Stabilization by GroEL, a Molecular Chaperone, and a Periplasmic
... only a few such proteins are known. Furthermore, the periplasm of Escherichia coli has been of great interest with respect to the functional expression of a wide variety of recombinant proteins from different sources. However, the periplasmic folding of proteins has not been studied in any great det ...
... only a few such proteins are known. Furthermore, the periplasm of Escherichia coli has been of great interest with respect to the functional expression of a wide variety of recombinant proteins from different sources. However, the periplasmic folding of proteins has not been studied in any great det ...
E-mail: - HAL
... Many research groups have designed fragment libraries or structural alphabets to try to describe the local structural features of known protein structures more accurately.17-29 These libraries or alphabets correspond to finite sets of protein structural fragments. They can be differentiated accordin ...
... Many research groups have designed fragment libraries or structural alphabets to try to describe the local structural features of known protein structures more accurately.17-29 These libraries or alphabets correspond to finite sets of protein structural fragments. They can be differentiated accordin ...
Building proteins from C, coordinates using the dihedral probability
... may enable complete protein structures to be built from the C, coordinates of a lattice representation. Our work was motivated by both of these factors: the desire to build full protein structures from lattice structures, and to provide a means for evaluating different lattice conformations. In addi ...
... may enable complete protein structures to be built from the C, coordinates of a lattice representation. Our work was motivated by both of these factors: the desire to build full protein structures from lattice structures, and to provide a means for evaluating different lattice conformations. In addi ...
structural basis for thermal stability of thermophilic trmd proteins
... and that protein stability was of course an obvious factor to consider in thermostability. A protein‟s three dimensional structure is known to be very complex involving various inter and intra molecular bonds. The very same interactions present in mesophilic proteins can be found in thermophilic pro ...
... and that protein stability was of course an obvious factor to consider in thermostability. A protein‟s three dimensional structure is known to be very complex involving various inter and intra molecular bonds. The very same interactions present in mesophilic proteins can be found in thermophilic pro ...
Analysis of a ribose transport operon from Bacillus
... these systems have a protein homologous to the periplasmic substrate-binding protein, even though this bacterium does not have a periplasm. Nevertheless, Perego e t al. (1991) have demonstrated that the periplasmic oligopeptide binding protein OppA is cell wall associated in exponentially growing ce ...
... these systems have a protein homologous to the periplasmic substrate-binding protein, even though this bacterium does not have a periplasm. Nevertheless, Perego e t al. (1991) have demonstrated that the periplasmic oligopeptide binding protein OppA is cell wall associated in exponentially growing ce ...
Protein Engineering in the Development of
... of hybrid hydrogels in response to binding events (15, 48–50). Nature is rich with diversity: There are many protein domains and peptide motifs with interesting ligand binding properties, with structures that undergo conformation changes, and that have complex functions yet to be used in responsive ...
... of hybrid hydrogels in response to binding events (15, 48–50). Nature is rich with diversity: There are many protein domains and peptide motifs with interesting ligand binding properties, with structures that undergo conformation changes, and that have complex functions yet to be used in responsive ...
Crude protein and amino acids content in some common
... for 24 hours. The samples were then cooled at room temperature for 20 minutes. A total of 400 µL of alpha amino butyric acid (AABA) was added into sample and filtered with Sartorius stedim filter paper (Biotech, 1288) into 100 mL flask. The samples were then diluted with deionized water and then fil ...
... for 24 hours. The samples were then cooled at room temperature for 20 minutes. A total of 400 µL of alpha amino butyric acid (AABA) was added into sample and filtered with Sartorius stedim filter paper (Biotech, 1288) into 100 mL flask. The samples were then diluted with deionized water and then fil ...
Heptad repeat sequences are located adjacent to hydrophobic
... which two or more a-helices associate lengthwise by coiling around each other to maintain contact along hydrophobic faces (Cohen & Philips, 1981; Cohen & Parry, 1986). Coiled coils may form in proteins where extensive regions that are devoid of potential helixbreaking prolines occur, and consist of ...
... which two or more a-helices associate lengthwise by coiling around each other to maintain contact along hydrophobic faces (Cohen & Philips, 1981; Cohen & Parry, 1986). Coiled coils may form in proteins where extensive regions that are devoid of potential helixbreaking prolines occur, and consist of ...
