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K - Romanian Biotechnological Letters
K - Romanian Biotechnological Letters

... The non-conventional yeast Kluyveromyces lactis has become an excellent alternative yeast model organism [1, 2]. Reconsidered to be a distinct species [3, 4], K. lactis is an ascomyceteous budding yeast that belongs to the endoascomycetales [1]. There are important reasons for the increased attracti ...
The Mean Between Meme and Gene Comparison
The Mean Between Meme and Gene Comparison

... origin of memes paralleling the creation of eukaryotic cells, which paved the way for multicellular life. However, not only are both stories vague and that of the meme’s pure speculation, but memes appear to have evolved from genes themselves rather than being an analogue. In Dennett’s view, memes i ...
SARS: Severe Acute Respiratory Syndrome
SARS: Severe Acute Respiratory Syndrome

... by doing so, are able to become resistant to many of our current vaccines. This is why many different types of SARS viruses were found throughout the world. Viruses are not classified as living species because they lack the proteins to replicate themselves although they do contain a DNA or RNA genom ...
GENtle, a free multi-purpose molecular biology tool
GENtle, a free multi-purpose molecular biology tool

... application of a patch-work of multiple, only partially compatible tools and web services. Commercial software often covers only parts of the required functions, and tends to lock the user into proprietary formats. In my thesis, I have developed GENtle, a free, multi-purpose bioinformatics software, ...
CONNECTIVE TISSUE LABORATORY Center for Medical Genetics
CONNECTIVE TISSUE LABORATORY Center for Medical Genetics

... the COL5A1 gene is the next step. If no COL5A1 null allele is found, a genomic DNA mutation screening of COL5A1 and cDNA mutation screening of COL5A2 are the next option. These mutation screenings are done by fluorescence bidirectional sequencing. For these analyses a new request form is needed. In ...
Gene Section BUB1 (budding uninhibited by benzimidazoles 1 homolog (yeast))
Gene Section BUB1 (budding uninhibited by benzimidazoles 1 homolog (yeast))

Extended Life-Span and Stress Resistance in Drosophila
Extended Life-Span and Stress Resistance in Drosophila

... • Recall that most organisms have two copies of each gene, one inherited from each parent. • Using genetic engineering methods, it is possible to delete or otherwise alter one or both copies of a gene, so that the animal has either one or no working copy of the gene. • A mouse altered in this way is ...
Controlling subcellular delivery to optimize
Controlling subcellular delivery to optimize

... the main goals of our research is to imitate the function of proteins found in nature. Many proteins in signal transduction pathways are localized to one compartment initially, bind to protein partners in the cell (or are signaled by other proteins) and change their location in the cell, leading to ...
Non-invasive prenatal assessment of trisomy 21 by multiplexed
Non-invasive prenatal assessment of trisomy 21 by multiplexed

WebMOTIFS: Web-based integrated motif discovery
WebMOTIFS: Web-based integrated motif discovery

... similar motifs, WebMOTIFS clusters the significant motifs together and computes a single representative motif for each cluster. WebMOTIFS also provides the option of Bayesian motif discovery with THEME. The THEME algorithm searches for motifs consistent with proteins from specified DNA-binding domai ...
5-1 Necleotide Metabolism (purine)
5-1 Necleotide Metabolism (purine)

... (building the bases from simple building blocks) 2. SALVAGE PATHWAYS ...
ppt
ppt

... • 2 reduction reactions after each addition (NADPH) • 16-C Palmitate is typical product • FAS is large enzyme: 2 subunits (one polypeptide each) with 7 catalytic activities and ACP domain • ACP – acyl carrier protein segment (Ser) is joined to a derivative of coenzyme A: • Oriented with phosphopante ...
Table II presents the enzyme activity as well as the... bers of an ordered tetrad.  The strains were grown...
Table II presents the enzyme activity as well as the... bers of an ordered tetrad. The strains were grown...

... and the analog sensitivity of the individual components against 3AT or para-fluorophenylalanine (pFPA) was complemented (test B). The control heterokaryons no 1, 2, 4 and 5 (Table 1) demonstrated that under each condition, the mutant alleles were recessive to their respective wild-type alleles. Grow ...
Article A Distinct Mitochondrial Genome with DUI
Article A Distinct Mitochondrial Genome with DUI

Protein splicing of PRP8 mini-inteins from species of the genus
Protein splicing of PRP8 mini-inteins from species of the genus

Metabolomic and Gene Expression Profiles Exhibit Modular Genetic
Metabolomic and Gene Expression Profiles Exhibit Modular Genetic

... new dataset used confirm expression patterns, 45 genetic lines raised on the control and high-fat dietary treatments were used for the follow-up experiment to confirm the lack of phenotypeexpression correlation across diets. These lines were selected to represent the diversity of dietary reaction norm ...
File
File

... the ability to isolate a specific segment of DNA, modify it in a test tube, and transfer it back into the same or a different cell to create a genetically modified organism represents perhaps the most significant advance in the history of biology, with profound consequences for the future of our, an ...
Isolation of a New Member of the $100 Protein Family: Amino Acid
Isolation of a New Member of the $100 Protein Family: Amino Acid

... Lysates were clarified by centrifugation at 100,000 g, 30 min, divided into four equal aliquots, and incubated with 5 p.g monoclonal anti-calpactin light chain (a), 5 /zg rabbit anti-calpactin I heavy chain (b), 5 t~g rabbit anti-S100L (c), or without antibody (d). After 3 h 15 ~1 of goat anti-mouse ...
Let`s Review!
Let`s Review!

... A woman has her _________ number of eggs (about 7 million) while still a _______ in her mother’s womb. At ______ that number _______ to about __ or ___ million. By ________ that number has _________ to about ________. And of that number _____ ______-_____ ever mature to be __________ by the ovary. ...
Chromosomes, Chromosome Anomalies
Chromosomes, Chromosome Anomalies

... either as they were: restitution or, in case of 2 (or more) breaks, with interactive re-joining to make a structural aberration (exchanges). Initial breaks are thought to be at the level of the DNA, and are probably frequent events. DNA repair then occurs. For various reasons, DNA repair is insuffic ...
First Title - Buckeye Valley
First Title - Buckeye Valley

... expression of the genetic information. ...
Ch. 33 Synthesis of Fatty acids, Triacylglycerols, Membrane lipids
Ch. 33 Synthesis of Fatty acids, Triacylglycerols, Membrane lipids

GENETICS accepted
GENETICS accepted

... chromosome, with lesser effects on the autosomes. him-5 mutants also show a change in crossover distribution on both the X and autosomes. These phenotypes are accompanied by a delayed entry into pachytene and premature desynapsis of the X chromosome. The nondisjunction, progression defects and desyn ...
Alus
Alus

... splits the DNA segment between the guanine and cytosine. ...
Multiple Manner Transposons in Flatworms and Hydras Are Related
Multiple Manner Transposons in Flatworms and Hydras Are Related

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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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