Module 1 - Bioinformatics.ca
... pronounced effect on gene expression • e.g. Drug treated vs. untreated cell line • e.g. Wild type versus knock out mice ...
... pronounced effect on gene expression • e.g. Drug treated vs. untreated cell line • e.g. Wild type versus knock out mice ...
Protein-RNA interactions: Structural analysis and functional classes
... structures have been solved. However, the publication of the structure of the 50S and 30S ribosome subunits in 2000,1,2 and the advent of the structural genomics projects means that structural information for more than 350 protein–RNA complexes is currently available. This increased volume of data m ...
... structures have been solved. However, the publication of the structure of the 50S and 30S ribosome subunits in 2000,1,2 and the advent of the structural genomics projects means that structural information for more than 350 protein–RNA complexes is currently available. This increased volume of data m ...
Site-Directed Mutagenesis of the Proposed Catalytic Amino Acids
... from the resulting clone was used to replace the corresponding fragment in Sindbis virus cDNA clone TotollO1 (18) (these enzymes cleave unique sites in the Sindbis virus genome at nucleotides 6917 and 9804, respectively). The resulting clones were checked by dideoxy sequencing for the presence of th ...
... from the resulting clone was used to replace the corresponding fragment in Sindbis virus cDNA clone TotollO1 (18) (these enzymes cleave unique sites in the Sindbis virus genome at nucleotides 6917 and 9804, respectively). The resulting clones were checked by dideoxy sequencing for the presence of th ...
Chapter 08 Lecture PowerPoint
... • Phage infection of bacterium subverts host transcription machinery • In process, establishes a time-dependent, or temporal, program of transcription – First early phage genes are transcribed – This is followed by the later genes – Late in the infectious cycle there is no longer transcription of th ...
... • Phage infection of bacterium subverts host transcription machinery • In process, establishes a time-dependent, or temporal, program of transcription – First early phage genes are transcribed – This is followed by the later genes – Late in the infectious cycle there is no longer transcription of th ...
transcript
... and binds to 2 phage operator regions • CI shuts down transcription of all genes except for cI, gene for l repressor itself • When lysogeny is established the phage DNA integrates into the bacterial genome • A bacterium harboring integrated phage DNA is called a lysogen and the integrated DNA is cal ...
... and binds to 2 phage operator regions • CI shuts down transcription of all genes except for cI, gene for l repressor itself • When lysogeny is established the phage DNA integrates into the bacterial genome • A bacterium harboring integrated phage DNA is called a lysogen and the integrated DNA is cal ...
LECT35 trans1
... Q: So, what’s the big deal? A: There are 20 amino acids; the code is degenerate There could be 4 “isoaccepting tRNAs” competing for one Q: I still don’t see a problem ...
... Q: So, what’s the big deal? A: There are 20 amino acids; the code is degenerate There could be 4 “isoaccepting tRNAs” competing for one Q: I still don’t see a problem ...
UCSC Known Genes (by Jim Kent)
... • Weight of 3 on an edge is good enough. • Single exon gene edges take 4 though. • Rank input RNA by whether refSeq, and number of good edges they use. • If any good edges, output a transcript consisting of the edges used by the first RNA. • Output transcript based on next RNA if the good edges it u ...
... • Weight of 3 on an edge is good enough. • Single exon gene edges take 4 though. • Rank input RNA by whether refSeq, and number of good edges they use. • If any good edges, output a transcript consisting of the edges used by the first RNA. • Output transcript based on next RNA if the good edges it u ...
Nonsense-mediated mRNA decay - Case Western Reserve University
... Upf3 is required to trigger mRNA decay, thus providing a physical and functional link between the EJC and NMD [41!!]. An mRNA can also be recognized as aberrant through mRNP features independent of an EJC, as is the case for several mammalian genes [42]. Moreover, in yeast and Drosophila, for exampl ...
... Upf3 is required to trigger mRNA decay, thus providing a physical and functional link between the EJC and NMD [41!!]. An mRNA can also be recognized as aberrant through mRNP features independent of an EJC, as is the case for several mammalian genes [42]. Moreover, in yeast and Drosophila, for exampl ...
cinnabar - UWL faculty websites
... and l(2)cn s4hs° were performed. All four of these chromosomes lack cn function as well as that of one or more flanking lethal complementation groups (Alexandrov, 1984; Alexandrov & Alexandrov, 1991; Lindsley & Zimm, 1992; Wustmann et al., 1989). Radiolabelled DNA prepared from the )tG3 clone was fo ...
... and l(2)cn s4hs° were performed. All four of these chromosomes lack cn function as well as that of one or more flanking lethal complementation groups (Alexandrov, 1984; Alexandrov & Alexandrov, 1991; Lindsley & Zimm, 1992; Wustmann et al., 1989). Radiolabelled DNA prepared from the )tG3 clone was fo ...
TRANSLATION: How to make proteins?
... mRNA t1/2 = few minutes to 2 hours (yeast) to >90 hours (mammals) ...
... mRNA t1/2 = few minutes to 2 hours (yeast) to >90 hours (mammals) ...
