Pathway/Genome Navigator - Bioinformatics Research Group at SRI
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... You can launch a Google text search from: 1. Search → Google This Site ...
Cloning and Effective Induction of Escherichia coli
... the expression of the desired gene placed under the control of the strong T7, lac or tac promoter. The advantage of using this synthetic inducer is that it cannot be hydrolyzed by β-galactosidase.13 However, the use of IPTG for the large-scale production of human therapeutic proteins is undesirable ...
... the expression of the desired gene placed under the control of the strong T7, lac or tac promoter. The advantage of using this synthetic inducer is that it cannot be hydrolyzed by β-galactosidase.13 However, the use of IPTG for the large-scale production of human therapeutic proteins is undesirable ...
The ZYG-1 kinase, a mitotic and meiotic regulator of centriole
... centrioles are tightly associated with the reproductive capacity of the centrosome (Sluder and Rieder, 1985) and have been implicated in organizing the PCM into a discrete focus (Bobinnec et al., 1998). Surrounding the centrioles is the pericentriolar material or PCM, a complex and dynamic meshwork ...
... centrioles are tightly associated with the reproductive capacity of the centrosome (Sluder and Rieder, 1985) and have been implicated in organizing the PCM into a discrete focus (Bobinnec et al., 1998). Surrounding the centrioles is the pericentriolar material or PCM, a complex and dynamic meshwork ...
Not all mutant phenotypes are equally informative. Forward genetics
... fact: it’s easier to mess things up than to make them better ...
... fact: it’s easier to mess things up than to make them better ...
Mechanisms and impact of genetic recombination in the evolution of
... elements (MGE) such as insertion sequences (IS), integrons, bacteriophages, plasmids and transposons, considered being part of the accessory genome (non-core genome) [52]. Such recombination exchanges can occur between pneumococci or other closely related oral Streptococci including Streptococcus mi ...
... elements (MGE) such as insertion sequences (IS), integrons, bacteriophages, plasmids and transposons, considered being part of the accessory genome (non-core genome) [52]. Such recombination exchanges can occur between pneumococci or other closely related oral Streptococci including Streptococcus mi ...
Molecular Mechanisms of Developmental Review
... lin-14 mRNA, a region with seven copies of a short sequence that could base pair with the lin-4 RNAs (Wightman et al., 1991; Yeh, 1991). In addition, these 3⬘ sequences are sufficient to confer appropriate temporal regulation on the translation of a heterologous gene, implicating these sites as dire ...
... lin-14 mRNA, a region with seven copies of a short sequence that could base pair with the lin-4 RNAs (Wightman et al., 1991; Yeh, 1991). In addition, these 3⬘ sequences are sufficient to confer appropriate temporal regulation on the translation of a heterologous gene, implicating these sites as dire ...
Novel FAD3 Mutant Allele Combinations Produce Soybeans
... 2000). Other soybean germplasm is available with approximately 3% linolenic acid, and those lines have been shown to contain two genes conditioning the trait (Bilyeu et al., 2005; Fehr et al., 1992; Stojsin et al., 1998; Takagi et al., 1999). Independent work on the molecular genetic basis for the l ...
... 2000). Other soybean germplasm is available with approximately 3% linolenic acid, and those lines have been shown to contain two genes conditioning the trait (Bilyeu et al., 2005; Fehr et al., 1992; Stojsin et al., 1998; Takagi et al., 1999). Independent work on the molecular genetic basis for the l ...
Figure 15.6 Nonreplicative transposition allows a transposon to
... that leaves a donor site (usually generating a double-strand break) and moves to a new site. Replicative transposition describes the movement of a transposon by a mechanism in which first it is replicated, and then one copy is transferred to a new site. Resolvase is enzyme activity involved in site- ...
... that leaves a donor site (usually generating a double-strand break) and moves to a new site. Replicative transposition describes the movement of a transposon by a mechanism in which first it is replicated, and then one copy is transferred to a new site. Resolvase is enzyme activity involved in site- ...
