The Two Major Membrane Skeletal Proteins (Articulins) of Euglena
... Bouck, 1988) from mice immunized with membrane affinity-enriched articulins, mAb isotyping (courtesy of Dr. Thorn Rosiere) using a kit (Hyclone Laboratories, Logan, UT) showed that mAb 3G1 was an IgG3. Polyclonal antisera were generated against protein bands excised from preparative SDS polyacrylami ...
... Bouck, 1988) from mice immunized with membrane affinity-enriched articulins, mAb isotyping (courtesy of Dr. Thorn Rosiere) using a kit (Hyclone Laboratories, Logan, UT) showed that mAb 3G1 was an IgG3. Polyclonal antisera were generated against protein bands excised from preparative SDS polyacrylami ...
Excerpt from J.Mol.Biol.
... structure. Side-chains for Leu248 and Leu249 are omitted for clarity. All potential hydrogen bonds to the pterin moiety are shown as dotted lines. The green model of BH4 illustrates its position in the binary Fe(II)·BH4 complex when superimposed on the ternary structure using conserved active-site r ...
... structure. Side-chains for Leu248 and Leu249 are omitted for clarity. All potential hydrogen bonds to the pterin moiety are shown as dotted lines. The green model of BH4 illustrates its position in the binary Fe(II)·BH4 complex when superimposed on the ternary structure using conserved active-site r ...
Crystal Structure of 4-Chlorobenzoate:CoA Ligase/Synthetase in the
... studies of the CBAL reaction (4) could therefore be rationalized not as fully unique active sites for the two half-reactions but, rather, as mediated by the opposing faces of the C-terminal domain. The adenylate-forming enzymes therefore appear to utilize domain alternation, in which an enzyme adopt ...
... studies of the CBAL reaction (4) could therefore be rationalized not as fully unique active sites for the two half-reactions but, rather, as mediated by the opposing faces of the C-terminal domain. The adenylate-forming enzymes therefore appear to utilize domain alternation, in which an enzyme adopt ...
Membrane pore architecture of the CslF6
... is a major component of the (1-3,1-4)-b-glucan synthase of cereals because knockout mutants of this gene have essentially no (1-3,1-4)-bglucan (4–6). The (1-3,1-4)-b-glucan is a major cell wall component of grasses and is important for human nutrition because of the cholesterollowering properties of ...
... is a major component of the (1-3,1-4)-b-glucan synthase of cereals because knockout mutants of this gene have essentially no (1-3,1-4)-bglucan (4–6). The (1-3,1-4)-b-glucan is a major cell wall component of grasses and is important for human nutrition because of the cholesterollowering properties of ...
CHAPTER 3 STRUCTURAL ELEMENTS OF
... 1. helix-loop-helix: it is present in many Ca2+ binding proteins (calmodulin, parvalbumin, troponin C ) or DNA binding proteins 2. β hairpin : two antiparallel β-strands are kept together by a short loop of 2-5 residues. 3. greek key: four β strands (minimum), two short loops and one long loop are n ...
... 1. helix-loop-helix: it is present in many Ca2+ binding proteins (calmodulin, parvalbumin, troponin C ) or DNA binding proteins 2. β hairpin : two antiparallel β-strands are kept together by a short loop of 2-5 residues. 3. greek key: four β strands (minimum), two short loops and one long loop are n ...
Structural analysis of two enzymes catalysing reverse metabolic
... crystal form contains in its asymmetric unit four ssAnPRT protomers as two pairs of dimers. Each ssAnPRT protomer is composed of two domains (Figures 2, 3 and 4). The small a-helical domain comprises six helices (a1±a4 and a8±a9). The large a/b domain is formed by a central b-sheet (strands b1±b7) a ...
... crystal form contains in its asymmetric unit four ssAnPRT protomers as two pairs of dimers. Each ssAnPRT protomer is composed of two domains (Figures 2, 3 and 4). The small a-helical domain comprises six helices (a1±a4 and a8±a9). The large a/b domain is formed by a central b-sheet (strands b1±b7) a ...
bimat.org
... Sequence analysis of the 4,439-bp cDNA of Lustrin A revealed an open reading frame encoding 1,428 amino acids with the translation initiation codon ATG at nucleotide position 26 (Fig. 1B). At position 23 from this initiation codon there exists an adenine nucleotide, and at position 14 there is a gua ...
... Sequence analysis of the 4,439-bp cDNA of Lustrin A revealed an open reading frame encoding 1,428 amino acids with the translation initiation codon ATG at nucleotide position 26 (Fig. 1B). At position 23 from this initiation codon there exists an adenine nucleotide, and at position 14 there is a gua ...
