Slides - Workforce Development in Stem Cell Research
... with no changes in appearance or growth rate • Hypothesized that all cells were immortal in that they could grow indefinitely without changing. Therefore the mechanisms of aging at the organism level could not have root mechanisms inside the cell. – Other labs couldn’t reproduce Carrel’s results –ex ...
... with no changes in appearance or growth rate • Hypothesized that all cells were immortal in that they could grow indefinitely without changing. Therefore the mechanisms of aging at the organism level could not have root mechanisms inside the cell. – Other labs couldn’t reproduce Carrel’s results –ex ...
Chapter 10 Notes (Sections 1-4)
... Most prokaryotic cells begin to replicate, or copy, their DNA once they have grown to a certain size. ...
... Most prokaryotic cells begin to replicate, or copy, their DNA once they have grown to a certain size. ...
The organic anion transport inhibitor, probenecid, inhibits the
... on ice for 10-20 min and subsequently placed at 25 °C on a rocking table for 45 min to allow sealing of plasma membranes. Cells were then washed four times by centrifugation (1000 g, 2 min) with buffered medium (± probenecid) at 25 °C prior to epifluorescence microscopy. At this stage, samples (0.75 ...
... on ice for 10-20 min and subsequently placed at 25 °C on a rocking table for 45 min to allow sealing of plasma membranes. Cells were then washed four times by centrifugation (1000 g, 2 min) with buffered medium (± probenecid) at 25 °C prior to epifluorescence microscopy. At this stage, samples (0.75 ...
Fluorescent tagging of an endogenous gene by
... Selection of cut site by CRISPR-Cas9: HDR starts and ends with the creation and repair of a DSB. Therefore, it is important to ensure highly efficient generation of DSBs using the crRNA-guided Cas9 nuclease. For HDR experiments, selection of the crRNA target site is a balance between the efficiency ...
... Selection of cut site by CRISPR-Cas9: HDR starts and ends with the creation and repair of a DSB. Therefore, it is important to ensure highly efficient generation of DSBs using the crRNA-guided Cas9 nuclease. For HDR experiments, selection of the crRNA target site is a balance between the efficiency ...
Distribution, function, and prognostic value of cytotoxic T lymphocytes
... BLOOD, 17 NOVEMBER 2011 䡠 VOLUME 118, NUMBER 20 ...
... BLOOD, 17 NOVEMBER 2011 䡠 VOLUME 118, NUMBER 20 ...
Global impact of Salmonella type III secretion effector SteA on host
... induced after invasion and is essential for survival and replication within macrophages [4,5]. S. enterica injects more than thirty T3SS effectors to their host cells and some of them have been shown to manipulate cellular processes such as actin cytoskeleton organization, tight junction alterations ...
... induced after invasion and is essential for survival and replication within macrophages [4,5]. S. enterica injects more than thirty T3SS effectors to their host cells and some of them have been shown to manipulate cellular processes such as actin cytoskeleton organization, tight junction alterations ...
Small and stable peptidic PEGylated quantum dots to - HAL
... molecule tracking requires the development of simple and versatile methods to target individual proteins in a controlled manner, ideally in living cells. To address this challenge, we have prepared small and stable QDs (QD-ND) using a surface coating based on a peptide sequence containing a tricyste ...
... molecule tracking requires the development of simple and versatile methods to target individual proteins in a controlled manner, ideally in living cells. To address this challenge, we have prepared small and stable QDs (QD-ND) using a surface coating based on a peptide sequence containing a tricyste ...
Rat Natural Killer Cell, T Cell and Macrophage Functions after
... increase of splenic lymphocyte proliferation induced by Con A at doses of 1.25, 2.5 and 5 mg/ml, respectively, compared with cell response of ICV-injected saline control. Similarly, SNC 80 caused significant (P , .001) 93 6 11, 106 6 23 and 69 6 16 percent increase of splenic lymphocyte proliferatio ...
... increase of splenic lymphocyte proliferation induced by Con A at doses of 1.25, 2.5 and 5 mg/ml, respectively, compared with cell response of ICV-injected saline control. Similarly, SNC 80 caused significant (P , .001) 93 6 11, 106 6 23 and 69 6 16 percent increase of splenic lymphocyte proliferatio ...
