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The Emerging Age of Cell-free Synthetic Biology
The Emerging Age of Cell-free Synthetic Biology

... There is rich diversity of CF systems, reaction modes, and preparation techniques that provide many options for applying CF synbio. For example, there have been considerable well-described iterations towards streamlining preparation of E. coli-based CF extract in the last 30 years [61, 62]. Some pre ...
Final Exam - UC Davis Plant Sciences
Final Exam - UC Davis Plant Sciences

... Arginine can be synthesized from ornithine in the urea cycle. Therefore, arginine is an essential amino acid only under certain circumstances when the metabolic demand for arginine is exceedingly high, such as during childhood, pregnancy, or recovery from illnesses. Ornithine (see below, compound on ...
Nitrogen Assimilation 1. Introduction and Overview Importance of
Nitrogen Assimilation 1. Introduction and Overview Importance of

... 3.  principles  of  synthesis  of  amino  acids     4.  nitrogen  fixation  as  a  special  case  (legumes)   ...
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Acid – base balance
Acid – base balance

... Why is pH so important ? ...
AP Biology Cellular Respiration Notes 9.1
AP Biology Cellular Respiration Notes 9.1

... reactions of an electron transport chain. (Creating a H+ gradient and using it to drive ATP Synthase.) 9.15 In general terms, explain how the exergonic “slide” of electrons down the electron transport chain is coupled to the endergonic production of ATP by chemiosmosis. 1. Electrons are made availab ...
Chem 306 Ch 19 Enzymes Spring 2007
Chem 306 Ch 19 Enzymes Spring 2007

... How is enzyme activity measured? • Experiments that measure enzyme activity are called assays. • Assays for blood enzymes are performed in medical laboratories – Some assays determine how fast the characteristic color of a product forms or the color of a substrate decreases ...
CH_16_3_Formation_Peptides
CH_16_3_Formation_Peptides

... • is named with names of all amino acids in the peptide using a -yl ending for the name • is named using the full amino acid name for the amino acid at the the C terminal N Terminal ...
Biological Molecules Ch 2: Chemistry Comes to Life
Biological Molecules Ch 2: Chemistry Comes to Life

...  This is determined by the primary structure.  Hydrogen bonding between amino acids in the protein shape α-helix or β-pleated sheets.  A polypeptide chain can contain both α-helix and βpleated sheets ...
Disallowed Ramachandran Conformations of Amino Acid Residues
Disallowed Ramachandran Conformations of Amino Acid Residues

... Is the unusual stereochemistry observed at the disallowed residues an intrinsic feature dictated by the structural environment or is it a lingering artifact of the structure determination process, despite a rigorous attempt to screen the data base? (Kleywegt & Jones, 1995). The availability of struc ...
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Organization of Organic Matrix Components in Mineralized Tissues`
Organization of Organic Matrix Components in Mineralized Tissues`

... depressa [Weiner and Erez, 1984]), an echiIn many mineralized tissues, the organic noderm (the sea urchin Paracentrotus livimatrix forms a two- or three-dimensional dans [Weiner and Erez, 1984]) and a molstructure onto which or into which the lusk (the cephalopod Nautilus repertus crystals grow. Mic ...
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Slide 1

... (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches. BLAST can be used to infer functional and evolutionary relationships between sequences as well as help ident ...
Identification, Expression and Characterization of Archaeal
Identification, Expression and Characterization of Archaeal

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Homework # 9 Citric Acid Cycle, electron transport Chain, and
Homework # 9 Citric Acid Cycle, electron transport Chain, and

... Apparently most amino acids may be deaminated but this is a significant reaction only for glutamic acid. If this is true, then how are the other amino acids deaminated? The answer is that a combination of transamination and deamination of glutamic acid occurs which is a recycling type of reaction fo ...
Effect of essential and non-essential amino acid addition to a
Effect of essential and non-essential amino acid addition to a

... ABSTRACT: Pigs fitted with ileal T-cannula in the terminal ileum were used to study the effect of synthetic amino acids (AA) added to a nitrogen-free diet on endogenous losses. We compared the obtained data with data from other research centres in the world that are focusing on these problems. In th ...
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Lecture 4

... 2. General regulation of plasma glucose Pancreas -----> alpha and beta cells Fig. 9-1 and 9-2 (Metab.) 3. Normal and abnormal plasma glucose levels -----> endocrine dysfunction/cell response dysfunction Fig 9-4 (Metab.) 4. Metabolic consequences of insulin insufficiency Fig. 9-3 (Metab) Types of Dia ...
SPINDLY, a tetratricopeptide repeat protein involved in
SPINDLY, a tetratricopeptide repeat protein involved in

... shows that, as with the weaker spy-i allele (5), spy-4 is largely but not completely epistatic to gal-2. spy-4 gal-2 double mutants do not require exogenous GA for seed germination, petal development, and male fertility; however, spy-4 gal-2 double mutant plant height is less than that of spy-4 sing ...
UNIT 3 – PHOTOSYNTHESIS AND CELLULAR RESPIRATION
UNIT 3 – PHOTOSYNTHESIS AND CELLULAR RESPIRATION

... enzymes to intermediates of glycolysis and the citric acid cycle. Before amino acids can enter these processes, deamination must take place – the amino groups must be removed. The nitrogen containing wastes are excreted in the form of ammonia, urea or uric acid.  Fats are also digested and absorbed ...
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Practical part

... Biochemistry of mineralization of tooth enamel. Biochemical events in process of demineralization and tooth decay ...
Conserved Positions for Ribose Recognition: Importance of Water
Conserved Positions for Ribose Recognition: Importance of Water

Slide 1
Slide 1

... (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches. BLAST can be used to infer functional and evolutionary relationships between sequences as well as help ident ...
The maize ID1 flowering time regulator is a zinc finger protein with
The maize ID1 flowering time regulator is a zinc finger protein with

... sequence of seven amino acids known as the H/C link. In these cluster-type zinc ®nger proteins key amino acid residues in the a-helix of each ®nger interact with a triplet of base pairs (5,6). In plants, the C2H2 class of zinc ®nger proteins is one of the largest families of transcription factors to ...
Multiple Sequence Motifs in the Rubisco Small
Multiple Sequence Motifs in the Rubisco Small

... motifs required for protein import into chloroplasts (Lee et al., 2006). Here, in a similar approach, we identified sequence motifs in the E1a-tp critical for protein import into chloroplasts. Serial Ala scanning mutants, E1a[T2A], E1a[T3A], E1a[T4A], E1a[T5A], and E1a[T6A], were generated by replac ...
Cloning and sequencing of a gene encoding acidophilic amylase
Cloning and sequencing of a gene encoding acidophilic amylase

... conditions (data not shown). Therefore, the 160 kDa form was apparently not a disulphide-linkeddimer of the 90 kDa form. The amount of protein in the 90 kDa and 160 kDa bands was approximately the same, yet the starch-degrading activity of the 160 kDa protein was much higher (Fig. 1). Thus, the 160 ...
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Proteolysis



Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.
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