Transcription in Eukaryotes
... • TFIIA and its subunit TFIIJ are not absolutely required for transcription initiation in vitro, so are not considered general transcription factors. ...
... • TFIIA and its subunit TFIIJ are not absolutely required for transcription initiation in vitro, so are not considered general transcription factors. ...
(CH11) Transcription In Eukaryotes (Slides)
... • TFIIA and its subunit TFIIJ are not absolutely required for transcription initiation in vitro, so are not considered general transcription factors. ...
... • TFIIA and its subunit TFIIJ are not absolutely required for transcription initiation in vitro, so are not considered general transcription factors. ...
Chapter Four - people.iup.edu
... • Bacteriocins • Proteins produced by bacteria that inhibit or kill closely related species or even different strains of the same ...
... • Bacteriocins • Proteins produced by bacteria that inhibit or kill closely related species or even different strains of the same ...
Bacterial Ribosomes and Protein Synthesis
... specific protein. If DNA in the nucleus transmits ‘information’ to RNA which then specifies the sequence in which amino acids are joined to form proteins, it is reasonable to hypothesize that the information might involve the sequence of the nucleotides in the DNA and RNA and, therefore, that there ...
... specific protein. If DNA in the nucleus transmits ‘information’ to RNA which then specifies the sequence in which amino acids are joined to form proteins, it is reasonable to hypothesize that the information might involve the sequence of the nucleotides in the DNA and RNA and, therefore, that there ...
TRANSLATION: How to make proteins?
... mRNA t1/2 = few minutes to 2 hours (yeast) to >90 hours (mammals) ...
... mRNA t1/2 = few minutes to 2 hours (yeast) to >90 hours (mammals) ...
10 CODON ANTI- CODON CYTOPLASM RIBOSOME tRNA AMINO
... Protein synthesis is the process of making proteins. The DNA contains the codes to make the proteins, but it CANNOT leave the nucleus. As a result, it must deliver the message in a different way. STEP 1 OF PROTEIN SYNTEHSIS-TRANSCRIPTION. The section of the DNA that contains the code for the needed ...
... Protein synthesis is the process of making proteins. The DNA contains the codes to make the proteins, but it CANNOT leave the nucleus. As a result, it must deliver the message in a different way. STEP 1 OF PROTEIN SYNTEHSIS-TRANSCRIPTION. The section of the DNA that contains the code for the needed ...
Bacterial Ribosomes and Protein Synthesis
... specific protein. If DNA in the nucleus transmits ‘information’ to RNA which then specifies the sequence in which amino acids are joined to form proteins, it is reasonable to hypothesize that the information might involve the sequence of the nucleotides in the DNA and RNA and, therefore, that there ...
... specific protein. If DNA in the nucleus transmits ‘information’ to RNA which then specifies the sequence in which amino acids are joined to form proteins, it is reasonable to hypothesize that the information might involve the sequence of the nucleotides in the DNA and RNA and, therefore, that there ...
Biochemists Break the Code
... EF-Tu (GDP) is inactive and cannot function to bind aa tRNAs. In order to recycle EF-Tu, EF-Ts binds to the EF-Tu (GDP) complex to displace the GDP. GTP then, in turn, displaces EF-Ts. Formation of the new peptide bond (Transpeptidation) Peptide bond formation is simple. It is just a kind of nucleop ...
... EF-Tu (GDP) is inactive and cannot function to bind aa tRNAs. In order to recycle EF-Tu, EF-Ts binds to the EF-Tu (GDP) complex to displace the GDP. GTP then, in turn, displaces EF-Ts. Formation of the new peptide bond (Transpeptidation) Peptide bond formation is simple. It is just a kind of nucleop ...
Polyadenylation
Polyadenylation is the addition of a poly(A) tail to a messenger RNA The poly(A) tail consists of multiple adenosine monophosphates; in other words, it is a stretch of RNA that has only adenine bases. In eukaryotes, polyadenylation is part of the process that produces mature messenger RNA (mRNA) for translation. It, therefore, forms part of the larger process of gene expression.The process of polyadenylation begins as the transcription of a gene finishes, or terminates. The 3'-most segment of the newly made pre-mRNA is first cleaved off by a set of proteins; these proteins then synthesize the poly(A) tail at the RNA's 3' end. In some genes, these proteins may add a poly(A) tail at any one of several possible sites. Therefore, polyadenylation can produce more than one transcript from a single gene (alternative polyadenylation), similar to alternative splicing.The poly(A) tail is important for the nuclear export, translation, and stability of mRNA. The tail is shortened over time, and, when it is short enough, the mRNA is enzymatically degraded. However, in a few cell types, mRNAs with short poly(A) tails are stored for later activation by re-polyadenylation in the cytosol. In contrast, when polyadenylation occurs in bacteria, it promotes RNA degradation. This is also sometimes the case for eukaryotic non-coding RNAs.mRNA molecules in both prokaryotes and eukaryotes have polyadenylated 3'-ends, with the prokaryotic poly(A) tails generally shorter and less mRNA molecules polyadenylated.