The DNA Binding Properties of Saccharomyces cerevisiae Rad51
... acetate buffer at pH values higher than 7.0, containing 10 mM magnesium acetate but without ATP, inactivates the protein with regard to DNA binding (Fig. 2). The subsequent addition of ATP to such inactivated protein failed to restore binding activity to Rad51 protein. However, the prior presence of ...
... acetate buffer at pH values higher than 7.0, containing 10 mM magnesium acetate but without ATP, inactivates the protein with regard to DNA binding (Fig. 2). The subsequent addition of ATP to such inactivated protein failed to restore binding activity to Rad51 protein. However, the prior presence of ...
Defining the complementarities between antibodies and haptens to
... recognize haptens, is still limited compared to other antigen classes. Methods: Different hapten targets with a broad range of structural flexibility and polarity were conjugated to carrier proteins, and utilized in sheep immunization. Three antibody libraries were constructed and used as potential ...
... recognize haptens, is still limited compared to other antigen classes. Methods: Different hapten targets with a broad range of structural flexibility and polarity were conjugated to carrier proteins, and utilized in sheep immunization. Three antibody libraries were constructed and used as potential ...
Substrate specificity of the periplasmic dipeptide
... traffic ATPase came from identification of a periplasmic dipeptide-binding protein (DppA) (Abouhamad et al., 1991 ; Olson et al., 1991), and subsequently from sequencing the dpp locus in E. coli, which comprises an operon of five genes (Abouhamad & Manson, 1994). Dpp has also been implicated in dipe ...
... traffic ATPase came from identification of a periplasmic dipeptide-binding protein (DppA) (Abouhamad et al., 1991 ; Olson et al., 1991), and subsequently from sequencing the dpp locus in E. coli, which comprises an operon of five genes (Abouhamad & Manson, 1994). Dpp has also been implicated in dipe ...
Biochemistry 499
... Abnormal phosphorylation has been known as a cause or consequence of many human diseases, such as cancer. This stresses the importance of regulation of the enzymes involved in reversible phosphorylation, especially the protein phosphatases. There are relatively few Ser/Thr phosphatases in the cell a ...
... Abnormal phosphorylation has been known as a cause or consequence of many human diseases, such as cancer. This stresses the importance of regulation of the enzymes involved in reversible phosphorylation, especially the protein phosphatases. There are relatively few Ser/Thr phosphatases in the cell a ...
Molecular cloning, characterization and gene expression of an
... [39]. Antioxidant related enzymes, including catalase, are known to be involved in crustaceans’ innate immune reaction [40e43]. It is reported [41,44] that white spot syndrome virus (WSSV) infection decreased the activity of antioxidant enzymes including catalase in Fenneropenaeus indicus and also [ ...
... [39]. Antioxidant related enzymes, including catalase, are known to be involved in crustaceans’ innate immune reaction [40e43]. It is reported [41,44] that white spot syndrome virus (WSSV) infection decreased the activity of antioxidant enzymes including catalase in Fenneropenaeus indicus and also [ ...
Current understanding of fatty acid biosynthesis and the acyl carrier
... system also provides acyl chains for a host of other processes. ACP (acyl carrier protein), the central cofactor protein for FA synthesis, supplies acyl chains for lipid A and lipoic acid synthesis, as well as quorum sensing, bioluminescence and toxin activation [14–18]. Furthermore, ACPs or PCPs (p ...
... system also provides acyl chains for a host of other processes. ACP (acyl carrier protein), the central cofactor protein for FA synthesis, supplies acyl chains for lipid A and lipoic acid synthesis, as well as quorum sensing, bioluminescence and toxin activation [14–18]. Furthermore, ACPs or PCPs (p ...
Selected Reaction Monitoring (SRM) to determine protein
... In reverse genetic knockout (KO) studies that aim to assign function to specific genes, confirming the reduction in abundance of the encoded protein will often aid the link between genotype and phenotype. However, measuring specific protein abundance is particularly difficult in plant research where ...
... In reverse genetic knockout (KO) studies that aim to assign function to specific genes, confirming the reduction in abundance of the encoded protein will often aid the link between genotype and phenotype. However, measuring specific protein abundance is particularly difficult in plant research where ...
The Enolase Superfamily: A General Strategy for Enzyme
... enzyme alone. In particular, those features that are important for shared chemical steps can be distinguished from those that relate to the specific chemistry of an individual enzyme. This information can then be used to predict the functions of homologs of unknown function from their sequences alon ...
... enzyme alone. In particular, those features that are important for shared chemical steps can be distinguished from those that relate to the specific chemistry of an individual enzyme. This information can then be used to predict the functions of homologs of unknown function from their sequences alon ...
