the signaling role of the accessory receptors cd2 and cd6 in t cell
... influenced by accessory receptors-ligand engagements that deliver costimulatory signals while strengthening the interaction between the cells. CD6, a type I cell surface glycoprotein, whose expression is largely restricted to peripheral T lymphocytes and medullary thymocytes, has been known to play ...
... influenced by accessory receptors-ligand engagements that deliver costimulatory signals while strengthening the interaction between the cells. CD6, a type I cell surface glycoprotein, whose expression is largely restricted to peripheral T lymphocytes and medullary thymocytes, has been known to play ...
A B23-interacting sequence as a tool to visualize protein interactions
... it was also proposed that B23 could play a role in nucleocytoplasmic transport since it can shuttle between the nucleus and the cytoplasm (Borer et al., 1989; Fankhauser et al., 1991). B23 is phosphorylated before mitosis and this modification reduces RNA binding by B23 (Okuwaki et al., 2002). This ...
... it was also proposed that B23 could play a role in nucleocytoplasmic transport since it can shuttle between the nucleus and the cytoplasm (Borer et al., 1989; Fankhauser et al., 1991). B23 is phosphorylated before mitosis and this modification reduces RNA binding by B23 (Okuwaki et al., 2002). This ...
The N Terminus of Bacterial Elongation Factor Tu
... Permutations of the last four amino acids in elf18 had little effect on activity but swapping VNV (position 12 to 14) with GTI (position 15 to 17) strongly reduced activity (Figure 5). The N-terminal EF-Tu sequences of many species of enteric bacteria as well as those of Erwinia amylovora and E. chr ...
... Permutations of the last four amino acids in elf18 had little effect on activity but swapping VNV (position 12 to 14) with GTI (position 15 to 17) strongly reduced activity (Figure 5). The N-terminal EF-Tu sequences of many species of enteric bacteria as well as those of Erwinia amylovora and E. chr ...
Etude in vitro de la stéatose hépatique induite par la - HAL
... the core protein from the ER (Figure 1). This cleaved core protein remains associated with the cytoplasmic monolayer of the ER membrane via a hydrophobic domain located between aa 120 and aa 175 [17, 49]. This enables it to move along this monolayer towards the surface of the lipid droplets [48]. Th ...
... the core protein from the ER (Figure 1). This cleaved core protein remains associated with the cytoplasmic monolayer of the ER membrane via a hydrophobic domain located between aa 120 and aa 175 [17, 49]. This enables it to move along this monolayer towards the surface of the lipid droplets [48]. Th ...
Homologous and heterologous reconstitution of Golgi to chloroplast
... To begin to identify biochemical requirements for Golgi to chloroplast transport, an in vitro system was developed to reconstitute the transport process. Advantage was taken of the fact that precursors to the cytoplasmically synthesized light harvesting chlorophyll a/b binding protein (pLHCPII) are ...
... To begin to identify biochemical requirements for Golgi to chloroplast transport, an in vitro system was developed to reconstitute the transport process. Advantage was taken of the fact that precursors to the cytoplasmically synthesized light harvesting chlorophyll a/b binding protein (pLHCPII) are ...
WW Domains Provide a Platform for the
... from Ivan Dikic (Goethe University Medical School, Frankfurt, Germany). The doubly Myc-tagged AIP4 constructs were generated by PCR of our previously published constructs (74) with the amino terminus of the published AIP4 sequence (31) replacing the amino-terminal Itch portion of the fusion used in ...
... from Ivan Dikic (Goethe University Medical School, Frankfurt, Germany). The doubly Myc-tagged AIP4 constructs were generated by PCR of our previously published constructs (74) with the amino terminus of the published AIP4 sequence (31) replacing the amino-terminal Itch portion of the fusion used in ...
Homologous and heterologous reconstitution of Golgi to chloroplast
... To begin to identify biochemical requirements for Golgi to chloroplast transport, an in vitro system was developed to reconstitute the transport process. Advantage was taken of the fact that precursors to the cytoplasmically synthesized light harvesting chlorophyll a/b binding protein (pLHCPII) are ...
... To begin to identify biochemical requirements for Golgi to chloroplast transport, an in vitro system was developed to reconstitute the transport process. Advantage was taken of the fact that precursors to the cytoplasmically synthesized light harvesting chlorophyll a/b binding protein (pLHCPII) are ...
Role of Protein Carbonylation in Skeletal Muscle Mass Loss
... dysfunction and impaired muscle mass may predict morbidity and mortality in patients with chronic diseases, regardless of the underlying condition. High levels of oxidants may alter function and structure of key cellular molecules such as proteins, DNA, and lipids, leading to cellular injury and dea ...
... dysfunction and impaired muscle mass may predict morbidity and mortality in patients with chronic diseases, regardless of the underlying condition. High levels of oxidants may alter function and structure of key cellular molecules such as proteins, DNA, and lipids, leading to cellular injury and dea ...
original version
... bound allosteric effectors ADP and ATP (see sections 3), and studies have shown that acidic phospholipids can inhibit the formation of the replicatively active nucleoprotein complex at the origin of chromosomal replication (oriC) in E. coli [49,50] (see section 7). The molar ratios of membrane phosp ...
