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... Analyzing vertebrate genomes requires rapid mRNA/DNA and crossspecies protein alignments. BLAT (the BLAST-like alignment tool) was developed by Jim Kent from UCSC. It is more accurate and 500 times faster than popular existing tools such as BLAST for mRNA/DNA alignments and 50 times faster for prote ...
... Analyzing vertebrate genomes requires rapid mRNA/DNA and crossspecies protein alignments. BLAT (the BLAST-like alignment tool) was developed by Jim Kent from UCSC. It is more accurate and 500 times faster than popular existing tools such as BLAST for mRNA/DNA alignments and 50 times faster for prote ...
Matthew Kwong - GEP Community Server
... exon). Looking at Figure 6, one can see that this exon, whose query sequence is bp 17645-17529, has a final exon peptide sequence of HKGKVHS. Looking at a close-up window of the donating site (Figure 9), one can see the peptide sequence corresponding with the final exon (blue box). This observation ...
... exon). Looking at Figure 6, one can see that this exon, whose query sequence is bp 17645-17529, has a final exon peptide sequence of HKGKVHS. Looking at a close-up window of the donating site (Figure 9), one can see the peptide sequence corresponding with the final exon (blue box). This observation ...
Microcytic Anemia Objectives
... Alpha Thalassemia Four identical genes are responsible for the production of alpha-globin, two on each copy of chromosome 16. Mutations in these genes often result in stop codons, thus knocking out a gene’s alpha-globin production entirely (ie, the gene is either normal and “on” or abnormal and “off ...
... Alpha Thalassemia Four identical genes are responsible for the production of alpha-globin, two on each copy of chromosome 16. Mutations in these genes often result in stop codons, thus knocking out a gene’s alpha-globin production entirely (ie, the gene is either normal and “on” or abnormal and “off ...
DANIELE GHEZZI Matr. N°. 708361 Identification and
... mtDNA remains true in practice for genetic counselling and for evolutionary studies. Each human cell has thousands of mitochondria and within each single mitochondrion are present multiple copies (2 to 10) of mtDNA. Usually all these copies are identical, a status known as homoplasmy. However it is ...
... mtDNA remains true in practice for genetic counselling and for evolutionary studies. Each human cell has thousands of mitochondria and within each single mitochondrion are present multiple copies (2 to 10) of mtDNA. Usually all these copies are identical, a status known as homoplasmy. However it is ...
Temporal and Spatial Expression of Homeotic Genes Is Important for
... regulates the expression of genes, such as, reverse polarity (repo) (Halter et al., 1995; Xiong et al., 1994), which encodes a homeodomain protein and is expressed exclusively in almost every developing glia with few exceptions. MM-CBG, which are a type of glia, have segmentspecificity determined by ...
... regulates the expression of genes, such as, reverse polarity (repo) (Halter et al., 1995; Xiong et al., 1994), which encodes a homeodomain protein and is expressed exclusively in almost every developing glia with few exceptions. MM-CBG, which are a type of glia, have segmentspecificity determined by ...
The wbbD gene of E. coli strain VW187
... stains of the fusion protein separated by SDS–polyacrylamide gel electrophoresis (PAGE) showed a major band at 57 kDa, suggesting a high level of expression. Surprisingly, however, the purified enzyme protein had very little activity (less than 1% of activity of the homogenate from DH5α carrying pCM ...
... stains of the fusion protein separated by SDS–polyacrylamide gel electrophoresis (PAGE) showed a major band at 57 kDa, suggesting a high level of expression. Surprisingly, however, the purified enzyme protein had very little activity (less than 1% of activity of the homogenate from DH5α carrying pCM ...
Lampbrush Chromosomes of the Chicken
... small distinct set of loops. In general, loops on this chromosome seems less extended than those on other chromosomes in the same spread. Chromosome length and loop size are a function of the stage in the progressive formation and retraction/compaction process as diplotene progresses and the oocyte ...
... small distinct set of loops. In general, loops on this chromosome seems less extended than those on other chromosomes in the same spread. Chromosome length and loop size are a function of the stage in the progressive formation and retraction/compaction process as diplotene progresses and the oocyte ...
A Resurrection of B Chromosomes?
... Although plant centromeres can be very large (Jin et al., 2004), more recent data suggest that the size of a functional centromere might be only a few hundred kilobases (Nagaki et al., 2004). It has been shown that barley centromeric repeats are neither necessary nor sufficient to establish a centro ...
... Although plant centromeres can be very large (Jin et al., 2004), more recent data suggest that the size of a functional centromere might be only a few hundred kilobases (Nagaki et al., 2004). It has been shown that barley centromeric repeats are neither necessary nor sufficient to establish a centro ...
