- ISpatula
... In an aqueous environment, the hydrophobic component of the copolymer is expected to segregate into the core of the micelles, while the hydrophilic component forms the corona or outer shell. The hydrophobic micelle core serves as a microenvironment for incorporation of various therapeutic compounds, ...
... In an aqueous environment, the hydrophobic component of the copolymer is expected to segregate into the core of the micelles, while the hydrophilic component forms the corona or outer shell. The hydrophobic micelle core serves as a microenvironment for incorporation of various therapeutic compounds, ...
Positioning the Flagellum at the Center of a Dividing Cell To
... atmospheric oxygen. Once the cells were fixed on a transmission electron microscope (TEM) grid or a light microscope slide, they were stained for observation of their flagella. Microscopy analyses revealed that the individual flagella of cells of strain SS-5 are always positioned at the south pole o ...
... atmospheric oxygen. Once the cells were fixed on a transmission electron microscope (TEM) grid or a light microscope slide, they were stained for observation of their flagella. Microscopy analyses revealed that the individual flagella of cells of strain SS-5 are always positioned at the south pole o ...
Dynamin 2 mediates fluid-phase micropinocytosis in epithelial cells
... HRP recovered to that of control cells, or more (Damke et al., 1995), suggesting that inhibition of Dyn1 could induce a compensatory endocytic pathway for fluid uptake. Indeed, a Cdc42-dependent, Dyn2-independent pathway used to internalize GPI-linked proteins and thought to be a main mechanism of f ...
... HRP recovered to that of control cells, or more (Damke et al., 1995), suggesting that inhibition of Dyn1 could induce a compensatory endocytic pathway for fluid uptake. Indeed, a Cdc42-dependent, Dyn2-independent pathway used to internalize GPI-linked proteins and thought to be a main mechanism of f ...
The roles of two C. elegans HOX co-factor orthologs
... characterized mutations in two genes: ceh-20, which encodes the C. elegans ortholog of the Hox co-factor Extradenticle (Exd/Pbx); and unc-62, which encodes the C. elegans ortholog of the Hox co-factor Homothorax (Hth/Meis/Prep). Like Hox mutations, mutations in these co-factors influence multiple ce ...
... characterized mutations in two genes: ceh-20, which encodes the C. elegans ortholog of the Hox co-factor Extradenticle (Exd/Pbx); and unc-62, which encodes the C. elegans ortholog of the Hox co-factor Homothorax (Hth/Meis/Prep). Like Hox mutations, mutations in these co-factors influence multiple ce ...
Inflating bacterial cells by increased protein synthesis
... For nutrient-limited growth, a negative linear GR dependence is seen, that is, 1/〈P〉 / 1 – k/k0. With k0 2.2/h, this GR dependence corresponds to the proteome fraction reported for constitutively expressed proteins in nutrient-limited growth (Scott et al, 2010). While the proteome fraction of most ...
... For nutrient-limited growth, a negative linear GR dependence is seen, that is, 1/〈P〉 / 1 – k/k0. With k0 2.2/h, this GR dependence corresponds to the proteome fraction reported for constitutively expressed proteins in nutrient-limited growth (Scott et al, 2010). While the proteome fraction of most ...
AP lab 1 Osmosis
... placed into a beaker of distilled water with IKI solution. IKI wick react with starch to form a color change. Prior to placing the full bag into the distilled water with IKI, the water and the bag were tested for the presence of glucose with clinistix reagent. The colors of the solution in the bag a ...
... placed into a beaker of distilled water with IKI solution. IKI wick react with starch to form a color change. Prior to placing the full bag into the distilled water with IKI, the water and the bag were tested for the presence of glucose with clinistix reagent. The colors of the solution in the bag a ...
Chapter 2 Role of the synthase domain of Ags1p in cell wall α
... targeting this enzyme have been developed. The α-glucan synthase Ags1p was identified in the fission yeast S. pombe using a temperature-sensitive mutant strain, ags1-1ts, the cells of which lyse at the restrictive temperature due to a weakened cell wall unable to withstand internal osmotic pressure (H ...
... targeting this enzyme have been developed. The α-glucan synthase Ags1p was identified in the fission yeast S. pombe using a temperature-sensitive mutant strain, ags1-1ts, the cells of which lyse at the restrictive temperature due to a weakened cell wall unable to withstand internal osmotic pressure (H ...
What are the basic functions of microfilaments? Insights from studies
... required for modification, membrane localization, and function of Cdc42p (57). Thus, CDC42p seems to be a key regulator that controls bud site assembly and microfilament polarization. There may be some interplay between bud site selection, assembly, and actin localization because overexpression of C ...
... required for modification, membrane localization, and function of Cdc42p (57). Thus, CDC42p seems to be a key regulator that controls bud site assembly and microfilament polarization. There may be some interplay between bud site selection, assembly, and actin localization because overexpression of C ...
