Modeling a -Sheet of Green Fluorescent Protein
... of the β-sheet is predominantly not charged (gray amino acid sidechains) and the other side is mostly charged (red and blue in the amino acid sidechains). This would suggest that the hydrophobic (non-charged) side of the β-sheet might be facing inside the GFP protein barrel and the charged side of t ...
... of the β-sheet is predominantly not charged (gray amino acid sidechains) and the other side is mostly charged (red and blue in the amino acid sidechains). This would suggest that the hydrophobic (non-charged) side of the β-sheet might be facing inside the GFP protein barrel and the charged side of t ...
pGLO
... pGLO Plasmid: Most Important Components • bla gene – Bacteria with this gene produce beta lactamase, an enzyme that allows them to grow in the presence of ampicillin • GFP gene – Bacteria with this gene glow under UV light ...
... pGLO Plasmid: Most Important Components • bla gene – Bacteria with this gene produce beta lactamase, an enzyme that allows them to grow in the presence of ampicillin • GFP gene – Bacteria with this gene glow under UV light ...
Evaluation and Comparison of the GUS, LUC and GFP Reporter
... Whether reporter activity is detectable within a plant cell or tissue depends on the promoter strength, duration of promoter activity, stability of the reporter mRNA, stability and activity of the reporter protein, the intrinsic background level within a tissue, and reporter signal detection techniq ...
... Whether reporter activity is detectable within a plant cell or tissue depends on the promoter strength, duration of promoter activity, stability of the reporter mRNA, stability and activity of the reporter protein, the intrinsic background level within a tissue, and reporter signal detection techniq ...
24.8 brief comms MH - Department of Entomology
... We monitored the timing of kin recognition by using fluorescence microscopy (Fig. 1b–e). Different clones mixed during early aggregation (Fig. 1b) but separated when they emerged as slugs (Fig. 1c); however, single-clone controls remained mixed under the same conditions (Fig. 1d, e). These results i ...
... We monitored the timing of kin recognition by using fluorescence microscopy (Fig. 1b–e). Different clones mixed during early aggregation (Fig. 1b) but separated when they emerged as slugs (Fig. 1c); however, single-clone controls remained mixed under the same conditions (Fig. 1d, e). These results i ...
Unique Protein Reporter Assays: Green Fluorescent Proteins (GFP
... Fluorescent reporter proteins are valuable non-invasive molecular tools for in vivo real-time imaging of living cells and tissues as well as in vitro fluorescence labeling. A major drawback of existing GFP-like reporter proteins is their strict requirement for molecular oxygen as a cofactor for the ...
... Fluorescent reporter proteins are valuable non-invasive molecular tools for in vivo real-time imaging of living cells and tissues as well as in vitro fluorescence labeling. A major drawback of existing GFP-like reporter proteins is their strict requirement for molecular oxygen as a cofactor for the ...
Chapter 8-1
... • Each a subunit has 2 half-channels that are physically separated (offset) from one another • One half-channel leads from intermembrane (cytosolic) space into the middle of the a subunit; the other leads from the middle of the a subunit into the matrix • Each proton moves from the intermembrane spa ...
... • Each a subunit has 2 half-channels that are physically separated (offset) from one another • One half-channel leads from intermembrane (cytosolic) space into the middle of the a subunit; the other leads from the middle of the a subunit into the matrix • Each proton moves from the intermembrane spa ...
Reporter genes
... The human growth hormone (hGH) encoded reporter protein is secreted into the culture medium by transfected cells. The hGH from the supernatant of the culture medium binds to the antibody on the plate. Subsequently, the bound hGH is detected in two steps via a digoxigenincoupled anti-hGH antibody and ...
... The human growth hormone (hGH) encoded reporter protein is secreted into the culture medium by transfected cells. The hGH from the supernatant of the culture medium binds to the antibody on the plate. Subsequently, the bound hGH is detected in two steps via a digoxigenincoupled anti-hGH antibody and ...
GFP
... The human growth hormone (hGH) encoded reporter protein is secreted into the culture medium by transfected cells. The hGH from the supernatant of the culture medium binds to the antibody on the plate. Subsequently, the bound hGH is detected in two steps via a digoxigenincoupled anti-hGH antibody and ...
