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bIOCHEMISTRY
bIOCHEMISTRY

... joined by = bonds Found – plants & fish Liquids Healthier – “good fats” ...
Proteins - Westgate Mennonite Collegiate
Proteins - Westgate Mennonite Collegiate

... 2. the peptide bond joins the carboxyl group of one amino acid to the amino group of another; is formed by a condensation reaction 2. two amino acids fastened together by a peptide bond is called a dipeptide, several amino acids fastened together by peptide bonds are called a ...
• Will a base pair substitution, addition or deletion cause a
• Will a base pair substitution, addition or deletion cause a

... Retrieval of Genetic Information: Central to any information storage system is the ability to access and retrieve the information and to convert it to a usable form. In addition to the sequence information that will be translated into protein via the triplet code, a gene also contains sequence infor ...
Protein Powerpoint
Protein Powerpoint

... Act as messengers (hormones) ...
(1) Identify the secondary structure described in each of the
(1) Identify the secondary structure described in each of the

... stored in a freezer maintains its sweetness. What is the biochemical basis for this procedure? (11) Cell membranes contain proteins. Some of these proteins, involved in the transport of molecules across the membrane into the cell, span the entire membrane and are called trans-membrane proteins (or i ...
No Slide Title
No Slide Title

... Coiled-coil, three separate polypeptides called  chains are supertwisted Provide strength (stronger than ??) Connective tissue (tendons, cartilage, organic matrix of bone, cornea) ...
Gene Section FABP7 (fatty acid binding protein 7, brain)
Gene Section FABP7 (fatty acid binding protein 7, brain)

... brain injuries identified both these FABPs as more sensitive at detecting brain injury than markers currently in use for this purpose. Similarly, serum FABP7 and FABP3 served as markers for individuals who had undergone ischaemic stroke (Wunderlich et al., 2005). FABP7 levels were also elevated in t ...
PS 1 answers
PS 1 answers

... Where in a eukaryotic cell do you think you would find the following proteins residing? Be as specific as you can in terms of subcellular location. (a) an enzyme whose substrate is DNA The nucleus. DNA is found in the nucleus of eukaryotic cells, so an enzyme that acts on DNA would have to be found ...
Isolation of a cDNA for a nucleoside diphosphate kinase capable of
Isolation of a cDNA for a nucleoside diphosphate kinase capable of

... In higher plants, different types of self-recognition systems are operating during pollination to avoid self-fertilization. The selfrecognition signal is generated by the interaction between pollen ligand and stigma receptor proteins and transduced by a receptor kinase to induce incompatibility resp ...
SECTION D What Does DNA Do?
SECTION D What Does DNA Do?

... give structure and shape to living cells and that carry out all of the chemical reactions necessary for life. The importance of DNA is that it contains the information that is used to make all of the proteins on which life depends. The proteins whose structures are specified by our DNA do more than ...
“Beneficial” Metals and Microbes: Interactions involving
“Beneficial” Metals and Microbes: Interactions involving

... catalysis, redox centers, structure. ...
Biochemistry-lab-identifying
Biochemistry-lab-identifying

... protein molecular structure. Proteins can have different special shapes. Nucleic acid structures are smaller than the larger, sometimes floppy proteins. Often, proteins can bend, stretch, fold, spiral or form branches. Think about how hair can be straight, wavy, bouncy, curly, shiny and different co ...
Pipe-Cleaner Proteins
Pipe-Cleaner Proteins

... the order of colours is up to you (don’t use the order listed in step 1!), but only use each colour once. Be sure that the order you choose is NOT the same as your partners. Write out/colour the order below: Strand 1 Strand 2 3. Once coloured from end to end, what you have represents a chain of amin ...
Unit 1 PPT 2 (2bi-ii Protein structure)
Unit 1 PPT 2 (2bi-ii Protein structure)

... • The primary sequence of a protein is the order in which the amino acids are synthesised by translation into the polypeptide. ...
Exam1 Fall03
Exam1 Fall03

... compensate for the “thinner air” due to the oxygen pressures at higher elevations. This increased ventilation rate results in a reduction in the levels of CO2 dissolved in the blood. Which of the following accurately describes the effect of lowering the [CO2]dissolved on blood pH? a. It will result ...
Diapositiva 1 - UniFI
Diapositiva 1 - UniFI

... The protein is produced by expression from bacteria which are grown on minimal medium supplemented with small amounts of 15NH4Cl and 13C-labelled glucose as well as labelled and unlabelled amino acids. The idea is that only those amino acids which are added in labelled form become labelled in the pr ...
Class details
Class details

... OD = 2.5. [Protein] = ? ...
Secondary Structure Prediction Protein Folding
Secondary Structure Prediction Protein Folding

... • A new representation of protein secondary structure prediction based on frequent patterns by Birzele and Kramer from Germany Bioinformatics August 29 2006 • Use variable length patterns to define features for amino acids that can be used to predict secondary structures. • It is a window-less appro ...
c-MET - Siapec
c-MET - Siapec

...  nuclear regulatory protein that interacts with specific genes involved in cellular regulation, invasion and progression  expression of MTA-1 gene was increased four-times in cerbB2/neu stable transfectants cells compared to untransfected cells  MTA-1 is overexpressed in a variety of human cancer ...
How are the proteins built up
How are the proteins built up

... this loose coil can collapse into a sort of “molten globule” state, but this will be very different from the “molten globule” of proteins, because there is still only random structure in a random polymer, as opposed to a much more constrained, fairly unique three-dimensional structure of a protein. ...
Protein Structure
Protein Structure

... no longer have to be solvated by water – Cavities form that are complementary to any small molecules (or other large proteins)—binding sites/active sites (in the case of enzymes)----FUNCTION! ...
In silico Study of Target Proteins for Mycobacterium
In silico Study of Target Proteins for Mycobacterium

... understand physicochemical and structural properties of proteins and also to predict lead protein. Due to the drawbacks of experimental methods that have been used to characterize the proteins of various organisms are time consuming, costly and the fact that these methods not amendable to high throu ...
supplemental methods
supplemental methods

... build 124, 2005; pre-HapMap phase I data release) in the Scottish population prior to genotyping. Sequence was analysed using Sequencher 4.2 software (Gene Codes Corporation). Primer sets used to amplify GLI1 are available on request. Four multi-marker GLI1 tSNPs (r2≥0.8) were identified (rs3817474, ...
BITC1311 Intro to Biotechnology
BITC1311 Intro to Biotechnology

... a. What are some problems with the extraction of insulin from pig and cow carcasses? b. Describe how insulin is produced in pancreatic cells from preproinsulin precursor protein. c. Describe how recombinant insulin is produced from fusion proteins. ...
6.unknown-genes
6.unknown-genes

... If you can find functional domains, you may know something about the general behaviour of your protein, even if you don’t know exactly what its function is. But, as usual, be aware that non-significant matches are quite likely to be displayed in any analysis website – and at least look for some conf ...
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Expression vector

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for protein expression in cells. The vector is used to introduce a specific gene into a target cell, and can commandeer the cell's mechanism for protein synthesis to produce the protein encoded by the gene. Expression vectors are the basic tools in biotechnology for the production of proteins.The plasmid is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. The goal of a well-designed expression vector is the production of protein, and this may be achieve by the production of significant amount of stable messenger RNA, which can then be translated into protein. The protein may be expressed constitutively, or induced when necessary using an inducer. Escherichia coli is commonly used as the host for protein expression, other cell types however may also be used. An example of the use of expression vector is the production of insulin which is used for medical treatments of diabetes.
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