gWIZ GFP Mammalian Expression Vector
... the most widely used constitutive promoter for expressing high levels of trangene product in many mammalian cells and tissues. However, not all CMV IE gene promoter/enhancer-based expression vectors are created equal. Depending on the actual CMV IE gene sequences used and the context of the plasmid ...
... the most widely used constitutive promoter for expressing high levels of trangene product in many mammalian cells and tissues. However, not all CMV IE gene promoter/enhancer-based expression vectors are created equal. Depending on the actual CMV IE gene sequences used and the context of the plasmid ...
Hypothalamic Cell Line Maintenance
... cultures by retroviral transfer of SV40 T-Ag. The immortalized pituitary pool was sorted on a cell sorting flow cytometer based on GFP fluorescence as above. A cell line representing the heterogeneous population of POMC expressing pituitary neurons resulted: mPitA-POMC/GFP-1. This pituitary cell lin ...
... cultures by retroviral transfer of SV40 T-Ag. The immortalized pituitary pool was sorted on a cell sorting flow cytometer based on GFP fluorescence as above. A cell line representing the heterogeneous population of POMC expressing pituitary neurons resulted: mPitA-POMC/GFP-1. This pituitary cell lin ...
Supplementary Figure S1 a, Schematic diagrams of the
... Supplementary Figure S4 a, Analysis of mitosis progression, considering first stage: from prometaphase to late anaphase (chromosome segregation and beginning of cell division), and second stage: from late anaphase to completed cytokinesis and reattachment of daughter cells. These two periods were d ...
... Supplementary Figure S4 a, Analysis of mitosis progression, considering first stage: from prometaphase to late anaphase (chromosome segregation and beginning of cell division), and second stage: from late anaphase to completed cytokinesis and reattachment of daughter cells. These two periods were d ...
Brief description of pGLO
... and the “cloning” of “foreign” DNA in E. coli, attention turned to designing vectors that not only supported replication of foreign DNA sequences but which allowed expression of the genes they carried. This later type of cloning vector is referred to as an “expression vector”. To achieve expression ...
... and the “cloning” of “foreign” DNA in E. coli, attention turned to designing vectors that not only supported replication of foreign DNA sequences but which allowed expression of the genes they carried. This later type of cloning vector is referred to as an “expression vector”. To achieve expression ...
The UNC-112 gene in Caenorhabditis elegans Encodes a Novel
... are needed to see this picture. QuickTime™ and a ...
... are needed to see this picture. QuickTime™ and a ...
Methods, Part 2 - Rensselaer Polytechnic Institute
... • cDNA is frequently combined with a small circular piece of DNA, called a plasmid, before inserting it into a cell. The plasmid contains DNA sequences that help control when and where the cDNA gene will be expressed. ...
... • cDNA is frequently combined with a small circular piece of DNA, called a plasmid, before inserting it into a cell. The plasmid contains DNA sequences that help control when and where the cDNA gene will be expressed. ...
1) digest DNA inserts with restriction enzyme(s).
... Comes from a jellyfish, Aequorea victoria Gene has been cloned and transferred into a wide variety of “heterologous” expression systems … including Drosophila, mammalian cells, C. elegans, yeast, zebrafish etc. etc. **** Permits dynamic and in vivo analysis**** of biological processes ...
... Comes from a jellyfish, Aequorea victoria Gene has been cloned and transferred into a wide variety of “heterologous” expression systems … including Drosophila, mammalian cells, C. elegans, yeast, zebrafish etc. etc. **** Permits dynamic and in vivo analysis**** of biological processes ...
embj201490361-sup-0002
... constructs for 6xHis-Ub and WT or mutant TRIM5αhu-HA. After 48 hr, 6xHis-Ub was pulled down (PD) with a Ni2+ affinity matrix, followed by analysis of the input and affinity purified samples by IB with antibodies detecting the HA-tag (HA) or βactin (loading control). All blots are representative of a ...
