Make:targeting signals
... Calmodulin (CaM) : An abundant 149 amino acid, highly conserved cytoplasmic protein with 4 binding sites for Ca2+ each formed by "EF-hands." Many other homologous Ca2+ binding proteins of this large EF-hand family act as Ca switches and Ca buffers. The Ca2+ ions bind cooperatively and become encircl ...
... Calmodulin (CaM) : An abundant 149 amino acid, highly conserved cytoplasmic protein with 4 binding sites for Ca2+ each formed by "EF-hands." Many other homologous Ca2+ binding proteins of this large EF-hand family act as Ca switches and Ca buffers. The Ca2+ ions bind cooperatively and become encircl ...
Using virus-induced gene silencing to identify novel regulators of
... In addition, the effect of silencing each gene on the plasmodesmal function was measured by bombarding each plant with a DNA construct for expression of green fluorescent protein (GFP) and then monitoring the spread of GFP to adjacent cells. In this study, YY genes were silenced in VV plants. KK pla ...
... In addition, the effect of silencing each gene on the plasmodesmal function was measured by bombarding each plant with a DNA construct for expression of green fluorescent protein (GFP) and then monitoring the spread of GFP to adjacent cells. In this study, YY genes were silenced in VV plants. KK pla ...
Restriction Digestion and Analysis of Lambda DNA
... digestive enzymes involved in the breakdown of arabinose are clustered together in what is known as the arabinose operon. Transcription of these three genes requires the simultaneous presence of the DNA template (promoter and operon), RNA polymerase, a DNA binding proteins called araC and arabinose. ...
... digestive enzymes involved in the breakdown of arabinose are clustered together in what is known as the arabinose operon. Transcription of these three genes requires the simultaneous presence of the DNA template (promoter and operon), RNA polymerase, a DNA binding proteins called araC and arabinose. ...
Supplement
... of DNA at 1µg. µl-1 was injected into the coelomic cavity of stage 13 embryos and 3 pulses of 5ms at 50V were applied using homemade electrodes. RhoA expression vector was kindly provided by Dr. Sheng (4); Fgf10 was cloned from a limb cDNA library and subcloned into a pCAG-ires-GFP construct. Mouse ...
... of DNA at 1µg. µl-1 was injected into the coelomic cavity of stage 13 embryos and 3 pulses of 5ms at 50V were applied using homemade electrodes. RhoA expression vector was kindly provided by Dr. Sheng (4); Fgf10 was cloned from a limb cDNA library and subcloned into a pCAG-ires-GFP construct. Mouse ...
Transformation and Protein Purification
... Arabinose controls expression of GFP gene: Transfer Bacteria ...
... Arabinose controls expression of GFP gene: Transfer Bacteria ...
Doxycycline Concentration (ng/µl) Median Intensity
... of the perfect bell curve is that the median and mean values are the same. By comparing those values here, one can determine how well the observed induction of GFP approximates an ideal induction. For each concentration, the results of nine clonal lines are shown (average ± S.D.). ...
... of the perfect bell curve is that the median and mean values are the same. By comparing those values here, one can determine how well the observed induction of GFP approximates an ideal induction. For each concentration, the results of nine clonal lines are shown (average ± S.D.). ...
Site directed mutagenesis as an efficient way to enhance structural
... 1998). The coexistence of neutral and anion chromophores is responsible for these two excitation peaks and it has just few advantages and many disadvantages for applications in developmental and cell biology (Tsien, 1998). In order to make this protein better suit as a maker of gene expression or pr ...
... 1998). The coexistence of neutral and anion chromophores is responsible for these two excitation peaks and it has just few advantages and many disadvantages for applications in developmental and cell biology (Tsien, 1998). In order to make this protein better suit as a maker of gene expression or pr ...
Transformation and Protein Purification
... Arabinose controls expression of GFP gene: Transfer Bacteria ...
... Arabinose controls expression of GFP gene: Transfer Bacteria ...
LAB prep for Transformation using pGLO
... Arabinose controls expression of GFP gene: Transfer Bacteria ...
... Arabinose controls expression of GFP gene: Transfer Bacteria ...
