Proteolytic processing in the secretory pathway of Aspergillus niger
... proteases. A brief explanation of these four groups is given here; a more elaborate description is given by van den Hombergh (1996). Each group has a different proteolytic mechanism and therefore different enzymatic characteristics and inhibitors. The cysteine proteases are a rare group of proteases ...
... proteases. A brief explanation of these four groups is given here; a more elaborate description is given by van den Hombergh (1996). Each group has a different proteolytic mechanism and therefore different enzymatic characteristics and inhibitors. The cysteine proteases are a rare group of proteases ...
SPECIFIC PROTEIN SYNTHESIS IN CELLULAR
... synthesis was stopped rapidly by transferring the tissue to 2-4 ml of an ice slurry of hypotonie buffer containing 10 mm Tris-HCI, pH 7.4, 50 mm KCI, and 10 mm MgC1 2 at 0 °C in a glass 7 ml Dounce homogenizer. The KCI concentration was changed where noted in the Results . All the following operatio ...
... synthesis was stopped rapidly by transferring the tissue to 2-4 ml of an ice slurry of hypotonie buffer containing 10 mm Tris-HCI, pH 7.4, 50 mm KCI, and 10 mm MgC1 2 at 0 °C in a glass 7 ml Dounce homogenizer. The KCI concentration was changed where noted in the Results . All the following operatio ...
Document
... NMR-visible component display characteristic chemical shift changes upon increasing amounts of the additionally expressed, unlabeled ligand [24,25]. This effect can be employed to comparatively analyze in vitro and in vivo binding behaviors of known interaction partners, or to test how small molec ...
... NMR-visible component display characteristic chemical shift changes upon increasing amounts of the additionally expressed, unlabeled ligand [24,25]. This effect can be employed to comparatively analyze in vitro and in vivo binding behaviors of known interaction partners, or to test how small molec ...
Predictable Alteration of Sequence Recognition by RNA
... RNA editing factors of the pentatricopeptide repeat (PPR) family show a very high degree of sequence specificity in the recognition of their target sites. A molecular basis for target recognition by editing factors has been proposed based on statistical correlations but has not been tested experiment ...
... RNA editing factors of the pentatricopeptide repeat (PPR) family show a very high degree of sequence specificity in the recognition of their target sites. A molecular basis for target recognition by editing factors has been proposed based on statistical correlations but has not been tested experiment ...
Whey Products - New Zealand Institute of Chemistry
... Whey Protein Concentrate (WPC) is produced using ultrafiltration. This is also a membrane separation, but it selects on the basis of molecular size and is driven by pressure rather than by applied electric field as in the case of electrodialysis. Ultrafiltration retains (in the liquid product termed ...
... Whey Protein Concentrate (WPC) is produced using ultrafiltration. This is also a membrane separation, but it selects on the basis of molecular size and is driven by pressure rather than by applied electric field as in the case of electrodialysis. Ultrafiltration retains (in the liquid product termed ...
Topology Prediction of Membrane Proteins
... composed of mainly hydrophobic residues where the side chains can form van der Waals interactions with the fatty acid chains in the membrane core. All polar amide and carbonyl groups in the backbone are hydrogen bonded internally within the helix. This lowers the cost of transferring polar entities ...
... composed of mainly hydrophobic residues where the side chains can form van der Waals interactions with the fatty acid chains in the membrane core. All polar amide and carbonyl groups in the backbone are hydrogen bonded internally within the helix. This lowers the cost of transferring polar entities ...
Identification of a Chloroplast-encoded 9-kDa
... haverevealed the involvement of at least five different electron acceptors denoted A,, AI, X, B, and A (1-3). Whereas spectrophotophysical techniques have been very useful in detecting these acceptors in complex preparations, the same techniques are of limited value in determining the identity of th ...
... haverevealed the involvement of at least five different electron acceptors denoted A,, AI, X, B, and A (1-3). Whereas spectrophotophysical techniques have been very useful in detecting these acceptors in complex preparations, the same techniques are of limited value in determining the identity of th ...
PowerPoint 演示文稿
... mRNA has the same function in all cells, but there are important differences in the details of the synthesis and structure between prokaryotic and eukaryotic mRNA. ...
... mRNA has the same function in all cells, but there are important differences in the details of the synthesis and structure between prokaryotic and eukaryotic mRNA. ...
Molecular evolutionary analysis of the American pika
... more frequently than changes to protein structure and function (King and Jukes 1969; Kimura 1984). This is because most nucleotide and amino acid substitutions in evolution occur by mutation and random genetic drift, and the majority of molecular variation within a population is neutral (Nei 1987). ...