Cot-1 banding of human chromosomes using fluorescence
... labeling. Clear green signals of probe DNA on the red banded chromosomes can be obtained and the banding resolution is as clear as Q-banding. By this method, we mapped a 4-kb genomic DNA fragment from myelin protein zero (MPZ) gene on chromosome 1q22 to q23 (Fig. 4) that is consistent with our previ ...
... labeling. Clear green signals of probe DNA on the red banded chromosomes can be obtained and the banding resolution is as clear as Q-banding. By this method, we mapped a 4-kb genomic DNA fragment from myelin protein zero (MPZ) gene on chromosome 1q22 to q23 (Fig. 4) that is consistent with our previ ...
Biochi~ic~a - ScienceDirect
... are known as the muscle, liver and brain isozymes. The muscle form has been assigned to the long arm of chromosome 11 by high resolution chromosome sorting and DNA spot-blot analysis [4]. The liver form is encoded on chromosome 14 [5] and the brain form maps to chromosome 20 and to an homologous seq ...
... are known as the muscle, liver and brain isozymes. The muscle form has been assigned to the long arm of chromosome 11 by high resolution chromosome sorting and DNA spot-blot analysis [4]. The liver form is encoded on chromosome 14 [5] and the brain form maps to chromosome 20 and to an homologous seq ...
Crossover and Diploid Dominance with Deceptive Fitness
... described by Greene (1996) that follows a specific model known as complete dominance. Partial and "complete" dominance are well established mechanisms from biology that provide an effective explanation for dominance (e.g., see Stansfield (1983)). In addition, this implementation of diploidy requires ...
... described by Greene (1996) that follows a specific model known as complete dominance. Partial and "complete" dominance are well established mechanisms from biology that provide an effective explanation for dominance (e.g., see Stansfield (1983)). In addition, this implementation of diploidy requires ...
Molecular markers in Brassica Rapa
... the inner leaves of both cultivars, and therefore any difference of inner leaf color was obscured. In addition, inner leaves of the Orange queen cultivar also showed the same color phenotype in inner leaves as the OC cultivar before (Fig. 1g) and after (Fig. 1h) sunlight exposure. To test for abioti ...
... the inner leaves of both cultivars, and therefore any difference of inner leaf color was obscured. In addition, inner leaves of the Orange queen cultivar also showed the same color phenotype in inner leaves as the OC cultivar before (Fig. 1g) and after (Fig. 1h) sunlight exposure. To test for abioti ...
Genetic mapping and manipulation: Chapter 2-Two
... chromosome (0.5 × 0.5 = 0.25). The overall percentage of animals with an Unc phenotype will therefore be 4.5 + 20.25 = 24.75%. Finally, the percentage of Unc animals with a ste unc/unc genotype will be 4.5/24.75 = 18.2%. The above determination tells us that if our mutation and marker(s) are 10.0 ma ...
... chromosome (0.5 × 0.5 = 0.25). The overall percentage of animals with an Unc phenotype will therefore be 4.5 + 20.25 = 24.75%. Finally, the percentage of Unc animals with a ste unc/unc genotype will be 4.5/24.75 = 18.2%. The above determination tells us that if our mutation and marker(s) are 10.0 ma ...
Variation in Glutenin Protein Subunits of Wheat
... bonds, such as the anionic detergent sodium dodecyl sulfate (SOS) (Bietz and Wall 1972). Following these treatments the component subunits can be separated by electrophoresis in polyacrylamide gels containing SOS (SOS-PAGE). However, when SOS-PAGE has been used to examine the glutenin subunit compos ...
... bonds, such as the anionic detergent sodium dodecyl sulfate (SOS) (Bietz and Wall 1972). Following these treatments the component subunits can be separated by electrophoresis in polyacrylamide gels containing SOS (SOS-PAGE). However, when SOS-PAGE has been used to examine the glutenin subunit compos ...
`I` to `L` and back again: the odyssey of membrane
... headgroups are indicated with blue ellipsoids and the hydrocarbon chain region is coloured in yellow. (b) L-shape configuration as determined by NMR [7]. The protein has two a-helices with the amphipathic helix parallel to the plane of the bilayer and the hydrophobic helix (Table 1) perpendicular to ...
... headgroups are indicated with blue ellipsoids and the hydrocarbon chain region is coloured in yellow. (b) L-shape configuration as determined by NMR [7]. The protein has two a-helices with the amphipathic helix parallel to the plane of the bilayer and the hydrophobic helix (Table 1) perpendicular to ...
