An accessible database for mouse and human whole transcriptome
... Its main advantage is that it has a large dynamic range and requires only basic sample preparation steps (reverse transcription of RNA). Double-stranded DNA dye (such as SYBR) based qPCR quantifies the amount of double-stranded DNA in a sample. A pair of specific primers amplifies a short (usually 6 ...
... Its main advantage is that it has a large dynamic range and requires only basic sample preparation steps (reverse transcription of RNA). Double-stranded DNA dye (such as SYBR) based qPCR quantifies the amount of double-stranded DNA in a sample. A pair of specific primers amplifies a short (usually 6 ...
Full Text
... Alkaline phosphatase (AP) activity, a marker of endoderm differentiation (Fig. 4I) was detected in embryos developed from eggs injected with a high dose of Cs-HNF3 mRNA (Fig. 4J). To examine further whether overexpression of Cs-HNF3 leads to ectopic endoderm differentiation, we took advantage of cle ...
... Alkaline phosphatase (AP) activity, a marker of endoderm differentiation (Fig. 4I) was detected in embryos developed from eggs injected with a high dose of Cs-HNF3 mRNA (Fig. 4J). To examine further whether overexpression of Cs-HNF3 leads to ectopic endoderm differentiation, we took advantage of cle ...
Chromatin DNA Methylayion
... Model 2: The steady state methylation pattern is a dynamic equilibrium between methylase and demethylase activities CH3 ...
... Model 2: The steady state methylation pattern is a dynamic equilibrium between methylase and demethylase activities CH3 ...
Analysis of multiple phenotypes in genome
... to perform standard univariate regression at each biomarker. There is so far no consensus on which method is the best. The primary purpose of our study was to assess the performance of these four approaches. We introduce the four methods in detail and discuss their relative advantages and disadvanta ...
... to perform standard univariate regression at each biomarker. There is so far no consensus on which method is the best. The primary purpose of our study was to assess the performance of these four approaches. We introduce the four methods in detail and discuss their relative advantages and disadvanta ...
Baker, B. S., Hoff, G., Kaufman, T. C., Wolfner, M. W., and Hazelrigg, T. (1991). A cytopgenetic analysis of the doublesex locus and its flanking regions. Genetics 127: 125-138.
... affects female sexual differentiation, but only weakly affects male sexual differentiation. T h e interactions of ~ s x " ~ ~with ' * mutations in other genes affecting sexual differentiation are described. These results are discussed in terms of the recent molecular findings that the dsx locus enco ...
... affects female sexual differentiation, but only weakly affects male sexual differentiation. T h e interactions of ~ s x " ~ ~with ' * mutations in other genes affecting sexual differentiation are described. These results are discussed in terms of the recent molecular findings that the dsx locus enco ...
htp Pseudomonas aeruginosa AMANDA SWANEPOEL
... and Iglewski, 1998; Ramsey and Wozniak, 2005), but it has also been shown to infect plants and insects (Schroth et al., 1992; Rahme et al., 1995). The success of P. aeruginosa in colonizing these diverse environments is attributed to its ability to synthesize a large number of different virulence fa ...
... and Iglewski, 1998; Ramsey and Wozniak, 2005), but it has also been shown to infect plants and insects (Schroth et al., 1992; Rahme et al., 1995). The success of P. aeruginosa in colonizing these diverse environments is attributed to its ability to synthesize a large number of different virulence fa ...
Cys mutation of fibroblast growth factor receptor 3 in mouse
... Figure 5. Effect of bFGF on the growth in culture of metatarsal bones isolated from E17.5 wild-type mice. (A–D) Bones before and after culture for 3 days in the absence (A and B) or presence (C and D) of bFGF (10 ng/ml), respectively. The dark area corresponds to the zone of mineralized hypertrophic ...
... Figure 5. Effect of bFGF on the growth in culture of metatarsal bones isolated from E17.5 wild-type mice. (A–D) Bones before and after culture for 3 days in the absence (A and B) or presence (C and D) of bFGF (10 ng/ml), respectively. The dark area corresponds to the zone of mineralized hypertrophic ...
DpnII - Inv. PCR of miniMos for distribution
... Protocol 2 - Inverse PCR individual inserts There is a very nice and comprehensive protocol that covers how to map Mos1 insertions by Boulin & Bessereau (2007) in Nature Protocols. This protocol is meant as a complement to their protocol because we changed and optimized several parameters which in o ...
