IlllllllllllllIlllllllIllllllllllllllIlllllllllllllllllllllllllllllllllllll
... The tac promoted toxin gene found in pMYC436 40 (pMYC47l, pMYC485, and pMYC1l6l) is constitutive and occurs independently of the concentrations of lac (NRRL deposit no. B-l8292) was cloned as a 4.5 Kbp tose or glucose present in the cell. For example, in the BamHI to PstI fragment in the vector pTJ ...
... The tac promoted toxin gene found in pMYC436 40 (pMYC47l, pMYC485, and pMYC1l6l) is constitutive and occurs independently of the concentrations of lac (NRRL deposit no. B-l8292) was cloned as a 4.5 Kbp tose or glucose present in the cell. For example, in the BamHI to PstI fragment in the vector pTJ ...
Characterization of Star and its interactions with
... 722 bp of 5′ untranslated sequences was cloned into a modified Bluescript (Stratagene) vector that allows for the Star sequences to be isolated as a single KpnI restriction fragment. This KpnI fragment was cloned into the Carnegie 20-derived hsp70 vector HT-4 (Schneuwly et al., 1987) and transformed ...
... 722 bp of 5′ untranslated sequences was cloned into a modified Bluescript (Stratagene) vector that allows for the Star sequences to be isolated as a single KpnI restriction fragment. This KpnI fragment was cloned into the Carnegie 20-derived hsp70 vector HT-4 (Schneuwly et al., 1987) and transformed ...
Journal of Agricultural and Food Chemistry
... Gorinstein et al., 1996). There also are investigations showing comparisons among soybean, oats, and amaranth in relation to nutritional, structural, and functional properties (Gorinstein, 1993; Marcone and Yada, 1992; Segura-Nieto et al., 1994). To date, there is no information on the relative stab ...
... Gorinstein et al., 1996). There also are investigations showing comparisons among soybean, oats, and amaranth in relation to nutritional, structural, and functional properties (Gorinstein, 1993; Marcone and Yada, 1992; Segura-Nieto et al., 1994). To date, there is no information on the relative stab ...
The Arabidopsis stearoyl-acyl carrier protein-desaturase
... encodes a defective stearoyl-acyl carrier proteindesaturase (S-ACP-DES) and consequently accumulates high levels of stearic acid (18:0) and low levels of 18:1. In addition to SSI2, the Arabidopsis genome encodes six S-ACP-DES-like enzymes, the native expression levels of which are unable to compensa ...
... encodes a defective stearoyl-acyl carrier proteindesaturase (S-ACP-DES) and consequently accumulates high levels of stearic acid (18:0) and low levels of 18:1. In addition to SSI2, the Arabidopsis genome encodes six S-ACP-DES-like enzymes, the native expression levels of which are unable to compensa ...
A structural determinant in the uracil DNA glycosylase superfamily
... bidentate hydrogen bonds with the N3 and O4 moieties of the uracil base. Genetic analysis indicates the gain of function for A/U base pairs allows the MUG-K68N mutant to remove uracil incorporated into the genome during DNA replication. The implications of this study in the origin of life are discus ...
... bidentate hydrogen bonds with the N3 and O4 moieties of the uracil base. Genetic analysis indicates the gain of function for A/U base pairs allows the MUG-K68N mutant to remove uracil incorporated into the genome during DNA replication. The implications of this study in the origin of life are discus ...
Creating 3-Dimensional Graph Structures with DNA
... conjugated to paramagnetic beads. The resulting mixture is sequentially treated in a similar way with each vertex speci c oligo. The extraction procedure is undoubtedly less then 100% e ective. With repeated use, errors will tend to accumulate and could result in false positives (i.e., concluding th ...
... conjugated to paramagnetic beads. The resulting mixture is sequentially treated in a similar way with each vertex speci c oligo. The extraction procedure is undoubtedly less then 100% e ective. With repeated use, errors will tend to accumulate and could result in false positives (i.e., concluding th ...
The role of the ovarian tumor locus in Drosophila
... The nature of the somatic signal is not known, but one autosomal and three X-linked loci have been found to be required in female (2X:2A) germ cells for their proper sex determination (reviewed by Pauli and Mahowald, 1990). Mutations in these genes result in either of two different phenotypes: sex-s ...
... The nature of the somatic signal is not known, but one autosomal and three X-linked loci have been found to be required in female (2X:2A) germ cells for their proper sex determination (reviewed by Pauli and Mahowald, 1990). Mutations in these genes result in either of two different phenotypes: sex-s ...