PDF
... nf and ns blast cells are clearly distinct and show little variance (Zhang and Weisblat, 2005). The tightly regulated asymmetry of the nf and ns mitoses entails first a rotation of the mitotic apparatus and then its rearward shift relative to the cell cortex during anaphase. The rearward shift of the ...
... nf and ns blast cells are clearly distinct and show little variance (Zhang and Weisblat, 2005). The tightly regulated asymmetry of the nf and ns mitoses entails first a rotation of the mitotic apparatus and then its rearward shift relative to the cell cortex during anaphase. The rearward shift of the ...
Plant cell wall Composition
... protection against mechanical stress. It is located outside the cell membrane and provides these cells with structural support and protection. A major function of the cell wall, in multicellular organisms, is to act as a pressure vessel, preventing overexpansion when water enters the cell. In other ...
... protection against mechanical stress. It is located outside the cell membrane and provides these cells with structural support and protection. A major function of the cell wall, in multicellular organisms, is to act as a pressure vessel, preventing overexpansion when water enters the cell. In other ...
Actomyosin-based Self-organization of cell internalization during C. elegans gastrulation Open Access
... embryo to form the gut primordium [9,10]. This is followed by the multipolar internalization of mesoderm, primordial germ cells, and ectodermal cells to form the pharynx, body musculature, and neuronal tissue, respectively, all from the ventral side [9,11]. Cell internalization has been mainly studi ...
... embryo to form the gut primordium [9,10]. This is followed by the multipolar internalization of mesoderm, primordial germ cells, and ectodermal cells to form the pharynx, body musculature, and neuronal tissue, respectively, all from the ventral side [9,11]. Cell internalization has been mainly studi ...
Blood memory B cells are disturbed and predict the
... response or intolerance to anti-TNF agents or had had contraindications to anti-TNF therapy. Patients previously receiving etanercept were required to have discontinued therapy for at least 4 weeks; patients previously receiving infliximab or adalimumab were required to have discontinued therapy for ...
... response or intolerance to anti-TNF agents or had had contraindications to anti-TNF therapy. Patients previously receiving etanercept were required to have discontinued therapy for at least 4 weeks; patients previously receiving infliximab or adalimumab were required to have discontinued therapy for ...
for? of Immune Homeostasis: Molecules to Die FOXO Transcription
... cells was reduced and the thymocytes exhibited enhanced apoptosis. These data, together with the fact that mice ectopically expressing an active PI3K variant show enhanced T cell viability and reduced susceptibility to Fas-mediated apoptosis, demonstrate that PI3K plays a central role in lymphocyte ...
... cells was reduced and the thymocytes exhibited enhanced apoptosis. These data, together with the fact that mice ectopically expressing an active PI3K variant show enhanced T cell viability and reduced susceptibility to Fas-mediated apoptosis, demonstrate that PI3K plays a central role in lymphocyte ...
Unequal division in Saccharomyces cerevisiae and its implications
... S. cerevisiae permits a rather dramatic demonstration of the relationship between growth and division. During nutrient starvation parent cells produce extremely small daughters which result from the unequal distribution of mass between parent and bud, a situation not usually encountered in organisms ...
... S. cerevisiae permits a rather dramatic demonstration of the relationship between growth and division. During nutrient starvation parent cells produce extremely small daughters which result from the unequal distribution of mass between parent and bud, a situation not usually encountered in organisms ...
Lecture 10: Introduction to Bacteria (Structure, Growth
... Biofilm (community of bacteria in GI tract compete with pathogenic bacteria for surface area) Biofilms formed by aggregation of bacteria secreting protein or carbohydrate coats (capsules) which combine to surround a colony of bacteria. Some bacteria can form aggregates by pili interactions lead ...
... Biofilm (community of bacteria in GI tract compete with pathogenic bacteria for surface area) Biofilms formed by aggregation of bacteria secreting protein or carbohydrate coats (capsules) which combine to surround a colony of bacteria. Some bacteria can form aggregates by pili interactions lead ...
Pregnancy & Development
... Hundreds of sperm cells must release their acrosomal enzymes before fertilization can occur Acrosomal enzymes cut through the zona pelucida ...
... Hundreds of sperm cells must release their acrosomal enzymes before fertilization can occur Acrosomal enzymes cut through the zona pelucida ...
