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Accompanying protein alterations in malignant cells with a
Accompanying protein alterations in malignant cells with a

... polymerization both in intact cells and in vitro [3,27,28]. A mutant p53 codon homozygous substitution was detected for PTX22, whereas for PTX10 and EPOA-R both a wildtype allele and a mutant p53 codon were found [(⫹/⫺) Table 1] [28]. For PTX10, little wild-type p53 function was detected, which is c ...
Networks Inferred from Biochemical Data Reveal Profound
Networks Inferred from Biochemical Data Reveal Profound

... complementary approach in which we started with “functional” data characterizing the responses of cells to biological ligands and small molecule drugs and then used inference methods to assemble a network. We exposed primary human hepatocytes and HepG2 liver cancer cells to one of seven ...
Megakaryoblastic Leukemia-1/2, a
Megakaryoblastic Leukemia-1/2, a

... muscle-specific genes during differentiation. Microinjection of anti-SRF antibodies blocked the serum induction of the c-fos gene in NIH3T3 cells as well as the expression of musclespecific genes and the terminal differentiation of C2C12 skeletal myoblasts (9, 10). Skeletal muscle differentiation in ...
C9orf72 binds SMCR8, localizes to lysosomes and regulates
C9orf72 binds SMCR8, localizes to lysosomes and regulates

... Loewith, 2015; Kim et al., 2002), altered mTORC1 signaling could also contribute to the increased size of SMCR8 KO cells. We therefore measured mTORC1 signaling under basal cell growth conditions by assessing the phosphorylation state of ribosomal protein S6 (S235/S236 phosphorylation sites), a down ...
Platelet releasate increases the proliferation and
Platelet releasate increases the proliferation and

... regarding the actions of platelets and platelet-released products on osteogenic cells. We show, herein, that releasate from thrombin-activated platelets increases the migration and proliferation of osteogenic cultures of bone marrow cells. Using a scratch wound assay, we demonstrated that platelet r ...
POTASSIUM BALANCE
POTASSIUM BALANCE

... The tubular defects in sodium chloride transport are almost identical to that seen with chronic ingestion of a loop diuretic (mimicking Bartter syndrome) or a thiazide diuretic (mimicking Gitelman syndrome). – Impaired sodium chloride reabsorption leads to mild volume depletion and activation of the ...
Condition-Dependent Cell Volume and Concentration of
Condition-Dependent Cell Volume and Concentration of

... cellular copy numbers, the total cell volume and number of cells in a sample need to be known. Unfortunately, even for the often studied model bacterium Escherichia coli this information is hardly available and furthermore, certain measures (e.g. cell volume) are also dependent on the growth conditi ...
An emerging role for prdm family genes in dorsoventral patterning of
An emerging role for prdm family genes in dorsoventral patterning of

Poor Devils: The Plight of the Tasmanian Devils
Poor Devils: The Plight of the Tasmanian Devils

1 Collaborative Control of Cell Cycle Progression by the
1 Collaborative Control of Cell Cycle Progression by the

... cell division. In cycling Xenopus embryo extracts, pharmacological inhibition of PP1 activity triggers premature mitotic entry (Walker et al. 1992). In multiple myeloma, a malignancy of plasma B cells, whole genome sequencing approaches uncover dis3 as one of the most frequently mutated genes in thi ...
Life under pressure: hydrostatic pressure in cell growth and function
Life under pressure: hydrostatic pressure in cell growth and function

... [16,17]. MS channels arose early in evolution and are present across all three domains of Archaea, Bacteria and Eukarya. Indeed, pressure-induced activation of MS channels is postulated to be one of the first signal transduction cascades that arose with the onset of cellular life [16,17]. In plants, ...
OPEN STOMATA1 opens the door to ABA signaling in Arabidopsis
OPEN STOMATA1 opens the door to ABA signaling in Arabidopsis

... what extent altered ABA- and Ca2þ- regulation of these ion channels is responsible for the ost1 mutant phenotype. Besides protein kinases and protein phosphatases, other diverse enzymes have been implicated in the guard cell responses to ABA, including phospholipases C and D, proteins involved in RN ...
Science Cell Parts Project
Science Cell Parts Project

