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Characterization of Complementary DNA Encoding the Precursor for
Characterization of Complementary DNA Encoding the Precursor for

... predicted peptide sequence that contains a significant block of homology to mGAP, within a region which itself is highly conserved among the mammalian species. To assure that the isolated cichlid cDNA did not represent a cloning artifact in this region, three independent oligo(dT)-primed reverse tra ...
Chloroplast phosphoglycerate kinase from Euglena gracilis
Chloroplast phosphoglycerate kinase from Euglena gracilis

... was replaced by Asn in the third PGK protein at the 3¢ end. At the nucleotide level sequence identity of the PGK segments is 97–99%. The calculated Mr of the deduced amino acid sequence is 44 475 Da, which is in reasonably good agreement with the Mr of 48 kDa estimated from SDS/PAGE (Fig. 1). All th ...
(Rev. 9/04), Biographical Sketch Format Page
(Rev. 9/04), Biographical Sketch Format Page

... Patikoglou, G.A. and Koelle, M.R. (2002). An N-terminal region of C. elegans RGS proteins EGL10 and EAT-16 directs inhibition of Go versus Gq signaling. J. Biol. Chem. 277, 47004-13. Bany, I.A., Dong, M.Q., and Koelle, M.R. (2003). Genetic and cellular basis for acetylcholine inhibition of Caenorh ...
heartsprotein.adv.pdf
heartsprotein.adv.pdf

... Proteins are composed of long chains of amino acids. The order in which these different amino acids appear will determine the 3 dimensional shape of the protein. Interactions between the different R groups will cause the protein to assume and maintain a specific structure. When proteins fold, differ ...
Interdependence, Reflexivity, Fidelity, Impedance Matching
Interdependence, Reflexivity, Fidelity, Impedance Matching

... information has been irreversibly transformed. Any molecular machine charged with reversing translation by unfolding, then “reading” the sequence of a protein would require shuttling each successive amino acid through ~20 active sites until one fitted, and then overcoming the redundancy of the gen ...
Interdependence, Reflexivity, Fidelity, Impedance Matching
Interdependence, Reflexivity, Fidelity, Impedance Matching

... information has been irreversibly transformed. Any molecular machine charged with reversing translation by unfolding, then “reading” the sequence of a protein would require shuttling each successive amino acid through ~20 active sites until one fitted, and then overcoming the redundancy of the gen ...
Sequence Queries
Sequence Queries

... © 2007-2010 Matrix Science ...
Judge, P.J. and Watts, A.
Judge, P.J. and Watts, A.

... tuned to provide specialised functionality. Although a number of biophysical techniques including X-ray crystallography have been used to determine membrane protein structures, these methods are unable to replicate and accommodate the complexity and diversity of natural membranes. Solid state NMR (s ...
Coarse-Grained Modeling of ProteinDynamics
Coarse-Grained Modeling of ProteinDynamics

... The most direct computational approach to protein dynamics prediction is simulation of a dynamic system of interest. A simplified simulation model of proteins is probably the earliest example of CG approach in structural biology, developed in the mid-1970s (Levitt and Warshel 1975). Since that time ...
Plasma Amino Acid Response to Graded Levels of Escape Protein
Plasma Amino Acid Response to Graded Levels of Escape Protein

... meal (FTH; n = 30) were fed at graded levels to obtain growth and plasma amino acid response curves for the different proteins fed. The FTH supplement was 54.2% CP, compared with 29.1% CP for DDG and H-DDG supplements (DM basis). Feather meal protein was fed at higher levels to determine whether inc ...
Increasing the thermostability of sucrose
Increasing the thermostability of sucrose

... variant enzymes are frequently expressed at a lower level than the wild-type enzyme, even when just a single amino acid has been mutated (Tokuriki and Tawfik, 2009a). This problem can be circumvented by creating a small number of site-directed variants that can be processed manually to eliminate the ...
State of the Art Manufacturing of Protein Hydrolysates
State of the Art Manufacturing of Protein Hydrolysates

... of salt, which is detrimental to the growth of ­microorganisms. For this reason some of the manufacturers remove salt partially or completely by precipitation nanofiltration and/or ion exchange resins. However, acid hydrolysates are widely used in the food and pet food industry as flavor enhancers ( ...
PDF - Fred Hutchinson Cancer Research Center
PDF - Fred Hutchinson Cancer Research Center

