K-Ras is essential for normal fetal liver erythropoiesis
... c-kitⴙ day-13.5 fetal liver cells in response to EPO and KitL. (A) Frequency of erythroid progenitors in WT and K-Ras⫺/⫺ nonfractionated day-13.5 fetal liver cells. Total nucleated fetal liver cells (40 000 cells/mL) were plated for growth of BFU-Es in methylcellulose medium supplemented with 10 ng/ ...
... c-kitⴙ day-13.5 fetal liver cells in response to EPO and KitL. (A) Frequency of erythroid progenitors in WT and K-Ras⫺/⫺ nonfractionated day-13.5 fetal liver cells. Total nucleated fetal liver cells (40 000 cells/mL) were plated for growth of BFU-Es in methylcellulose medium supplemented with 10 ng/ ...
NextGen DNA Sequencing for Cell Line Characterization and
... Are clones cell populations arising from a single isolated cell after transfection ? - Yes Do clones originating from a single transfected cell have identical transgene sequences ? - No Can we assess “monoclonal-like properties” of populations by analyzing SNV fixation into SNPs ? - Yes ...
... Are clones cell populations arising from a single isolated cell after transfection ? - Yes Do clones originating from a single transfected cell have identical transgene sequences ? - No Can we assess “monoclonal-like properties” of populations by analyzing SNV fixation into SNPs ? - Yes ...
Blimp1 regulates development of the posterior forelimb, caudal
... and DNA binding (Keller and Maniatis, 1992; Gyory et al., 2003; Gyory et al., 2004). The evolutionarily conserved PR/SET domain, closely related to histone methyl transferases, recruits additional epigenetic modifiers that cooperatively function to modify chromatin structure and mediate gene silenci ...
... and DNA binding (Keller and Maniatis, 1992; Gyory et al., 2003; Gyory et al., 2004). The evolutionarily conserved PR/SET domain, closely related to histone methyl transferases, recruits additional epigenetic modifiers that cooperatively function to modify chromatin structure and mediate gene silenci ...
Mitosis and Polyploid Cell Formation
... link the loss of p34cdc2/cyclmnB kinase activity with the generation of polyploidy in yeast. The p34@@2kinase is highly conserved between yeast and higher eukaryotes (23) and in mammalian cells may be required for a number of mitotic functions additional to those in yeast. These include chro mosome ...
... link the loss of p34cdc2/cyclmnB kinase activity with the generation of polyploidy in yeast. The p34@@2kinase is highly conserved between yeast and higher eukaryotes (23) and in mammalian cells may be required for a number of mitotic functions additional to those in yeast. These include chro mosome ...
Blank Jeopardy - Lisle CUSD 202
... microscope is 10x and the objective is 45x, when I multiply the two numbers I get 450x. What did I just calculate? ...
... microscope is 10x and the objective is 45x, when I multiply the two numbers I get 450x. What did I just calculate? ...
Partial genetic suppression of a loss-of
... bodies within lysosomes in both neurons and in non-neural tissues (Miley et al., 1978); however, whether these bodies are causative for the disease or are merely correlative is unclear (Warrier et al., 2013). In many but not all types of NCL, the primary protein component of these bodies is mitochon ...
... bodies within lysosomes in both neurons and in non-neural tissues (Miley et al., 1978); however, whether these bodies are causative for the disease or are merely correlative is unclear (Warrier et al., 2013). In many but not all types of NCL, the primary protein component of these bodies is mitochon ...
De novo fatty acid synthesis controls the fate between regulatory T
... glycolytic pathway is an active, lineage-decisive step that is required for the development of effector T (Teff ) cells of the TH1, TH2 and TH17 lineages4. Hence, in the absence of mTOR, or after treatment with the mTOR-specific inhibitor rapamycin, the development of Teff cells is greatly diminishe ...
... glycolytic pathway is an active, lineage-decisive step that is required for the development of effector T (Teff ) cells of the TH1, TH2 and TH17 lineages4. Hence, in the absence of mTOR, or after treatment with the mTOR-specific inhibitor rapamycin, the development of Teff cells is greatly diminishe ...
Roles of 14-3-3 and calmodulin binding in subcellular localization
... Rem2 [5]. Although the Ras-related core domain is conserved, RGK proteins exhibit unique structural and functional features that differ from other GTPases. These include the lack of lipid modification for membrane anchorage, the presence of N- and C-terminal extensions and an unconventional G3 motif ...
... Rem2 [5]. Although the Ras-related core domain is conserved, RGK proteins exhibit unique structural and functional features that differ from other GTPases. These include the lack of lipid modification for membrane anchorage, the presence of N- and C-terminal extensions and an unconventional G3 motif ...
