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Bell Ringer
Bell Ringer

... Genes in cells are made of DNA, which is a complex molecule. The structure of a DNA molecule contains the information that a cell needs to carry out all of its functions. In a way, DNA is like the cell’s encyclopedia. Suppose that you go to the library to do research for a science project. You find ...
Molecular Methods for Evolutionary Genetics
Molecular Methods for Evolutionary Genetics

... polymorphism in a single protein enabled the first molecular evidence of genetic variation. In the 1980s, the advent of DNA sequencing revealed that the amount of genetic variation at a single locus was far greater than expected. Since 2000, the expansion of DNA sequencing to encompass whole genomes ...
Rationale of Genetic Studies Some goals of genetic studies include
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15.2 Regulation of Transcription & Translation

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Lecture 6 S - BEHESHTI MAAL

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... Match the definition on the left with the term on the right: 1. Alternative form of a gene C A. Gamete 2. Body cells such as a skin cell E B. gene 3. Egg or sperm cell A C. allele 4. Process that produces 4 cells G D. Aa 5. A segment of DNA B E. somatic 6. Homozygous alleles F F. AA 7. Heterozygous ...
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Basic Concepts of Human Genetics
Basic Concepts of Human Genetics

... ⎯ The human genome has about 3x109 bps in length. ⎯ 97% of the human genome is non-coding regions called introns. 3% is responsible for controlling the human genetic behavior. The coding region is called extron. ⎯ There are totally about 40,000 genes, over 5000 have been identified. There are much m ...
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... 1. Somatic rearrangement of Ig gene segments occurs in a highly controlled manner 2. Single B-cells become committed to the synthesis of one unique H-chain and one unique L-chain variable domain, which determine their specificities 3. In each of us a huge B-cell repertoire is generated consisting of ...
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... phenotypic forms in a population. Two mechanisms: Heterozygote Advantage – If individuals who are heterozygous at a particular locus have greater survivorship and reproductive success than any type of homozygote, then two or more alleles will be maintained at that locus by natural selection. Frequen ...
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... Since Mendel’s time, our knowledge of the mechanisms of genetic inheritance has grown immensely. For instance, it is now understood than inheriting one allele can, at times, increase the chance of inheriting another or can a affect how and when a trait is expressed in an individual's phenotype. Like ...
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Gene Cloning
Gene Cloning

... • There is a reluctance on the part of some cultures and individuals to accept the concept of transgenesis, without which gene cloning could not be accomplished • Some cloned genes are used in ‘engineering’ food crops, and food safety has become an issue with the public • There has been a move to pa ...
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Site-specific recombinase technology



Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse
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