Interfering RNA
... • The current knowledge and level of skill in the art is high such that one of ordinary skill in the art would expect at least an RNAi against a known gene, absent evidence to the contrary. • Narrow claims to specific RNAi sequences may be free of the art, where there would be no motivation to modif ...
... • The current knowledge and level of skill in the art is high such that one of ordinary skill in the art would expect at least an RNAi against a known gene, absent evidence to the contrary. • Narrow claims to specific RNAi sequences may be free of the art, where there would be no motivation to modif ...
Amino Acids - faculty at Chemeketa
... Match the end products of digestion with the types of food: 1. amino acids 2. fatty acids and glycerol 3. glucose A. fats B. proteins C. carbohydrates ...
... Match the end products of digestion with the types of food: 1. amino acids 2. fatty acids and glycerol 3. glucose A. fats B. proteins C. carbohydrates ...
Thrombocytosis, Polycythemia Vera, and JAK2 Mutations
... The discovery of JAK2 V617F provided a way to distinguish polycythemia vera and approximately 50% of patients with essential thrombocytosis and primary myelofibrosis, from the many benign and malignant disorders they mimic clinically, albeit not from each other (51). Thus, the presence of the JAK2 V ...
... The discovery of JAK2 V617F provided a way to distinguish polycythemia vera and approximately 50% of patients with essential thrombocytosis and primary myelofibrosis, from the many benign and malignant disorders they mimic clinically, albeit not from each other (51). Thus, the presence of the JAK2 V ...
chapter 13 meiosis and sexual life cycles
... Almost all the DNA in a eukaryotic cell is subdivided into chromosomes in the nucleus. Tiny amounts of DNA are also found in mitochondria and chloroplasts. Every living species has a characteristic number of chromosomes. Humans have 46 chromosomes in almost all of their cells. Each chromos ...
... Almost all the DNA in a eukaryotic cell is subdivided into chromosomes in the nucleus. Tiny amounts of DNA are also found in mitochondria and chloroplasts. Every living species has a characteristic number of chromosomes. Humans have 46 chromosomes in almost all of their cells. Each chromos ...
PDF of original
... in the triplet) correspond to the same amino acid; often XYA and XYG correspond to the same amino acid; sometimes XYG alone corresponds to an amino acid. For eight amino acids, U, C, A, or G may occupy the third position of synonym codons. Alternate bases also may occupy the first position of synony ...
... in the triplet) correspond to the same amino acid; often XYA and XYG correspond to the same amino acid; sometimes XYG alone corresponds to an amino acid. For eight amino acids, U, C, A, or G may occupy the third position of synonym codons. Alternate bases also may occupy the first position of synony ...
15.13 Spm elements influence gene expression
... Composite transposons have IS modules Transposition occurs by both replicative and nonreplicative mechanisms 15.5 Transposons cause rearrangement of DNA 15.6 Common intermediates for transposition 15.7 Replicative transposition proceeds through a cointegrate 15.8 Nonreplicative transposition proceed ...
... Composite transposons have IS modules Transposition occurs by both replicative and nonreplicative mechanisms 15.5 Transposons cause rearrangement of DNA 15.6 Common intermediates for transposition 15.7 Replicative transposition proceeds through a cointegrate 15.8 Nonreplicative transposition proceed ...
A mosaic infertile case of isodicentricY
... Objective: Structural Y chromosome abnormalities such as; DAZ, AZF and sY152 deletion play a key role in the genetic etiology of male infertility. Here we used comparable techniques to determine the characteristics of Y chromosome microdeletions and/or duplications in an infertile man in the current ...
... Objective: Structural Y chromosome abnormalities such as; DAZ, AZF and sY152 deletion play a key role in the genetic etiology of male infertility. Here we used comparable techniques to determine the characteristics of Y chromosome microdeletions and/or duplications in an infertile man in the current ...
