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The Bioethics of Gene Therapy
The Bioethics of Gene Therapy

Transgenic approaches to microbial disease resistance in crop
Transgenic approaches to microbial disease resistance in crop

... to the 3′ region encoding the LRR [4•]. Differences between the Cf-9 and Cf-4 gene products from tomato, controlling resistance to distinct races of leaf mold, are almost exclusively localized to a region within the LRR [5•]. Comparison of Cf-9 homologs from resistant and susceptible plants reveals ...
Some Natural Language Processing (NLP) Tasks
Some Natural Language Processing (NLP) Tasks

... • stochastic context free grammars (SCFGs) represent variable-length sequences with long-range dependencies ...
Gene conversion and purifying selection shape nucleotide variation
Gene conversion and purifying selection shape nucleotide variation

... When a sample from a male showed two nucleotide peaks at one or more sites (“heterozygous” sites) in the L or M opsin gene, the individual should have two or more loci of the gene with different sequences. In this case, we conservatively inferred two loci for this gene. When a female showed heterozy ...
VistaSeq   Hereditary Cancer Panel
VistaSeq Hereditary Cancer Panel

... Mutations in different genes can cause the same type of cancer; conversely, one gene may be associated with multiple hereditary cancers. ...
Drought response in the spikes of barley: gene expression in the
Drought response in the spikes of barley: gene expression in the

... hybridization data. Total RNA from the original samples used for GeneChip hybridization was treated with DNAse I (Promega) and reverse transcribed with oligo(dT) primer using AMV reverse transcriptase (Promega). Then, 150 ng of cDNA was used for real-time PCR analysis using 2× Maxima™ SYBR Green qPC ...
Microenvironment analysis and identification of magnesium binding
Microenvironment analysis and identification of magnesium binding

... Although Mg2+ binding is driven mostly by electrostatics, it has been shown that non-coulombic properties can in¯uence the electrostatic ®eld (44). We believe that Mg2+ binding sites can be differentiated not only by charge alone, but also by the biochemical and structural properties surrounding the ...
Identification of Mga1, a G‐protein α‐subunit gene involved in
Identification of Mga1, a G‐protein α‐subunit gene involved in

... Targeted deletion of Mga1 in M. ruber M7 Agrobacterium tumefaciens-mediated transformation of M. ruber M7 yielded nine putative disruptants, which exhibited a similar set of morphological changes as described in Cryphonectria parasitica Ga-null mutants (Gao & Nuss, 1996), including reduced vegetativ ...
Advanced Gene Mapping in Eukaryotes
Advanced Gene Mapping in Eukaryotes

... separate until just before the second meiotic division, so the spores in the top half of the ascus always have the centromere from one parent (the ● centromere in this case), and the spores in the bottom half of the ascus always have the centromere from the other parent ( here). Since the two types ...
Visualization with the Integrative Genomics Viewer (IGV)
Visualization with the Integrative Genomics Viewer (IGV)

... /!\ The coverage indicates that this position is covered by 352 reads but I see less than 50 reads in the alignment track. What’s going on?    IGV has default parameters that prevent your computer to crash by using too much memory: it loads only a sample of your alignments (which is supposed to r ...
Non contiguous-finished genome sequence and description of
Non contiguous-finished genome sequence and description of

... Phylogenetic tree highlighting the position of Bacillus jeddahensis strain JCET relative to other type strains within the Bacillus genus. GenBank accession numbers are indicated in parentheses. Sequences were aligned using MUSCLE, and phylogenetic inferences obtained using the maximum-likelihood met ...
CHAPTER ONE  INTRODUCTION 1
CHAPTER ONE INTRODUCTION 1

... Expression of a transgene can be regulated by the strength and tissue specificity of a promoter used in the transgene construct (Potenza et al., 2004; Dietz-Pfeilstetter, 2010) and by inclusion of an intron into the gene construct (Rose and Baliakoff, 2000; Cheng et al., 2004). However, an integrate ...
A standard nomenclature for von Willebrand factor gene mutations
A standard nomenclature for von Willebrand factor gene mutations

... Pseudogene sequence A partial unprocessed VWF pseudogene is located on chromosome 22 at 22p12-pter. The sequence for the majority of the pseudogene, including its 5’ boundary, was published by Mancuso et al in 1991 (8). The sequence spans 21-29 kb and is equivalent to exons 23-34 of the VWF gene (in ...


