Summary-1
... Docking in the FAD from the PHBH (1k0i) structure into the active site: Isoaloxazine ring of FAD is nolonger Pierced by SMOA. Adenosine Ring is still Pierced. No room for second ...
... Docking in the FAD from the PHBH (1k0i) structure into the active site: Isoaloxazine ring of FAD is nolonger Pierced by SMOA. Adenosine Ring is still Pierced. No room for second ...
Bimm118 Worksheet #3
... 10. What makes Calcium an effective second messenger (in terms of its cellular concentrations)? Cytosolic Ca2+ is very low (100nM) -steep ion gradient allows for rapid diffusion of Ca2+ across membranes -small changes in Ca2+ permeability can have a prominent effect These properties make Ca2+ an id ...
... 10. What makes Calcium an effective second messenger (in terms of its cellular concentrations)? Cytosolic Ca2+ is very low (100nM) -steep ion gradient allows for rapid diffusion of Ca2+ across membranes -small changes in Ca2+ permeability can have a prominent effect These properties make Ca2+ an id ...
E. coli
... Be able to list enzymes produced by microbes than enhance pathogenicity and virulence as well as describe the effects of these enzymes on the host (i.e., hyaluronidase, collangenase, coagulase, kinase). ...
... Be able to list enzymes produced by microbes than enhance pathogenicity and virulence as well as describe the effects of these enzymes on the host (i.e., hyaluronidase, collangenase, coagulase, kinase). ...
Protein structure-function relationship: Recognition
... Where does the hydrolysis producing Fab and Fc occur? NOTE: the different relative position of Fab and Fc here compared to slide 5. This variation is common among antibody molecules. ...
... Where does the hydrolysis producing Fab and Fc occur? NOTE: the different relative position of Fab and Fc here compared to slide 5. This variation is common among antibody molecules. ...
CD spectroscopy
... active site for the protein. By analyzing which concentrations are required for competing out the binding of other ligands, the affinity for a range of unlabeled ligands can be determined. In this case fluorescence anisotropy – also called polarization - is used for detection, which gives a huge eff ...
... active site for the protein. By analyzing which concentrations are required for competing out the binding of other ligands, the affinity for a range of unlabeled ligands can be determined. In this case fluorescence anisotropy – also called polarization - is used for detection, which gives a huge eff ...
Slide 1
... FIGURE 23-7: A. A schematic of the domain structure of the four subgroups of the mammalian protein kinase C (PKC) family members is shown above the Saccharomyces cerevisiae PKC1. These are the classical isoforms (cPKC), novel isoforms (nPKC), atypical isoforms (aPKC) and the PKC-related kinases (kn ...
... FIGURE 23-7: A. A schematic of the domain structure of the four subgroups of the mammalian protein kinase C (PKC) family members is shown above the Saccharomyces cerevisiae PKC1. These are the classical isoforms (cPKC), novel isoforms (nPKC), atypical isoforms (aPKC) and the PKC-related kinases (kn ...
Protein Secondary Structure
... • Abrupt change in direction of polypeptide backbone, at surface of protein • Stabilized by hydrogen bond across “stem” of hairpin • Sharp turn in space --> steric problems with larger amino acid side chains – often involve Gly, Asn, Ser (small hydrophilic residues) or – Pro (has “built-in” elbow/be ...
... • Abrupt change in direction of polypeptide backbone, at surface of protein • Stabilized by hydrogen bond across “stem” of hairpin • Sharp turn in space --> steric problems with larger amino acid side chains – often involve Gly, Asn, Ser (small hydrophilic residues) or – Pro (has “built-in” elbow/be ...
Protein Tertiary and Quaternary Structure
... Describe the general structure (arrangement of hydrophobic vs. polar R groups) of a globular protein that is embedded in a lipid bilayer (membrane). – Specifically, describe how the primary and secondary structures of a bacterial porin relate to the tertiary structure (and function) of a single pori ...