Crystal structure of yeast hexokinase Pl in complex
... is required for catabolite repression by glucose of expression of other genes.4,5 The yeast HKs are known to exist as phosphoproteins in vitro6 and in vivo7; in vivo phosphorylation site was identified as Ser15.8 It was demonstrated that the transformed cells with the hexokinase PII S15A mutant gene ...
... is required for catabolite repression by glucose of expression of other genes.4,5 The yeast HKs are known to exist as phosphoproteins in vitro6 and in vivo7; in vivo phosphorylation site was identified as Ser15.8 It was demonstrated that the transformed cells with the hexokinase PII S15A mutant gene ...
Consensus temporal order of amino acids and evolution
... important properties not visible in individual rankings by any of the initial criteria. Nine amino acids of the Miller's imitation of primordial environment are all ranked as topmost (G, A, V, D, E, P, S, L, T). This result does not change even after several criteria related to Miller's data are exc ...
... important properties not visible in individual rankings by any of the initial criteria. Nine amino acids of the Miller's imitation of primordial environment are all ranked as topmost (G, A, V, D, E, P, S, L, T). This result does not change even after several criteria related to Miller's data are exc ...
209 Original Scientific Article THE INFLUENCE OF
... Taken together, these results suggest that subsequent to embryonic genome activation, the DNMT1 and DNMT3a genes transcription is silenced, likely as a response to the insufficient demethylation of their promoter domains (12). ...
... Taken together, these results suggest that subsequent to embryonic genome activation, the DNMT1 and DNMT3a genes transcription is silenced, likely as a response to the insufficient demethylation of their promoter domains (12). ...
Prediction of mitochondrial proteins of malaria parasite
... protein provides more information than composition of whole protein (Kumar et al. 2006). Based on this observation, methods have been developed using split amino acid composition (Verma et al. 2008). In SAAC, first sequence is divided in parts then composition of each part is computed separately and ...
... protein provides more information than composition of whole protein (Kumar et al. 2006). Based on this observation, methods have been developed using split amino acid composition (Verma et al. 2008). In SAAC, first sequence is divided in parts then composition of each part is computed separately and ...
Cyanuric acid hydrolase: evolutionary innovation by structural
... by AtzD is likely to be magnesium (Dudev et al., 1999; Fig. 3D). Anomalous scattering data show that the native metal ion is either magnesium or sodium (Supplemental Fig. S1). Unfortunately, it is not possible to distinguish between Na+ and Mg2+ by anomalous scattering as both cations have an identi ...
... by AtzD is likely to be magnesium (Dudev et al., 1999; Fig. 3D). Anomalous scattering data show that the native metal ion is either magnesium or sodium (Supplemental Fig. S1). Unfortunately, it is not possible to distinguish between Na+ and Mg2+ by anomalous scattering as both cations have an identi ...
Complete Sequence of the Mitochondrial DNA of
... extremely small genome size, (2) absence of lengthy noncoding regions (with the largest intergenic spacer being only 46 nt), (3) size reduction of encoded genes, and (4) many overlapping genes. Several tRNA genes exhibit bizarre secondary structures with reduced T or D stems, and many tRNA genes hav ...
... extremely small genome size, (2) absence of lengthy noncoding regions (with the largest intergenic spacer being only 46 nt), (3) size reduction of encoded genes, and (4) many overlapping genes. Several tRNA genes exhibit bizarre secondary structures with reduced T or D stems, and many tRNA genes hav ...
Lecture 2
... observations (gross errors/non-responding probes, etc) Possibly a further round of normalization (probe set level) as lab/cohort/batch effects are frequently still visible ...
... observations (gross errors/non-responding probes, etc) Possibly a further round of normalization (probe set level) as lab/cohort/batch effects are frequently still visible ...
Point mutation
A point mutation, or single base modification, is a type of mutation that causes a single nucleotide base change, insertion, or deletion of the genetic material, DNA or RNA. The term frameshift mutation indicates the addition or deletion of a base pair. A point mutant is an individual that is affected by a point mutation.Repeat induced point mutations are recurring point mutations, discussed below.