Cloning and sequencing of a gene encoding acidophilic amylase
... 90 kDa form. The amount of protein in the 90 kDa and 160 kDa bands was approximately the same, yet the starch-degrading activity of the 160 kDa protein was much higher (Fig. 1). Thus, the 160 kDa form appears to have a much higher specific activity, assuming that both proteins are refolded with simi ...
... 90 kDa form. The amount of protein in the 90 kDa and 160 kDa bands was approximately the same, yet the starch-degrading activity of the 160 kDa protein was much higher (Fig. 1). Thus, the 160 kDa form appears to have a much higher specific activity, assuming that both proteins are refolded with simi ...
VGIchan: Prediction and Classification of Voltage-Gated Ion
... Fig. 3 Snapshot of the results obtained after the analysis of submission. ...
... Fig. 3 Snapshot of the results obtained after the analysis of submission. ...
Heptad repeat sequences are located adjacent to hydrophobic
... which two or more a-helices associate lengthwise by coiling around each other to maintain contact along hydrophobic faces (Cohen & Philips, 1981; Cohen & Parry, 1986). Coiled coils may form in proteins where extensive regions that are devoid of potential helixbreaking prolines occur, and consist of ...
... which two or more a-helices associate lengthwise by coiling around each other to maintain contact along hydrophobic faces (Cohen & Philips, 1981; Cohen & Parry, 1986). Coiled coils may form in proteins where extensive regions that are devoid of potential helixbreaking prolines occur, and consist of ...
Dictionary of Interfaces in Proteins (DIP). Data Bank of
... 1990). A similar classi®cation of different proteinprotein interactions was recently carried out by Jones & Thornton (1996). Recent studies of protein folding (e.g. see Peng et al., 1995) indicate that molten globules do have major secondary structures resembling native topology. These results sugge ...
... 1990). A similar classi®cation of different proteinprotein interactions was recently carried out by Jones & Thornton (1996). Recent studies of protein folding (e.g. see Peng et al., 1995) indicate that molten globules do have major secondary structures resembling native topology. These results sugge ...
Serine phosphorylation of the cotton cytosolic pyruvate kinase
... (Amersham Biosciences, Uppsala, Sweden) with a continuous recording function. The Vmax values were automatically calculated by the spectrophotometer. ...
... (Amersham Biosciences, Uppsala, Sweden) with a continuous recording function. The Vmax values were automatically calculated by the spectrophotometer. ...
Thermostable glycerol kinase from a
... after sonication lysis (crude lysate) was comparable to that in the whole cell extract, the enzyme should be accumulated in cells in a soluble form (data not shown). From the crude lysate, the enzyme was purified in only two steps with a yield of 30–40% (Table I). The purified enzyme gave a single b ...
... after sonication lysis (crude lysate) was comparable to that in the whole cell extract, the enzyme should be accumulated in cells in a soluble form (data not shown). From the crude lysate, the enzyme was purified in only two steps with a yield of 30–40% (Table I). The purified enzyme gave a single b ...
SpeeDB: fast structural protein searches
... structures from Protein Data Bank (PDB). Although these tools allow users to identify and analyze interactions, analysis can only be performed on one structure at a time. This makes it difficult and time consuming to study the significance of these interactions on a large scale. Results: SpeeDB is a ...
... structures from Protein Data Bank (PDB). Although these tools allow users to identify and analyze interactions, analysis can only be performed on one structure at a time. This makes it difficult and time consuming to study the significance of these interactions on a large scale. Results: SpeeDB is a ...
Statistical Analysis of Amino Acid Patterns in Transmembrane
... pairs of residues at distance i, i k (k is also referred to as the register). For example, the pair in which Ala and Leu are at i, i 3 (AxxL) is indicated by AL3. The occurrences in the TM sequence database of all 4000 pairs formed by all combinations of the 20 amino acids at registers 1 to 10 w ...
... pairs of residues at distance i, i k (k is also referred to as the register). For example, the pair in which Ala and Leu are at i, i 3 (AxxL) is indicated by AL3. The occurrences in the TM sequence database of all 4000 pairs formed by all combinations of the 20 amino acids at registers 1 to 10 w ...
PC235: 2008 Lecture 5 Quantitation
... •Coomassie and Sypro stain densities are relatively linear with respect to amount. •Silver stain is less quantitative. •Complication: How do you know there is only one major protein component in the band. •Greater separation with 2D gels reduces chances of multiple components per spot •Multiple comp ...