Laura Bassi Centres of Expertise - PlantBioP Plant
... the biological impact of specific N-glycan profiles is largely unknown, many studies have demonstrated that certain N-glycan residues significantly enhance therapeutic potency. Consequently glycosylation is playing an increasingly important role in drug development. The expression systems in current ...
... the biological impact of specific N-glycan profiles is largely unknown, many studies have demonstrated that certain N-glycan residues significantly enhance therapeutic potency. Consequently glycosylation is playing an increasingly important role in drug development. The expression systems in current ...
Bioluminescence - Fat Tuesday Productions
... fuses to β-arrestin and emits blue light with an emission peak at 395 nm. If the β-arrestin is ubiquitinated, a transfer of energy will occur between Rluc and the GFP2 fused to the N terminus of ubiquitin, resulting in re-emission of light with an emission peak at 510 nm. ...
... fuses to β-arrestin and emits blue light with an emission peak at 395 nm. If the β-arrestin is ubiquitinated, a transfer of energy will occur between Rluc and the GFP2 fused to the N terminus of ubiquitin, resulting in re-emission of light with an emission peak at 510 nm. ...
gfp - Ana Maria Caputo
... effective. The band for the control was also this length, but it was much thinner than that of the replicated band. There should have been no band present run in the control because no DNA was introduced to that culture at all. Perhaps the sample used had a few gfp plasmids present before the experi ...
... effective. The band for the control was also this length, but it was much thinner than that of the replicated band. There should have been no band present run in the control because no DNA was introduced to that culture at all. Perhaps the sample used had a few gfp plasmids present before the experi ...
... dynamics, is limited by the fluorescent probes. Thus, we take advantage of the localized surface plasmon resonances that result from the interaction of light with small metal nanoparticles to improve the brightness and photostability of nearby fluorescent labels. We have measured the fundamental pro ...
Attachment 3 Speakers（English version）
... Research interests of the Yang group include synthetic organic chemistry, bioorganic chemistry, and chemical biology. The group developed a mild and general method for epoxidation of olefins using dioxiranes generated in situ from ketones and Oxone. They also developed chiral ketone catalysts for hi ...
... Research interests of the Yang group include synthetic organic chemistry, bioorganic chemistry, and chemical biology. The group developed a mild and general method for epoxidation of olefins using dioxiranes generated in situ from ketones and Oxone. They also developed chiral ketone catalysts for hi ...
Optical tweezers - Niels Bohr Institutet
... Literature very sparse, only one value reported for Qdots with radius=2nm: a ~ 104 Å3. Jauffred, Richardson, Oddershede, Nano Letters vol.8 p.3376 (2008). ...
... Literature very sparse, only one value reported for Qdots with radius=2nm: a ~ 104 Å3. Jauffred, Richardson, Oddershede, Nano Letters vol.8 p.3376 (2008). ...
Lecture 10/09
... stabilize actin filaments by inhibiting depolymerization. Phalloidins and phallacidins are similar peptides, used more or less interchangeably to label filamentous actin. Wheat germ agglutinin (WGA), a lectin that binds sialic acid - an important constituent of glycoproteins and glycolipids. Green F ...
... stabilize actin filaments by inhibiting depolymerization. Phalloidins and phallacidins are similar peptides, used more or less interchangeably to label filamentous actin. Wheat germ agglutinin (WGA), a lectin that binds sialic acid - an important constituent of glycoproteins and glycolipids. Green F ...
Highligh in Physics 2005
... The study of phenomena taking place in proteins that can only be described by quantum mechanics is particularly complicated, due to the large size of the system and the lack of symmetries. In these cases, a possible approach is to describe quantum-mechanically only a part of the whole protein, accou ...
... The study of phenomena taking place in proteins that can only be described by quantum mechanics is particularly complicated, due to the large size of the system and the lack of symmetries. In these cases, a possible approach is to describe quantum-mechanically only a part of the whole protein, accou ...
Laboratory Exercise #7: Column Chromatography of GFP proteins
... (GFP) from the jellyfish Aequoria victoria. As the bacterial cells express proteins from their endogenous genome, they will also express the GFP protein from the GFP gene within the pGLO plasmid. You have observed this already. When bacterial cells are grown in the presence of the sugar arabinose (t ...