... Fast Track ...
Redalyc.MOLECULAR CHARACTERIZATION OF CRUDE SEED
Redalyc.MOLECULAR CHARACTERIZATION OF CRUDE SEED

... S-S links, as indicated by García et al. (2010), who found a trimer with units of ~10kDa. Dörries (2005) suggested the presence of other bands, corresponding to isoforms of the same protein, indicating the presence of proteins of higher molecular weight, only present during the first two days. In Fi ...
Journal of the Science of Food and Agriculture
Journal of the Science of Food and Agriculture

... disease. Proteins from wheat, rice, maize and barley may give rise to allergic reactions such as atopic dermatitis and asthma.1 These components are not found in pseudocereals and legumes such as amaranth and soybean. Therefore these plants might be used as substitutes for cereals in gluten-free die ...
DO ANTIBODIES RECOGNIZE AMINO ACID SIDE CHAINS OF
DO ANTIBODIES RECOGNIZE AMINO ACID SIDE CHAINS OF

... orientation . Synthetic peptide inhibition experiments confirmed the ability of a reversed sequence to resemble the nominal antigenic epitope . In contrast to earlier studies in which internal image determinants were present in the expected orientation (7, 8), the results presented here suggest that ...
molecular dynamics studies on mammalian apometallothioneins
molecular dynamics studies on mammalian apometallothioneins

... evolved. Despite innumerable publications on MT, the literature on apo-MT is limited. The in vitro stability of apo-MTs is the main hurdle in their spectroscopic characterization and primary reason for the little attention paid to them. Only in last decade, fluorescence based methods have been devel ...
Are You suprised ?
Are You suprised ?

... among organisms. Proteins consist of chains of amino acids. The sequence, or order, of the amino acids in a protein determines the type and nature of the protein. In turn, the sequence of amino acids in a protein is determined by the sequence of nucleotides in a gene. A change in the DNA nucleotide ...
Adaptations of protein structure and function to temperature: there is
Adaptations of protein structure and function to temperature: there is

... living at 0°C does not have a metabolic rate 20-times lower than that of a desert lizard living at 40°C. Thus, there must be a compensatory mechanism available, acting on an evolutionary time scale, that allows natural selection to alter rates of metabolic reactions as organisms adapt to new environ ...
Electrophoretic Extraction and Proteomic Characterization of
Electrophoretic Extraction and Proteomic Characterization of

... Much of the ocean is influenced by nitrogen limitation [1], and thus, understanding marine protein cycling is important for tracking the global organic nitrogen cycle. Solid state NMR has provided evidence that the majority of organic nitrogen in dissolved and particulate marine organic matter conta ...
novel 4E-interacting protein in Leishmania is involved in stage
novel 4E-interacting protein in Leishmania is involved in stage

... Leishmania are unicellular protozoans with a complex life cycle. They reside as flagellated promastigotes in the alimentary canal of sand-fly vectors. After being transmitted into the mammalian host through a blood-meal from the female vector, they are engulfed by macrophages and other cells of the im ...
PDF Format - Kinexus Bioinformatics Corporation
PDF Format - Kinexus Bioinformatics Corporation

... kinase specificity matrices, which identify the optimal phosphosite amino acid sequences for substrate recognition, are available with our In Silico Kinase Specificity Prediction (IKSP) services. As long as a typical protein kinase from any eukaryotic species is identified with a Uniprot number, we ...
Protein for Athletes
Protein for Athletes

... ‘‘there is a linear relationship between the amount of protein ingested and resulting desirable adaptation of muscle.’’5 The bottom line is that, at present, we cannot pinpoint the exact protein needs of athletes with any ...
X-ray structures of the N and C-terminal domains of a
X-ray structures of the N and C-terminal domains of a

... The N protein is a multifunctional phosphoprotein with a molecular mass ranging between 45 and 60 kDa in the various groups of coronaviruses, which, along with its coding RNA, is synthesized in large amounts during infection (20, 39). The highly basic N protein is able to bind ssRNA non-specificall ...
Cyanuric acid hydrolase: evolutionary innovation by structural
Cyanuric acid hydrolase: evolutionary innovation by structural

... (Ser85–Lys42) is hypothesized, based on biochemical evidence and crystallographic data. A plausible catalytic mechanism based on these observations is also presented. A comparison with a homology model of the related barbiturase, Bar, was used to infer the active-site residues responsible for substr ...
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Ancestral sequence reconstruction

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