Germline stem cell niches
... been difficult due to their complex anatomic structures, the stem cell niches in other genetic model systems, including Drosophila and C. elegans, were among the first to be defined. In 2000, the germarial tip adjacent to GSCs was defined as the niche to support GSCs in the Drosophila ovary (Xie and ...
... been difficult due to their complex anatomic structures, the stem cell niches in other genetic model systems, including Drosophila and C. elegans, were among the first to be defined. In 2000, the germarial tip adjacent to GSCs was defined as the niche to support GSCs in the Drosophila ovary (Xie and ...
Induction of Mac-2BP by nerve growth factor is
... activates several signaling pathways, including the phosphoinositide-3-kinase (PI3K) and mitogen-activated protein kinase pathways. This also results in the activation of several transcriptional factors (18, 19) including NF-κB (20). NGF activates NF-κB and AP-1 in sympathetic neurons, and NGF-induc ...
... activates several signaling pathways, including the phosphoinositide-3-kinase (PI3K) and mitogen-activated protein kinase pathways. This also results in the activation of several transcriptional factors (18, 19) including NF-κB (20). NGF activates NF-κB and AP-1 in sympathetic neurons, and NGF-induc ...
The zebrafish as a model to study intestinal inflammation
... might play a role in mucosal immunity. The epithelial folds of the posterior region are short and this part does not contain absorptive enterocytes, but is likely involved in water absorption (Wallace et al., 2005). A recent study by Wang et al. performed micro-array analysis the gut of adult zebrafi ...
... might play a role in mucosal immunity. The epithelial folds of the posterior region are short and this part does not contain absorptive enterocytes, but is likely involved in water absorption (Wallace et al., 2005). A recent study by Wang et al. performed micro-array analysis the gut of adult zebrafi ...
Recent developments in atomic force microscopy for underwater
... polylaminate structures. Davis and Harris did AFM measurements on onion and Arabidopsis thaliana cell walls and observed tightly interwoven microfibrils [3]. Ding and Himmel reported AFM imaging of the maize parenchyma cell wall surface, which showed similar random orientation of the microfibrils re ...
... polylaminate structures. Davis and Harris did AFM measurements on onion and Arabidopsis thaliana cell walls and observed tightly interwoven microfibrils [3]. Ding and Himmel reported AFM imaging of the maize parenchyma cell wall surface, which showed similar random orientation of the microfibrils re ...
Cytokinesis in Scytosiphon zygotes - Journal of Cell Science
... algae, the conclusive factor that determines the cytokinetic plane is still unclear because there is no prominent structure, such as the contractile ring in animals and the preprophase band in land plants, associated with it. In Scytosiphon zygotes, cytokinesis always takes place perpendicular to th ...
... algae, the conclusive factor that determines the cytokinetic plane is still unclear because there is no prominent structure, such as the contractile ring in animals and the preprophase band in land plants, associated with it. In Scytosiphon zygotes, cytokinesis always takes place perpendicular to th ...
SUSPENSOR DEVELOPMENT IN GAGEA LUTEA (L.) KER GAWL
... and Świątek, 2012). There have been many studies on the cytochemistry, ultrastructure and metabolic activity of suspensors in many species of flowering plants (for reviews see Yeung and Meinke, 1993; Raghavan, 2006) but there is still little information on the cytoskeleton during the formation and d ...
... and Świątek, 2012). There have been many studies on the cytochemistry, ultrastructure and metabolic activity of suspensors in many species of flowering plants (for reviews see Yeung and Meinke, 1993; Raghavan, 2006) but there is still little information on the cytoskeleton during the formation and d ...
Haematopoietic and immune defects associated with GATA2 mutation
... Fig 1. Role of GATA2 in haematopoietic differentiation. Simplified map of key interactions of GATA2 with selected major lineage-specifying transcription factors. The factors indicated are necessary for downstream differentiation according to knock-out models or are highly expressed in differentiated ...
... Fig 1. Role of GATA2 in haematopoietic differentiation. Simplified map of key interactions of GATA2 with selected major lineage-specifying transcription factors. The factors indicated are necessary for downstream differentiation according to knock-out models or are highly expressed in differentiated ...
feven tigistu-sahle – metabolism of polyunsaturated fatty
... The application of human bone marrow derived mesenchymal stromal cells (hBMSCs) for regenerative or immunomodulatory therapies, e.g. treatment of the graft-versus-host disease, requires in vitro expansion of the cells. The hBMSCs undergo subtle changes during expansion which may compromise their fun ...
... The application of human bone marrow derived mesenchymal stromal cells (hBMSCs) for regenerative or immunomodulatory therapies, e.g. treatment of the graft-versus-host disease, requires in vitro expansion of the cells. The hBMSCs undergo subtle changes during expansion which may compromise their fun ...
