Crystallization and X Ray Diffraction Data Analyses of the Enzyme
... (TcUH) three-dimensional structure by X ray crystallography will aggregate valuable information per se, such as the better understanding of the enzyme at molecular level and its catalytic mechanism, which should also be compared to homologous proteins. This information, added to further molecular bi ...
... (TcUH) three-dimensional structure by X ray crystallography will aggregate valuable information per se, such as the better understanding of the enzyme at molecular level and its catalytic mechanism, which should also be compared to homologous proteins. This information, added to further molecular bi ...
Biology - Kenyon College
... For two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (2-D gels), growth media contained LBK broth (10 g of tryptone, 5 g of yeast extract, 7.45 g of KCl) or M63 salts [3 g of KH2PO4, 7 g of K2HPO4, 2 g of (NH4)2SO4, 0.5 ml of FeSO4 at 1 mg/ml, 2 ml of 0.5 M MgSO2, 100 mg of ...
... For two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (2-D gels), growth media contained LBK broth (10 g of tryptone, 5 g of yeast extract, 7.45 g of KCl) or M63 salts [3 g of KH2PO4, 7 g of K2HPO4, 2 g of (NH4)2SO4, 0.5 ml of FeSO4 at 1 mg/ml, 2 ml of 0.5 M MgSO2, 100 mg of ...
Predicting DNA-binding sites of proteins from amino acid sequence
... More recently, methods for precisely identifying proteinDNA contacts by coupling photochemical crosslinking with mass spectrometry have also been developed [9]. With increasing availability of protein sequence data, there is an urgent need for computational tools that can rapidly and reliably identi ...
... More recently, methods for precisely identifying proteinDNA contacts by coupling photochemical crosslinking with mass spectrometry have also been developed [9]. With increasing availability of protein sequence data, there is an urgent need for computational tools that can rapidly and reliably identi ...
IMGT-ONTOLOGY and IMGT databases, tools and Web
... allows extraction and comparison of data for the complex B and T cell antigen receptors. The CLASSIFICATION concept (Fig. 2) has been used to set up a unique nomenclature of the human IG and TR genes, which was approved by the Human Genome Organization (HUGO) Nomenclature Committee (HGNC) in 1999 (W ...
... allows extraction and comparison of data for the complex B and T cell antigen receptors. The CLASSIFICATION concept (Fig. 2) has been used to set up a unique nomenclature of the human IG and TR genes, which was approved by the Human Genome Organization (HUGO) Nomenclature Committee (HGNC) in 1999 (W ...
The sequence of human serum albumin cDNA and its expression in
... the construction of the mature HSA expression plasmid, this cDNA clone (P-14) allowed determination of the DNA sequence of the "prepro" peptide coding and 5' non-coding regions of HSA mRNA. The mature HSA mRNA sequence was joined to a vector plasmid for direct expression of the mature protein in £ . ...
... the construction of the mature HSA expression plasmid, this cDNA clone (P-14) allowed determination of the DNA sequence of the "prepro" peptide coding and 5' non-coding regions of HSA mRNA. The mature HSA mRNA sequence was joined to a vector plasmid for direct expression of the mature protein in £ . ...
Citrate synthase proteins in extremophilic organisms: Studies within
... are less demanding computationally than molecular dynamics simulations, are known to capture local fluctuations about the native state.26 Here, we employ a standard Go-type model to examine thermal stability, local flexibility, collective motions, and unfolding kinetics of CS enzymes functioning in or ...
... are less demanding computationally than molecular dynamics simulations, are known to capture local fluctuations about the native state.26 Here, we employ a standard Go-type model to examine thermal stability, local flexibility, collective motions, and unfolding kinetics of CS enzymes functioning in or ...
Refining the Definition of Plant Mitochondrial
... presequences (Lister and Whelan, 2006). Although structural studies reveal the importance of hydrophobic residues for presequence binding, several studies on yeast, mammals, and plants reveal an important role for positively charged residues in presequences for import into mitochondria (Lister et al ...
... presequences (Lister and Whelan, 2006). Although structural studies reveal the importance of hydrophobic residues for presequence binding, several studies on yeast, mammals, and plants reveal an important role for positively charged residues in presequences for import into mitochondria (Lister et al ...
Kinetic Role of Helix Caps in Protein Folding Is Context
... residues are defined as the first and last residues of the helix with CR atoms within the helical spiral (7) (see Materials and Methods for exact details). Extensive analyses by several groups have shown that helix caps at the N-terminus can stabilize and increase the helicity of model peptides (912 ...
... residues are defined as the first and last residues of the helix with CR atoms within the helical spiral (7) (see Materials and Methods for exact details). Extensive analyses by several groups have shown that helix caps at the N-terminus can stabilize and increase the helicity of model peptides (912 ...