... bound allosteric effectors ADP and ATP (see sections 3), and studies have shown that acidic phospholipids can inhibit the formation of the replicatively active nucleoprotein complex at the origin of chromosomal replication (oriC) in E. coli [49,50] (see section 7). The molar ratios of membrane phosp ...
Full-Text PDF
... bound allosteric effectors ADP and ATP (see sections 3), and studies have shown that acidic phospholipids can inhibit the formation of the replicatively active nucleoprotein complex at the origin of chromosomal replication (oriC) in E. coli [49,50] (see section 7). The molar ratios of membrane phosp ...
... bound allosteric effectors ADP and ATP (see sections 3), and studies have shown that acidic phospholipids can inhibit the formation of the replicatively active nucleoprotein complex at the origin of chromosomal replication (oriC) in E. coli [49,50] (see section 7). The molar ratios of membrane phosp ...
Nutrient production by symbiotic bacteria
... feeding rates and assimilation of dietary leucine. Twelve replicate symbiotic and aposymbiotic aphids reared to 7 days on the complete diet were transferred individually to a sachet of complete diet supplemented with [14C]leucine and [3H]inulin on a Perspex ring (diameter 3.5 cm, height 0.5 cm) abov ...
... feeding rates and assimilation of dietary leucine. Twelve replicate symbiotic and aposymbiotic aphids reared to 7 days on the complete diet were transferred individually to a sachet of complete diet supplemented with [14C]leucine and [3H]inulin on a Perspex ring (diameter 3.5 cm, height 0.5 cm) abov ...
Article - Andrej Sali
... (Wool et al., 1995; Dresios et al., 2006). The size difference is due primarily to expansion segments in the large subunit rRNA, as mammalian and yeast ribosomes contain a similar number of proteins. The functions of the expansion segments are not well known, but at least one insert is required for ...
... (Wool et al., 1995; Dresios et al., 2006). The size difference is due primarily to expansion segments in the large subunit rRNA, as mammalian and yeast ribosomes contain a similar number of proteins. The functions of the expansion segments are not well known, but at least one insert is required for ...
MARINE ICOSAHEDRAL MEMBRANE-CONTAINING - E
... dependent on their host’s cellular machineries, which they utilise to accomplish their infection-cycle. To ensure efficient reproduction the virus-specific nucleic acid is encapsidated in a protective shell, which functions as a vehicle to encounter a new host. Upon receiving a proper signal followi ...
... dependent on their host’s cellular machineries, which they utilise to accomplish their infection-cycle. To ensure efficient reproduction the virus-specific nucleic acid is encapsidated in a protective shell, which functions as a vehicle to encounter a new host. Upon receiving a proper signal followi ...
Chapter 6 General discussion
... tTG and the UPR Our data, in combination with recent data from others, strongly support the notion that tTG is associated with the ER in various cell-types throughout the body (Piacentini et al., 2014). The physiological function of tTG expression at the ER is at present poorly characterized, but se ...
... tTG and the UPR Our data, in combination with recent data from others, strongly support the notion that tTG is associated with the ER in various cell-types throughout the body (Piacentini et al., 2014). The physiological function of tTG expression at the ER is at present poorly characterized, but se ...
The 14-3-3 gene par-5 is required for germline development and
... functions, and there is still little knowledge about the consequences of 14-3-3 misfunction at the organism level (Porter et al., 2006). 14-3-3 proteins are necessary for proper cell cycle arrest following DNA damage in yeast, flies and mammals (Hermeking and Benzinger, 2006). This function is media ...
... functions, and there is still little knowledge about the consequences of 14-3-3 misfunction at the organism level (Porter et al., 2006). 14-3-3 proteins are necessary for proper cell cycle arrest following DNA damage in yeast, flies and mammals (Hermeking and Benzinger, 2006). This function is media ...
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... of the cytoplasmic macromolecular complexes [6]. Indeed, the concentration of macromolecules in the bacterial cytoplasm is about 300–400 mg/mL [7] representing 30% of the total volume. In the case of the mitochondrial matrix of eukaryotic cells, it amounts to 60% [8]. This organization and the intra ...
... of the cytoplasmic macromolecular complexes [6]. Indeed, the concentration of macromolecules in the bacterial cytoplasm is about 300–400 mg/mL [7] representing 30% of the total volume. In the case of the mitochondrial matrix of eukaryotic cells, it amounts to 60% [8]. This organization and the intra ...
Pericentriolar material structure and dynamics
... centrosome-free Drosophila embryo extracts, D-PLP forms complexes with SAS-4, Cnn and g-tubulin, all proteins discovered to occupy concentric toroidal domains within the interphase PCM [31]. These data suggest that the organization and size of the interphase PCM is established through the deposition ...
