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NCEA Level 2 Biology (91159) 2015
NCEA Level 2 Biology (91159) 2015

... Genotype is the set of alleles in our DNA, which is responsible for a particular trait an individual possesses. Biologists use yarrow plants that have identical genotypes because if there are any changes to their phenotype, it can be attributed to environmental factors. The phenotype of an organism ...
Section 8: Genetic Mutations, Ribosome Structure
Section 8: Genetic Mutations, Ribosome Structure

... that a polypeptide is the gene product. One might ask the students if and how their answers would change if the gene product were an RNA. A. A single base-pair substitution would keep the reading frame in tact, so the mutation would likely be less harmful than a mutation that completely alters the r ...
Cell Biology # 4
Cell Biology # 4

... • DNA helices separated into replication bubbles with replication forks at each end – Each strand acts as template for complementary strand ...
mMESSAGE mMACHINE® Kit User Guide
mMESSAGE mMACHINE® Kit User Guide

... amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo, because it has a 7-methyl guanosine cap structure at the 5' end. mMESSAGE mMACHINE® Kit reactions include cap analog [m7G(5')ppp(5')G] in an ultra highyield transcription reaction. The cap analog is incorporated only as th ...
Document
Document

... • tRNAs & rRNAs have long half-life in cell (days) • rRNAs, tRNAs have complex secondary & tertiary structures • RNAs fold into complex 3D shapes • RNAs carry out a diverse array of functions • double stranded & double helical stems & • single-stranded regions (loops) • often contain nonstandard mis ...
The Family of MADS – Box Genes Controlling Flower Development
The Family of MADS – Box Genes Controlling Flower Development

... box proteins consisted of the conserved N-terminal MADS-box, the I domain, the central K domain and the C terminal domain. In Arabidopsis, expression of AP1occurs specifically in the tissues and at the developmental stage in which floral fate is assumed. In the flower, expression of AP1 is restricte ...
DNA and Protein Synthesis WebQuest
DNA and Protein Synthesis WebQuest

... Click the right hand arrow to move through the animation. Answer the following questions. 1. Where does protein synthesis begin? 2. What information do chromosomes contain? ...
Computational Biology - Bioinformatik
Computational Biology - Bioinformatik

... depending on the cell type and pattern of gene expression. Approximately 50% of all annotated human miRNA genes are located in fragile sites or areas of the genome that are associated with cancer. → Mutations in miRNAs or their binding sites may lead to diseases. E.g. Abelson et al. found that a mut ...
17 - Rutgers Chemistry
17 - Rutgers Chemistry

... This assay detects and quantifies mRNA species.9 It is also referred to as a ribonuclease protection assay, and is a type of nuclease protection assay (NPA).10 The procedure for running an RNase protection assay is listed below and is also visualized in Figure 12:3 1) Isolate RNA sample(s) to be exa ...
Positive Control and Catabolite Repression
Positive Control and Catabolite Repression

... • Structural genes: encoding proteins • Regulatory genes: encoding products that interact with other sequences and affect the transcription and translation of these sequences • Regulatory elements: DNA sequences that are not transcribed but play a role in regulating other nucleotide sequences ...
Chapter 6: Statistical Gene Prediction
Chapter 6: Statistical Gene Prediction

... 1. Adenine recognition site marks intron. 2. snRNPs bind around adenine recognition site. 3. The spliceosome thus forms and excises introns in the mRNA. ...
Infectious Bursal Disease Virus (IBDV) genesig
Infectious Bursal Disease Virus (IBDV) genesig

... Use the red pipette to transfer 10µl of Internal Extraction Control RNA to your sample after the lysis buffer has been added then follow the rest of the extraction protocol. If using samples that have already been extracted: Use the grey pipette to transfer 5µl of Internal Extraction Control RNA to ...
Nessun titolo diapositiva
Nessun titolo diapositiva

... The CAP (catabolite activator protein), called also CRP, is able to activate the expression of the lac operon. Activation occurs only in the absence of glucose. Cyclic AMP interacts directly with CAP. When the concentration of glucose is low or absent, the concentration of cAMP is high. With no cAMP ...
Screening of Gene Markers for Forensic Identification of Vaginal
Screening of Gene Markers for Forensic Identification of Vaginal

... 5- Genes having expression according to the CGAP (Cancer Genome Anatomy Project). PCR Primer Sequences: For amplification GAPDH was used as house keeping gene and served as an internal control (Positive Control). Specific forward and reverse primers for genes that were expressed in the vaginal secre ...
SAM Teachers Guide - RI
SAM Teachers Guide - RI

... The Proteins and Nucleic Acids activity focuses on the basic structure of protein, DNA  and RNA—the monomers, the distribution of charges and polarity, and how charged  surfaces contribute to their shape and function. Atomic Structure introduces students to  the positive and negative parts of atoms. ...
Gene Section E2F6 (E2F transcription factor 6) Atlas of Genetics and Cytogenetics
Gene Section E2F6 (E2F transcription factor 6) Atlas of Genetics and Cytogenetics