Molecular cloning of Per a 1 and definition of the cross
... Bd90K belong to the same family of Group 1 allergens. These allergens occur in multiple molecular forms with different molecular weights, which are the result of replication of approximately 100 amino acid repeats in the sequences. A detailed analysis of the Bla g 1 repeats and of the homology betwe ...
... Bd90K belong to the same family of Group 1 allergens. These allergens occur in multiple molecular forms with different molecular weights, which are the result of replication of approximately 100 amino acid repeats in the sequences. A detailed analysis of the Bla g 1 repeats and of the homology betwe ...
Modulating the cobalt redox potential through imidazole hydrogen
... the cofactor is replaced by a protein histidine (“His”) anchoring group (“His-on” conguration). This mode of protein-cofactor binding is shown in Fig. 1 (ref. 10) and encountered, amongst others, in MeCbl-dependent methionine synthase (MetH) as well as AdoCbl dependent methylmalonyl CoA mutase (MCM ...
... the cofactor is replaced by a protein histidine (“His”) anchoring group (“His-on” conguration). This mode of protein-cofactor binding is shown in Fig. 1 (ref. 10) and encountered, amongst others, in MeCbl-dependent methionine synthase (MetH) as well as AdoCbl dependent methylmalonyl CoA mutase (MCM ...
Contributions of direct incorporation from diet and microbial amino
... (Ambrose & Norr 1993; Voight et al. 2008), and fish (Kelly & Martinez del Rio 2010). While these experiments demonstrate that routing produces results that deviate from expectations based on mixing models, they were not able to characterize the isotopic patterns among specific amino acids that contr ...
... (Ambrose & Norr 1993; Voight et al. 2008), and fish (Kelly & Martinez del Rio 2010). While these experiments demonstrate that routing produces results that deviate from expectations based on mixing models, they were not able to characterize the isotopic patterns among specific amino acids that contr ...
Discovery of genes in the Pacific oyster (Crassostrea gigas) involved
... prostaglandin E2 production and increased expression of cyclooxygenase mRNA and protein (Ballou et al. ’92). While the role of ceramide as a signaling molecule in response to stress has been well studied in mammalian systems there is little information on the function and metabolism of ceramide in ...
... prostaglandin E2 production and increased expression of cyclooxygenase mRNA and protein (Ballou et al. ’92). While the role of ceramide as a signaling molecule in response to stress has been well studied in mammalian systems there is little information on the function and metabolism of ceramide in ...
Homology Modelling and Structural Comparisons of Capsid
... Similarities between the amino acid sequences of circovirus Cap proteins and the solved PCV2 Cap also allow for models to be constructed. The differences among strains and between viruses may have different effects when comparing their structural attributes. The rapidly growing number of circoviruse ...
... Similarities between the amino acid sequences of circovirus Cap proteins and the solved PCV2 Cap also allow for models to be constructed. The differences among strains and between viruses may have different effects when comparing their structural attributes. The rapidly growing number of circoviruse ...
Metabolic rate depression in animals
... regulatory control that are conserved across phylogenetic lines and that are broadly applied to the control of multiple cell functions. One such mechanism is reversible protein phosphorylation which is now known to contribute to the regulation of fuel metabolism, to ion channel arrest, and to the su ...
... regulatory control that are conserved across phylogenetic lines and that are broadly applied to the control of multiple cell functions. One such mechanism is reversible protein phosphorylation which is now known to contribute to the regulation of fuel metabolism, to ion channel arrest, and to the su ...
Serine/Threonine Phosphatases
... acid; F, phenylalanine; W, tryptophan). This notion was supported by the crystal structure of PP1 bound to a peptide containing the sequence RRVSFA (S, serine; A, alanine) (Egloff et al., 1997). The valine and phenylalanine residues of the peptide stack against hydrophobic amino acids on the surface ...
... acid; F, phenylalanine; W, tryptophan). This notion was supported by the crystal structure of PP1 bound to a peptide containing the sequence RRVSFA (S, serine; A, alanine) (Egloff et al., 1997). The valine and phenylalanine residues of the peptide stack against hydrophobic amino acids on the surface ...
Evolution - Amino Acid wksht
... Proteins are made of amino acids. DNA determines what sequence these amino acids must be in to build the protein. Scientists can examine the amino acid sequence of particular protein molecules found in vertebrates to determine the degree of similarity between species. Even organisms that appear to h ...
... Proteins are made of amino acids. DNA determines what sequence these amino acids must be in to build the protein. Scientists can examine the amino acid sequence of particular protein molecules found in vertebrates to determine the degree of similarity between species. Even organisms that appear to h ...
Nucleus-Encoded Genes for Plastid
... Identification and characterization of putative plastid-targeted proteins. Genes encoding putative plastid-targeted proteins were identified from a pool of cDNA sequences produced from an ongoing Helicosporidium sp. (strain AT2000, originally isolated from the black fly, Simulium jonesii) EST projec ...