PDF - Oxford Academic - Oxford University Press
... the seeds for the BLAST (18) search. RF-like proteins (1334) were found in the current protein sequence databases (GenBank translations, PIR and others). We used the ClustalX software (ftp://ftp-igbmc.u-strasbg.fr/pub/ClustalX/) (19) to align all these sequences. A maximum-likelihood species tree wa ...
... the seeds for the BLAST (18) search. RF-like proteins (1334) were found in the current protein sequence databases (GenBank translations, PIR and others). We used the ClustalX software (ftp://ftp-igbmc.u-strasbg.fr/pub/ClustalX/) (19) to align all these sequences. A maximum-likelihood species tree wa ...
Tetrahymena Contain Two Distinct and Unusual High Mobility Group
... suggesting that both of these proteins play a role in determining a macronuclear phenotype. However, a specific induction of LG-2 synthesis is detected in early stages of conjugation (meiotic prophase, 1-4 h), leading to maximal synthesis of LG-2 at 3 h. Interestingly, the early induction of LG-2 sy ...
... suggesting that both of these proteins play a role in determining a macronuclear phenotype. However, a specific induction of LG-2 synthesis is detected in early stages of conjugation (meiotic prophase, 1-4 h), leading to maximal synthesis of LG-2 at 3 h. Interestingly, the early induction of LG-2 sy ...
Herculex® I Product Overview
... The insecticidal activities of many Bt and Cry proteins have been studied in great detail and the majority are very specific, affecting only certain insects. Studies of many Bt bacterial strains, as well as their Bt proteins, have shown them to be harmless to other animals and humans. In fact, numer ...
... The insecticidal activities of many Bt and Cry proteins have been studied in great detail and the majority are very specific, affecting only certain insects. Studies of many Bt bacterial strains, as well as their Bt proteins, have shown them to be harmless to other animals and humans. In fact, numer ...
Protein relative abundance patterns associated with sucrose
... to catalog the functional potential of all microbial genes within a smaller subset of subjects. One of the key findings was that healthy sites from different people were broadly similar with respect to their functional profiles, even though there was extensive individual variation in their taxonomic ...
... to catalog the functional potential of all microbial genes within a smaller subset of subjects. One of the key findings was that healthy sites from different people were broadly similar with respect to their functional profiles, even though there was extensive individual variation in their taxonomic ...
MODified™ Protein Domain Binding Kit Manual
... (PTMs) on histone tails is generated, interpreted and edited by proteins that are coined ‘writers’ ‘readers’ and ‘erasers’. There are several classes of protein domains that influence gene regulation and chromatin remodeling by interacting with specific histone PTMs. Some common chromatin remodeling ...
... (PTMs) on histone tails is generated, interpreted and edited by proteins that are coined ‘writers’ ‘readers’ and ‘erasers’. There are several classes of protein domains that influence gene regulation and chromatin remodeling by interacting with specific histone PTMs. Some common chromatin remodeling ...
Johansson L, Gafvelin G, Arnér ES. Selenocysteine in proteins
... than Cys. Proteins naturally containing Sec are often enzymes, employing the reactivity of the Sec residue during the catalytic cycle and therefore Sec is normally essential for their catalytic efficiencies. Other unique features of Sec, not shared by any of the other 20 common amino acids, derive f ...
... than Cys. Proteins naturally containing Sec are often enzymes, employing the reactivity of the Sec residue during the catalytic cycle and therefore Sec is normally essential for their catalytic efficiencies. Other unique features of Sec, not shared by any of the other 20 common amino acids, derive f ...
Nitrate reductase activity in chicory roots
... plant with reduced nitrogen (Dorchies and Rambour, 1985). Other enzymatic activities involved in the nitrate assimilation pathway such as glutamate synthase or glutamine synthetase show similar seasonal modifications (Sechley et al., 1991). At that later stage, roots accumulate inulin and reduced ni ...
... plant with reduced nitrogen (Dorchies and Rambour, 1985). Other enzymatic activities involved in the nitrate assimilation pathway such as glutamate synthase or glutamine synthetase show similar seasonal modifications (Sechley et al., 1991). At that later stage, roots accumulate inulin and reduced ni ...
Altered pathogenicity, immunogenicity, tissue tropism and 3`
... attachment to cells. Furthermore, it has been shown that S1 is a major determinant of cell tropism in culture ...
... attachment to cells. Furthermore, it has been shown that S1 is a major determinant of cell tropism in culture ...
The use of deuteration for the structural study of larger proteins
... • deuteration level of 50–60% → one sample for backbone and sidechain assignment • 50–60% D is also a reasonable compromise to get NOE information • various isotopomers contribute similarly to diffferent experiments → less problems with isotope shifts • clearly not good enough for proteins > 35 kDa ...
