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Heredity Study Guide
Heredity Study Guide

... 19. What is the difference between genetic engineering and selective breeding? Genetic engineering: the actual DNA is altered in some way by inserting a needed gene directly into a persons cells Selective breeding: specific traits are selected in the parents in order to ensure they are passed to the ...
Life Science vocabulary
Life Science vocabulary

... clone An offspring produced by asexual reproduction that is genetically identifcal, either naturally or through artificial processes. egg cell A cell produced by a female that contains half of the number of chromosomes present in other body cells. A female reproductive cell. fertilization The union ...
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... media post-transformation will prevent growth of non-transformants. • Genes on plasmids that can ...
Biotechnology: Tools and Techniques of the Trade
Biotechnology: Tools and Techniques of the Trade

...  At the end of class today, you will be able to:  Explain what a restriction enzyme is and what role they play in the cell  Explain how restriction enzymes have been used by biologists as a tool in the manipulation of DNA ...
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... You are a researcher trying to determine whether Scenario III or Scenario IV from problem 6 is the more likely mechanism for regulating the gene expression of pGLO. You perform the following experiments. First pGLO alone is digested by DNAase and the fragments are separated by gel electrophoresis. N ...
Unit 7.2 ws
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... stop RNA polymerase from transcribing the genes on the DNA strand. Answer the following questions. For each question, circle the letter of the correct answer. 1. What is the function of the lac operon in E. coli? A. It regulates reproduction. B. It regulates cell division. C. It enables the bacteriu ...
PowerPoint
PowerPoint

... strand (!? - why careful controls are always wise!). 3. Three years later, Mello and Fire (1998) tested whether both the sense and the anti-sense strand together would inhibit or cancel other out. They hit the jackpot: the dsRNA that they inadvertently created inhibited homologous mRNA expression mu ...
BACTERIA TRANSFORMATION LAB (ACTIVITY)
BACTERIA TRANSFORMATION LAB (ACTIVITY)

... One of the ways that bacteria remain genetically diverse is through the naturally occurring processes of transformation. During transformation bacteria take up plasmid DNA from their environment. Plasmids are small, circular pieces DNA that can be exchanged naturally between bacteria. Plasmids may c ...
Biology Homework Chapter 8
Biology Homework Chapter 8

... 1. Why would the knowledge that genes are made of DNA rather than protein be helpful to biochemists trying to isolate and study genes? ...
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... 389-Mb, is an important staple food for more than half of the world’s population and a model plant for other cereal species. We have developed a large-scale Agrobacterium-mediated transformation procedure with a strong positive-negative selection and succeeded in efficient and reproducible targeting ...
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Lecture #9 Date - Biology Junction

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Human Genetic Disorders
Human Genetic Disorders

... • A genetic counselor can prepare a family pedigree or record that shows inheritance patterns over several generations. This can help determine the chance of being a carrier for that disorder. • We also have genetic testing now and we can test for the presence of specific genes known to cause geneti ...
BIOLOGY 210 FALL 2004
BIOLOGY 210 FALL 2004

... Special needs: A student with a verified disability may be entitled to appropriate academic accommodations. Please contact me ASAP and/or the Disabled Student Services office in Craven Hall 5205, ext. 4905, for further assistance. Course goals and requirements: This course is designed for students t ...
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... Gene therapy is expected to play a key role in next-generation medicine by correcting the underlying genetic causes of disease, thereby facilitating personalised medicine. As this technology can address a wide range of medical conditions, along with finding substantive application within plant genet ...
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... resembling mRNA structure but located in DNA Control: amplify DNA, look for ...
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Appendix: Fusion Gene Plasmid Construction

... Transgenic mice containing the targeted IGRP locus could have been generated through microinjection of the modified BAC described above into the pronuclei of one-cell embryos (9). However, this BAC contains several other genes so we chose to generate transgenic animals containing only the region of ...
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Label each of the following as homozygous or heterozygous
Label each of the following as homozygous or heterozygous

...  In dogs, gum coloration is co-dominant, with black coloration, black & pink spotted and pink.  You have a lovely spotted gummed Labrador retriever who has just had 8 pups. Four of the pups have spotted like your dog, and 4 have black gums. 31. What is the likely phenotype of the sneaky neighbor ...
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Genes - Unit3and4Biology
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The Molecular Biology of Gene Function
The Molecular Biology of Gene Function

... to lack of regulatory controls (eg Lr34) OR less pleiotropy due to lack of pathway/function in new species (eg in native species affects 10 pathways but in new species only one is present). In general a gene that has multiple effects also has multiple controls – more closely related more likely to h ...
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... Immunolocalization of Bicoid, Even-skipped and Caudal Proteins ...
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Site-specific recombinase technology



Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse
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