... Protocol 2 - Inverse PCR individual inserts There is a very nice and comprehensive protocol that covers how to map Mos1 insertions by Boulin & Bessereau (2007) in Nature Protocols. This protocol is meant as a complement to their protocol because we changed and optimized several parameters which in o ...
Genetic epidemiology of psoriasis and psoriatic arthritis
... recurrence risks among first degree relatives.10 Even in these reports, however, a multifactorial model cannot be ruled out. A non-mendelian mode of transmission referred to as genomic imprinting has also been proposed for psoriasis and PsA,11 12 as it has for several other autoimmune diseases.13 Ge ...
... recurrence risks among first degree relatives.10 Even in these reports, however, a multifactorial model cannot be ruled out. A non-mendelian mode of transmission referred to as genomic imprinting has also been proposed for psoriasis and PsA,11 12 as it has for several other autoimmune diseases.13 Ge ...
A new heavy lanthanide-dependent DNAzyme
... making magnets. It is also used in nuclear reactors due to its ability to strongly absorb neutrons. Erbium is mainly used for making lasers and thulium is used in X-ray devices as a portable source. From these selections, we identified a new class of DNAzymes with many interesting properties. For ex ...
... making magnets. It is also used in nuclear reactors due to its ability to strongly absorb neutrons. Erbium is mainly used for making lasers and thulium is used in X-ray devices as a portable source. From these selections, we identified a new class of DNAzymes with many interesting properties. For ex ...
gsea user guide
... uses the 3 values in class_A and the 15 values in class_B to score the gene by its differential expression. In the signal-tonoise calculation, the mean and variance estimates for the gene are based on different sample sizes; a situation which it would be better to avoid. (If you wish, you can use ex ...
... uses the 3 values in class_A and the 15 values in class_B to score the gene by its differential expression. In the signal-tonoise calculation, the mean and variance estimates for the gene are based on different sample sizes; a situation which it would be better to avoid. (If you wish, you can use ex ...
Section 11.2 Applying Mendel`s Principles
... Mendel’s principles of heredity, observed through patterns of inheritance, form the basis of modern genetics. ...
... Mendel’s principles of heredity, observed through patterns of inheritance, form the basis of modern genetics. ...
The amelogenin gene
... exons 3,5 and 6 by Salido et al.. 1992; Fig. 1) were sequenced and the deduced amino acid sequence was reported. Nakahori et al. (1991a) reported two genomic sequences for human amelogenin from the X- and V-chromosomes. They isolated a Y -specific clone encoding amelogenin which was used as a probe. ...
... exons 3,5 and 6 by Salido et al.. 1992; Fig. 1) were sequenced and the deduced amino acid sequence was reported. Nakahori et al. (1991a) reported two genomic sequences for human amelogenin from the X- and V-chromosomes. They isolated a Y -specific clone encoding amelogenin which was used as a probe. ...
CS790 – Introduction to Bioinformatics
... Prior to cell division, all the genetic instructions must be “copied” so that each new cell will have a complete set DNA polymerase is the enzyme that copies DNA • Reads the old strand in the 3´ to 5´ direction ...
... Prior to cell division, all the genetic instructions must be “copied” so that each new cell will have a complete set DNA polymerase is the enzyme that copies DNA • Reads the old strand in the 3´ to 5´ direction ...
Ectopic overexpression of castor bean LEAFY
... Sequence-based quantitative trait loci (QTL) analysis has associated many rice and maize genes that encode products containing B3 domain genes with traits such as grain yield, seed weight and number, and protein content [29]. However, the proposed associations of B3-domain-containing master regulato ...
... Sequence-based quantitative trait loci (QTL) analysis has associated many rice and maize genes that encode products containing B3 domain genes with traits such as grain yield, seed weight and number, and protein content [29]. However, the proposed associations of B3-domain-containing master regulato ...
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... PHAVOLUTA (PHV) or REVOLUTA (REV). Finally, we show that ATHB2 is part of a complex regulatory circuit directly involving both HD-Zip II and HD-Zip III proteins. Taken together, our study provides evidence that a genetic system consisting of HD-Zip II and HDZip III genes cooperates in establishing b ...
... PHAVOLUTA (PHV) or REVOLUTA (REV). Finally, we show that ATHB2 is part of a complex regulatory circuit directly involving both HD-Zip II and HD-Zip III proteins. Taken together, our study provides evidence that a genetic system consisting of HD-Zip II and HDZip III genes cooperates in establishing b ...