Lecture#3 File
... the end of the thumb. (b) The calcium atom is bound to one of the motifs in the muscle protein troponin-C through six oxygen atoms: 5 from the side chains of Asp and Glu; and one from the main chain. In addition, a water molecule (W) is bound to the calcium atom. (c) Schematic diagram illustrating t ...
... the end of the thumb. (b) The calcium atom is bound to one of the motifs in the muscle protein troponin-C through six oxygen atoms: 5 from the side chains of Asp and Glu; and one from the main chain. In addition, a water molecule (W) is bound to the calcium atom. (c) Schematic diagram illustrating t ...
Tobacco TTG2 regulates vegetative growth and seed production via
... Recently we characterized the role of NtTTG2 in tobacco growth based on increases of the biomass (fresh weight) of plants grown in a medium and in pots, respectively [19]. The contributions of the different NtARF genes to NtTTG2-regulated tobacco growth were evaluated by direct observation of growth ...
... Recently we characterized the role of NtTTG2 in tobacco growth based on increases of the biomass (fresh weight) of plants grown in a medium and in pots, respectively [19]. The contributions of the different NtARF genes to NtTTG2-regulated tobacco growth were evaluated by direct observation of growth ...
Evolutionary analysis of the female
... as evidenced by unusually high sequence coverage resulting from collapsed mapping of slightly divergent copies. HINTW is the only ampliconic gene found on avian W chromosomes, with up to 40 copies observed in chicken16 and with evidence for gene conversion (intrachromosomal recombination) leading to ...
... as evidenced by unusually high sequence coverage resulting from collapsed mapping of slightly divergent copies. HINTW is the only ampliconic gene found on avian W chromosomes, with up to 40 copies observed in chicken16 and with evidence for gene conversion (intrachromosomal recombination) leading to ...
CMBI
... – How do the proteins encoded in genomes interact with each other to produce cells and phenotypes ? – To predict such functional interactions between proteins as there exist e.g. in metabolic pathways, signalling pathways or protein complexes ...
... – How do the proteins encoded in genomes interact with each other to produce cells and phenotypes ? – To predict such functional interactions between proteins as there exist e.g. in metabolic pathways, signalling pathways or protein complexes ...
Gene Section SIL (SCL/TAL1 interrupting locus) Atlas of Genetics and Cytogenetics
... Disease T-cell ALL. This TAL1-SIL fusion transcript is found in approximately 25% of T-ALL patients. Cytogenetics Normal karyotype. Hybrid/Mutated Gene The promoter region of the SCL gene, a hematopoietic transcription factor, and the coding region of the SIL gene are deleted. The molecular result o ...
... Disease T-cell ALL. This TAL1-SIL fusion transcript is found in approximately 25% of T-ALL patients. Cytogenetics Normal karyotype. Hybrid/Mutated Gene The promoter region of the SCL gene, a hematopoietic transcription factor, and the coding region of the SIL gene are deleted. The molecular result o ...
The effecTs of benzoic acid and proTein level on urine ph and
... Twelve hybrids gilts (initial BW 29.9 ± 1.7 kg) were used for evaluation to identify the effect of benzoic acid and dietary protein in the diets on urine pH and ammonia of the slurry by growing piglets. We used two protein concentrations - high protein 18.8 % (HP) and low protein 14.0 % (LP) and two ...
... Twelve hybrids gilts (initial BW 29.9 ± 1.7 kg) were used for evaluation to identify the effect of benzoic acid and dietary protein in the diets on urine pH and ammonia of the slurry by growing piglets. We used two protein concentrations - high protein 18.8 % (HP) and low protein 14.0 % (LP) and two ...
DNA Methylation of Imprinted Loci on Autosomal Chromosomes and
... Genomic imprinting is an epigenetic phenomenon that results in the expression of either the maternally or paternally inherited allele of a subset of genes [1]. In humans, alterations of imprinting patterns gives rise to numerous diseases with well characterized growth phenotypes (Beckwith-Wiedemann ...
... Genomic imprinting is an epigenetic phenomenon that results in the expression of either the maternally or paternally inherited allele of a subset of genes [1]. In humans, alterations of imprinting patterns gives rise to numerous diseases with well characterized growth phenotypes (Beckwith-Wiedemann ...
Point mutation
A point mutation, or single base modification, is a type of mutation that causes a single nucleotide base change, insertion, or deletion of the genetic material, DNA or RNA. The term frameshift mutation indicates the addition or deletion of a base pair. A point mutant is an individual that is affected by a point mutation.Repeat induced point mutations are recurring point mutations, discussed below.