How to measure chromatin modifications
... more expensive traditional sequencing methods. Options ChIP–Seq does not require pre-selection of genomic regions whereas ChIP–chip can only analyse the portion of the genome on a microarray. Although ChIP–SAGE can be used to study entire genomes, it is limited to regions that have recognition sites ...
... more expensive traditional sequencing methods. Options ChIP–Seq does not require pre-selection of genomic regions whereas ChIP–chip can only analyse the portion of the genome on a microarray. Although ChIP–SAGE can be used to study entire genomes, it is limited to regions that have recognition sites ...
File
... 1. True. The observation that haploid cells have half the DNA of diploid cells may have been circumstantial. However, when combined with the experimental evidence obtained by Griffith and Hershey and Chase, the observation that haploid cells have half the DNA of diploid cells helped to confirm that ...
... 1. True. The observation that haploid cells have half the DNA of diploid cells may have been circumstantial. However, when combined with the experimental evidence obtained by Griffith and Hershey and Chase, the observation that haploid cells have half the DNA of diploid cells helped to confirm that ...
Folate
... homocysteine, also prevents build up of dementias, which can cause Alzheimer’s AKA Lou Gehrig’s disease. Folate also helps maintain the nervous sytem by keeping it functioning through the synthesis of the neurotransmitters that send signals throughout the nervous system Folic acid is especially impo ...
... homocysteine, also prevents build up of dementias, which can cause Alzheimer’s AKA Lou Gehrig’s disease. Folate also helps maintain the nervous sytem by keeping it functioning through the synthesis of the neurotransmitters that send signals throughout the nervous system Folic acid is especially impo ...
review article
... including those of biological warfare, quorum sensing, intercellular signalling and regulation of gene expression. It is therefore not surprising that several transporter families have evolved to control and facilitate exchange of these substances between cells and their environment, between cells a ...
... including those of biological warfare, quorum sensing, intercellular signalling and regulation of gene expression. It is therefore not surprising that several transporter families have evolved to control and facilitate exchange of these substances between cells and their environment, between cells a ...
A New Species of Actinomycete, Amycolata alni
... more) were detected in all of the strains, including strain DSM 535T, although these thickenings were observed more often in fresh isolates than in strains stored in culture collections for a long time. In stained preparations, transverse and longitudinal septa were observed within these structures. ...
... more) were detected in all of the strains, including strain DSM 535T, although these thickenings were observed more often in fresh isolates than in strains stored in culture collections for a long time. In stained preparations, transverse and longitudinal septa were observed within these structures. ...
9c63$$mr30 Black separation
... activities were observed from the combination of cystalysin plus either of the compounds (table 1). Cystalysin obeys Michaelis-Menten kinetics, with cysteine as the substrate (figure 5). The Km was determined to be 3.4 mM, and the Vmax of 2.52 corresponds to a kcat of 12 sec01 at room temperature. T ...
... activities were observed from the combination of cystalysin plus either of the compounds (table 1). Cystalysin obeys Michaelis-Menten kinetics, with cysteine as the substrate (figure 5). The Km was determined to be 3.4 mM, and the Vmax of 2.52 corresponds to a kcat of 12 sec01 at room temperature. T ...
Improvement of poly-γ-glutamic acid (PGA)
... Strain improvement (P<0.05). Those of nine mutant isolates were quantified for PGA production in PGA broth at 37oC for 36 h. Five isolates (NTG-17, NTG-53, NTG-88, NTG-132 and NTG-146) produced the PGA higher than the wild type (P<0.05). In addition, the selected five NTG mutants provided the maximu ...
... Strain improvement (P<0.05). Those of nine mutant isolates were quantified for PGA production in PGA broth at 37oC for 36 h. Five isolates (NTG-17, NTG-53, NTG-88, NTG-132 and NTG-146) produced the PGA higher than the wild type (P<0.05). In addition, the selected five NTG mutants provided the maximu ...
Molecular Design of Expression Systems
... In order to maximize product synthesis, cells are typically grown to high densities with minimal cloned gene expression followed by a production phase in which high expression results from induction or derepresSeveral different sion of cloned gene transcripti~n.'~?~~ promoter-operator systems, inclu ...