... Identification and characterization of putative plastid-targeted proteins. Genes encoding putative plastid-targeted proteins were identified from a pool of cDNA sequences produced from an ongoing Helicosporidium sp. (strain AT2000, originally isolated from the black fly, Simulium jonesii) EST projec ...
Theory and practice of size exclusion chromatography for
... purification, formulation and manufacturing of therapeutic protein products [37–40]. The kinetics or rates of aggregate formation are of primary interest because they determine the aggregate levels over a given period of time in different physical environments and at different protein concentrations ...
... purification, formulation and manufacturing of therapeutic protein products [37–40]. The kinetics or rates of aggregate formation are of primary interest because they determine the aggregate levels over a given period of time in different physical environments and at different protein concentrations ...
Translation of Cyclin mRNA Is Necessary for Extracts of Activated
... study cell-free extracts that require protein synthesis to enter mitosis and permit more than one round of DNA replication (Lohka and Masui, 1983, 1984; Hutchison et al., 1987; Blow and Laskey, 1988). If it could be shown that cyclin synthesis is necessary and sufficient to promote mitosis in vitro, ...
... study cell-free extracts that require protein synthesis to enter mitosis and permit more than one round of DNA replication (Lohka and Masui, 1983, 1984; Hutchison et al., 1987; Blow and Laskey, 1988). If it could be shown that cyclin synthesis is necessary and sufficient to promote mitosis in vitro, ...
From Amino Acid to Glucosinolate Biosynthesis: Protein Sequence
... indole instead of Trp. TSA itself has only indole-3-glycerolphosphate lyase activity and will not add Ser to the indole reaction intermediate necessary for Trp formation (Frey et al., 1997, 2000; Gierl and Frey, 2001). In these three cases, the strongest evidence for enzyme recruitment comes from ph ...
... indole instead of Trp. TSA itself has only indole-3-glycerolphosphate lyase activity and will not add Ser to the indole reaction intermediate necessary for Trp formation (Frey et al., 1997, 2000; Gierl and Frey, 2001). In these three cases, the strongest evidence for enzyme recruitment comes from ph ...
The Three-dimensional Structure of 4-Hydroxybenzoyl
... Presently, little is known concerning the evolutionary history of these 4-chlorobenzoate degrading enzymes. The exposure of bacteria to such compounds could have first occurred with the introduction of synthetic chlorinated aromatics into the environment and thus, the acquisition of the 4-chlorobenz ...
... Presently, little is known concerning the evolutionary history of these 4-chlorobenzoate degrading enzymes. The exposure of bacteria to such compounds could have first occurred with the introduction of synthetic chlorinated aromatics into the environment and thus, the acquisition of the 4-chlorobenz ...
Differential display proteomic analysis of Picea meyeri pollen
... can respond to inhibitor treatments with changes in the expression of a large number of genes that are subjected to either up- or downregulation (Fernando et al., 2001; Hao et al., 2005). Proteomics provides a deeper exploration of ...
... can respond to inhibitor treatments with changes in the expression of a large number of genes that are subjected to either up- or downregulation (Fernando et al., 2001; Hao et al., 2005). Proteomics provides a deeper exploration of ...
CD-HIT User`s Guide - Bioinformatics.org
... number of common short words is much higher than in the worst case scenarios. We did a large-‐scale statistical analysis on short words. We found, for example, even at 70% identit ...
... number of common short words is much higher than in the worst case scenarios. We did a large-‐scale statistical analysis on short words. We found, for example, even at 70% identit ...
exon junctions of Euglena gene(s) - DigitalCommons@University of
... containing 7.4 kb of the 3' end of a LHCPII gene (4). Nine exons were identified in this clone based on sequence homology with Arabidopsis LHCPII. They can be assembled into a continuous open reading frame encoding 113 amino acids of the C-terminus of an LHCPII, followed by 4 complete LHCPIIs (4). I ...
... containing 7.4 kb of the 3' end of a LHCPII gene (4). Nine exons were identified in this clone based on sequence homology with Arabidopsis LHCPII. They can be assembled into a continuous open reading frame encoding 113 amino acids of the C-terminus of an LHCPII, followed by 4 complete LHCPIIs (4). I ...
A model for mis-sense error in protein synthesis: mis
... of aa-tRNA synthetase, a mis-charged tRNA may be produced [8, 10]. Therefore, even when it turns out to be a cognate tRNA for a given codon, such a mis-charged tRNA compromises the translational fidelity by contributing an amino acid which is different from that dictated by the mRNA template. Errone ...
... of aa-tRNA synthetase, a mis-charged tRNA may be produced [8, 10]. Therefore, even when it turns out to be a cognate tRNA for a given codon, such a mis-charged tRNA compromises the translational fidelity by contributing an amino acid which is different from that dictated by the mRNA template. Errone ...