Improving muscle mass: response of muscle metabolism to exercise
... in positive NBAL. Thus muscle protein is alternately deposited and released depending on the metabolic situation. The magnitude and duration of these periods of muscle protein deposition and release determine the amount of muscle mass (Figure 1). In healthy, weight-stable adults, the periods of posi ...
... in positive NBAL. Thus muscle protein is alternately deposited and released depending on the metabolic situation. The magnitude and duration of these periods of muscle protein deposition and release determine the amount of muscle mass (Figure 1). In healthy, weight-stable adults, the periods of posi ...
Disallowed Ramachandran Conformations of Amino Acid Residues
... Is the unusual stereochemistry observed at the disallowed residues an intrinsic feature dictated by the structural environment or is it a lingering artifact of the structure determination process, despite a rigorous attempt to screen the data base? (Kleywegt & Jones, 1995). The availability of struc ...
... Is the unusual stereochemistry observed at the disallowed residues an intrinsic feature dictated by the structural environment or is it a lingering artifact of the structure determination process, despite a rigorous attempt to screen the data base? (Kleywegt & Jones, 1995). The availability of struc ...
Proteins and their Ligands: Their Importance and how to Crystallize
... The importance of structural biology has been highlighted in the past few years not only as part of drug discovery programs in the pharmaceutical industry but also by structural genomics programs. Although the function of a protein can be studied by several biochemical and or biophysical techniques ...
... The importance of structural biology has been highlighted in the past few years not only as part of drug discovery programs in the pharmaceutical industry but also by structural genomics programs. Although the function of a protein can be studied by several biochemical and or biophysical techniques ...
A wide-range phylogenetic analysis of Zic proteins: Implications for
... process of Zic genes as follows (Fig. 5A). The earliest Zic genes that appeared in ancestral Eumetazoa or metazoans, probably in the pre-Cambrian era, may have shared common structural features, namely the ZF and ZF-NC domains. Exon–intron boundaries are not conserved between Eumetazoa and cnidarian ...
... process of Zic genes as follows (Fig. 5A). The earliest Zic genes that appeared in ancestral Eumetazoa or metazoans, probably in the pre-Cambrian era, may have shared common structural features, namely the ZF and ZF-NC domains. Exon–intron boundaries are not conserved between Eumetazoa and cnidarian ...
Sample Chapter 5: Amino Acids, Peptides, and Proteins
... to perform its functions is specified by its unique amino acid sequence. During synthesis each polypeptide molecule bends in three-dimensional space as its amino acid components (called amino acid residues) interact with each other, largely through noncovalent interactions. The subsequent folding of ...
... to perform its functions is specified by its unique amino acid sequence. During synthesis each polypeptide molecule bends in three-dimensional space as its amino acid components (called amino acid residues) interact with each other, largely through noncovalent interactions. The subsequent folding of ...
Turnover-based in vitro selection and evolution of biocatalysts from
... (b) DFMPP, immobilized to BSA or transferrin, was used in turnover selection from the phage-displayed HuCAL-scFv for catalysis of phosphate monoester hydrolysis. This selection approach covers the entire cycle of phosphate monoester hydrolysis and subsequently leads to formation of an electrophilic ...
... (b) DFMPP, immobilized to BSA or transferrin, was used in turnover selection from the phage-displayed HuCAL-scFv for catalysis of phosphate monoester hydrolysis. This selection approach covers the entire cycle of phosphate monoester hydrolysis and subsequently leads to formation of an electrophilic ...
Case Study 5 Literature - Department of Chemistry
... amino acid residues with a calculated molecular mass of 55 kDa. The deduced amino acid sequence corresponds with the partial amino acid sequences of the three CNBr fragments prepared from the protein and a tryptic peptide published earlier (8), thus identifying the open reading frame as the coding g ...
... amino acid residues with a calculated molecular mass of 55 kDa. The deduced amino acid sequence corresponds with the partial amino acid sequences of the three CNBr fragments prepared from the protein and a tryptic peptide published earlier (8), thus identifying the open reading frame as the coding g ...
Plasmodium falciparum enolase - Tata Institute of Fundamental
... and some different residues [17]. These include Cys157. The analogous residue in Trypanosoma brucei enolase (Cys147) has recently been shown to be modified with iodoacetamide [18,19]. Reaction with iodoacetamide also leads to partial inactivation of the enzyme. It will be interesting to examine wheth ...
... and some different residues [17]. These include Cys157. The analogous residue in Trypanosoma brucei enolase (Cys147) has recently been shown to be modified with iodoacetamide [18,19]. Reaction with iodoacetamide also leads to partial inactivation of the enzyme. It will be interesting to examine wheth ...
Formate Dehydrogenase, an Enzyme of Anaerobic Metabolism, Is
... Chemical or enzymatic digestion was used to determine the internal sequence of the proteins. Chemical digestion with CNBr was according to the method of Gross (1967) with the following modifications. Isolated protein spots from 50 2D PAGE gels were pooled by electroblotting onto a PVDF membrane acco ...