... In order to maximize product synthesis, cells are typically grown to high densities with minimal cloned gene expression followed by a production phase in which high expression results from induction or derepresSeveral different sion of cloned gene transcripti~n.'~?~~ promoter-operator systems, inclu ...
12.04.518 Preconception Testing for Carrier Status of Genetic
... in all 4 α-globin genes (--/--), resulting in absent production of α-globin chains. This condition causes hydrops fetalis, which often leads to intrauterine death, or death shortly after birth. There are also increased complications of pregnancy for a woman carrying a fetus with hydrops fetalis. The ...
... in all 4 α-globin genes (--/--), resulting in absent production of α-globin chains. This condition causes hydrops fetalis, which often leads to intrauterine death, or death shortly after birth. There are also increased complications of pregnancy for a woman carrying a fetus with hydrops fetalis. The ...
Oxalate decarboxylase of the white-rot fungus
... Dichomitus squalens, and cloned the corresponding Ds-odc gene. The primary structure of the Ds-ODC protein contains two conserved Mn-binding cupin motifs, but at the N-terminus, a unique, approximately 60 aa alanine-serine-rich region is found. Real-time quantitative RT-PCR analysis confirmed gene e ...
... Dichomitus squalens, and cloned the corresponding Ds-odc gene. The primary structure of the Ds-ODC protein contains two conserved Mn-binding cupin motifs, but at the N-terminus, a unique, approximately 60 aa alanine-serine-rich region is found. Real-time quantitative RT-PCR analysis confirmed gene e ...
Proficiency Test Lyon 2008
... - 12 Mutation analysis of GK gene on chromosome X (Dr van Amstel, Utrecht) identified c.883+1G>A a splice site mutation in intron 10 which disrupts the donor splice site (AAAAATACGTGAGTTT) and is deleterious for correct splicing of the mRNA. Complex form was also excluded by normal endocrinological ...
... - 12 Mutation analysis of GK gene on chromosome X (Dr van Amstel, Utrecht) identified c.883+1G>A a splice site mutation in intron 10 which disrupts the donor splice site (AAAAATACGTGAGTTT) and is deleterious for correct splicing of the mRNA. Complex form was also excluded by normal endocrinological ...
Phylogenetic analysis of members of the genus Rickettsia using the
... al., 1997). When the degree of infection was optimal, as estimated by Gimenez staining, rickettsiae were harvested and cultures were centrifuged at 12 000 g for 10 min, resuspended in medium and stored at k70 mC until nucleic acid purification was performed. Nucleic acid purification and PCR amplifi ...
... al., 1997). When the degree of infection was optimal, as estimated by Gimenez staining, rickettsiae were harvested and cultures were centrifuged at 12 000 g for 10 min, resuspended in medium and stored at k70 mC until nucleic acid purification was performed. Nucleic acid purification and PCR amplifi ...
A Review of Issues of Dietary Protein Intake in Humans
... (26). Catabolism of amino acids occurs in the liver, which contains the urea cycle (26), however the rate of conversion of amino acid derived ammonia to urea is limited. Rudman et al. (27) found that the maximal rate of urea excretion (MRUE) in healthy individuals was 55 mg urea N ∙ h-1 ∙ kg-0.75, w ...
... (26). Catabolism of amino acids occurs in the liver, which contains the urea cycle (26), however the rate of conversion of amino acid derived ammonia to urea is limited. Rudman et al. (27) found that the maximal rate of urea excretion (MRUE) in healthy individuals was 55 mg urea N ∙ h-1 ∙ kg-0.75, w ...
Participation of DDDD and KPAR
... Mercuric reductase (MerA) is an essential enzyme for the survival of microorganisms that reside in environments containing mercuric compounds. The enzyme converts the extremely toxic mercuric ions (Hg2+) into the less toxic volatile elemental mercury form (Hg 0). A novel MerA molecule that has under ...
... Mercuric reductase (MerA) is an essential enzyme for the survival of microorganisms that reside in environments containing mercuric compounds. The enzyme converts the extremely toxic mercuric ions (Hg2+) into the less toxic volatile elemental mercury form (Hg 0). A novel MerA molecule that has under ...
