In vivo measurement of cytosolic and mitochondrial pH using a pH
... weak acids or bases (Krebs et al., 1983; Siegumfeldt et al., 2000), as well as the equilibrium distribution of benzoic acid (Kresnowati et al., 2007). However, none of these techniques are organelle specific and thus they are bound to result in measurement of an average cellular pH. Additionally, mo ...
... weak acids or bases (Krebs et al., 1983; Siegumfeldt et al., 2000), as well as the equilibrium distribution of benzoic acid (Kresnowati et al., 2007). However, none of these techniques are organelle specific and thus they are bound to result in measurement of an average cellular pH. Additionally, mo ...
Zinc Transporter of Arabidopsis thaliana AtMTP1 is Localized
... mutant plantlets grew normally in 0 and 1 µM Zn; however, they showed severe chlorosis of leaves and suppression of leaf development in 200 and 500 µM Zn (Fig. 7B). It should be noted that the medium may contain a trace amount of Zn even in the 0 µM Zn culture medium, since no special measures were ...
... mutant plantlets grew normally in 0 and 1 µM Zn; however, they showed severe chlorosis of leaves and suppression of leaf development in 200 and 500 µM Zn (Fig. 7B). It should be noted that the medium may contain a trace amount of Zn even in the 0 µM Zn culture medium, since no special measures were ...
Evolution and Development
... expressed any of the markers associated with vertebrate neural crest cells. They found that, indeed, the pigment cells of the siphons and body wall could be highlighted using two general vertebrate neural crest markers, staining with the HNK-1 antibody [9] and expression of a Zic gene [10]. Collecti ...
... expressed any of the markers associated with vertebrate neural crest cells. They found that, indeed, the pigment cells of the siphons and body wall could be highlighted using two general vertebrate neural crest markers, staining with the HNK-1 antibody [9] and expression of a Zic gene [10]. Collecti ...
Mechanism of polarized lysosome exocytosis in epithelial cells
... exocytosis (Fig. 2A,B). These data are in agreement with studies in non-polarized cells, where actin depolymerization increased and microtubule disruption had no effect on lysosome exocytosis (Jaiswal et al., 2002; Laulagnier et al., 2011). This is because microtubule-based motors are responsible fo ...
... exocytosis (Fig. 2A,B). These data are in agreement with studies in non-polarized cells, where actin depolymerization increased and microtubule disruption had no effect on lysosome exocytosis (Jaiswal et al., 2002; Laulagnier et al., 2011). This is because microtubule-based motors are responsible fo ...
Towards High-Throughput Flim for Protein
... The lower level modules are tied together by higher level functions, such as: multiple-point time lapse; region scanning and mosaicing; and cell finding and revisiting. We have two approaches to these higher level functions. Firstly, the development of custom hardware components allow system functio ...
... The lower level modules are tied together by higher level functions, such as: multiple-point time lapse; region scanning and mosaicing; and cell finding and revisiting. We have two approaches to these higher level functions. Firstly, the development of custom hardware components allow system functio ...
APPLICATION OF AUTOMATIC CELL TRACKING FOR WOUND
... effectiveness of different culture conditions. For further analysis of the effectiveness of the cell culture condtions, more detailed measurements of the cell behaviors are often required. For example, Abbi et al. [3] analyzed the cell migration path to assess the effects of expression of exogenous ...
... effectiveness of different culture conditions. For further analysis of the effectiveness of the cell culture condtions, more detailed measurements of the cell behaviors are often required. For example, Abbi et al. [3] analyzed the cell migration path to assess the effects of expression of exogenous ...
STK31 maintains the undifferentiated state of colon cancer cells
... immunofluorescent staining. Consistent with the IHC results, STK31 was localized in the cytoplasm (Figure 1C). Of note, expression of STK31 was heterogeneous in these two cancer cell lines. Particularly, in Caco2 colonies, high STK31 expression levels were found at the edge of colonies, which becam ...
... immunofluorescent staining. Consistent with the IHC results, STK31 was localized in the cytoplasm (Figure 1C). Of note, expression of STK31 was heterogeneous in these two cancer cell lines. Particularly, in Caco2 colonies, high STK31 expression levels were found at the edge of colonies, which becam ...
Gastrointestinal MALT Lymphoma
... • Includes centrocyte-like cells, monocytoid cells, scattered larger cells ...