Chapter 5 Practice
... Refer to the illustration above. a. How can you explain the observed relationship between salt concentration and rate of contractile vacuole contraction? Write your answer in the space below. b. If something happened to a paramecium that caused its contractile vacuole to stop contracting, what would ...
... Refer to the illustration above. a. How can you explain the observed relationship between salt concentration and rate of contractile vacuole contraction? Write your answer in the space below. b. If something happened to a paramecium that caused its contractile vacuole to stop contracting, what would ...
LETTERS
... cells (Fig. 2t). After a 28-hour reincubation period, the Hoxb1overexpressing cells that had been grafted posteriorly were found to extend more anteriorly than the Hoxb9-overexpressing cells (Fig. 2u). In similar experiments in which only control red and green reporters were electroporated, cells gr ...
... cells (Fig. 2t). After a 28-hour reincubation period, the Hoxb1overexpressing cells that had been grafted posteriorly were found to extend more anteriorly than the Hoxb9-overexpressing cells (Fig. 2u). In similar experiments in which only control red and green reporters were electroporated, cells gr ...
Standard PDF - Wiley Online Library
... for self-antigens escape, tolerance is maintained by peripheral tolerance mechanisms in lymph nodes, tonsil, or spleen. Peripheral tolerance can occur through different mechanisms like induction of anergy, induction of apoptosis, or suppression through regulatory T cells. If peripheral tolerance is ...
... for self-antigens escape, tolerance is maintained by peripheral tolerance mechanisms in lymph nodes, tonsil, or spleen. Peripheral tolerance can occur through different mechanisms like induction of anergy, induction of apoptosis, or suppression through regulatory T cells. If peripheral tolerance is ...
video slide - Issaquah Connect
... • Prokaryotic cells do not contain a _______ and have their DNA located in a region called the _________ ...
... • Prokaryotic cells do not contain a _______ and have their DNA located in a region called the _________ ...
Historical Background: In 1838, Schwann and - Moodle
... 1944 First in vitro cultures of tobacco used to study adventitious shoot formation (Skoog) 1945 Cultivation of excised stem tips of Asparagus in vitro (Loo) 1946 First whole Lupinus and Tropaeolum plants from shoot tips (Ball) 1948 Formation of adventitious shoots and roots of tobacco determined by ...
... 1944 First in vitro cultures of tobacco used to study adventitious shoot formation (Skoog) 1945 Cultivation of excised stem tips of Asparagus in vitro (Loo) 1946 First whole Lupinus and Tropaeolum plants from shoot tips (Ball) 1948 Formation of adventitious shoots and roots of tobacco determined by ...
Mouse embryonic stem cells can differentiate via multiple
... paths that differ from those in embryos, much of what actually occurs in these new programs remains mysterious. Does DP bypass normal intermediates by short-circuiting the natural lineage, or does it transition through alternative intermediates (Fig. 1a)? Does it diverge only briefly to bypass spe ...
... paths that differ from those in embryos, much of what actually occurs in these new programs remains mysterious. Does DP bypass normal intermediates by short-circuiting the natural lineage, or does it transition through alternative intermediates (Fig. 1a)? Does it diverge only briefly to bypass spe ...
Surviving apoptosis: life–death signaling in single cells
... through interactions within different protein complexes), or have a survival role only when death is inhibited. Some of these proteins (e.g., caspases) belong to multiple signaling pathways (Box 1), while others, such as the TNF family of death ligands, activate parallel but opposing pathways throug ...
... through interactions within different protein complexes), or have a survival role only when death is inhibited. Some of these proteins (e.g., caspases) belong to multiple signaling pathways (Box 1), while others, such as the TNF family of death ligands, activate parallel but opposing pathways throug ...
Synthesis of fluorescent lipid-polymer probes and study of their
... cirrhosis and hepatocellular carcinoma. New drugs released in 2011 raised hope for patients, however they meet serious problems of tolerance. The entry step of hepatitis C virus (HCV) into its host cells (hepatocytes) still needs to be understood. This comprehension is essen ...
... cirrhosis and hepatocellular carcinoma. New drugs released in 2011 raised hope for patients, however they meet serious problems of tolerance. The entry step of hepatitis C virus (HCV) into its host cells (hepatocytes) still needs to be understood. This comprehension is essen ...
Biofilm-grown Mycoplasma mycoides subsp. mycoides SC
... topped tube (Sterlin) with 2 ml of the same solution to give a density of 106 cfu/ml. Triplicate samples were taken of both adherent and planktonic cells at 10 min to enumerate the number of surviving bacteria. Membranes or a 200 μl aliquot of the planktonic cells were aseptically removed from each ...
... topped tube (Sterlin) with 2 ml of the same solution to give a density of 106 cfu/ml. Triplicate samples were taken of both adherent and planktonic cells at 10 min to enumerate the number of surviving bacteria. Membranes or a 200 μl aliquot of the planktonic cells were aseptically removed from each ...