Chapter 5
... Fig. 5-4 Start with the linear form of fructose (see figure 5.3) and draw the formation of the fructose ring in two steps. Number the carbons. Attach carbon 5 via oxygen to carbon 2. Compare the number of carbons in the fructose and ...
... Fig. 5-4 Start with the linear form of fructose (see figure 5.3) and draw the formation of the fructose ring in two steps. Number the carbons. Attach carbon 5 via oxygen to carbon 2. Compare the number of carbons in the fructose and ...
Chapter 1 Macromolecular Structure and Dynamics
... Without altering more than 50% of the sequence, design a protein with a completely different fold ...
... Without altering more than 50% of the sequence, design a protein with a completely different fold ...
Carcinogens:
... Click here for a list of toxins as determined by the State of California and the EPA: pdf ACUTELY TOXIC SUBSTANCES Acutely toxic substances produce adverse effects when exposed individuals receive only small doses of that substance for a short period of time. (e.g. hydrogen fluoride). Substances wit ...
... Click here for a list of toxins as determined by the State of California and the EPA: pdf ACUTELY TOXIC SUBSTANCES Acutely toxic substances produce adverse effects when exposed individuals receive only small doses of that substance for a short period of time. (e.g. hydrogen fluoride). Substances wit ...
Principles of Protein Structure
... • The chemical nature of the carboxyl and amino groups of all amino acids permit hydrogen bond formation (stability) and hence defines secondary structures within the protein. • The R group has an impact on the likelihood of secondary structure formation (proline is an extreme case) • This leads to ...
... • The chemical nature of the carboxyl and amino groups of all amino acids permit hydrogen bond formation (stability) and hence defines secondary structures within the protein. • The R group has an impact on the likelihood of secondary structure formation (proline is an extreme case) • This leads to ...
Gene Section TMPRSS2 (transmembrane protease, serine 2) Atlas of Genetics and Cytogenetics
... an integral transmembrane domain and remain cellsurface-associated, even after proteolytic activation of the protease zymogen. Human TTSPs, which consists of 17 members, were grouped into four subfamilies based on similarity in domain structure and phylogenetic analysis of the serine protease domain ...
... an integral transmembrane domain and remain cellsurface-associated, even after proteolytic activation of the protease zymogen. Human TTSPs, which consists of 17 members, were grouped into four subfamilies based on similarity in domain structure and phylogenetic analysis of the serine protease domain ...
Lehninger Principles of Biochemistry
... helix (Table 4-2). Alanine with its small methyl group in its side chain shows the greatest propensity to form an helix under most conditions. In contrast, amino acids such as threonine and asparagine, with bulky groups attached to the ß carbon of the amino acid show a reduced propensity to occu ...
... helix (Table 4-2). Alanine with its small methyl group in its side chain shows the greatest propensity to form an helix under most conditions. In contrast, amino acids such as threonine and asparagine, with bulky groups attached to the ß carbon of the amino acid show a reduced propensity to occu ...
domaination
... • 56% of proteins were correctly predicted to have more than one domain • 42% of predictions are within 20 residues of a true boundary • 49.9% (44.6%) correct boundary predictions per protein ...
... • 56% of proteins were correctly predicted to have more than one domain • 42% of predictions are within 20 residues of a true boundary • 49.9% (44.6%) correct boundary predictions per protein ...
DEFINITIONS - Microbiology Book
... Immunoglobulins:Structure and Function • Definition: Glycoprotein molecules that are produced by plasma cells in response to an immunogen and which function as antibodies ...
... Immunoglobulins:Structure and Function • Definition: Glycoprotein molecules that are produced by plasma cells in response to an immunogen and which function as antibodies ...
DEFINITIONS - bums.ac.ir
... Immunoglobulins:Structure and Function • Definition: Glycoprotein molecules that are produced by plasma cells in response to an immunogen and which function as antibodies ...
... Immunoglobulins:Structure and Function • Definition: Glycoprotein molecules that are produced by plasma cells in response to an immunogen and which function as antibodies ...
Crystal structure of a membrane-bound l-amino acid
... Yu and Qiao, 2012). Structure-guided protein engineering would be helpful to decrease the product inhibition and improve biocatalytic efficiency, specificity, stability and other properties important for industrial applications (Hossain et al., 2014c; Hou et al., 2015b). In this study, we solved the ...
... Yu and Qiao, 2012). Structure-guided protein engineering would be helpful to decrease the product inhibition and improve biocatalytic efficiency, specificity, stability and other properties important for industrial applications (Hossain et al., 2014c; Hou et al., 2015b). In this study, we solved the ...
Trafficking of phosphatidylinositol by phosphatidylinositol transfer
... use PITPs in signal transduction and membrane delivery. Mammalian PITPs, PITPα and PITPβ, are paralogous genes that are 94% similar in sequence. Their structural design demonstrates that they can sequester PtdIns or PtdCho (phosphatidylcholine) in their hydrophobic cavity. To deliver the lipid cargo ...
