PhytoREF: a reference database of the plastidial 16S
... obtained in these studies have been hindered by the lack of reference sequences of taxonomically well-identified organisms. Although a number of curated reference databases are publicly available for ribosomal RNA genes of eukaryotes and prokaryotes, such as the Protist Ribosomal Reference Database ...
... obtained in these studies have been hindered by the lack of reference sequences of taxonomically well-identified organisms. Although a number of curated reference databases are publicly available for ribosomal RNA genes of eukaryotes and prokaryotes, such as the Protist Ribosomal Reference Database ...
Interacting specificity of a histidine kinase and its cognate response
... sequence upstream of the prrA start codon in addition to the entire prrA gene. Since this additional DNA sequence does not contain a stop codon that lies in the same reading frame as the GAL4AD and prrA genes, the PrrA protein expressed from pPLAC (pGADT7 : : prrA) has an additional 28 amino acid re ...
... sequence upstream of the prrA start codon in addition to the entire prrA gene. Since this additional DNA sequence does not contain a stop codon that lies in the same reading frame as the GAL4AD and prrA genes, the PrrA protein expressed from pPLAC (pGADT7 : : prrA) has an additional 28 amino acid re ...
power-point slides
... CENTER FOR BIOLOGICAL SEQUENCE ANALYSIS TECHNICAL UNIVERSITY OF DENMARK DTU ...
... CENTER FOR BIOLOGICAL SEQUENCE ANALYSIS TECHNICAL UNIVERSITY OF DENMARK DTU ...
Small-angle scattering studies of intrinsically disordered proteins
... proteins. In order to fully exploit the structural and dynamic information encoded in SAS data, it is necessary to use realistic three-dimensional (3D) models. However, the generation of conformational ensembles of disordered proteins is extremely challenging, mainly because of the flat energy lands ...
... proteins. In order to fully exploit the structural and dynamic information encoded in SAS data, it is necessary to use realistic three-dimensional (3D) models. However, the generation of conformational ensembles of disordered proteins is extremely challenging, mainly because of the flat energy lands ...
Minimal domain of bacterial phytochrome required for chromophore binding and fluorescence
... Fluorescent proteins (FP) are used to study various biological processes. Recently, a series of nearinfrared (NIR) FPs based on bacterial phytochromes was developed. Finding ways to improve NIR FPs is becoming progressively important. By applying rational design and molecular evolution we have engin ...
... Fluorescent proteins (FP) are used to study various biological processes. Recently, a series of nearinfrared (NIR) FPs based on bacterial phytochromes was developed. Finding ways to improve NIR FPs is becoming progressively important. By applying rational design and molecular evolution we have engin ...
STRUCTURE AND ALLERGENICITY OF WHEAT GLUTEN PROTEINS
... A different structural model exists for the highest molecular weight gliadins. Omega-gliadins contain short, distinct C and N terminal sequences, separated by a region of repeatable sequences similar to γ gliadins. The sequence differs from that of γ gliadins, however, in having additional glutamine ...
... A different structural model exists for the highest molecular weight gliadins. Omega-gliadins contain short, distinct C and N terminal sequences, separated by a region of repeatable sequences similar to γ gliadins. The sequence differs from that of γ gliadins, however, in having additional glutamine ...
Lipid–protein interactions probed by electron crystallography
... had significantly lower B-factor values when they were coupled to lipids rather than to detergents. This was attributed to lipid stabilizing interactions in the 2D crystals, suggesting that even nonspecific lipid–protein interactions have a significant stabilizing effect on membrane proteins [17]. ...
... had significantly lower B-factor values when they were coupled to lipids rather than to detergents. This was attributed to lipid stabilizing interactions in the 2D crystals, suggesting that even nonspecific lipid–protein interactions have a significant stabilizing effect on membrane proteins [17]. ...
X-ray structures of the N and C-terminal domains of a
... 1.3 Å resolution, is similar to the previously published Beaudette strain (12) but makes strikingly different quaternary associations. We describe the first crystal structure of the CTD dimerization domain of the coronavirus N protein at a resolution of 2.2 Å. Our X-ray crystallographic analysis of ...
... 1.3 Å resolution, is similar to the previously published Beaudette strain (12) but makes strikingly different quaternary associations. We describe the first crystal structure of the CTD dimerization domain of the coronavirus N protein at a resolution of 2.2 Å. Our X-ray crystallographic analysis of ...
april7_maindocument_jvirol
... 1.3 Å resolution, is similar to the previously published Beaudette strain (12) but makes strikingly different quaternary associations. We describe the first crystal structure of the CTD dimerization domain of the coronavirus N protein at a resolution of 2.2 Å. Our X-ray crystallographic analysis of ...