... constructs for 6xHis-Ub and WT or mutant TRIM5αhu-HA. After 48 hr, 6xHis-Ub was pulled down (PD) with a Ni2+ affinity matrix, followed by analysis of the input and affinity purified samples by IB with antibodies detecting the HA-tag (HA) or βactin (loading control). All blots are representative of a ...
A549/GFP Cell Line - Cell Biolabs, Inc.
... Liquid nitrogen Note: For best results begin culture of cells immediately upon receipt. If this is not possible, store at -80ºC until first culture. Store subsequent cultured cells long term in liquid nitrogen. ...
... Liquid nitrogen Note: For best results begin culture of cells immediately upon receipt. If this is not possible, store at -80ºC until first culture. Store subsequent cultured cells long term in liquid nitrogen. ...
Recent advances in technology for measuring and manipulating cell
... Over the last several years, both random and semirational mutagenesis have produced GFP variants with new colors, improved folding, extinction coefficient (the strength of light absorption per molecule), quantum yield (the proportion of absorbed light that ends up as fluorescence), and/or altered pH ...
... Over the last several years, both random and semirational mutagenesis have produced GFP variants with new colors, improved folding, extinction coefficient (the strength of light absorption per molecule), quantum yield (the proportion of absorbed light that ends up as fluorescence), and/or altered pH ...
Expression of the Vacuolar Iron Transporter
... Iron deficiency is a nutritional problem in plants and reduces crop productivity and quality. We have investigated the function of a small family of five membrane proteins that have amino acid sequence homology to AtVIT1 and consequently also to yeast CCC1p (1-3). These Vacuolar-Iron-Transporterlike ...
... Iron deficiency is a nutritional problem in plants and reduces crop productivity and quality. We have investigated the function of a small family of five membrane proteins that have amino acid sequence homology to AtVIT1 and consequently also to yeast CCC1p (1-3). These Vacuolar-Iron-Transporterlike ...
Next-Generation Approaches to Overcome the Challenges of
... 3) Construct plasmid containing HMF pathway, insert into genome 4) Test the function of dCas9 by knocking out RFP and test the function of HMF pathway by doing a furfural consumption assay 5) Assemble CRISPR RNA library and insert into plasmid 6) Conduct gene knockdown experiments using combinatoria ...
... 3) Construct plasmid containing HMF pathway, insert into genome 4) Test the function of dCas9 by knocking out RFP and test the function of HMF pathway by doing a furfural consumption assay 5) Assemble CRISPR RNA library and insert into plasmid 6) Conduct gene knockdown experiments using combinatoria ...
Classification of Enteric Progenitors in Zebrafish During Development
... • Composed of different subtypes of neurons and glial cells ...
... • Composed of different subtypes of neurons and glial cells ...
Table S1: Transgenic zebrafish strains used in this study Transgenic
... green fluorescent protein 1. Xenopus catenin with 4 point mutations; ...
... green fluorescent protein 1. Xenopus catenin with 4 point mutations; ...
Synthetic Biology: Gene Transformation and Protein Purification
... Vector • The vector is the method used for inserting genes into a cell during transformation. ...
... Vector • The vector is the method used for inserting genes into a cell during transformation. ...
pGLO
... from the jellyfish, Aequorea victoria • The pGLO plasmid contains several genes that are necessary for producing and expressing the pGLO protein in whatever organism it is found in. ...
... from the jellyfish, Aequorea victoria • The pGLO plasmid contains several genes that are necessary for producing and expressing the pGLO protein in whatever organism it is found in. ...
pGLO - Blue Valley Schools
... from the jellyfish, Aequorea victoria • The pGLO plasmid contains several genes that are necessary for producing and expressing the pGLO protein in whatever organism it is found in. ...
... from the jellyfish, Aequorea victoria • The pGLO plasmid contains several genes that are necessary for producing and expressing the pGLO protein in whatever organism it is found in. ...