... parts must be neatly labeled. The entire project must be in color. D. “Cell” phone user guide Create a user’s manual describing how each of the parts found on the “cell phone” work. Be creative! Think about the user’s manual that comes with a cell phone or other electronic. The project must be prese ...
Cell polarity - Philosophical Transactions of the Royal Society B
Cell polarity - Philosophical Transactions of the Royal Society B

... different regions, apical and basal. Moreover, the plasma membranes are different and can transport molecules in different directions. While there is very good evidence for these differences, there is no evidence for polarity between the two regions in the form, for example, of a gradient or molecul ...
Weber et al_rev Legends of supplementary figures and tables
Weber et al_rev Legends of supplementary figures and tables

... levels, expressed relative to EF1, were determined by quantitative real-time PCR for seedlings grown in liquid culture to comparable size (7-9d). Data represent means of three independent experiments. (b) Seedlings of Col-0 as well as homozygous transgenic PME3-Col-OX and PME3-mut-OX lines were gro ...
7-3 Cell Boundaries
7-3 Cell Boundaries

... • Carrier proteins can change shape to move material from one side of the membrane to the other ...
The extracellular matrix at a glance
The extracellular matrix at a glance

... to a specific protein core (with the exception of hyaluronic acid) (Iozzo and Murdoch, 1996; Schaefer and Schaefer, 2010). PGs have been classified according to their core proteins, localization and GAG composition. The three main families are: small leucine-rich proteoglycans (SLRPs), modular prote ...
Low Intensity and Frequency Pulsed
Low Intensity and Frequency Pulsed

... The search for new therapeutic strategies is particularly active in the field of oncology where standard antineoplastic treatments, based on chemotherapeutic drugs and/or radiotherapy, possess potentially detrimental secondary effects and on their own often fall short of providing a complete and res ...
Cell Structure Lesson: Vacuole
Cell Structure Lesson: Vacuole

Drosophila immune cell migration and adhesion during embryonic
Drosophila immune cell migration and adhesion during embryonic

... PDGF/VEGF-related ligands (Pvfs) have been thought to mediate migration along all three embryonic routes in Drosophila but this idea is now contested. The original hypothesis rested on the findings that each path expresses one of the 3 Pvfs [13,16] and that loss of function of the ligands or their p ...
Cell Review Questions
Cell Review Questions

Positive and negative roles for cddO in cell cycle
Positive and negative roles for cddO in cell cycle

... cdclO-C4 was a specific property conferred by MCB elements, we investigated synchronous cultures of cdclO-C4 and cdclO* strains, each carrying pSPAl78.3M. After growth at 24°C and synchronisation by elutriation, samples were taken for cell number and (3-galactosidase estimation (Fig. 3B). The degree ...
HELIX BIOPHARMA CORP. ANNOUNCES CAR
HELIX BIOPHARMA CORP. ANNOUNCES CAR

... for the prevention and treatment of cancer, is pleased to announce that its poster entitled: CART cell harboring a camelid single domain antibody as a targeting agent to kill tumours expressing VEGFR2 has been accepted for presentation at the AACR Annual Meeting 2017 taking place on April 1 - 5, 201 ...
Scaffolding microdomains and beyond: the function of reggie/flotillin
Scaffolding microdomains and beyond: the function of reggie/flotillin

... (EST) database searches yield multiple tags for this Nterminally truncated form lacking the membrane anchor. However, we and others have not found any major splice variants of reggie-1 in western blots or reverse transcription-polymerase chain reactions (RT-PCRs) of various mammalian cell lines or t ...
Animal Cell Electronmicrographs
Animal Cell Electronmicrographs

... The basement membrane (BM) follows the contour of the base of a mid-gut epithelia cell from the insect Estigmene acrea larva. Note the irregular enfolding of the plasma (PM) and the many mitochondria which lie in the resulting narrow ...
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SULF1

Sulfatase 1, also known as SULF1, is an enzyme which in humans is encoded by the SULF1 gene.Heparan sulfate proteoglycans (HSPGs) act as co-receptors for numerous heparin-binding growth factors and cytokines and are involved in cell signaling. Heparan sulfate 6-O-endo-sulfatases, such as SULF1, selectively remove 6-O-sulfate groups from heparan sulfate. This activity modulates the effects of heparan sulfate by altering binding sites for signaling molecules.
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