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combindedAronsMyxoNoSim
combindedAronsMyxoNoSim

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Cells Part 1 - Crossroads Academy

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Biology 220 - Microbiology

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Echromi_lab_intro
Echromi_lab_intro

... The procedure (summarized): Scrape up a patch of cells of each strain. Add CaCl2 transformation buffer and keep the cells on ice. Divide the cells of each strain into three vials. One will get the purple DNA plus a gene for ampicillin resistance One will get the green DNA plus a gene for ampicillin ...
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Chemotaxis



Chemotaxis (from chemo- + taxis) is the movement of an organism in response to a chemical stimulus. Somatic cells, bacteria, and other single-cell or multicellular organisms direct their movements according to certain chemicals in their environment. This is important for bacteria to find food (e.g., glucose) by swimming toward the highest concentration of food molecules, or to flee from poisons (e.g., phenol). In multicellular organisms, chemotaxis is critical to early development (e.g., movement of sperm towards the egg during fertilization) and subsequent phases of development (e.g., migration of neurons or lymphocytes) as well as in normal function. In addition, it has been recognized that mechanisms that allow chemotaxis in animals can be subverted during cancer metastasis.Positive chemotaxis occurs if the movement is toward a higher concentration of the chemical in question; negative chemotaxis if the movement is in the opposite direction. Chemically prompted kinesis (randomly directed or nondirectional) can be called chemokinesis.
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