Lecture-Mic 623-Plasmids-Listeria - Home
... rolling-circle mechanism. The rep gene encoded a protein identical to RepU, the protein required for initiation of the replication of plasmids pTB913 from a thermophilic Bacillus sp. and pUB110 from Staphylococcus aureus. ...
... rolling-circle mechanism. The rep gene encoded a protein identical to RepU, the protein required for initiation of the replication of plasmids pTB913 from a thermophilic Bacillus sp. and pUB110 from Staphylococcus aureus. ...
16p13 deletions FTNP Right click and
... A heart condition in 3/12 children. One boy was born with a persistent ductus arteriosus (a persisting structure of the fetal circulation) that was corrected at five months; one baby had a defect that led to heart failure but was surgically repaired; and one was born with a complex of structural a ...
... A heart condition in 3/12 children. One boy was born with a persistent ductus arteriosus (a persisting structure of the fetal circulation) that was corrected at five months; one baby had a defect that led to heart failure but was surgically repaired; and one was born with a complex of structural a ...
Old and New TB Drugs: Mechanisms of Action and
... occurring in 50–80% of cases, thus reducing the ability of the catalase-peroxidase to activate the INH pro-drug. The katG gene is located in a highly variable and unstable region of the M. tuberculosis genome, with missense and nonsense mutations, insertions, deletions, truncation and, more rarely, ...
... occurring in 50–80% of cases, thus reducing the ability of the catalase-peroxidase to activate the INH pro-drug. The katG gene is located in a highly variable and unstable region of the M. tuberculosis genome, with missense and nonsense mutations, insertions, deletions, truncation and, more rarely, ...
Translational Repression of C. elegans p53 by GLD
... 2003; Vassilev et al., 2004). Such a therapeutic approach highlights the need to uncover additional pathways and mechanisms that negatively regulate p53 levels or activity. Most studies on p53 signaling have been conducted in cell culture-based systems, and their translation into mouse models is oft ...
... 2003; Vassilev et al., 2004). Such a therapeutic approach highlights the need to uncover additional pathways and mechanisms that negatively regulate p53 levels or activity. Most studies on p53 signaling have been conducted in cell culture-based systems, and their translation into mouse models is oft ...
Different strategies of osmoadaptation in the closely
... become dehydrated. This will eventually disrupt cellular metabolism, and so is used in food conservation by pickling. One strategy to thrive in such environments involves the production of so-called osmolytes to maintain osmotic equilibrium across the cytoplasmic membrane. Osmolytes are organic comp ...
... become dehydrated. This will eventually disrupt cellular metabolism, and so is used in food conservation by pickling. One strategy to thrive in such environments involves the production of so-called osmolytes to maintain osmotic equilibrium across the cytoplasmic membrane. Osmolytes are organic comp ...
16p13 deletions FTNW - Unique The Rare Chromosome Disorder
... The main symptoms of ATR-16 syndrome are a blood disorder and usually mild to moderate learning difficulties. However, small deletions around the genes whose absence causes the blood disorder can leave no other effects at all (Horsley 2001). Babies may also have some unusual facial features, but the ...
... The main symptoms of ATR-16 syndrome are a blood disorder and usually mild to moderate learning difficulties. However, small deletions around the genes whose absence causes the blood disorder can leave no other effects at all (Horsley 2001). Babies may also have some unusual facial features, but the ...
Intermediate Alleles - Huntington Society of Canada
... passed down in families from generation to generation. While this is most often the case, scientists have now determined that in some families, HD has not been present for generations; instead the disease appears in the family unexpectedly. Scientists call this single case of HD in a family with no ...
... passed down in families from generation to generation. While this is most often the case, scientists have now determined that in some families, HD has not been present for generations; instead the disease appears in the family unexpectedly. Scientists call this single case of HD in a family with no ...
Point mutation
A point mutation, or single base modification, is a type of mutation that causes a single nucleotide base change, insertion, or deletion of the genetic material, DNA or RNA. The term frameshift mutation indicates the addition or deletion of a base pair. A point mutant is an individual that is affected by a point mutation.Repeat induced point mutations are recurring point mutations, discussed below.