Analysis and Characterization of Nucleic Acids and Proteins
... In end labeling, labeled nucleotides are added to the end of the probe using terminal transferase or T4 polynucleotide kinase. ...
... In end labeling, labeled nucleotides are added to the end of the probe using terminal transferase or T4 polynucleotide kinase. ...
An overview on chemical modification of enzymes. The use of group
... nucleophilicity can be influenced in turn by several factors which, as will be discussed below, can greatly affect the outcome of chemical modification. Two main types of chemical modification can be recognized: one is based on the use of group-specific reagents, and the second utilizes affinity lab ...
... nucleophilicity can be influenced in turn by several factors which, as will be discussed below, can greatly affect the outcome of chemical modification. Two main types of chemical modification can be recognized: one is based on the use of group-specific reagents, and the second utilizes affinity lab ...
A Contribution of the Mitochondrial
... protein). However, latent MgATP-submitochondrial particles (particles containing the inhibi tor protein) pre-incubated under the same conditions are totally inactivated only after 15 min of pre-incubation. When ammonium-Sephadex particles are reconstituted with 20 ^g/ml of purified ATPase inhibitor ...
... protein). However, latent MgATP-submitochondrial particles (particles containing the inhibi tor protein) pre-incubated under the same conditions are totally inactivated only after 15 min of pre-incubation. When ammonium-Sephadex particles are reconstituted with 20 ^g/ml of purified ATPase inhibitor ...
... it is in the hydrophobic core. Since this Thr would form an H-bond to water in the unfolded state, it will cost about 20 kJ/mole of instability since a hydrogen bond to water is broken during folding, but not reformed. (6 pts) Additional factors (1 pt for either, unless the main reason was missed an ...
A Novel Two Domain-Fusion Protein in Cyanobacteria with
... All cyanobacteria examined to date have multiple hli genes (Bhaya et al., 2002; He et al., 2001; Steglich et al., 2006), but they have also been identified on the chloroplast genomes of the red algae Porphyra yezoensis (HYP_537036.1) Hand Cyanidium caldarium (Q9TM07) (Glockner et al., 2000), the gla ...
... All cyanobacteria examined to date have multiple hli genes (Bhaya et al., 2002; He et al., 2001; Steglich et al., 2006), but they have also been identified on the chloroplast genomes of the red algae Porphyra yezoensis (HYP_537036.1) Hand Cyanidium caldarium (Q9TM07) (Glockner et al., 2000), the gla ...
Arabidopsis Branched-Chain Aminotransferase 3
... elongation cycle of aliphatic glucosinolate formation are catalyzed by branched-chain aminotransferases (BCATs) that are encoded by a small gene family of six members. One member of this family, the plastid-located BCAT3, was shown to participate in both amino acid and glucosinolate metabolism. In v ...
... elongation cycle of aliphatic glucosinolate formation are catalyzed by branched-chain aminotransferases (BCATs) that are encoded by a small gene family of six members. One member of this family, the plastid-located BCAT3, was shown to participate in both amino acid and glucosinolate metabolism. In v ...
Identification of a Chloroplast-encoded 9-kDa
... further assigned as [4Fe-4S] clusters (8).The ESR spectrum of center X also had someresemblance to thoseof iron-sulfur centers (9), and Mossbauer spectroscopy suggestedthat center X could be a [4Fe-4S] center (10).However, microwave power saturation studies indicated that centerX was not a typical [ ...
... further assigned as [4Fe-4S] clusters (8).The ESR spectrum of center X also had someresemblance to thoseof iron-sulfur centers (9), and Mossbauer spectroscopy suggestedthat center X could be a [4Fe-4S] center (10).However, microwave power saturation studies indicated that centerX was not a typical [ ...
Brassinosteroids Rescue the Deficiency of CYP90, a Cytochrome
... COP, and FUS genes are thought to code for specific negative regulators of light signaling because the developmental and molecular phenotypes of det, cop, and fus mutants are not altered by the hy mutations of photoreceptors (Quail et al., 1995). Nonetheless, because these mutations have severe plei ...
... COP, and FUS genes are thought to code for specific negative regulators of light signaling because the developmental and molecular phenotypes of det, cop, and fus mutants are not altered by the hy mutations of photoreceptors (Quail et al., 1995). Nonetheless, because these mutations have severe plei ...
p-Adic Modelling of the Genome and the Genetic Code
... main processes to exploit this information. The first one is replication, in which DNA duplicates giving two new DNA containing the same information as the original one. This is possible owing to the fact that each of two chains contains complementary bases of the other one. The second process is re ...
