Cloning and sequencing of a gene encoding acidophilic amylase
... conditions (data not shown). Therefore, the 160 kDa form was apparently not a disulphide-linkeddimer of the 90 kDa form. The amount of protein in the 90 kDa and 160 kDa bands was approximately the same, yet the starch-degrading activity of the 160 kDa protein was much higher (Fig. 1). Thus, the 160 ...
... conditions (data not shown). Therefore, the 160 kDa form was apparently not a disulphide-linkeddimer of the 90 kDa form. The amount of protein in the 90 kDa and 160 kDa bands was approximately the same, yet the starch-degrading activity of the 160 kDa protein was much higher (Fig. 1). Thus, the 160 ...
EFFECT OF COOKING AND ROASTING ON THE AMINO ACID
... is the raw sample. About 350 g of the dried groundnut pods were put into an iron pot and mixed with clean fine sand and stirred to prevent burning of the sample and to ensure uniform distribution of heat. The groundnut pods were roasted for about 30 min at 120-130°C using Gallenkamp thermostat hot p ...
... is the raw sample. About 350 g of the dried groundnut pods were put into an iron pot and mixed with clean fine sand and stirred to prevent burning of the sample and to ensure uniform distribution of heat. The groundnut pods were roasted for about 30 min at 120-130°C using Gallenkamp thermostat hot p ...
Biochem Jeopardy
... A: What is saturated – all single bonds, bonded to max. number of hydrogen atoms; unsaturated – one double bond, not holding max number of H atoms? S2C06 Jeopardy Review ...
... A: What is saturated – all single bonds, bonded to max. number of hydrogen atoms; unsaturated – one double bond, not holding max number of H atoms? S2C06 Jeopardy Review ...
On the nature of cavities on protein surfaces: Application to the
... • Out 18 attributes out of 408 used, were found to be significant predictors of drug binding cavities. • It follows from the above that drug binding cavities are large, deep, have an intricate curvature profile, are rigid, and have a relatively small number of prolines, as well as amino acids with s ...
... • Out 18 attributes out of 408 used, were found to be significant predictors of drug binding cavities. • It follows from the above that drug binding cavities are large, deep, have an intricate curvature profile, are rigid, and have a relatively small number of prolines, as well as amino acids with s ...
PMC-AT Enzyme Engineering Research Overview.
... Make competent cells for the host strain so they can take up DNA. Transform the wild-type plasmid into host as positive control. Transform the mutant plasmids into host cells. Assay whether the transformants could grow on carbon-limited media, such as with acetate or propionate as sources. If there ...
... Make competent cells for the host strain so they can take up DNA. Transform the wild-type plasmid into host as positive control. Transform the mutant plasmids into host cells. Assay whether the transformants could grow on carbon-limited media, such as with acetate or propionate as sources. If there ...
Biology
... B. oxygen produced by the green sulfur bacteria during photosynthesis would be used by the central bacterium. C. the central bacterium relies on the green sulfur bacteria for organic material for respiration and it supplies the green sulfur bacteria with carbon dioxide. D. the central bacterium woul ...
... B. oxygen produced by the green sulfur bacteria during photosynthesis would be used by the central bacterium. C. the central bacterium relies on the green sulfur bacteria for organic material for respiration and it supplies the green sulfur bacteria with carbon dioxide. D. the central bacterium woul ...
Lecture 36 - Lipid Metabolism 2
... Key Concepts in Lipid Metabolism • Fatty acid synthesis takes place in the cytosol, uses NADPH as coenzyme in redox reactions, and the building block is malonyl-CoA. • Acetyl-CoA carboxylase is the key regulated enzyme in fatty acid synthesis and is responsible for generating malonyl-CoA in a carbo ...
... Key Concepts in Lipid Metabolism • Fatty acid synthesis takes place in the cytosol, uses NADPH as coenzyme in redox reactions, and the building block is malonyl-CoA. • Acetyl-CoA carboxylase is the key regulated enzyme in fatty acid synthesis and is responsible for generating malonyl-CoA in a carbo ...