Interaction with PI3-kinase contributes to the cytotoxic activity ofApoptin S Maddika
... (Rohn et al., 2002). However, it was also shown that a C-terminally truncated apoptin mutant, in which the phosphorylation site was deleted, was still able to translocate to the nucleus and induce apoptosis (Guelen et al., 2004). Thus, the interaction with other molecules or additional modifications ...
... (Rohn et al., 2002). However, it was also shown that a C-terminally truncated apoptin mutant, in which the phosphorylation site was deleted, was still able to translocate to the nucleus and induce apoptosis (Guelen et al., 2004). Thus, the interaction with other molecules or additional modifications ...
Kainate Activation of Horizontal, Bipolar, Amacrine, and Ganglion
... appear to express varied mixtures of ␣-amino-3-hydroxyl5-methylisoxazole-4-propionic acid (AMPA), kainic acid (KA), and N-methyl-D-aspartate (NMDA) receptors, and these mixtures may influence stimulus detection thresholds and visual response dynamic ranges. Many more retinal cell types have been des ...
... appear to express varied mixtures of ␣-amino-3-hydroxyl5-methylisoxazole-4-propionic acid (AMPA), kainic acid (KA), and N-methyl-D-aspartate (NMDA) receptors, and these mixtures may influence stimulus detection thresholds and visual response dynamic ranges. Many more retinal cell types have been des ...
Selective Expression of a Novel Surface Molecule by Human Th2
... normal adults by stimulation with PHA or an extract of Dermatophagoides pteronyssinus (Der; 1% (v/v)) (Torii Pharmaceutical, Tokyo, Japan) in the presence of 50 ng/ml of human rIL-4 (Genzyme) and 10 mg/ml of neutralizing anti-IFN-g mAb (K1) (Genzyme) (Th2 line). PBMC cultures were supplemented with ...
... normal adults by stimulation with PHA or an extract of Dermatophagoides pteronyssinus (Der; 1% (v/v)) (Torii Pharmaceutical, Tokyo, Japan) in the presence of 50 ng/ml of human rIL-4 (Genzyme) and 10 mg/ml of neutralizing anti-IFN-g mAb (K1) (Genzyme) (Th2 line). PBMC cultures were supplemented with ...
Plant stem cells: divergent pathways and common themes in shoots
... Clonal analysis has demonstrated that asymmetric division of QC cells generates daughter cells, which either retain QC cell function or replace an adjacent cell [39]. Immediate derivatives of the QC are themselves stem cells, or initials, each of which gives rise to specific tissue types within the ...
... Clonal analysis has demonstrated that asymmetric division of QC cells generates daughter cells, which either retain QC cell function or replace an adjacent cell [39]. Immediate derivatives of the QC are themselves stem cells, or initials, each of which gives rise to specific tissue types within the ...
Sticking the Landing: Probing the Roles of LORELEI
... citrine yellow fluorescent protein (LRE-cYFP; see figure). Transformation with an LREcYFP construct restored seed set in lre mutants to wild-type levels and rescued transmission of the lre mutation through the female gametophyte. Deletion of putative GPI anchor addition domains affected the localiza ...
... citrine yellow fluorescent protein (LRE-cYFP; see figure). Transformation with an LREcYFP construct restored seed set in lre mutants to wild-type levels and rescued transmission of the lre mutation through the female gametophyte. Deletion of putative GPI anchor addition domains affected the localiza ...
Rabbit (polyclonal) Anti-Mouse BID Cleavage Site (59/60) Specific
... 12. Incubate the blocked blot with primary antibody at a 1:1,000 starting dilution in Tris buffered saline supplemented with 1% Ig-free BSA and 0.1% Tween 20 overnight at 4oC or for two hours at room temperature. 13. Wash the blot with several changes of Tris buffered saline supplemented with 0.1% T ...
... 12. Incubate the blocked blot with primary antibody at a 1:1,000 starting dilution in Tris buffered saline supplemented with 1% Ig-free BSA and 0.1% Tween 20 overnight at 4oC or for two hours at room temperature. 13. Wash the blot with several changes of Tris buffered saline supplemented with 0.1% T ...
Cell encapsulation
Cell microencapsulation technology involves immobilization of the cells within a polymeric semi-permeable membrane that permits the bidirectional diffusion of molecules such as the influx of oxygen, nutrients, growth factors etc. essential for cell metabolism and the outward diffusion of waste products and therapeutic proteins. At the same time, the semi-permeable nature of the membrane prevents immune cells and antibodies from destroying the encapsulated cells regarding them as foreign invaders.The main motive of cell encapsulation technology is to overcome the existing problem of graft rejection in tissue engineering applications and thus reduce the need for long-term use of immunosuppressive drugs after an organ transplant to control side effects.