... Chemical or enzymatic digestion was used to determine the internal sequence of the proteins. Chemical digestion with CNBr was according to the method of Gross (1967) with the following modifications. Isolated protein spots from 50 2D PAGE gels were pooled by electroblotting onto a PVDF membrane acco ...
Distribution and phylogenies of enzymes of the Embden
... ecosystems have been suggested to represent the most ancient, continuously inhabited microbial habitats on Earth (Reysenbach and Shock 2002). In addition, geological evidence supports the view that hot aquatic habitats existed throughout the early history of Earth, regardless of the temperature of t ...
... ecosystems have been suggested to represent the most ancient, continuously inhabited microbial habitats on Earth (Reysenbach and Shock 2002). In addition, geological evidence supports the view that hot aquatic habitats existed throughout the early history of Earth, regardless of the temperature of t ...
MS#5_(Cueno and Laude).indd - Philippine Journal of Science
... (Banzon and Velasco 1982; Harries 1994) further suggesting ontogenetic gene expression which coincides with the hypothesis made by Villalobos et al. (2001). This would suggest a temporal pattern of gene regulation among coconut genes involved in fatty acid synthesis. The untranslated region (UTR) is ...
... (Banzon and Velasco 1982; Harries 1994) further suggesting ontogenetic gene expression which coincides with the hypothesis made by Villalobos et al. (2001). This would suggest a temporal pattern of gene regulation among coconut genes involved in fatty acid synthesis. The untranslated region (UTR) is ...
Membrane Topology of Cytochrome P450 2B4 in
... with a single transmembrane α-helical anchor, exhibits identical biphasic kinetics when injected beneath phosphatidylcholine monolayers. The soluble cytochromes, P450cam and Mb, and the truncated proteins, P450 2B4 Δ2–27 and Δb5, in contrast, induce only slow-phase pressure changes. The lack of a fa ...
... with a single transmembrane α-helical anchor, exhibits identical biphasic kinetics when injected beneath phosphatidylcholine monolayers. The soluble cytochromes, P450cam and Mb, and the truncated proteins, P450 2B4 Δ2–27 and Δb5, in contrast, induce only slow-phase pressure changes. The lack of a fa ...
Journal of Biological Chemistry
... upon amino acid incorporation into protein of the liver ribosome system (Table II) just as effectively as it does the decrease in hepatic ATP concentration induced by the same analogue (3). These results tend to implicate cellular BTP deficiency as being important in the inhibition of protein synthe ...
... upon amino acid incorporation into protein of the liver ribosome system (Table II) just as effectively as it does the decrease in hepatic ATP concentration induced by the same analogue (3). These results tend to implicate cellular BTP deficiency as being important in the inhibition of protein synthe ...
Characterisation of Milk Proteins in Confectionery Products
... although representing only a small percentage (3-4 %) of its total composition are considered to possess unique structural and functional properties which will influence these final quality parameters as well as affecting processing. Of particular impor tance in confectionery are organoleptic, hydra ...
... although representing only a small percentage (3-4 %) of its total composition are considered to possess unique structural and functional properties which will influence these final quality parameters as well as affecting processing. Of particular impor tance in confectionery are organoleptic, hydra ...
Cloning and functional expression of invertebrate connexins from
... sequences [8]. However, this cursory analysis did not show any data on sequence alignments, provide any figures of molecular phylogeny, discuss gene structure, or most importantly, demonstrate that the connexin-like sequences encoded proteins that actually functioned as gap junction channels. Thus, w ...
... sequences [8]. However, this cursory analysis did not show any data on sequence alignments, provide any figures of molecular phylogeny, discuss gene structure, or most importantly, demonstrate that the connexin-like sequences encoded proteins that actually functioned as gap junction channels. Thus, w ...
Ube2W conjugates ubiquitin to α-amino groups of protein N
... Ubiquitin is conjugated to target proteins in a three-step reaction where the ubiquitin is first adenylated, then covalently linked via a thioester bond to a cysteine residue in an E1 enzyme (ubiquitin-activating enzyme). The ubiquitin is then transferred via a second thioester bond on to an E2 enzy ...
... Ubiquitin is conjugated to target proteins in a three-step reaction where the ubiquitin is first adenylated, then covalently linked via a thioester bond to a cysteine residue in an E1 enzyme (ubiquitin-activating enzyme). The ubiquitin is then transferred via a second thioester bond on to an E2 enzy ...
as a PDF
... PHF-T (Greenberg and Davies, 1990; Ksiezak-Reding and Yen, 1991; Lee et al., 1991), suggesting a possible involvement of kinase FA/GSK-3a in the abnormal phosphorylation of pathological PHF-T in Alzheimer's disease brain (Yang et al., 1991a, 1993b) . The phosphorylation of T by protein kinase FA/GSK ...