... Describe the general structure (arrangement of hydrophobic vs. polar R groups) of a globular protein that is embedded in a lipid bilayer (membrane). – Specifically, describe how the primary and secondary structures of a bacterial porin relate to the tertiary structure (and function) of a single pori ...
Summary
... Docking in the FAD from the PHBH (1k0i) structure into the active site: Isoaloxazine ring of FAD is nolonger Pierced by SMOA. Adenosine Ring is still Pierced. No room for second ...
... Docking in the FAD from the PHBH (1k0i) structure into the active site: Isoaloxazine ring of FAD is nolonger Pierced by SMOA. Adenosine Ring is still Pierced. No room for second ...
Метод поиска SDP
... Rakhmaninova AB. (2004) SDPpred: a tool for prediction of amino acid residues that determine differences in functional specificity of homologous proteins. Nucl Acids Res 32(Web Server issue): W424-8. ...
... Rakhmaninova AB. (2004) SDPpred: a tool for prediction of amino acid residues that determine differences in functional specificity of homologous proteins. Nucl Acids Res 32(Web Server issue): W424-8. ...
traducción
... reticulum. These proteins are glycosylated and exposed on the cell surface. Other proteins are anchored in the inner leaflet of the plasma membrane following their translation on free cytosolic ribosomes. The Ras protein illustrated is anchored by a prenyl group attached to the side chain of a C-ter ...
... reticulum. These proteins are glycosylated and exposed on the cell surface. Other proteins are anchored in the inner leaflet of the plasma membrane following their translation on free cytosolic ribosomes. The Ras protein illustrated is anchored by a prenyl group attached to the side chain of a C-ter ...
Alignment between domain region and whole enzyme
... polar or no-polar etc. the residues found at active sites are given in fig.6 and fig.7 ...
... polar or no-polar etc. the residues found at active sites are given in fig.6 and fig.7 ...
Three main topics for this Intro lecture
... • Post-translational modifications often occur on similar motifs in different proteins • PROSITE is a database containing a list of known motifs, each associated with a function or a post-translational modification • You can search PROSITE by looking for each motif it contains in your protein (the s ...
... • Post-translational modifications often occur on similar motifs in different proteins • PROSITE is a database containing a list of known motifs, each associated with a function or a post-translational modification • You can search PROSITE by looking for each motif it contains in your protein (the s ...
ppt
... monitoring the behavior of essentially all residues, has the potential to address the contributions of individual residues. ...
... monitoring the behavior of essentially all residues, has the potential to address the contributions of individual residues. ...
Chap. 4. "Proteins: Three-Dimensional Structure and Function
... group is located within an hydrophobic cleft in myoglobin and in each type of hemoglobin chain. Two histidines in the polypeptides interact with the heme iron. When O2 is bound to the heme, both proteins become a bright red color. 2. Oxygen binding properties of myoglobin and hemoglobin. The oxygena ...
... group is located within an hydrophobic cleft in myoglobin and in each type of hemoglobin chain. Two histidines in the polypeptides interact with the heme iron. When O2 is bound to the heme, both proteins become a bright red color. 2. Oxygen binding properties of myoglobin and hemoglobin. The oxygena ...
UMCG
... Other pathogenic trypanosomatids are whole set of 18 Leishmania species. These cause a spectrum of different tropical diseases, called “leishmaniasis”. Many enzymes in Trypanosoma brucei and Leishmania species are very similar in amino acid seqeunce. With thanks to Wes Van Voorhis ...
... Other pathogenic trypanosomatids are whole set of 18 Leishmania species. These cause a spectrum of different tropical diseases, called “leishmaniasis”. Many enzymes in Trypanosoma brucei and Leishmania species are very similar in amino acid seqeunce. With thanks to Wes Van Voorhis ...
Tertiary and Quaternary Structure
... found in outer membranes of many bacteria and in outer mitochondrial membranes channel-forming proteins permitting passage of ions and small molecules across the membrane globular, but their "solvent" is NOT water -- it’s a membrane core of membrane like a very nonpolar solvent structure of each cha ...