... use PITPs in signal transduction and membrane delivery. Mammalian PITPs, PITPα and PITPβ, are paralogous genes that are 94% similar in sequence. Their structural design demonstrates that they can sequester PtdIns or PtdCho (phosphatidylcholine) in their hydrophobic cavity. To deliver the lipid cargo ...
ap® biology 2008 scoring guidelines - AP Central
... Part (a): 6 points were earned. The student provides an extensive description for disulfide bonds (1 point) and states that the bonds “stabilize protein structure,” mentioning their involvement in tertiary structures (1 point). Two more points were earned for a thorough description of van der Waals ...
... Part (a): 6 points were earned. The student provides an extensive description for disulfide bonds (1 point) and states that the bonds “stabilize protein structure,” mentioning their involvement in tertiary structures (1 point). Two more points were earned for a thorough description of van der Waals ...
Supplementary Data
... Conserved hydrophobic amino acids (V, I, L, F, M, Y, W) are colored dark green, and residues that can substitute them (A, C, T) are colored light green. Loop-forming amino acids (P, G, D, N, S) are colored yellow. Conserved aromatic residues (Y, W, F) are colored purple, conserved basic and acidic r ...
... Conserved hydrophobic amino acids (V, I, L, F, M, Y, W) are colored dark green, and residues that can substitute them (A, C, T) are colored light green. Loop-forming amino acids (P, G, D, N, S) are colored yellow. Conserved aromatic residues (Y, W, F) are colored purple, conserved basic and acidic r ...
Key To Problem Set 3R
... Details: When inhibitor is added, and the SRP is not working properly, some of the ribosomes making acid hydrolase molecules will become attached to the ER, but some will remain unattached in the cytoplasm. The acid hydrolase molecules made by the attached ribosomes will enter the ER and reach the l ...
... Details: When inhibitor is added, and the SRP is not working properly, some of the ribosomes making acid hydrolase molecules will become attached to the ER, but some will remain unattached in the cytoplasm. The acid hydrolase molecules made by the attached ribosomes will enter the ER and reach the l ...
STRUCTURE AND FUNCTION
... essential component of all living material. From microscopic bacteria to plants and animals, nitrogen is a required component of all biological material. Nitrogen makes up over 70% of the earth’s atmosphere and is found mostly in the elemental N2 gaseous form. For cells and living organisms to be ab ...
... essential component of all living material. From microscopic bacteria to plants and animals, nitrogen is a required component of all biological material. Nitrogen makes up over 70% of the earth’s atmosphere and is found mostly in the elemental N2 gaseous form. For cells and living organisms to be ab ...
Structural and functional features of the intracellular amino
... conserved histidine residue, within the conserved amino-terminal motif, to tyrosine, highlighting the functional importance of this motif. Suppression is observed both when the histidine substitution resides in cis, on the same protein molecule as the dominant mutation or in trans, on different co-e ...
... conserved histidine residue, within the conserved amino-terminal motif, to tyrosine, highlighting the functional importance of this motif. Suppression is observed both when the histidine substitution resides in cis, on the same protein molecule as the dominant mutation or in trans, on different co-e ...
ER, Golgi and Vesicles :
... Of particular importance for the rest of this chapter, is the sequence targeting proteins to the endoplasmic reticulum, and by extension, any proteins destined for the ER, the Golgi apparatus, the cell membrane, vesicles and vesicularly-derived compartments, and secretion out of the cell. Here, in a ...
... Of particular importance for the rest of this chapter, is the sequence targeting proteins to the endoplasmic reticulum, and by extension, any proteins destined for the ER, the Golgi apparatus, the cell membrane, vesicles and vesicularly-derived compartments, and secretion out of the cell. Here, in a ...
Anthrax toxin
Anthrax toxin is a three-protein exotoxin secreted by virulent strains of the bacterium, Bacillus anthracis—the causative agent of anthrax. The toxin was first discovered by Harry Smith in 1954. Anthrax toxin is composed of a cell-binding protein, known as protective antigen (PA), and two enzyme components, called edema factor (EF) and lethal factor (LF). These three protein components act together to impart their physiological effects. Assembled complexes containing the toxin components are endocytosed. In the endosome, the enzymatic components of the toxin translocate into the cytoplasm of a target cell. Once in the cytosol, the enzymatic components of the toxin disrupts various immune cell functions, namely cellular signaling and cell migration. The toxin may even induce cell lysis, as is observed for macrophage cells. Anthrax toxin allows the bacteria to evade the immune system, proliferate, and ultimately kill the host animal. Research on anthrax toxin also provides insight into the generation of macromolecular assemblies, and on protein translocation, pore formation, endocytosis, and other biochemical processes.