... 1.3 Å resolution, is similar to the previously published Beaudette strain (12) but makes strikingly different quaternary associations. We describe the first crystal structure of the CTD dimerization domain of the coronavirus N protein at a resolution of 2.2 Å. Our X-ray crystallographic analysis of ...
BLAST: Basic Local Alignment Search Tool Meng Cao, Arthur Lee
... behind the algorithm is that it searches for segments of DNA that are likely to be the most similar, rather than comparing each individual section with every other one. This innovation increases the speed with which DNA can be searched, but is not perfect. It is possible that BLAST will return data ...
... behind the algorithm is that it searches for segments of DNA that are likely to be the most similar, rather than comparing each individual section with every other one. This innovation increases the speed with which DNA can be searched, but is not perfect. It is possible that BLAST will return data ...
Inositol 1,3,4,5,6-Pentakisphosphate 2-Kinase
... copy in the genome, hybridization with a 32P-labeled cDNA probe (as shown in Fig. 2) should detect only one band larger than 9 kb. As shown in Figure 3, two restriction digestion fragments were able to hybridize with ZmIPK1 cDNA probe when the genomic DNA was digested with EcoRI, including a band sm ...
... copy in the genome, hybridization with a 32P-labeled cDNA probe (as shown in Fig. 2) should detect only one band larger than 9 kb. As shown in Figure 3, two restriction digestion fragments were able to hybridize with ZmIPK1 cDNA probe when the genomic DNA was digested with EcoRI, including a band sm ...
Origin of Life
... explanations. There is nothing inferior about this, in fact, it is deductive reasoning which is used by scientists all the time in their quest for truth. Arguments for a Designer generally revolve around probability. Meaningful complexity is unlikely to result from random events. Organisms are meani ...
... explanations. There is nothing inferior about this, in fact, it is deductive reasoning which is used by scientists all the time in their quest for truth. Arguments for a Designer generally revolve around probability. Meaningful complexity is unlikely to result from random events. Organisms are meani ...
proposal-aug25
... represent a substantial contribution and will be generally applicable beyond the scope of this project. Why an evolutionary approach for disordered regions? For the vast majority of intrinsically disordered regions, specific biological functions remain unknown. Computational methods can readily iden ...
... represent a substantial contribution and will be generally applicable beyond the scope of this project. Why an evolutionary approach for disordered regions? For the vast majority of intrinsically disordered regions, specific biological functions remain unknown. Computational methods can readily iden ...
The Action of Chloramphenicol on Protein and Nucleic Acid
... workers and those reported here, an experiment was carried out under conditions similar to those described by Wisseman et al. (1954).This involved the incubation of the organisms, after harvesting, in medium without chloramphenicol ‘until the logarithmic rate of growth was well established’ (90 min. ...
... workers and those reported here, an experiment was carried out under conditions similar to those described by Wisseman et al. (1954).This involved the incubation of the organisms, after harvesting, in medium without chloramphenicol ‘until the logarithmic rate of growth was well established’ (90 min. ...
The Action of Chloramphenicol on Protein and Nucleic Acid
... workers and those reported here, an experiment was carried out under conditions similar to those described by Wisseman et al. (1954).This involved the incubation of the organisms, after harvesting, in medium without chloramphenicol ‘until the logarithmic rate of growth was well established’ (90 min. ...
... workers and those reported here, an experiment was carried out under conditions similar to those described by Wisseman et al. (1954).This involved the incubation of the organisms, after harvesting, in medium without chloramphenicol ‘until the logarithmic rate of growth was well established’ (90 min. ...
Proteins
... as we can synthesize them in our bodies. The variable (R) group is this is the only part of the 20 amino acids in human nutrition that is different and unique to each. This side group (R) can be basic, acidic, polar, non-polar, charged, or neutral. The way this side group interacts with other side g ...
... as we can synthesize them in our bodies. The variable (R) group is this is the only part of the 20 amino acids in human nutrition that is different and unique to each. This side group (R) can be basic, acidic, polar, non-polar, charged, or neutral. The way this side group interacts with other side g ...
Protein Synthesis and Words - Hewlett
... Objective: Follow the steps of protein synthesis, Understand how genes are expressed. Background: DNA carries and stores the information for the synthesis of all of the proteins of an organism. We commonly call this our “traits”. Protein molecules are large and complex, composed of hundreds of amino ...
... Objective: Follow the steps of protein synthesis, Understand how genes are expressed. Background: DNA carries and stores the information for the synthesis of all of the proteins of an organism. We commonly call this our “traits”. Protein molecules are large and complex, composed of hundreds of amino ...