Chapter 10 - People Server at UNCW
... • It is a triplet code • Three successive mRNA bases form a codon • There are 64 codons • Altering the DNA sequence by one or two bases produced a different amino acid sequence due to disruption in the reading frame • Adding a base at one point and deleting a base at another point disrupted the read ...
... • It is a triplet code • Three successive mRNA bases form a codon • There are 64 codons • Altering the DNA sequence by one or two bases produced a different amino acid sequence due to disruption in the reading frame • Adding a base at one point and deleting a base at another point disrupted the read ...
Full-Text PDF
... growth and development [9], the vacuolar PR1-mCherry signal appeared much stronger. A member of another class of PR proteins, defensin protein PDF1.2, tagged with green fluoresecent protein (GFP) and overexpressed in Arabidopsis, localizes in the endoplasmic reticulum (ER)-derived structures called ...
... growth and development [9], the vacuolar PR1-mCherry signal appeared much stronger. A member of another class of PR proteins, defensin protein PDF1.2, tagged with green fluoresecent protein (GFP) and overexpressed in Arabidopsis, localizes in the endoplasmic reticulum (ER)-derived structures called ...
Functional and structural roles of parasite-specific inserts in the bifunctional S-adenosylmethionine decarboxylase/ornithine
... advantage of these inserts remain unclear. Some speculations for the functions of these inserts include possible interaction sites with as yet undefined regulatory proteins in the parasite, interaction sites with host proteins and a method to evade the host immune response (Li and Baker, 1998; Schof ...
... advantage of these inserts remain unclear. Some speculations for the functions of these inserts include possible interaction sites with as yet undefined regulatory proteins in the parasite, interaction sites with host proteins and a method to evade the host immune response (Li and Baker, 1998; Schof ...
Chapter 25 Chapter Topics Fatty Acid Biosynthesis
... synthesis of LDL receptors. Only works for heterozygotes because homozygotes have not receptor to stimulate. Elevated HDL seems to mitigate the plaque accumulation by acting to remove cholestrol from peripheral cells, returning it to the ...
... synthesis of LDL receptors. Only works for heterozygotes because homozygotes have not receptor to stimulate. Elevated HDL seems to mitigate the plaque accumulation by acting to remove cholestrol from peripheral cells, returning it to the ...
Lecture 8: 9/9
... which is a linear polysaccharide chain cross‐linked by short peptides. Glycopeptide transpeptidase catalyzes the peptide cross‐links. The transpeptidase reaction proceeds through an acyl‐enzyme intermediate. ...
... which is a linear polysaccharide chain cross‐linked by short peptides. Glycopeptide transpeptidase catalyzes the peptide cross‐links. The transpeptidase reaction proceeds through an acyl‐enzyme intermediate. ...
Analysis of amino acids and peptide primary structure determination
... • The amino acid has no NET charge at its pI; it has one positive and one negative charge. • At a pH less than the value of the isoelectric point, the amino acid is protonated and has a POSITIVE charge; at a pH greater than the pI the amino acid is deprotonated and has a NEGATIVE charge. O C H3N ...
... • The amino acid has no NET charge at its pI; it has one positive and one negative charge. • At a pH less than the value of the isoelectric point, the amino acid is protonated and has a POSITIVE charge; at a pH greater than the pI the amino acid is deprotonated and has a NEGATIVE charge. O C H3N ...
Amino acid composition of the aerial part of G. pratense L., G
... Typically, high performance liquid chromatography (HPLC) is used for the analysis of amino acids [3]. Amino acids lack chromophores and do not give a UV-Vis response, hence amino acids require derivatization prior to HPLC analysis performed with UV-Vis detectors. A method to determine free amino aci ...
... Typically, high performance liquid chromatography (HPLC) is used for the analysis of amino acids [3]. Amino acids lack chromophores and do not give a UV-Vis response, hence amino acids require derivatization prior to HPLC analysis performed with UV-Vis detectors. A method to determine free amino aci ...