... encodes the longest mRNA isoform (a), which is 2342 bp. Transcript variant 2 contains an additional segment (2413 bp mRNA), compared to variant 1, that causes a frameshift leading to an early stop codon. This transcript may function in a regulatory role with no protein translated. The predicted prot ...
NATIONAL UNIVERSITY OF SINGAPORE DEPARTMENT OF BIOLOGICAL SCIENCES ADVANCED PLACEMENT TEST
NATIONAL UNIVERSITY OF SINGAPORE DEPARTMENT OF BIOLOGICAL SCIENCES ADVANCED PLACEMENT TEST

... 30. Which statement(s) below is/are correct regarding DNA replication? i. In the lagging strand, many short pieces of DNA are made and this requires many RNA primers and DNA polIII. ii. RNA primers are removed by PolI, which then fills in the gaps with DNA. iii. DNA ligase covalently connects the Ok ...
To summarize, at the replication fork, the leading stand is copied
To summarize, at the replication fork, the leading stand is copied

... • After rRNA genes are transcribed to rRNA in the nucleus, the rRNA and proteins form the subunits in the nucleolus. • The subunits exit the nucleus via nuclear pores. • The large and small subunits join to form a functional ribosome only when they attach to an mRNA molecule. • While very similar i ...
Gill: Transcription Regulation I
Gill: Transcription Regulation I

... http://cs273a.stanford.edu [BejeranoFall13/14] ...
Sequencing and analysis of the nucleocapsid (N) and polymerase (L)
Sequencing and analysis of the nucleocapsid (N) and polymerase (L)

... addition, we have determined the 3' end and the N gene of another, more recent, virulent Middle Eastern strain [Kuwait/82/1 (Taylor, 1986); RPV-Kw]. The deduced amino acid sequences of all four N proteins were aligned (Fig. 1). The N proteins from RPV-R and that from RPV-K are 99.2 % identical, in a ...
Chapter 10 Protein Synthesis
Chapter 10 Protein Synthesis

...  Viruses infect organisms by – binding to receptors on a host’s target cell, – injecting viral genetic material into the cell, and – hijacking the cell’s own molecules and organelles to produce new copies of the virus. ...
The DNA sequence of the fragment Hind.30, 378 bases lcng, fran
The DNA sequence of the fragment Hind.30, 378 bases lcng, fran

... ill vitro transcriptianal terminator sites and a sequence of 171 bases which probably codes for the N terminus of the T7 RNA polymerase. The sequence also codes for the RNase III cleavage site before gene 1. This overlaps with the transcriptianal terminators. The RNA transcript of the sequence about ...
X-inactivation
X-inactivation

... X-related gene expression (NOT Xist) detected only in B (129 allele deleted for Xist) due to no inactivation of the Xist deleted X chromosome (129 is B). This leads to skewed monoallelic expression between clones because A (PGK) is always inactivated and no X-related RNA is observed from it. ...
CHAPTER 7 Molecular Genetics: From DNA to Proteins
CHAPTER 7 Molecular Genetics: From DNA to Proteins

... The double helix shape of DNA, together with Chargaff’s rules, led to a better understanding of DNA. DNA, as a nucleic acid, is made from nucleotide monomers, and the DNA double helix consists of two polynucleotide chains. Each nucleotide consists of a sugar (deoxyribose), a phosphate group, and a n ...
Introns and Exons - Mr. Dalton
Introns and Exons - Mr. Dalton

... the amino acids in the proteins they encode. • Many other mutations have no effect on the organism because they are repaired before protein synthesis occurs. ...
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Non-coding RNA



A non-coding RNA (ncRNA) is an RNA molecule that is not translated into a protein. Less-frequently used synonyms are non-protein-coding RNA (npcRNA), non-messenger RNA (nmRNA) and functional RNA (fRNA). The DNA sequence from which a functional non-coding RNA is transcribed is often called an RNA gene.Non-coding RNA genes include highly abundant and functionally important RNAs such as transfer RNAs (tRNAs) and ribosomal RNAs (rRNAs), as well as RNAs such as snoRNAs, microRNAs, siRNAs, snRNAs, exRNAs, and piRNAs and the long ncRNAs that include examples such as Xist and HOTAIR (see here for a more complete list of ncRNAs). The number of ncRNAs encoded within the human genome is unknown; however, recent transcriptomic and bioinformatic studies suggest the existence of thousands of ncRNAs., but see Since many of the newly identified ncRNAs have not been validated for their function, it is possible that many are non-functional. It is also likely that many ncRNAs are non functional (sometimes referred to as Junk RNA), and are the product of spurious transcription.
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