... •Coomassie and Sypro stain densities are relatively linear with respect to amount. •Silver stain is less quantitative. •Complication: How do you know there is only one major protein component in the band. •Greater separation with 2D gels reduces chances of multiple components per spot •Multiple comp ...
Low Protein Diet - Hamilton Health Sciences
... Your child has been diagnosed with a metabolic disorder that changes the way some amino acids are used in his or her body. Certain amino acids are able to build up in the blood and cause harm. This is because an enzyme in your child’s body is not working properly or is missing. ...
... Your child has been diagnosed with a metabolic disorder that changes the way some amino acids are used in his or her body. Certain amino acids are able to build up in the blood and cause harm. This is because an enzyme in your child’s body is not working properly or is missing. ...
8/18/2015 1 BCMB 3100
... Work with your group of 4 people and find at least one example of a mutation in humans, or in industryrelevant plants, animals or microbes. Present, on a single, one-side, typed page, the amino acid mutated, the phenotype of the effect on the organism, the molecular reason that the mutation causes t ...
... Work with your group of 4 people and find at least one example of a mutation in humans, or in industryrelevant plants, animals or microbes. Present, on a single, one-side, typed page, the amino acid mutated, the phenotype of the effect on the organism, the molecular reason that the mutation causes t ...
Notes: Amino Acids and Proteins:
... eggs) provide what's called "complete protein," meaning that they contain all of the essential amino acids. Vegetable sources usually are low on or missing certain essential amino acids. For example, rice is low in isoleucine and lysine. However, different vegetable sources are deficient in differen ...
... eggs) provide what's called "complete protein," meaning that they contain all of the essential amino acids. Vegetable sources usually are low on or missing certain essential amino acids. For example, rice is low in isoleucine and lysine. However, different vegetable sources are deficient in differen ...
Griffith_155
... weights calculated from the sequences of the enzymes (e.g., 34,466 for the B. cereus PI-PLC). The value is higher than that observed by gel filtration ...
... weights calculated from the sequences of the enzymes (e.g., 34,466 for the B. cereus PI-PLC). The value is higher than that observed by gel filtration ...
Protein and DNA sequence determinants of
... As proteins and nucleic acids must remain in their native conformations at physiologically relevant temperatures, thermal adaptation requires adjustment of interactions within these biopolymers. Given the limited alphabet of amino acid residues, an apparent way to control protein stability is to pro ...
... As proteins and nucleic acids must remain in their native conformations at physiologically relevant temperatures, thermal adaptation requires adjustment of interactions within these biopolymers. Given the limited alphabet of amino acid residues, an apparent way to control protein stability is to pro ...
HiTrap Chelating HP 1 ml and 5 ml
... coupled to the Sepharose High Performance matrix by stable ether bonds via a seven-atom spacer arm. This gives a very stable adsorbent that can be used over the pH range 4–12. When charged with a suitable metal ion, HiTrap Chelating HP will selectively retain proteins if complex-forming amino acid r ...
... coupled to the Sepharose High Performance matrix by stable ether bonds via a seven-atom spacer arm. This gives a very stable adsorbent that can be used over the pH range 4–12. When charged with a suitable metal ion, HiTrap Chelating HP will selectively retain proteins if complex-forming amino acid r ...
reconstructing ancestral bioclimatic models on phylogenetic trees
... • Are Drosera radiations linked with this climate change? BiodiversityWorld GRID Workshop NeSC, Edinburgh – 30 June and 1 July 2005 ...
... • Are Drosera radiations linked with this climate change? BiodiversityWorld GRID Workshop NeSC, Edinburgh – 30 June and 1 July 2005 ...
Noncovalently Associated Complexes: Apomyoglobin and
... ionization/mass spectrometry (ESI/MS) has recently been utilized to study noncovalently associated complexes (1, 2). In general, picomoles or less of a protein complex are required for analysis by ESI/MS and analysis times using infusion for sample introduction are only a few minutes. The low-energy ...
... ionization/mass spectrometry (ESI/MS) has recently been utilized to study noncovalently associated complexes (1, 2). In general, picomoles or less of a protein complex are required for analysis by ESI/MS and analysis times using infusion for sample introduction are only a few minutes. The low-energy ...