... found in outer membranes of many bacteria and in outer mitochondrial membranes channel-forming proteins permitting passage of ions and small molecules across the membrane globular, but their "solvent" is NOT water -- it’s a membrane core of membrane like a very nonpolar solvent structure of each cha ...
Ig, Struction and Function
... immune response. • The immunoglobulins are a group of glycoproteins present in the serum and tissue fluids of all mammals. ...
... immune response. • The immunoglobulins are a group of glycoproteins present in the serum and tissue fluids of all mammals. ...
Midterm 1 - U of L Class Index
... poly(Glu) and poly(Lys)? Why does the transition occur over such a narrow range of pH? (2 marks) At pH > 6, the carboxyl groups of poly(Glu) are fully deprotonated; repulsion among negatively charged carboxylate groups leads to unfolding of the α helix. However, at pH < 4.2 (pKa of Glu), the side ch ...
... poly(Glu) and poly(Lys)? Why does the transition occur over such a narrow range of pH? (2 marks) At pH > 6, the carboxyl groups of poly(Glu) are fully deprotonated; repulsion among negatively charged carboxylate groups leads to unfolding of the α helix. However, at pH < 4.2 (pKa of Glu), the side ch ...
Diapositive 1
... A signal sequence consists of about 20 amino acids at the N-terminal end of the primary sequence of a protein. It allows insertion of the protein in the membrane of an organelle (endoplasmic reticulum, mitochondria...) or translocation of the protein through one or several organelle membranes. When ...
... A signal sequence consists of about 20 amino acids at the N-terminal end of the primary sequence of a protein. It allows insertion of the protein in the membrane of an organelle (endoplasmic reticulum, mitochondria...) or translocation of the protein through one or several organelle membranes. When ...
Common Plants Toxic to Dogs and Cats
... Australia’s state-of-the-art pet emergency trauma centres ...
... Australia’s state-of-the-art pet emergency trauma centres ...
Virtual Screening
... repulsive regions, taking into account steric and hydrogen bonding interactions ...
... repulsive regions, taking into account steric and hydrogen bonding interactions ...
Virtual Screening
... repulsive regions, taking into account steric and hydrogen bonding interactions ...
... repulsive regions, taking into account steric and hydrogen bonding interactions ...
2015-2016 SMART Team Abstract Booklet.
... is associated with impaired judgment and cognitive functions and can ultimately be lethal, killing almost 75,000 people a year. The N-methyl-Daspartate receptor (NMDAR) protein is a major target of alcohol action in the brain. The Audubon High School SMART (Students Modeling A Research Topic) Team h ...
... is associated with impaired judgment and cognitive functions and can ultimately be lethal, killing almost 75,000 people a year. The N-methyl-Daspartate receptor (NMDAR) protein is a major target of alcohol action in the brain. The Audubon High School SMART (Students Modeling A Research Topic) Team h ...
Anthrax toxin
Anthrax toxin is a three-protein exotoxin secreted by virulent strains of the bacterium, Bacillus anthracis—the causative agent of anthrax. The toxin was first discovered by Harry Smith in 1954. Anthrax toxin is composed of a cell-binding protein, known as protective antigen (PA), and two enzyme components, called edema factor (EF) and lethal factor (LF). These three protein components act together to impart their physiological effects. Assembled complexes containing the toxin components are endocytosed. In the endosome, the enzymatic components of the toxin translocate into the cytoplasm of a target cell. Once in the cytosol, the enzymatic components of the toxin disrupts various immune cell functions, namely cellular signaling and cell migration. The toxin may even induce cell lysis, as is observed for macrophage cells. Anthrax toxin allows the bacteria to evade the immune system, proliferate, and ultimately kill the host animal. Research on anthrax toxin also provides insight into the generation of macromolecular assemblies, and on protein translocation, pore formation, endocytosis, and other biochemical processes.