... • deuteration level of 50–60% → one sample for backbone and sidechain assignment • 50–60% D is also a reasonable compromise to get NOE information • various isotopomers contribute similarly to diffferent experiments → less problems with isotope shifts • clearly not good enough for proteins > 35 kDa ...
9. proteins i
... intimately connected with all phases of chemical and physical activity, that constitute the life of the cell. Some proteins serve as important structural elements of the body, for example, as hair, wool and collagen, an important constituent of connective tissue ; other proteins may be enzymes, horm ...
... intimately connected with all phases of chemical and physical activity, that constitute the life of the cell. Some proteins serve as important structural elements of the body, for example, as hair, wool and collagen, an important constituent of connective tissue ; other proteins may be enzymes, horm ...
ref. #28 of the TIBS article
... a model transmembrane helix in the endoplasmic reticulum membrane using the glycosylation mapping technique. Asp and Glu residues cause a re-positioning of the C-terminal end of the transmembrane helix when placed in the one to two C-terminal turns but not when placed more centrally. Arg and Lys res ...
... a model transmembrane helix in the endoplasmic reticulum membrane using the glycosylation mapping technique. Asp and Glu residues cause a re-positioning of the C-terminal end of the transmembrane helix when placed in the one to two C-terminal turns but not when placed more centrally. Arg and Lys res ...
Green Fluorescent Protein
... • One limitation of wtGFP is its slow rate of fluorescence acquisition in vivo • Renaturation most likely by a parallel pathway • Oxidation of Fluoropore (2-4 hours) • Two step process ...
... • One limitation of wtGFP is its slow rate of fluorescence acquisition in vivo • Renaturation most likely by a parallel pathway • Oxidation of Fluoropore (2-4 hours) • Two step process ...
An introduction to informatics - Swiss
... Dear Sir/Madame, I have a question concerning selection of data from UniProt protein database. I wonder if there are any examples of two or more protein entries, which concern exactly the same protein of two or more individuals representing the same species. In other words, I would like to know, if ...
... Dear Sir/Madame, I have a question concerning selection of data from UniProt protein database. I wonder if there are any examples of two or more protein entries, which concern exactly the same protein of two or more individuals representing the same species. In other words, I would like to know, if ...
Natural selection shaped regional mtDNA
... version 10.0, GCG). Coalescence Dates for Haplogroups. The times to the most recent common ancestor (MRCA) were calculated by using only the mtDNA coding region (nucleotide positions 577–16023), because of the high probability of reverse mutations in the control region. The average sequence evolutio ...
... version 10.0, GCG). Coalescence Dates for Haplogroups. The times to the most recent common ancestor (MRCA) were calculated by using only the mtDNA coding region (nucleotide positions 577–16023), because of the high probability of reverse mutations in the control region. The average sequence evolutio ...
List of abbreviations
... period of 48 hours, not having been present at the time of patient hospitalization admission, or within 48 hours after discharge, and the result of medical intervention [1,2]. Nowadays, HAIs are, by far, one of the most common complications among hospitalized patients. The World Health Organization ...
... period of 48 hours, not having been present at the time of patient hospitalization admission, or within 48 hours after discharge, and the result of medical intervention [1,2]. Nowadays, HAIs are, by far, one of the most common complications among hospitalized patients. The World Health Organization ...
LNBI 9043 - Lupin Allergy: Uncovering Structural Features and
... big conformational epitopicc area (black color, Fig. 3) and 2 lineal epitopes indepeendent. Furthermore, β 2 and β 5 present 3 conformational epitopic areas, 1 in β 3, β 6 and β 7, 2 in β 4, related to the differential mobile arm structure. The biggest difference as structural feature between the β ...
... big conformational epitopicc area (black color, Fig. 3) and 2 lineal epitopes indepeendent. Furthermore, β 2 and β 5 present 3 conformational epitopic areas, 1 in β 3, β 6 and β 7, 2 in β 4, related to the differential mobile arm structure. The biggest difference as structural feature between the β ...
Combining docking and molecular dynamic simulations in drug design
... The development of new drugs is undoubtedly one of the most challenging tasks of today’s science. Driven by the combined efforts of the pharmaceutical industry, biotech companies, regulatory authorities, academic researchers, and other private and public sectors, the development of new drugs is a ve ...
... The development of new drugs is undoubtedly one of the most challenging tasks of today’s science. Driven by the combined efforts of the pharmaceutical industry, biotech companies, regulatory authorities, academic researchers, and other private and public sectors, the development of new drugs is a ve ...