreds of candidate drug targets n RNAi (Noble prize 2004, Fire and Mello) n Knock-down genes and observe effect n Example: n Infectious diseases ...
Stockholm University
... translational efficiency [44]. Previous studies have also shown that rare codon regions are more common in long proteins, as maybe expected by chance alone [25]. However, our analysis of the E. coli proteome indicated that rare codon regions occurring near the 5’ end of mRNAs are present in long and ...
... translational efficiency [44]. Previous studies have also shown that rare codon regions are more common in long proteins, as maybe expected by chance alone [25]. However, our analysis of the E. coli proteome indicated that rare codon regions occurring near the 5’ end of mRNAs are present in long and ...
Localization of different microsatellites and a minisatellite
... Identification of polytene chromosomes from the embryo suspensor of Phaseolus is hindered by great variation in chromosome morphology and because they are lacking a clear banding pattern. To overcome these problems, each chromosome should be tagged individually with molecular markers which can be de ...
... Identification of polytene chromosomes from the embryo suspensor of Phaseolus is hindered by great variation in chromosome morphology and because they are lacking a clear banding pattern. To overcome these problems, each chromosome should be tagged individually with molecular markers which can be de ...
Pavel Doležal
... inhabit oxygen-poor environments, do not possess mitochondria as we know them. These organisms were then called “amitochondriates”. More recent studies of evolution and cell biology of “amitochondriate” protists, however, challenged their amitochondriate status, at least in the case of those organis ...
... inhabit oxygen-poor environments, do not possess mitochondria as we know them. These organisms were then called “amitochondriates”. More recent studies of evolution and cell biology of “amitochondriate” protists, however, challenged their amitochondriate status, at least in the case of those organis ...
Pavel Doležal
... inhabit oxygen-poor environments, do not possess mitochondria as we know them. These organisms were then called “amitochondriates”. More recent studies of evolution and cell biology of “amitochondriate” protists, however, challenged their amitochondriate status, at least in the case of those organis ...
... inhabit oxygen-poor environments, do not possess mitochondria as we know them. These organisms were then called “amitochondriates”. More recent studies of evolution and cell biology of “amitochondriate” protists, however, challenged their amitochondriate status, at least in the case of those organis ...
Microbial characterization of white mats in a hydrothermally
... generate chemical disequilibria. Chemolithotrophs, which represent the close link between the biosphere and the lithosphere, take advantage of these disequilibria to obtain energy through the coupling of redox reactions. For example, Halothiobacillus kellyi, isolat ...
... generate chemical disequilibria. Chemolithotrophs, which represent the close link between the biosphere and the lithosphere, take advantage of these disequilibria to obtain energy through the coupling of redox reactions. For example, Halothiobacillus kellyi, isolat ...
Protein reutilisation in corms of Colchicum autumnale
... different proteolytic systems: vacuolar proteolysis, selective nuclear and cytosolic proteolysis, and organellar proteolysis. Because each cellular compartment is af- ...
... different proteolytic systems: vacuolar proteolysis, selective nuclear and cytosolic proteolysis, and organellar proteolysis. Because each cellular compartment is af- ...