Fluorescent Dyes and Proteins
... in response to light, and changes conformation, but opens a Na+ channel. This allows light to control membrane voltage and trigger neuron firing. ...
... in response to light, and changes conformation, but opens a Na+ channel. This allows light to control membrane voltage and trigger neuron firing. ...
glofish - Science Centre
... Yahoo! Answer (Open question titled “GFP”, posted by MileyFan) I’d just come back from the aquarium today, and was overwhelmed with the sights of numerous fishes, including the agile sword-tails, the fierce angel-fishes and the graceful koi. However, I was most impressed with a particular zebrafish ...
... Yahoo! Answer (Open question titled “GFP”, posted by MileyFan) I’d just come back from the aquarium today, and was overwhelmed with the sights of numerous fishes, including the agile sword-tails, the fierce angel-fishes and the graceful koi. However, I was most impressed with a particular zebrafish ...
Identification of Mucin 2 as a Strong Promoter for Gut
... in a human intestinal epithelial cell line (Caco-2 cells) and confirmed to exhibit green fluorescence in these gut cells. A microneedle and injector was used to inject the lentiviral particles, containing the target gene constructs, into the subgerminal cavity of the blastodisc. Fifteen chimeric fou ...
... in a human intestinal epithelial cell line (Caco-2 cells) and confirmed to exhibit green fluorescence in these gut cells. A microneedle and injector was used to inject the lentiviral particles, containing the target gene constructs, into the subgerminal cavity of the blastodisc. Fifteen chimeric fou ...
ara Operon
... • Study of biological processes (example: synthesis of proteins) • Localization and regulation of gene expression • Cell movement • Cell fate during development ...
... • Study of biological processes (example: synthesis of proteins) • Localization and regulation of gene expression • Cell movement • Cell fate during development ...
Presentation - Harlem Children Society
... • We are trying to locate the genes responsible for the formation of the GPI proteins during cell wall development so that a drug can be made targeting every gene responsible in the creation of the cell wall, killing the fungi, Candida albicans. • However, Candida albicans is unsafe to work with bec ...
... • We are trying to locate the genes responsible for the formation of the GPI proteins during cell wall development so that a drug can be made targeting every gene responsible in the creation of the cell wall, killing the fungi, Candida albicans. • However, Candida albicans is unsafe to work with bec ...
DAW Lecture 3 PPT
... hours post transfection Stable: Integration of the transfected DNA into the cell genome - selectable marker like neomycin resistance required “stably transfected” cell line ...
... hours post transfection Stable: Integration of the transfected DNA into the cell genome - selectable marker like neomycin resistance required “stably transfected” cell line ...
Molecular size scales in biology
... 3. Lac repressor complex with DNA 4. Hemoglobin 5. Myoglobin 6. Green fluorescent protein (GFP) 7. RNA polymerase • Download a program that allows structure viewing such as RasMol (U. Massachusettes). • Use the measurement tools in these programs to measure the maximal length of these molecules (max ...
... 3. Lac repressor complex with DNA 4. Hemoglobin 5. Myoglobin 6. Green fluorescent protein (GFP) 7. RNA polymerase • Download a program that allows structure viewing such as RasMol (U. Massachusettes). • Use the measurement tools in these programs to measure the maximal length of these molecules (max ...
Green fluorescent protein
The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm.In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. In modified forms it has been used to make biosensors, and many animals have been created that express GFP as a proof-of-concept that a gene can be expressed throughout a given organism. The GFP gene can be introduced into organisms and maintained in their genome through breeding, injection with a viral vector, or cell transformation. To date, the GFP gene has been introduced and expressed in many Bacteria, Yeast and other Fungi, fish (such as zebrafish), plant, fly, and mammalian cells, including human. Martin Chalfie, Osamu Shimomura, and Roger Y. Tsien were awarded the 2008 Nobel Prize in Chemistry on 10 October 2008 for their discovery and development of the green fluorescent protein.