COS 551: Introduction to Computational Molecular Biology Lecturer: Mona Singh
... 2) Search for seed words in the database – these are called“hits.” There are a few ways to do this quickly - one is to build a deterministic finite automata that accepts for the appropriate words, and we search through the entire sequence database. Alternatively, we may do a table look up (which is ...
... 2) Search for seed words in the database – these are called“hits.” There are a few ways to do this quickly - one is to build a deterministic finite automata that accepts for the appropriate words, and we search through the entire sequence database. Alternatively, we may do a table look up (which is ...
File
... • The metabolic changes on the first day of starvation are like those after an overnight fast. The low blood-sugar level leads to decreased secretion of insulin and increased secretion of glucagon. The dominant metabolic processes are the mobilization of triacylglycerols in adipose tissue and gluco ...
... • The metabolic changes on the first day of starvation are like those after an overnight fast. The low blood-sugar level leads to decreased secretion of insulin and increased secretion of glucagon. The dominant metabolic processes are the mobilization of triacylglycerols in adipose tissue and gluco ...
Chapter 2: Biochemistry Problems
... If you were a biochemist, you would study chemical substances and vital processes that occur in living organisms. You might study macromolecules such as lipids and phospholipids, carbohydrates, proteins, or nucleic acids. You might study pathways such as glycolysis or photosynthesis, or any other me ...
... If you were a biochemist, you would study chemical substances and vital processes that occur in living organisms. You might study macromolecules such as lipids and phospholipids, carbohydrates, proteins, or nucleic acids. You might study pathways such as glycolysis or photosynthesis, or any other me ...
Microsoft Word
... based on the concept of ‘hybrid helices’. Hybrid helices were designed based on a combination of two or more different types of homologous and hybrid peptides, e.g. peptides and /- and /-hybrid peptides10,17 within the same oligomer. Based on the above design, peptides 42 to 44 were prepared, f ...
... based on the concept of ‘hybrid helices’. Hybrid helices were designed based on a combination of two or more different types of homologous and hybrid peptides, e.g. peptides and /- and /-hybrid peptides10,17 within the same oligomer. Based on the above design, peptides 42 to 44 were prepared, f ...
E-mail: - HAL
... the numerous attempts to depict the connecting regions more precisely.12-16 Many research groups have designed fragment libraries or structural alphabets to try to describe the local structural features of known protein structures more accurately.17-29 These libraries or alphabets correspond to fini ...
... the numerous attempts to depict the connecting regions more precisely.12-16 Many research groups have designed fragment libraries or structural alphabets to try to describe the local structural features of known protein structures more accurately.17-29 These libraries or alphabets correspond to fini ...
La comparaison de séquence
... Scoring matrices are created based on biological evidence. Alignments can be thought of as two sequences that differ due to mutations in the sequence. Some of these mutations have little effect on the organism’s function, therefore some penalties will be less harsh than others. ...
... Scoring matrices are created based on biological evidence. Alignments can be thought of as two sequences that differ due to mutations in the sequence. Some of these mutations have little effect on the organism’s function, therefore some penalties will be less harsh than others. ...
Proteolysis
Proteolysis is the breakdown of proteins into smaller polypeptides or amino acids. Uncatalysed, the hydrolysis of peptide bonds is extremely slow, taking hundreds of years. Proteolysis is typically catalysed by cellular enzymes called proteases, but may also occur by intra-molecular digestion. Low pH or high temperatures can also cause proteolysis non-enzymatically.Proteolysis in organisms serves many purposes; for example, digestive enzymes break down proteins in food to provide amino acids for the organism, while proteolytic processing of a polypeptide chain after its synthesis may be necessary for the production of an active protein. It is also important in the regulation of some physiological and cellular processes, as well as preventing the accumulation of unwanted or abnormal proteins in cells. Consequently, dis-regulation of proteolysis can cause diseases, and is used in some venoms to damage their prey.Proteolysis is important as an analytical tool for studying proteins in the laboratory, as well as industrially, for example in food processing and stain removal.