• Study Resource
  • Explore
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Supplementary Materials and Methods
Supplementary Materials and Methods

... Probe-set identifiers or gene symbols were used to retrieve functional annotation in terms of biological process (BP) and molecular function (MF) from Gene Ontology for the identified signature genes. Genes/probe sets which were not annotated in GO knowledge base were excluded from further analyses. ...
Familial Dysautonomia Is Caused by Mutations of the
Familial Dysautonomia Is Caused by Mutations of the

... highest level of expression was observed in the cerebellum, whose level was set at 1.0; the relative expression levels in the other 19 tissues are shown. The amounts of poly A1 RNA in the tissue samples on the array have been normalized on the basis of eight housekeeping genes. cent studies, however ...
Fundamentals of Protein Interaction Network Mapping
Fundamentals of Protein Interaction Network Mapping

... Low cost Scalable for large-scale screening studies • No need for cell lysis ...
Document
Document

... Foldit is a revolutionary new computer game enabling you to contribute to important scientific research. Join this free online game and help us predict the folds of unsolved proteins as well as designing new proteins to cure diseases. We’re collecting data to find out if humans' pattern-recognition ...
Slides
Slides

... Transmission of signals Defense against intruders ...
Folds
Folds

... protein tertiary structures are divided into five main classes according to the secondary structure content of their domains: all-a domains, all-b domains (b-barrels, e.g. Greek key motif), a+b domains (irregular fashion of arrangement), a/b domains (b-a-b motifs) and “others” each class contains ma ...
Protein Structure and Enzyme Function
Protein Structure and Enzyme Function

... you get “CAR”, which is a completely different thing than a “CAT!” The same is true for protein synthesis. ...
Red meat and protein
Red meat and protein

... Diets must provide the right balance of amino acids and nitrogen essential for the body to be able to synthesise protein for growth and maintenance. Protein quality is a measure of how well or poorly the body can use a given protein to meet its needs. This is dependent on the essential amino acid co ...
Proteins - Many Structures, Many Functions
Proteins - Many Structures, Many Functions

... depends on its ability to recognize and bind to some other molecule. – For example, antibodies bind to particular foreign substances that fit their binding sites. – Enzyme recognize and bind to specific substrates, facilitating a chemical reaction. – Neurotransmitters pass signals from one cell to a ...
Gene Section ALOX15 (arachidonate 15-lipoxygenase) Atlas of Genetics and Cytogenetics
Gene Section ALOX15 (arachidonate 15-lipoxygenase) Atlas of Genetics and Cytogenetics

... Note: Two different 15-Lipoxygenases exist, 15-LOX1 (reticulocyte type) and 15-LOX-2 (epidermis type), differentiated by their tissue expression and a 40% homology at the amino acid level. 15-LOX-1 preferentially oxygenates linoleic acid into 13(S)hydroxyoctadecadienoic acid (13(S)-HPODE) whereas 15 ...
CYP450 Protein Assay – Human Induction Kit Extended Panel
CYP450 Protein Assay – Human Induction Kit Extended Panel

... • Enables measurement of CYP 1A2, 2B6, 2C9, 2C19, 2E1, 3A4 and 3A5 • Stable isotope labeled peptides are supplied as internal standards for quantification of each protein isoform. • The assay can be performed using microsomal or S9 fractions from human hepatocytes or cell lines • Works with current ...
Proteins
Proteins

... common types are the alpha helix and the beta-pleated sheet. The base form a backbone for the protein, with the R groups projecting out from the structure. These structures are maintained by the hydrogen bonds between neighbouring NH and CO groups. (H bonds are weak, but when there are lots of them ...
Protein foods - Deans Community High School
Protein foods - Deans Community High School

... growing and repairing damage. We can get protein into our bodies by eating the foods shown below. ...
Protein and proteome analytics
Protein and proteome analytics

... individual samples as well as individual steps during protein analysis will be provided according to the customers demands. ...
How to search the PDB
How to search the PDB

... hit (PDB entry 3NML in this example). This is followed by the remaining entries from the same family in order of model and data quality. ...
Tailor Made Protein Synthesis for HSCs - Barna Lab
Tailor Made Protein Synthesis for HSCs - Barna Lab

... of Pten has been shown to deplete HSCs and promote leukemogenesis. Strikingly, blocking increases in protein synthesis in a Pten-deficient background restores, to a large extent, normal HSC function and suppresses leukemogenesis. These findings suggest that a delicate balance in protein synthesis se ...
Slide 1
Slide 1

... Characteristics of the Ti-Plasmid Large circular plasmid containing genes for: Virulence Catabolism of specific opines ...
Proteins
Proteins

...  However, there are 20 amino acids, so at least 3 bases codes for one amino acid, since 42 = 16 and 43 = 64 ...
DO NOT OPEN UNTIL TOLD TO START
DO NOT OPEN UNTIL TOLD TO START

... D. yeast two-hybrid E. in situ hybridization 28. Technique that makes visible the locations where a certain mRNA is expressed E. in situ hybridization 29. This technique uses an antibody specific to a particular protein to purify all of the DNA sequences in a genome to which the protein is bound. B. ...
How to search the PDB
How to search the PDB

... hit (PDB entry 3NML in this example). This is followed by the remaining entries from the same family in order of model and data quality. ...
slides
slides

... proteases in blood clotting. Green portion: aa sequence is the same Notice the structural similarity and active site in red. Each cleaves between peptides ...
15-25 kD
15-25 kD

... – Class – Order – Family – Genus – Species • Traditional classification based upon traits: – Morphological – Behavioral ...
Document
Document

... MOLECULAR BIOLOGY TECHNIQUES III. analysis of gene expression ...
Document
Document

... MOLECULAR BIOLOGY TECHNIQUES III. analysis of gene expression ...
Monday - Biostatistics
Monday - Biostatistics

... Every cell in the human body contains the entire human genome: 3.3 Gb in which ~30K genes exist. The investigation of gene expression is meaningful because different cells, in different environments, doing different jobs express different genes. Cellular “Plans”: DNA - RNA - PROTEIN ...
< 1 ... 160 161 162 163 164 165 166 167 168 ... 221 >

Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
  • studyres.com © 2025
  • DMCA
  • Privacy
  • Terms
  • Report