... more frequently than changes to protein structure and function (King and Jukes 1969; Kimura 1984). This is because most nucleotide and amino acid substitutions in evolution occur by mutation and random genetic drift, and the majority of molecular variation within a population is neutral (Nei 1987). ...
Molecular Characterisation of the 76 kDa Iron
... Genes encoding subunits of complex I (EC 1.6.5.3) of the mitochondrial respiratory chain vary in their locations between the mitochondrial and nuclear genomes in different organisms, whereas genes for a homologous multisubunit complex in chloroplasts have to date only been found on the plastid genom ...
... Genes encoding subunits of complex I (EC 1.6.5.3) of the mitochondrial respiratory chain vary in their locations between the mitochondrial and nuclear genomes in different organisms, whereas genes for a homologous multisubunit complex in chloroplasts have to date only been found on the plastid genom ...
The world of proteases Diversity and function
... Protease activity can be measured in many ways using different substrates and different reaction conditions There is not one correct way to measure protease activity An activity number is always dependent on the exact assay conditions Therefore, activity units/protease assays cannot be used to evalu ...
... Protease activity can be measured in many ways using different substrates and different reaction conditions There is not one correct way to measure protease activity An activity number is always dependent on the exact assay conditions Therefore, activity units/protease assays cannot be used to evalu ...
- Sportscience
... A considerable amount of research has evaluated dietary protein needs of athletes. Although there is some debate, most studies indicate that in order to maintain protein balance during intense resistance and/or endurance training, athletes should ingest approximately 1.3 to 1.8 g protein per kg body ...
... A considerable amount of research has evaluated dietary protein needs of athletes. Although there is some debate, most studies indicate that in order to maintain protein balance during intense resistance and/or endurance training, athletes should ingest approximately 1.3 to 1.8 g protein per kg body ...
Amino acid residues that determine functional specificity of NADP
... Apart from 344Lys, 345Tyr, and 351Val, several other amino acids contact cofactor in most considered structures: substrate-specific residues 103Leu, 105Thr, 337Ala, and 341Thr contact the nicotinamide nucleotide and thus spatially lie between the cofactor-binding and the substratebinding pockets. The ...
... Apart from 344Lys, 345Tyr, and 351Val, several other amino acids contact cofactor in most considered structures: substrate-specific residues 103Leu, 105Thr, 337Ala, and 341Thr contact the nicotinamide nucleotide and thus spatially lie between the cofactor-binding and the substratebinding pockets. The ...
Introduction to Protein Structure
... problem of the relationship between structure and function in enzymes. […] He proposed that the information determining the tertiary structure of a protein resides in the chemistry of its amino acid sequence. […] It was demonstrated that, after cleavage of disulfide bonds and disruption of tertiary ...
... problem of the relationship between structure and function in enzymes. […] He proposed that the information determining the tertiary structure of a protein resides in the chemistry of its amino acid sequence. […] It was demonstrated that, after cleavage of disulfide bonds and disruption of tertiary ...
PROTEIN SUBCELLULAR LOCALIZATION
... WoLF PSORT was designed with dual localization in mind. The only dual localization category for which SWISS-PROT currently contains a significant number of entries is dual localization to the nucleus and cytosol. This is an important category of proteins, for example some transcription factors are r ...
... WoLF PSORT was designed with dual localization in mind. The only dual localization category for which SWISS-PROT currently contains a significant number of entries is dual localization to the nucleus and cytosol. This is an important category of proteins, for example some transcription factors are r ...
Recombinant Brassinosteroid Insensitive 1 Receptor-Like
... eliminating S-1163 as a possible phosphorylation site. In all, at least 12 sites of in vitro autophosphorylation in the BRI-KD were identified, five uniquely and seven with some remaining ambiguity about the specific phosphorylated residue(s) within particular peptide fragments. To determine if the ...
... eliminating S-1163 as a possible phosphorylation site. In all, at least 12 sites of in vitro autophosphorylation in the BRI-KD were identified, five uniquely and seven with some remaining ambiguity about the specific phosphorylated residue(s) within particular peptide fragments. To determine if the ...
Affinity Chromatography
... In this seminal paper, the general principles and potential application of affinity chromatography were enunciated and have largely remained unchanged until the present date. The paper contained several important contributions. First, it generalized the technique to all potential enzyme purification ...
... In this seminal paper, the general principles and potential application of affinity chromatography were enunciated and have largely remained unchanged until the present date. The paper contained several important contributions. First, it generalized the technique to all potential enzyme purification ...
Isoelectric point prediction from the amino acid sequence of a protein
... set of proteins was collected and organized ...
