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Soft X-ray tomography and cryogenic light microscopy - X
Soft X-ray tomography and cryogenic light microscopy - X

... [24,27,28]. Data were collected for these publications using a prototype cryo-rotation stage mounted on XM-1, a general-purpose soft X-ray microscope at the ALS. Since XM-1 was used primarily for magnetism and materials research, it was not optimally configured for biological imaging. The images pro ...
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... Optical Path Difference (OPD): - comparing the OPL for a ray passing in the plane of exit pupil with the chief ray passing through pupil ...
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Ray Optics at a Deep-Subwavelength Scale: A Transformation Optics Approach Seunghoon Han,

... uniquely designed metamaterials and conformal transformation of the electromagnetic (EM) space, molding the flow of light at the deep-subwavelength scale becomes possible. Ray optics is a powerful tool for designing and analyzing optical systems by tracing the ray trajectories in the medium accordin ...
N15_Geom_Optics - University of Arizona
N15_Geom_Optics - University of Arizona

... prisms, blue light bends more than red light. So the same effect must happen in lenses—where one assumes that ray paths are independent of color. The first picture below shows how lenses will have slightly different focal lengths for different colors. This effect is called “chromatic aberration” and ...
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... • two photons absorbed, one emitted • increases resolution by √2 ...
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Seeing an Image

...  Place the paper on the optics table and position the mirror standing up with the silver edge on the line. Make sure everyone in your group can see an image of the arrow.  Where is the object? _______________________________________________________________  Where is the image? ___________________ ...
Deconvolution Microscopy
Deconvolution Microscopy

... in the range of the wavelength of light used. The ultimate goal of cell microscopy is to capture the activity of cell components. In this respect, the resolution of limited numerical aperture wide-field microscopy may not be sufficiently high to optically resolve small organelles. This results in bl ...
EXPERIMENTAL TECHNIQUES
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... Top-Seeded Solution Growth (TSSG) is a flux growth technique commonly used to grow crystals from high temperature solutions. Flux growth methods allow the crystals to grow before the melting temperature is reached and are used when the crystals melt incongruently or when there is a phase transition ...
Off-axis compressed holographic microscopy in low
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... and phase. In biological imaging, images are typically compressible or sparse in some domain due to the homogeneity, compactness and regularity of the structures of interest. Such property can be easily formulated as mathematical constraints on specific image features. CS can be viewed as a data acq ...
Optics 101 for non-optical engineers
Optics 101 for non-optical engineers

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... optimisation method, but with several enhancements [4], such as an extended line search option. The merit function is powerful and flexible. Wizards exist to setup the function, but a whole range of defects can be added by drag and drop. In addition, there is a number of aids [4] such as variable se ...
Spider Silk: The Mother Nature`s Biological Superlens
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OSA journals template (MSWORD)
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... The SFG-OPO was also tested with the PPLN crystal turned so that the pump light first traverses the OPO region of the crystal. The maximum extracted yellow power was 75 mW in this ...
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... LCoS SLMs are proving to be an essential component in a variety of applications such as flexible-grid fiber telecommunication networks, enhanced microscopy and high-resolution adaptive optics (AO). Meadowlark Optics (Meadowlark) has developed a high-resolution, polarization-independent, phase SLM ca ...
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...  Examples of such physical properties include absorption (e.g. light imaging, xrays), reflectivity (e.g. ultrasound, photography), proton density (e.g. MRI), concentration of radionuclides (e.g. nuclear imaging).  In microscopy, intrinsic specimen absorption, emission, and scattering are the quant ...
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... A first, more traditional approach for the realization of such an ultra-stable microwave generator consists in locking one tooth of the optical comb directly on the ultra-stable laser at 1.55 µm: ν N = N ⋅ f rep + f 0 = ν laser ⇒ f rep = (ν laser − f 0 )/ N . This technique necessitates a self-refer ...
Is Cool the Rule for CCDs?
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... Confocal Microscopy. J. B. Pawley. New York, Springer: 918‐931.  Spring, K. R. (2000). "Scientific Imaging with Digital Cameras." BioTechniques 29(1): 70‐76.  Joubert, J., Y. Sabharwal and D. Sharma (2011). "Digital Camera Technologies for Scientific Bio‐ Imaging.Part 3: Noise and Signal‐to‐Noise Ra ...
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... spatially- dependence. The wavelength dependent optical properties of the system components lead to repeat the entire estimation process. This is usually valid for all the methods : ray-tracing, model-based, and P2SF. Figure 2 reports the computational time (CT) required by each P2SF’s stage (SIFs, ...
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... Lasers can produce relatively high power. Indeed some types of laser can produce kilowatts of power. In communication applications, semiconductor lasers of power up to about 20 mW are available Because laser light is produced in parallel beams, a high percentage (50% to 80%) can be transferred into ...
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... 13. Using Huygens’s principle, draw a diagram to show propagation of a wave front originating from a monochromatic point source. 14. Using Huygens’s principle, derive Snell’s law 15. How are Polaroids artificially made? Mention two uses of Polaroids. Draw a graph showing the dependence of intensity ...
生醫奈米影像技術
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... nanometer peak) in quinine and not the entire absorption spectrum. Because the energy associated with fluorescence emission transitions is typically less than that of absorption, the resulting emitted photons have less energy and are shifted to longer wavelengths. This phenomenon is generally known ...
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... The setup for obtaining efficient frequency doubling of the NIR light is sketched in figure 1. The laser diode is described in18. It is mounted p-side up and has two contacts for controlling injection current: One for the ridge waveguide section and one for the tapered amplifier section. A distribut ...
New Optical Filters Improve High-Speed Multicolor
New Optical Filters Improve High-Speed Multicolor

... by the fluorophore and blocks all undesired light outside this band — especially the excitation light. Systems with laser illumination might or might not use an exciter or a dichroic, but most include some variation of these filters. In the absence of any fluorescence, these filters ideally allow no ...
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... Kelvin Force Microscopy (KFM): • Records forces between tip and sample • Measures Surface Potential • Allows indirect measurement of local resistance ...
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Confocal microscopy



Confocal microscopy is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of adding a spatial pinhole placed at the confocal plane of the lens to eliminate out-of-focus light. It enables the reconstruction of three-dimensional structures from the obtained images. This technique has gained popularity in the scientific and industrial communities and typical applications are in life sciences, semiconductor inspection and materials science.
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