... PHF-T (Greenberg and Davies, 1990; Ksiezak-Reding and Yen, 1991; Lee et al., 1991), suggesting a possible involvement of kinase FA/GSK-3a in the abnormal phosphorylation of pathological PHF-T in Alzheimer's disease brain (Yang et al., 1991a, 1993b) . The phosphorylation of T by protein kinase FA/GSK ...
Amino Acid Sequences and Evolutionary Relationships - Parkway C-2
... An important technique used in determining evolutionary relationships is the biochemical comparison of organisms. Organisms that are not similar in physical appearance may show similarity in amino acid sequences for homologous proteins. Two proteins are commonly studied by scientists who are attempt ...
... An important technique used in determining evolutionary relationships is the biochemical comparison of organisms. Organisms that are not similar in physical appearance may show similarity in amino acid sequences for homologous proteins. Two proteins are commonly studied by scientists who are attempt ...
View/Open - VUW research archive - Victoria University of Wellington
... Bede Busby, Peter Birchirm, Katie Zeier, and Dr Christine Stockholm. Thank you for sharing your time and friendship. Lastly, to my family, you have been such a great support group. Thank you all so much!!! ...
... Bede Busby, Peter Birchirm, Katie Zeier, and Dr Christine Stockholm. Thank you for sharing your time and friendship. Lastly, to my family, you have been such a great support group. Thank you all so much!!! ...
Full-Text PDF
... Pollen from different species of monocotyledonic or and dicotyledonic plants is regarded to be the most frequent and active allergen source. Pollen allergens are low molecular weight proteins or glycoproteins and are located intracellular in the pollen grains. Their physiological activation starts a ...
... Pollen from different species of monocotyledonic or and dicotyledonic plants is regarded to be the most frequent and active allergen source. Pollen allergens are low molecular weight proteins or glycoproteins and are located intracellular in the pollen grains. Their physiological activation starts a ...
Green Fluorescent Protein
... GFP as a biosensor GFP is used within various fields of biology like molecular biology, neuroscience and cell biology. It can be used as a reporter gene, cell marker, fusion tag, and for quantitative monitoring of gene expression. GFP’s fluorescence characteristic can also be used in methods design ...
... GFP as a biosensor GFP is used within various fields of biology like molecular biology, neuroscience and cell biology. It can be used as a reporter gene, cell marker, fusion tag, and for quantitative monitoring of gene expression. GFP’s fluorescence characteristic can also be used in methods design ...
The Serum Proteins of the Rat During Development
... An asymmetry was found in the trailing region of the gamma-globulin fraction during all stages of development, including the adult. An asymmetry very similar in appearance and position was also found by Gurvich & Karsaevskaya (1956), but only in sera from perinatal animals. These workers have design ...
... An asymmetry was found in the trailing region of the gamma-globulin fraction during all stages of development, including the adult. An asymmetry very similar in appearance and position was also found by Gurvich & Karsaevskaya (1956), but only in sera from perinatal animals. These workers have design ...
Template-Synthesized Protein Nanotubes
... is immersed first into a solution of 3-amino propylphosphonic acid (APA), resulting in attachment (again via the phosphonate) of a monolayer of this molecule to the pore walls. The amino groups are then reacted with an excess quantity of the protein-immobilization agent glutaraldehyde (GA),14,15 lea ...
... is immersed first into a solution of 3-amino propylphosphonic acid (APA), resulting in attachment (again via the phosphonate) of a monolayer of this molecule to the pore walls. The amino groups are then reacted with an excess quantity of the protein-immobilization agent glutaraldehyde (GA),14,15 lea ...
STRUCTURAL AND FUNCTIONAL STUDIES OF PYRIDOXINE 5’-PHOSPHATE SYNTHASE E. COLI Doctoral Thesis
... acids. Whereas microorganisms and plants can synthetise vitamin B6 de novo, mammals have to obtain one of the B6 vitamers with their diet. The Escherichia coli biosynthetic machinery is the, by far, best characterised and it consists in four pdx proteins. PdxJ, also referred to as pyridoxine 5’-phos ...
... acids. Whereas microorganisms and plants can synthetise vitamin B6 de novo, mammals have to obtain one of the B6 vitamers with their diet. The Escherichia coli biosynthetic machinery is the, by far, best characterised and it consists in four pdx proteins. PdxJ, also referred to as pyridoxine 5’-phos ...
Porino Va - UROP
... Beta peptide dimers with biaryl cross-links have the potential to disrupt protein-protein interactions. Binding to one outside edge of a beta peptide dimer should cooperatively enhance binding to the opposite outside edge by the transmission of polarization between them. Dimers can be made to target ...
... Beta peptide dimers with biaryl cross-links have the potential to disrupt protein-protein interactions. Binding to one outside edge of a beta peptide dimer should cooperatively enhance binding to the opposite outside edge by the transmission of polarization between them. Dimers can be made to target ...