... (GFP) from the jellyfish Aequoria victoria. As the bacterial cells express proteins from their endogenous genome, they will also express the GFP protein from the GFP gene within the pGLO plasmid. You have observed this already. When bacterial cells are grown in the presence of the sugar arabinose (t ...
Study Guide
... changing conditions. A classic example concerns the so-called "lac operon" in the bacterium E. coli. The lac operon is a cluster of three genes for proteins involved in digesting lactose, a sugar that E. coli can use as food. When lactose is the only source of carbon in E. coli's environment, these ...
... changing conditions. A classic example concerns the so-called "lac operon" in the bacterium E. coli. The lac operon is a cluster of three genes for proteins involved in digesting lactose, a sugar that E. coli can use as food. When lactose is the only source of carbon in E. coli's environment, these ...
Trekking along the Cytoskeleton
... tween a variety of organelles will be imaged concurrently. Green Golgi will be visualized moving over red actin and blue ER while secreting yellow fluorescent protein. The interactions of proteins involved in the molecular machinery of such organelle movement or even in vesicle budding and fusion wi ...
... tween a variety of organelles will be imaged concurrently. Green Golgi will be visualized moving over red actin and blue ER while secreting yellow fluorescent protein. The interactions of proteins involved in the molecular machinery of such organelle movement or even in vesicle budding and fusion wi ...
PHENOTYPIC CHANGES IN IRRADIATED ENDOTHELIAL CELLS
... The exposure of the abdomino-pelvic region to ionizing radiation is associated with untoward healthy digestive tissue injury. Radiation fibrosis is characterized by an excessive and uncontrolled synthesis of extracellular matrix by mesenchymal cells. The endothelial to mesenchymal transition (EndoMT ...
... The exposure of the abdomino-pelvic region to ionizing radiation is associated with untoward healthy digestive tissue injury. Radiation fibrosis is characterized by an excessive and uncontrolled synthesis of extracellular matrix by mesenchymal cells. The endothelial to mesenchymal transition (EndoMT ...
Document
... Leipzig, 04103 Leipzig, Germany Recent growing evidence suggests the existence of G-protein coupled receptors as dimers or even higher order oligomers. In contrast to indirect biochemical and physiological experiments, fluorescence resonance energy transfer (FRET) allows the direct and noninvasive v ...
... Leipzig, 04103 Leipzig, Germany Recent growing evidence suggests the existence of G-protein coupled receptors as dimers or even higher order oligomers. In contrast to indirect biochemical and physiological experiments, fluorescence resonance energy transfer (FRET) allows the direct and noninvasive v ...
Imaging of vascular gene expression is an important part of
... level of transgene expression for efficient therapeutic effect on the targets; and (c) the functional period of therapeutic genes at the targets. Optical imaging, which is based on the detection of fluorescence or emitted light from the luciferase-luciferin reaction, has shown some prominent advanta ...
... level of transgene expression for efficient therapeutic effect on the targets; and (c) the functional period of therapeutic genes at the targets. Optical imaging, which is based on the detection of fluorescence or emitted light from the luciferase-luciferin reaction, has shown some prominent advanta ...
CELL-FREE SYSTEMS FOR STUDYING THE COMPONENTS AND
... cells is to construct fusion proteins, in which green fluorescent protein (GFP) is attached by genetic engineering techniques to the protein of interest. When a cDNA encoding such a fusion protein is expressed in a cell, the protein is readily visible in a fluorescent microscope, so that it can be f ...
... cells is to construct fusion proteins, in which green fluorescent protein (GFP) is attached by genetic engineering techniques to the protein of interest. When a cDNA encoding such a fusion protein is expressed in a cell, the protein is readily visible in a fluorescent microscope, so that it can be f ...
Green fluorescent protein
The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm.In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. In modified forms it has been used to make biosensors, and many animals have been created that express GFP as a proof-of-concept that a gene can be expressed throughout a given organism. The GFP gene can be introduced into organisms and maintained in their genome through breeding, injection with a viral vector, or cell transformation. To date, the GFP gene has been introduced and expressed in many Bacteria, Yeast and other Fungi, fish (such as zebrafish), plant, fly, and mammalian cells, including human. Martin Chalfie, Osamu Shimomura, and Roger Y. Tsien were awarded the 2008 Nobel Prize in Chemistry on 10 October 2008 for their discovery and development of the green fluorescent protein.