... • Includes centrocyte-like cells, monocytoid cells, scattered larger cells ...
quality control lect..
... ensure proper contents compared to the labeled amount. Select a sample of 10 filled containers, and remove any labeling that might be altered in weight during the removal of the container contents. Thoroughly cleanse and dry the outside of the containers by a suitable means, and weigh individually. ...
... ensure proper contents compared to the labeled amount. Select a sample of 10 filled containers, and remove any labeling that might be altered in weight during the removal of the container contents. Thoroughly cleanse and dry the outside of the containers by a suitable means, and weigh individually. ...
Disruption of the long-range GPIIIa Cys5
... been characterized, and 24 members of this heterodimer family have been identified.2,3 Integrins mediate both adhesion and bidirectional transmembrane signaling. The binding of these integrins to soluble macromolecules is tightly regulated by integrin activation or inside-out signaling, also known a ...
... been characterized, and 24 members of this heterodimer family have been identified.2,3 Integrins mediate both adhesion and bidirectional transmembrane signaling. The binding of these integrins to soluble macromolecules is tightly regulated by integrin activation or inside-out signaling, also known a ...
What the Distribution of Cell Lengths in the Root Meristem Does and
... 1974), information that is useful and even quantitative, but not always extracted correctly. Here, we describe how the pattern of cell length relates to cell proliferation in the root meristem, because the essentially one-dimensional growth and strict spatial zonation make this organ an ideal case s ...
... 1974), information that is useful and even quantitative, but not always extracted correctly. Here, we describe how the pattern of cell length relates to cell proliferation in the root meristem, because the essentially one-dimensional growth and strict spatial zonation make this organ an ideal case s ...
GroupMeeting(BioSketch)-CollinsMod-_2005-07
... We have not been able to replicate the results of the Collins lab. We did not use the same strain, however, and it is possible that there is an endogenous factor that is repressing the expression of the GFP … although only when the toggle-switch vector is present. We are deleting the lacI and lambda ...
... We have not been able to replicate the results of the Collins lab. We did not use the same strain, however, and it is possible that there is an endogenous factor that is repressing the expression of the GFP … although only when the toggle-switch vector is present. We are deleting the lacI and lambda ...
The Benzophenanthridine Alkaloid Fagaronine Induces
... the chlorides by passage through an Amberlite IRA 400 (60 mL) column. After concentration under reduced pressure, a residue (212 mg) was obtained. This gave pure fagaronine (25 mg) as bright yellow needles after three crystallizations from a mixture of ethyl acetate-methanol: m. p. 202 8C followed ...
... the chlorides by passage through an Amberlite IRA 400 (60 mL) column. After concentration under reduced pressure, a residue (212 mg) was obtained. This gave pure fagaronine (25 mg) as bright yellow needles after three crystallizations from a mixture of ethyl acetate-methanol: m. p. 202 8C followed ...
Late Activation of Apoptotic Pathways Plays a Negligible Role in
... MDR3 phenotypes and certain -tubulin mutations (3, 8, 13, 14). In vivo studies confirmed that desoxyepothilone B, one of the most potent epothilones, was curative in human tumor xenografts that were refractory to paclitaxel (15, 16). Clinical Phase I and II studies with epothilones are currently on ...
... MDR3 phenotypes and certain -tubulin mutations (3, 8, 13, 14). In vivo studies confirmed that desoxyepothilone B, one of the most potent epothilones, was curative in human tumor xenografts that were refractory to paclitaxel (15, 16). Clinical Phase I and II studies with epothilones are currently on ...
1 Collaborative Control of Cell Cycle Progression by the
... triggers mitosis and its associated events via the concerted phosphorylation of a battery of downstream targets (Nigg 2001). But on its own, activation of the Ras-ERK pathway is insufficient to deregulate cell proliferation: in otherwise normal cells, constitutive ras activation, via forced express ...
... triggers mitosis and its associated events via the concerted phosphorylation of a battery of downstream targets (Nigg 2001). But on its own, activation of the Ras-ERK pathway is insufficient to deregulate cell proliferation: in otherwise normal cells, constitutive ras activation, via forced express ...
Mitosis Meiosis Study Guide
... As their name suggests, CDKs require the presence of cyclins to become active. Cyclins are a family of proteins that have no enzymatic activity of their own but activate CDKs by binding to them. CDKs must also be in a particular phosphorylation state — with some sites phosphorylated and others depho ...