Ubiquitin-Like Protein 5 Positively Regulates Chaperone Gene
... populated with both F1 and developing F2 progeny of the original four animals, allowing us to score the phenotype across three generations of RNAi exposure. The timing of exposure to RNAi was varied to manipulate the level of gene inactivation, which enabled us to overcome lethal or growth-suppressi ...
... populated with both F1 and developing F2 progeny of the original four animals, allowing us to score the phenotype across three generations of RNAi exposure. The timing of exposure to RNAi was varied to manipulate the level of gene inactivation, which enabled us to overcome lethal or growth-suppressi ...
LIST OF CHECK-UP QUESTIONS for
... c) infl uenza CORRECT d) color blindness 7. What type of mutations has the greatest probability to be expressed in the next generation? a) recessive, linked with a sex, which arises in X-chromosome; b) recessive, which arises in autosomes; c) dominant autosomal mutation CORRECT d) all of these avove ...
... c) infl uenza CORRECT d) color blindness 7. What type of mutations has the greatest probability to be expressed in the next generation? a) recessive, linked with a sex, which arises in X-chromosome; b) recessive, which arises in autosomes; c) dominant autosomal mutation CORRECT d) all of these avove ...
Autism and maternally derived aberrations of chromosome 15q
... Of the chronic mental disabilities of childhood, autism is causally least well understood. The former view that autism was rooted in exposure to humorless and perfectionistic parenting has given way to the notion that genetic influences are dominant underlying factors. Still, identification of speci ...
... Of the chronic mental disabilities of childhood, autism is causally least well understood. The former view that autism was rooted in exposure to humorless and perfectionistic parenting has given way to the notion that genetic influences are dominant underlying factors. Still, identification of speci ...
Genetic Control of Cell Chemistry Using Serratia marcescens
... allows us to do this. Wild-type organisms produce an end product utilized by the cell and have incorporated into the genome a control to “turn on” production of the end product by activating the pathway as it is needed and to “turn off” production as soon as sufficient end product accumulates in the ...
... allows us to do this. Wild-type organisms produce an end product utilized by the cell and have incorporated into the genome a control to “turn on” production of the end product by activating the pathway as it is needed and to “turn off” production as soon as sufficient end product accumulates in the ...
Probable presence of an ubiquitous cryptic mitochondrial gene on
... forming cloverleaf secondary structures, which suggests activity in protein synthesis [24]. For example, the usage frequencies of amino acid cognates in regular primate mitochondrial ORFs coevolve with various antisense tRNA properties that are required for proper activity in translation [24]. These ...
... forming cloverleaf secondary structures, which suggests activity in protein synthesis [24]. For example, the usage frequencies of amino acid cognates in regular primate mitochondrial ORFs coevolve with various antisense tRNA properties that are required for proper activity in translation [24]. These ...
Isolation, Characterization and Complementation
... In summary, only two of the mutant classes described by Abou-JaoudC et al. (1978a) were deficient in their ability to reduce nitrite and only the nirD strain was defective in NADHdependent nitrite reductase activity. The phenotype of LCB82 was identical to that of strain JCB203, but although both of ...
... In summary, only two of the mutant classes described by Abou-JaoudC et al. (1978a) were deficient in their ability to reduce nitrite and only the nirD strain was defective in NADHdependent nitrite reductase activity. The phenotype of LCB82 was identical to that of strain JCB203, but although both of ...
Using Genetic Algorithms with Sexual or Asexual Transposition: a
... compared the GA performance when using 1-point, 2-point, uniform crossover or a simple form of transposition. Transposition allowed the GA to reach better results than crossover, even with smaller populations. Later, this preliminary work was enlarged to a test bed containing eighteen test functions ...
... compared the GA performance when using 1-point, 2-point, uniform crossover or a simple form of transposition. Transposition allowed the GA to reach better results than crossover, even with smaller populations. Later, this preliminary work was enlarged to a test bed containing eighteen test functions ...
Multi-petal cyclamen flowers produced by
... ornamental plants would be highly valuable, because this would confer both an interesting appearance and complete sterility, which would prevent the dispersal of transgenic seeds and pollen. Moreover, this trait could be produced in a selected variety with other valuable traits such as perfume or a ...
... ornamental plants would be highly valuable, because this would confer both an interesting appearance and complete sterility, which would prevent the dispersal of transgenic seeds and pollen. Moreover, this trait could be produced in a selected variety with other valuable traits such as perfume or a ...