Multiple Choice - Test Bank Team
Multiple Choice - Test Bank Team

... carried out using a forward primer that hybridizes to a region in the active B gene, and one of several reverse primers, each of which hybridizes to a different location in the locus. The amounts of the PCR products were quantified and normalized to represent the relative crosslinking efficiency in ...
Discovery of MLL1 binding units, their localization to CpG Islands
Discovery of MLL1 binding units, their localization to CpG Islands

... Trithorax complexes transmit the memory of active genes to daughter cells through interactions with Trithorax Response Elements (TREs). However, despite their functional importance, nothing is known about sequence features that may act as TREs in mammalian genomic DNA. Results: By analyzing results ...
Information for women about family history of breast cancer and
Information for women about family history of breast cancer and

... Sometimes there is a fault in one copy of a gene which stops that gene working properly. This fault is called a mutation. There are several genes for which inherited faults may be involved in the development of breast or ovarian cancer. These are genes which normally prevent a woman developing breas ...
this PDF file - Trends in Pharmaceutical Sciences
this PDF file - Trends in Pharmaceutical Sciences

... to environmental stresses (34). Duplication events in Lonicera japonica 1 and Lonicera japonica 2 in clades 9 and 10, Populus tomentosa 3 and Populus stomentosa 1 & 4 in clade 13 and 15, also in Trifolium pratense 1 and Trifolium pratense 3 in subclade 17 and 25 (Figure 1) can be identified in the p ...
Examining the architecture of cellular computing through a
Examining the architecture of cellular computing through a

... Let us begin the comparison with a very simplistic overview of a cell as a multi-layered system, using a computer as the reference system. Molecular and cellular biology research methods, which enabled us to break open a cellular system and see into its internal structure and mechanisms of operation ...
(XX) express twice as many genes as males (XY)?
(XX) express twice as many genes as males (XY)?

... either parent. – Genetic recombination can result from independent assortment of genes located on nonhomologous chromosomes. • Offspring with a phenotype matching one of the parental phenotypes are called parental types • Offspring with nonparental phenotypes (new combinations of traits) are called ...
Importing Next Generation Sequencing Data in LOVD 3.0
Importing Next Generation Sequencing Data in LOVD 3.0

... the variants. These annotations include gene names, transcript accession numbers, predicted protein changes, and functional effects of the variants. Finally, the variants that were detected in an (NGS) experiment may have been studied before. For example, chances are another LOVD contains detailed i ...
T-box-mediated control of the anabolic proline biosynthetic genes of
T-box-mediated control of the anabolic proline biosynthetic genes of

... follows. The 1.05 kb MunI–Sau3A DNA fragment derived from pBKB26, carrying the proB regulatory region and part of the proB coding sequence (B. Kempf and E. Bremer, unpublished data), was inserted into the vector pBluescriptSK2 that had been cleaved with EcoRI and BamHI; this construction yielded pla ...
Parent organism - Office of the Gene Technology Regulator
Parent organism - Office of the Gene Technology Regulator

... guidelines issued by the Regulator. Following harvest, plant material remaining at the site will be removed and/or destroyed. Any cottonseed not kept for research purposes or possible future releases (which would require separate applications) would also be destroyed. Details of the gene construct, ...
WHITE PANICLE1, a Val-tRNA Synthetase
WHITE PANICLE1, a Val-tRNA Synthetase

... chloroplasts develop from proplastids. During chloroplast differentiation, the proplastids first establish the plastidic genetic system. At this stage, NEP transcribes genes encoding PEP and ribosomal proteins and other nuclear genes that are necessary for the development of the plastidic genetic sys ...
Differences in DNA methylation patterns between humans and
Differences in DNA methylation patterns between humans and

... the ratio of the intensities of these two oligonucleotides differed significantly between human and chimpanzee in at least one tissue. By contrast, zero to three differences would be expected due to random experimental and biological variation, as is shown by permutating the species labels for each ...
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RNA-Seq



RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.
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