Kidney Handling of Acids/Bases, and Renal Tubular Acidosis
... (and generates HCO3- for the ECF) (Image credit: Dr. McLaughlin May 4th 2012 lecture) ...
... (and generates HCO3- for the ECF) (Image credit: Dr. McLaughlin May 4th 2012 lecture) ...
Different involvement of the mitochondrial, plastidial and
... efficient strategies allowing cells to cope with the unavoidable presence of reactive oxygen species (ROS). This led to the evolution of flexible and redundant antioxidant networks controlling cellular ROS levels in all aerobic organisms. The alterations in the components of the antioxidant networks ...
... efficient strategies allowing cells to cope with the unavoidable presence of reactive oxygen species (ROS). This led to the evolution of flexible and redundant antioxidant networks controlling cellular ROS levels in all aerobic organisms. The alterations in the components of the antioxidant networks ...
Cytochemical Locslization of Mercury in
... With the concentration of HgCl, used in our experiments, there is insufficient density of mercury atoms to give enough electron scattering to produce an image in the electron microscope. The formation of the long-chain amido compound draws together separate mercury atoms and this concentration effec ...
... With the concentration of HgCl, used in our experiments, there is insufficient density of mercury atoms to give enough electron scattering to produce an image in the electron microscope. The formation of the long-chain amido compound draws together separate mercury atoms and this concentration effec ...
An Overview of the Muscle Cell Cytoskeleton
... filaments has not yet been determined. Evidence that some form of lateral transmission of force between adjacent myofibrils and to the cell membrane was provided by Street and Ramsey (1965) and Street (1983). They crushed a muscle fiber, breaking all the myofibrils (Figure 2). The injured fiber was ...
... filaments has not yet been determined. Evidence that some form of lateral transmission of force between adjacent myofibrils and to the cell membrane was provided by Street and Ramsey (1965) and Street (1983). They crushed a muscle fiber, breaking all the myofibrils (Figure 2). The injured fiber was ...
Origin and shaping of the laterality organ in zebrafish - MPI
... extra-embryonic fates. Live multi-photon confocal imaging reveals that surface epithelial cells undergo Nodal/TGFβ signallingdependent ingression at the dorsal germ ring margin prior to gastrulation, to give rise to dorsal forerunner cells (DFCs), the precursors of KV. DFCs then migrate attached to ...
... extra-embryonic fates. Live multi-photon confocal imaging reveals that surface epithelial cells undergo Nodal/TGFβ signallingdependent ingression at the dorsal germ ring margin prior to gastrulation, to give rise to dorsal forerunner cells (DFCs), the precursors of KV. DFCs then migrate attached to ...
File - need help with revision notes?
... within the plant for longer as they are not a close fit for the enzymes that break them down. It is also thought that they may be able to enter cells through transporters in the plasma membrane, but cannot leave through the transporters that would usually allow natural auxins out by facilitated diff ...
... within the plant for longer as they are not a close fit for the enzymes that break them down. It is also thought that they may be able to enter cells through transporters in the plasma membrane, but cannot leave through the transporters that would usually allow natural auxins out by facilitated diff ...
Supplementation of Animal Cell Culture Media
... procedures for handling vary with each form and source (4). Cell-cycle and apoptosis-regulating agents of many types and applications ...
... procedures for handling vary with each form and source (4). Cell-cycle and apoptosis-regulating agents of many types and applications ...
Cellular differentiation
In developmental biology, cellular differentiation isa cell changes from one cell type to another. Most commonly this is a less specialized type becoming a more specialized type, such as during cell growth. Differentiation occurs numerous times during the development of a multicellular organism as it changes from a simple zygote to a complex system of tissues and cell types. Differentiation continues in adulthood as adult stem cells divide and create fully differentiated daughter cells during tissue repair and during normal cell turnover. Some differentiation occurs in response to antigen exposure. Differentiation dramatically changes a cell's size, shape, membrane potential, metabolic activity, and responsiveness to signals. These changes are largely due to highly controlled modifications in gene expression and are the study of epigenetics. With a few exceptions, cellular differentiation almost never involves a change in the DNA sequence itself. Thus, different cells can have very different physical characteristics despite having the same genome.A cell that can differentiate into all cell types of the adult organism is known as pluripotent. Such cells are called embryonic stem cells in animals and meristematic cells in higher plants. A cell that can differentiate into all cell types, including the placental tissue, is known as totipotent. In mammals, only the zygote and subsequent blastomeres are totipotent, while in plants many differentiated cells can become totipotent with simple laboratory techniques. In cytopathology, the level of cellular differentiation is used as a measure of cancer progression. ""Grade"" is a marker of how differentiated a cell in a tumor is.