The role of Pex3p in early events of peroxisome biogenesis in
... in conjunction with other peroxisomal membrane proteins (PMPs)- can accumulate all components necessary to develop the vesicles into normal peroxisomes and thus, provide indirect evidence that the nuclear envelope can generate the template for peroxisome re-introduction. Our present data link to and ...
... in conjunction with other peroxisomal membrane proteins (PMPs)- can accumulate all components necessary to develop the vesicles into normal peroxisomes and thus, provide indirect evidence that the nuclear envelope can generate the template for peroxisome re-introduction. Our present data link to and ...
Transfer of vesicles from Schwann cells to axons: a novel
... 2005; Krämer-Albers et al., 2007; Taylor et al., 2007). It remains to be investigated whether vesicular transfer of Hsp70 to axons confers neuroprotection to stress stimuli and neurodegenerative disorders. Schwann cells are essential during regenerative processes after nerve injury, not only by secr ...
... 2005; Krämer-Albers et al., 2007; Taylor et al., 2007). It remains to be investigated whether vesicular transfer of Hsp70 to axons confers neuroprotection to stress stimuli and neurodegenerative disorders. Schwann cells are essential during regenerative processes after nerve injury, not only by secr ...
Effects of cardioactive drugs on human induced pluripotent stem cell
... Human induced pluripotent stem cells (hiPSC) have enabled a major step forward in pathophysiologic studies of inherited diseases and may also prove to be valuable in in vitro drug testing. Long QT syndrome (LQTS), characterized by prolonged cardiac repolarization and risk of sudden death, may be inh ...
... Human induced pluripotent stem cells (hiPSC) have enabled a major step forward in pathophysiologic studies of inherited diseases and may also prove to be valuable in in vitro drug testing. Long QT syndrome (LQTS), characterized by prolonged cardiac repolarization and risk of sudden death, may be inh ...
Live-cell mass profiling: an emerging approach in
... onstrated and was used to show a critical growth-rate threshold at the G1-to-S transition in mammalian cells23. With microfluidic resona- phase microscopy (QPM); then we discuss digital holography and tors there is generally a trade-off between the number of cells that can quantitative phase tomogra ...
... onstrated and was used to show a critical growth-rate threshold at the G1-to-S transition in mammalian cells23. With microfluidic resona- phase microscopy (QPM); then we discuss digital holography and tors there is generally a trade-off between the number of cells that can quantitative phase tomogra ...
Anatomy & physiology of cells
... function in living things. Cells vary in their shape size, and arrangements but all cells have similar components, each with a particular function. Some of the 100 trillion of cells make up human body. All human cell are microscopic in size, shape and function. The diameter range from 7.5 micrometer ...
... function in living things. Cells vary in their shape size, and arrangements but all cells have similar components, each with a particular function. Some of the 100 trillion of cells make up human body. All human cell are microscopic in size, shape and function. The diameter range from 7.5 micrometer ...
Distribution of Furamidine Analogues in Tumor Cells: Targeting of
... furamidine was used to dissect the molecular mechanisms that control the distribution of the drugs into the cytoplasm or the nucleus of the cells. The amidine (DB75) and the various N-alkyl derivatives were found to accumulate selectively in the cell nuclei. This is also the case for a guanidine der ...
... furamidine was used to dissect the molecular mechanisms that control the distribution of the drugs into the cytoplasm or the nucleus of the cells. The amidine (DB75) and the various N-alkyl derivatives were found to accumulate selectively in the cell nuclei. This is also the case for a guanidine der ...
RNA-dependent disassembly of nuclear bodies
... molecules within nucleoli are not as mobile as nucleolar proteins (Grob et al., 2014). FRAP analysis revealed that the rate of Men ε/β RNA exchange was significantly lower than that of paraspeckle proteins (Mao et al., 2011b). Maintenance of NBs appears to require a constant influx of new RNA molec ...
... molecules within nucleoli are not as mobile as nucleolar proteins (Grob et al., 2014). FRAP analysis revealed that the rate of Men ε/β RNA exchange was significantly lower than that of paraspeckle proteins (Mao et al., 2011b). Maintenance of NBs appears to require a constant influx of new RNA molec ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.