... As their name suggests, CDKs require the presence of cyclins to become active. Cyclins are a family of proteins that have no enzymatic activity of their own but activate CDKs by binding to them. CDKs must also be in a particular phosphorylation state — with some sites phosphorylated and others depho ...
CD44 Mediated Endocytosis of Hyaluronan by Chondrocytes
... with an siRNA directed against CD44 or control siRNA. Following transfection, the cells were cultured with or without IL-1 for 72 hours. As shown in Fig 2, the protein level of CD44 was inhibited by the CD44 siRNA. Interestingly, accumulation of intracellular ITEGE bands was also coordinately dimini ...
... with an siRNA directed against CD44 or control siRNA. Following transfection, the cells were cultured with or without IL-1 for 72 hours. As shown in Fig 2, the protein level of CD44 was inhibited by the CD44 siRNA. Interestingly, accumulation of intracellular ITEGE bands was also coordinately dimini ...
reading and questions: kingdom monera
... When food is scarce or conditions become unfavorable in other ways, some bacteria form a small internal resting cell called an endospore. An endospore consists of hereditary (nuclear) material, a small amount of cytoplasm and a thick protective outer coat. An endospore can survive long periods in wh ...
... When food is scarce or conditions become unfavorable in other ways, some bacteria form a small internal resting cell called an endospore. An endospore consists of hereditary (nuclear) material, a small amount of cytoplasm and a thick protective outer coat. An endospore can survive long periods in wh ...
Kidney Handling of Acids/Bases, and Renal Tubular Acidosis
... • RTA type 2 can be isolated, or as part of Fanconi’s syndrome (reduced PCT reabsorption of glucose, amino acids, uric acid, and phosphate, as well as bicarb) ...
... • RTA type 2 can be isolated, or as part of Fanconi’s syndrome (reduced PCT reabsorption of glucose, amino acids, uric acid, and phosphate, as well as bicarb) ...
The Polymeric Immunoglobulin Receptor Accumulates in
... PCI2 cells stably transfected with plgR were differentiated in the presence of NGF on matrigel-coated chamber slides as described above. For some experiments, cells stably expressing pIgR were transiently transfected with eDNA encoding the human LDL-R before differentiation. Control experiments were ...
... PCI2 cells stably transfected with plgR were differentiated in the presence of NGF on matrigel-coated chamber slides as described above. For some experiments, cells stably expressing pIgR were transiently transfected with eDNA encoding the human LDL-R before differentiation. Control experiments were ...
Biology of macrophages: Regulation of gene
... immune response. They are involved in the pathogeny of a large number of chronic inflammatory diseases (such as rheumatoid arthritis) and autoimmune diseases. Most immune cells act during the initial stages of microbial aggression on the organism. In contrast, macrophages are also involved in the la ...
... immune response. They are involved in the pathogeny of a large number of chronic inflammatory diseases (such as rheumatoid arthritis) and autoimmune diseases. Most immune cells act during the initial stages of microbial aggression on the organism. In contrast, macrophages are also involved in the la ...
Cell Review Questions
... Match the following statements with one of the following words; fill in the space with the letter of the correct word. (A) Mitochondria (E) Cell wall (B) Nucleus (F) Golgi apparatus (C) Endoplasmic reticulum (G) Nuclear membrane (D) Ribosome (I) Cell membrane 1. The part of a cell that allows the tr ...
... Match the following statements with one of the following words; fill in the space with the letter of the correct word. (A) Mitochondria (E) Cell wall (B) Nucleus (F) Golgi apparatus (C) Endoplasmic reticulum (G) Nuclear membrane (D) Ribosome (I) Cell membrane 1. The part of a cell that allows the tr ...
Malaria parasites form filamentous cell-to-cell connections
... described for PC12 and dendritic cells, or are close-ended, like T-cell-specific nanotubes [6]. Several functions were attributed to nanotubes, depending on the cell type, from which they originate. Assigned functions range from trafficking of vesicles or mitochondria, as described for PC12 cells an ...
... described for PC12 and dendritic cells, or are close-ended, like T-cell-specific nanotubes [6]. Several functions were attributed to nanotubes, depending on the cell type, from which they originate. Assigned functions range from trafficking of vesicles or mitochondria, as described for PC12 cells an ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.