... centrosome-free Drosophila embryo extracts, D-PLP forms complexes with SAS-4, Cnn and g-tubulin, all proteins discovered to occupy concentric toroidal domains within the interphase PCM [31]. These data suggest that the organization and size of the interphase PCM is established through the deposition ...
Designing logical codon reassignment
... allowing the dissection of many complex biological processes. These spectroscopic probes, inserted directly into the protein backbone, may act as reporters of change in chemical environment occurring at the amino acid residue level. Examples of such spectroscopically-active amino acid side chains in ...
... allowing the dissection of many complex biological processes. These spectroscopic probes, inserted directly into the protein backbone, may act as reporters of change in chemical environment occurring at the amino acid residue level. Examples of such spectroscopically-active amino acid side chains in ...
Development and evaluation of a reporter system for
... Table 3.1 Bacterial strains, plasmids and oligonucleotide primers used in this study ...
... Table 3.1 Bacterial strains, plasmids and oligonucleotide primers used in this study ...
CASK (LIN2) interacts with Cx43 in wounded skin and their
... indicate that the CASK mainly interacts with the hypophosphorylated form of Cx43. Functional co-regulation of these proteins was found in MDCK cells migrating into a scratch wound, where expression of either protein individually inhibits migration but their coexpression abrogates this inhibitory eff ...
... indicate that the CASK mainly interacts with the hypophosphorylated form of Cx43. Functional co-regulation of these proteins was found in MDCK cells migrating into a scratch wound, where expression of either protein individually inhibits migration but their coexpression abrogates this inhibitory eff ...
Snake venomics of Bothrops punctatus
... with gel densitometric scanning. Results showed that the predominant proteins in this secretion are metalloproteinases (41.5%; SVMP), followed by C-type lectin/lectin-like proteins (16.7%; CTL), bradykinin-potentiating peptide-like peptides (10.7%; PEP), phospholipases A2 of both the D49 (8.0%) and ...
... with gel densitometric scanning. Results showed that the predominant proteins in this secretion are metalloproteinases (41.5%; SVMP), followed by C-type lectin/lectin-like proteins (16.7%; CTL), bradykinin-potentiating peptide-like peptides (10.7%; PEP), phospholipases A2 of both the D49 (8.0%) and ...
Study of the role of estrogen receptor variant, ER36, in non genomic
... I wish to thank all team members of the new Le Romancer Team Farida Nasri for all your solutions and buffers, as well as your constant good humor and your delicious pastries. Thanks for all the plasmids you prepared for me. I also wish to thank Cecile Languilaire, our lab technician, for all her tec ...
... I wish to thank all team members of the new Le Romancer Team Farida Nasri for all your solutions and buffers, as well as your constant good humor and your delicious pastries. Thanks for all the plasmids you prepared for me. I also wish to thank Cecile Languilaire, our lab technician, for all her tec ...
pdf
... dve-1 knockdown by RNAi directed to the gene’s 30 UTR (Figure S1), but it is unable to rescue the lethality associated with a null mutation (data not shown). Heterozygous animals with a deletion allele in dve1(tm259)X were obtained from the National Bioresource Project for C. elegans (Tokyo). The 98 ...
... dve-1 knockdown by RNAi directed to the gene’s 30 UTR (Figure S1), but it is unable to rescue the lethality associated with a null mutation (data not shown). Heterozygous animals with a deletion allele in dve1(tm259)X were obtained from the National Bioresource Project for C. elegans (Tokyo). The 98 ...
Protein Interaction Technical Handbook
... The natural affinity of binding partners for each other is at the core of in vitro methods widely adopted for both interaction discovery and confirmation. In vitro methods span a broad range of techniques. At one end of the range are those methods that can be performed at the bench with basic labora ...
... The natural affinity of binding partners for each other is at the core of in vitro methods widely adopted for both interaction discovery and confirmation. In vitro methods span a broad range of techniques. At one end of the range are those methods that can be performed at the bench with basic labora ...
Protein phosphorylation
Protein phosphorylation is a post-translational modification of proteins in which an amino acid residue is phosphorylated by a protein kinase by the addition of a covalently bound phosphate group. Phosphorylation alters the structural conformation of a protein, causing it to become activated, deactivated, or modifying its function. The reverse reaction of phosphorylation is called dephosphorylation, and is catalyzed by protein phosphatases. Protein kinases and phosphatases work independently and in a balance to regulate the function of proteins. The amino acids most commonly phosphorylated are serine, threonine, and tyrosine in eukaryotes, and histidine in prokaryotes, which play important and well-characterized roles in signaling pathways and metabolism. However, many other amino acids can also be phosphorylated, including arginine, lysine, and cysteine. Protein phosphorylation was first reported in 1906 by Phoebus Levene at the Rockefeller Institute for Medical Research with the discovery of phosphorylated vitellin. However, it was nearly 50 years until the enzymatic phosphorylation of proteins by protein kinases was discovered.