... main processes to exploit this information. The first one is replication, in which DNA duplicates giving two new DNA containing the same information as the original one. This is possible owing to the fact that each of two chains contains complementary bases of the other one. The second process is re ...
Evolutionary origins of invasive populations
... Turelli and Barton 2004). On the one hand, organisms might evolve generalist strategies, which would allow them to prosper across a wide range of conditions. Alternatively, organisms might develop increased evolvability (Boxes 1 and 2), with an increased capacity to adapt rapidly to changing conditi ...
... Turelli and Barton 2004). On the one hand, organisms might evolve generalist strategies, which would allow them to prosper across a wide range of conditions. Alternatively, organisms might develop increased evolvability (Boxes 1 and 2), with an increased capacity to adapt rapidly to changing conditi ...
Article Lateral Gene Transfer and Gene
... that our inability to detect CII activity might be because it was 1) irreversibly inactivated during the preparation of cellular fractions and/or 2) it catalyzes a different reaction. Although we were not able to demonstrate this activity, all critical residues for substrate and cofactor binding are ...
... that our inability to detect CII activity might be because it was 1) irreversibly inactivated during the preparation of cellular fractions and/or 2) it catalyzes a different reaction. Although we were not able to demonstrate this activity, all critical residues for substrate and cofactor binding are ...
PDF - The Journal of General Physiology
... three different dates corresponding to 0, 40, and 50 per cent of the incorporated p3~ decayed. A total of nine different emulsions was counted; the average star size extended from 7.4 to 15.4 rays per star. The average of these determinations for the average number of p~2 atoms per phage particle, N ...
... three different dates corresponding to 0, 40, and 50 per cent of the incorporated p3~ decayed. A total of nine different emulsions was counted; the average star size extended from 7.4 to 15.4 rays per star. The average of these determinations for the average number of p~2 atoms per phage particle, N ...
The human Y chromosome: a sole survivor Noordam, MJ - UvA-DARE
... Over de last decades the Y chromosome has been studied extensively to identify genetic abnormalities that affect spermatogenesis and as a result, male fertility. In 1976, large deletions of the long arm of the Y chromosome (Yq) were first identified, in 1996 followed by the report of three nonoverla ...
... Over de last decades the Y chromosome has been studied extensively to identify genetic abnormalities that affect spermatogenesis and as a result, male fertility. In 1976, large deletions of the long arm of the Y chromosome (Yq) were first identified, in 1996 followed by the report of three nonoverla ...
biomolecules (introduction, structure
... of the amino acid to yield a yellow derivative, the 2,4-dinitrophenyl derivative or DNPamino acid. The compound FDNB will react with the free amino group on the NH2-terminal end of a polypeptide as well as the amino groups of free amino acids. Thus, by reacting a native protein or intact polypeptide ...
... of the amino acid to yield a yellow derivative, the 2,4-dinitrophenyl derivative or DNPamino acid. The compound FDNB will react with the free amino group on the NH2-terminal end of a polypeptide as well as the amino groups of free amino acids. Thus, by reacting a native protein or intact polypeptide ...
Molecular Cell Biology
... anticodon The nucleotide sequence of transfer RNA that forms complementary base pairs with a codon sequence on messenger RNA. antigen A molecule against which an antibody is directed. antiport The transport of two molecules in opposite directions across a membrane. AP endonuclease A DNA repair enzym ...
... anticodon The nucleotide sequence of transfer RNA that forms complementary base pairs with a codon sequence on messenger RNA. antigen A molecule against which an antibody is directed. antiport The transport of two molecules in opposite directions across a membrane. AP endonuclease A DNA repair enzym ...
Article (Published version)
... homozygous mutations in the coding region (E123X), are associated with neonatal diabetes and congenital malabsorption diarrhea as a result of enteric anendocrinosis31,32. Thus, NGN3 is important in human islet and enteroendocrine cell development. Clinical characterization of these patients showed r ...
... homozygous mutations in the coding region (E123X), are associated with neonatal diabetes and congenital malabsorption diarrhea as a result of enteric anendocrinosis31,32. Thus, NGN3 is important in human islet and enteroendocrine cell development. Clinical characterization of these patients showed r ...
Point mutation
A point mutation, or single base modification, is a type of mutation that causes a single nucleotide base change, insertion, or deletion of the genetic material, DNA or RNA. The term frameshift mutation indicates the addition or deletion of a base pair. A point mutant is an individual that is affected by a point mutation.Repeat induced point mutations are recurring point mutations, discussed below.