... The human growth hormone (hGH) encoded reporter protein is secreted into the culture medium by transfected cells. The hGH from the supernatant of the culture medium binds to the antibody on the plate. Subsequently, the bound hGH is detected in two steps via a digoxigenincoupled anti-hGH antibody and ...
Physikalisch-Chemisches Kolloquium am Montag, den 11.01.2016 um 17:00 (AR-F002)
... microscopes, by switching fluorescent molecules between an emitting (“on”) and a non-emitting (“off”) state. Here, we introduce a novel general concept, which makes use of multiple off-state transitions (MOST) for coordinate-targeted fluorescence nanoscopy. We realize the concept in the form of Prot ...
... microscopes, by switching fluorescent molecules between an emitting (“on”) and a non-emitting (“off”) state. Here, we introduce a novel general concept, which makes use of multiple off-state transitions (MOST) for coordinate-targeted fluorescence nanoscopy. We realize the concept in the form of Prot ...
RDM Day One Interpretation Questions 1.
... Include the total size of the plasmid, the size of each fragment, and the location and identity of each restriction enzyme cut site. ...
... Include the total size of the plasmid, the size of each fragment, and the location and identity of each restriction enzyme cut site. ...
Name: TF Name:
... and paste the sequence of the protein into the search field that says “Or you can paste your own sequence in the box below.” Click on “Compute parameters.” v) Record the “molecular weight” of GFP. This is the molar mass that will determine how far your GFP protein will migrate through the polyacryla ...
... and paste the sequence of the protein into the search field that says “Or you can paste your own sequence in the box below.” Click on “Compute parameters.” v) Record the “molecular weight” of GFP. This is the molar mass that will determine how far your GFP protein will migrate through the polyacryla ...
Supplementary material Recruitment of a myosin
... We fused GFP to the amino-terminus of the MHCK A coding region by using the vector pDXA-GFP2 [S1]. An EcoRI site was engineered by PCR at codon 2 of the MHCK A coding region. This allowed the entire MHCK A gene to be inserted at the corresponding site of pDXA-GFP2. The resultant construct relied on ...
... We fused GFP to the amino-terminus of the MHCK A coding region by using the vector pDXA-GFP2 [S1]. An EcoRI site was engineered by PCR at codon 2 of the MHCK A coding region. This allowed the entire MHCK A gene to be inserted at the corresponding site of pDXA-GFP2. The resultant construct relied on ...
Green Fluorescent Protein
... frequently used and requires less time) 3. Sonication will break open membranes in an efficient way. ...
... frequently used and requires less time) 3. Sonication will break open membranes in an efficient way. ...
Role of cystinosin in vesicular trafficking and membrane fusion
... structures and a diminution of the usual pattern of small discrete intracytoplasmic vesicles characteristic of lysosomes. The number of these structures was drastically decreased when cystinosin C-terminal tail, its 5th inter-TM loop, or both motifs were altered. The enlarged lysosomes are reminisce ...
... structures and a diminution of the usual pattern of small discrete intracytoplasmic vesicles characteristic of lysosomes. The number of these structures was drastically decreased when cystinosin C-terminal tail, its 5th inter-TM loop, or both motifs were altered. The enlarged lysosomes are reminisce ...
pNZ:vig Vector information: IRES
... Suitable host strain: NZ9000 and other NICE system vectors derivatives ...
... Suitable host strain: NZ9000 and other NICE system vectors derivatives ...
Cell Lysis Protocols for the Protein Extraction Station
... Cell Lysis Protocols for the Protein Extraction Station Overview: Many biotechnology labs around the world use bacteria to produce large quantities of a specific DNA or protein. The bacteria act as factories to replicate the DNA and produce a specific protein. Often the protein of interest is trappe ...
... Cell Lysis Protocols for the Protein Extraction Station Overview: Many biotechnology labs around the world use bacteria to produce large quantities of a specific DNA or protein. The bacteria act as factories to replicate the DNA and produce a specific protein. Often the protein of interest is trappe ...
Obtaining information from a cloned gene
... Western blot analysis can detect one protein in a mixture of any number of proteins, while giving information about the size of the protein. This method requires the use of a high-quality antibody directed against a desired protein. ...