Large-scale analysis of Influenza A virus nucleoprotein sequence
... the multiple sequence alignment and the phylogenetic tree calculated with the FLU substitution model (Cuong et al., 2010) were uploaded manually and the Bayesian statistic method was chosen. The resulting conservation scores are standard scores with an average of zero and a standard deviation of one ...
... the multiple sequence alignment and the phylogenetic tree calculated with the FLU substitution model (Cuong et al., 2010) were uploaded manually and the Bayesian statistic method was chosen. The resulting conservation scores are standard scores with an average of zero and a standard deviation of one ...
Bioinformatics
... – 1 PAM is the amount of evolutionary change that yields, on average, one substitution in 100 amino acid residues – A positive score signifies a common replacement whereas a negative score signifies an unlikely replacement – PAM250 matrix assumes/is optimized for sequences separated by 250 PAM, i.e. ...
... – 1 PAM is the amount of evolutionary change that yields, on average, one substitution in 100 amino acid residues – A positive score signifies a common replacement whereas a negative score signifies an unlikely replacement – PAM250 matrix assumes/is optimized for sequences separated by 250 PAM, i.e. ...
Proteomics of spermatogenesis: from protein lists to understanding
... were upregulated during mouse spermatogenesis, and the highest expression level was found in spermatozoa. The vertical axis indicates expression levels of these proteins in our studies. The horizontal axis indicates different time points of mouse development stages. d, day. ...
... were upregulated during mouse spermatogenesis, and the highest expression level was found in spermatozoa. The vertical axis indicates expression levels of these proteins in our studies. The horizontal axis indicates different time points of mouse development stages. d, day. ...
HiFliQ Ni-NTA FPLC Columns - User Guide
... cell pellet under native conditions using Protein Ark HiFliQ Ni-NTA FPLC Columns. Reagent amounts given apply to IPTG-induced bacterial culture of a well-expressed protein (approximately 10-50 mg/l). Cells are lysed with lysozyme because it is an inexpensive and efficient method for cells that have ...
... cell pellet under native conditions using Protein Ark HiFliQ Ni-NTA FPLC Columns. Reagent amounts given apply to IPTG-induced bacterial culture of a well-expressed protein (approximately 10-50 mg/l). Cells are lysed with lysozyme because it is an inexpensive and efficient method for cells that have ...
NF96-251 A Comparative Study of Fiber Digestion and Subsequent
... minerals has occurred in the small intestine. Because the site of fiber digestion is after the small intestine, this means that little to no protein or amino acid utilization can occur during the microbial forage breakdown. Protein and amino acids are basically only absorbed in the small intestine w ...
... minerals has occurred in the small intestine. Because the site of fiber digestion is after the small intestine, this means that little to no protein or amino acid utilization can occur during the microbial forage breakdown. Protein and amino acids are basically only absorbed in the small intestine w ...
The extraction of collagen protein from pigskin
... separation the solution of collagen protein is obtained. The dechroming fraction of alkali method is higher than that of acid method, but it has some disadvantages as well. For example, the hydrolysis level of the obtained collagen protein is high; the molecular weight is small; ash content and chro ...
... separation the solution of collagen protein is obtained. The dechroming fraction of alkali method is higher than that of acid method, but it has some disadvantages as well. For example, the hydrolysis level of the obtained collagen protein is high; the molecular weight is small; ash content and chro ...
In-Lab Handout
... Methionine, alanine, leucine, glutamic acid, and lysine ("MALEK" in the amino-acid 1letter codes) all have high helix-forming propensities. Proline (“P”) tends to break or kink helices. However, proline is often seen as the first residue of a helix. (10e) Write a regex for an amino acid pattern that ...
... Methionine, alanine, leucine, glutamic acid, and lysine ("MALEK" in the amino-acid 1letter codes) all have high helix-forming propensities. Proline (“P”) tends to break or kink helices. However, proline is often seen as the first residue of a helix. (10e) Write a regex for an amino acid pattern that ...
PROTEIN-LIPID AND PROTEIN-CARBOHYDRATE INTERACTIONS
... way of covalent bonding between carbohydrates and protein side chains via phenolic compounds. Experiments made recently in our laboratory have shown that hydrophilic, gel-forming carbohydrates (plant gums, pectic substances, etc.) added in small quantity (1 - 2%) improved the dough rheologi}:al prop ...
... way of covalent bonding between carbohydrates and protein side chains via phenolic compounds. Experiments made recently in our laboratory have shown that hydrophilic, gel-forming carbohydrates (plant gums, pectic substances, etc.) added in small quantity (1 - 2%) improved the dough rheologi}:al prop ...