... set of proteins was collected and organized ...
Comparison of Genes Encoding Enzymes of Sterol Biosynthesis
... biosynthesis has been extensively studied in the model yeast Saccharomyces cerevisiae. In this study here, by the aid of computational approach, yeast orthologs of sterol pathway genes and their genomic copy number alterations in the model magnoliophytes (Arabidopsis thaliana, Populus trichocarpa, O ...
... biosynthesis has been extensively studied in the model yeast Saccharomyces cerevisiae. In this study here, by the aid of computational approach, yeast orthologs of sterol pathway genes and their genomic copy number alterations in the model magnoliophytes (Arabidopsis thaliana, Populus trichocarpa, O ...
Cloning and Expression of Bovine Sodium/Glucose Cotransporters* J. Dairy Sci. 88:182–194
... Clontech) and SGLT-R2 resulted in 5′ sequence of bSGLT1. However, the RACE using UPM and SGLTR1 resulted in 5′ sequence of bSGLT5, presumably due to high sequence homology between the 2 transporters. The 3′ sequences of bSGLT1 and bSGLT5 were amplified using the primers UPM and SGLT-F2 designed from ...
... Clontech) and SGLT-R2 resulted in 5′ sequence of bSGLT1. However, the RACE using UPM and SGLTR1 resulted in 5′ sequence of bSGLT5, presumably due to high sequence homology between the 2 transporters. The 3′ sequences of bSGLT1 and bSGLT5 were amplified using the primers UPM and SGLT-F2 designed from ...
Biotechnology and Bioengineering
... closest C-C distance from FeA and FeB is 8.0 Å and 7.0 Å away, respectively. It is also just one amino acid away from the iron ligand Q197 that makes up the catalytic center of the enzyme (McCormick and Lippard 2011). Because of this proximity, it was suggested that altering F196 could lead to chang ...
... closest C-C distance from FeA and FeB is 8.0 Å and 7.0 Å away, respectively. It is also just one amino acid away from the iron ligand Q197 that makes up the catalytic center of the enzyme (McCormick and Lippard 2011). Because of this proximity, it was suggested that altering F196 could lead to chang ...
Nucleotide Sequence of fruA, the Gene Specifying Enzyme IIfru of
... of these derivatives were separated into membrane and cytoplasmic fractions; these could be tested for the presence of active FPr/Enzyme 111"" (cytoplasmic) and Enzyme IIfru(membrane), by measuring their ability to complement extracts of mutants impaired in specific known components of the fructose- ...
... of these derivatives were separated into membrane and cytoplasmic fractions; these could be tested for the presence of active FPr/Enzyme 111"" (cytoplasmic) and Enzyme IIfru(membrane), by measuring their ability to complement extracts of mutants impaired in specific known components of the fructose- ...
design of lupin seeds lactic acid fermentation – changes of
... per mL of the above individual LAB strains, was added. Fermentation of lupin was carried out 24 h at 30°C for L. sakei, 32°C for P. acidilactici and 35°C for P. pentosaceus. At the end of fermentation, the colony number in the fermented lupin was on the average of 7.28 log10 CFU g1, and the final m ...
... per mL of the above individual LAB strains, was added. Fermentation of lupin was carried out 24 h at 30°C for L. sakei, 32°C for P. acidilactici and 35°C for P. pentosaceus. At the end of fermentation, the colony number in the fermented lupin was on the average of 7.28 log10 CFU g1, and the final m ...
HIS-Select Nickel Affinity Gel (P6611) - Technical - Sigma
... The recombinant protein with a histidine containing tag may be extracted from a crude cell extract or a partially purified protein fraction prepared by standard techniques. The protein sample preparation steps should be empirically determined by the end user since the conditions may vary depending o ...
... The recombinant protein with a histidine containing tag may be extracted from a crude cell extract or a partially purified protein fraction prepared by standard techniques. The protein sample preparation steps should be empirically determined by the end user since the conditions may vary depending o ...
PDF - University of California, San Francisco
... Reductase-Hydrophobicity plots of the amino acid sequence were FIG. 1. Amino acid sequence and domain map of hamster generated by the method of Kyte and Doolittle (10). The hydrophoHMG-CoA reductase. Panel A , amino acid sequence. Amino acid bicity values used for each amino acid were those of Eisen ...
... Reductase-Hydrophobicity plots of the amino acid sequence were FIG. 1. Amino acid sequence and domain map of hamster generated by the method of Kyte and Doolittle (10). The hydrophoHMG-CoA reductase. Panel A , amino acid sequence. Amino acid bicity values used for each amino acid were those of Eisen ...