... Western blot analysis can detect one protein in a mixture of any number of proteins, while giving information about the size of the protein. This method requires the use of a high-quality antibody directed against a desired protein. ...
Spectroscopy of Proteins
... • The final product of the genes, translated form genes (mutation in gene leads to a mutated protein) • Made of a verity of 20 amino acid building blocks • Exert all the biological functions of the organism: enzymes, antibodies, cytoskeletons, hormones, receptors ...
... • The final product of the genes, translated form genes (mutation in gene leads to a mutated protein) • Made of a verity of 20 amino acid building blocks • Exert all the biological functions of the organism: enzymes, antibodies, cytoskeletons, hormones, receptors ...
Confocal Fluorescence Microscopy
... A confocal fluorescence microscope is a serial rather than parallel imageacquisition device: the object is illuminated point by point and the generated fluorescence, imaged onto the detection pinhole, is measured sequentially for each illuminated point. In such an instrument, the image acquisition is ...
... A confocal fluorescence microscope is a serial rather than parallel imageacquisition device: the object is illuminated point by point and the generated fluorescence, imaged onto the detection pinhole, is measured sequentially for each illuminated point. In such an instrument, the image acquisition is ...
+ Glyphosate X
... GFP glows bright green when irradiated by blue or UV light This is a non destructive assay so the same cells can be monitored all the way through It fluoresces green under UV illumination It has been used for selection on its own ...
... GFP glows bright green when irradiated by blue or UV light This is a non destructive assay so the same cells can be monitored all the way through It fluoresces green under UV illumination It has been used for selection on its own ...
Slide 1
... Storage: The clone should be stored at -20 0C. This clone shipped at ambient temperature ...
... Storage: The clone should be stored at -20 0C. This clone shipped at ambient temperature ...
NIH3T3/GFP Cell Line
... Note: For best results begin culture of cells immediately upon receipt. If this is not possible, store at -80ºC until first culture. Store subsequent cultured cells long term in liquid nitrogen. ...
... Note: For best results begin culture of cells immediately upon receipt. If this is not possible, store at -80ºC until first culture. Store subsequent cultured cells long term in liquid nitrogen. ...
Looking for a strong promoter for Physcomitrella
... The 35S promoter of the cauliflower mosaic virus is a strong and constitutive promoter that is widely used in plant systems. However, in the moss Physcomitrella patens, its promoter strength is weak or all but silent in the dark.1 Therefore, it is necessary to find promoters that show higher express ...
... The 35S promoter of the cauliflower mosaic virus is a strong and constitutive promoter that is widely used in plant systems. However, in the moss Physcomitrella patens, its promoter strength is weak or all but silent in the dark.1 Therefore, it is necessary to find promoters that show higher express ...
Development of Escherichia coli systemic infection model
... drug and they are genetically identical to the drug-susceptible cell. Their existence has been shown in several in vitro studies, but their emergence during in vivo infections is less reported. Uropathogenic Escherichia coli (UPEC) is a main cause of urinary tract infections, but it is also able to ...
... drug and they are genetically identical to the drug-susceptible cell. Their existence has been shown in several in vitro studies, but their emergence during in vivo infections is less reported. Uropathogenic Escherichia coli (UPEC) is a main cause of urinary tract infections, but it is also able to ...
PGLO Transformation LAB AP LAB 7
... Often carry genes for antibiotic resistance Can be passed from one bacterium to another ...
... Often carry genes for antibiotic resistance Can be passed from one bacterium to another ...
Green fluorescent protein
The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm.In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. In modified forms it has been used to make biosensors, and many animals have been created that express GFP as a proof-of-concept that a gene can be expressed throughout a given organism. The GFP gene can be introduced into organisms and maintained in their genome through breeding, injection with a viral vector, or cell transformation. To date, the GFP gene has been introduced and expressed in many Bacteria, Yeast and other Fungi, fish (such as zebrafish), plant, fly, and mammalian cells, including human. Martin Chalfie, Osamu Shimomura, and Roger Y. Tsien were awarded the 2008 Nobel Prize in Chemistry on 10 October 2008 for their discovery and development of the green fluorescent protein.