Low chromosome number angiosperms
... suspension culture: karyotype changes with numerical and structural alteration commonly occurs in a tissue culture enviroment. Such alterations lead the the somaclonal variation in regenerated plant or may inhibit the morphogentic potential of cells. Singh (1981) investigated the origin of aneuploid ...
... suspension culture: karyotype changes with numerical and structural alteration commonly occurs in a tissue culture enviroment. Such alterations lead the the somaclonal variation in regenerated plant or may inhibit the morphogentic potential of cells. Singh (1981) investigated the origin of aneuploid ...
From reads to genes in less than 10 R commands
... – Two libraries from sequencing of Universal Human Reference RNA sample: A_1 and A_2 – Two libraries from sequencing of Human Brain Reference RNA sample: B_1 and B_2 – Reads mapped to Chr 1 were chosen (according to Subread) for this workshop ...
... – Two libraries from sequencing of Universal Human Reference RNA sample: A_1 and A_2 – Two libraries from sequencing of Human Brain Reference RNA sample: B_1 and B_2 – Reads mapped to Chr 1 were chosen (according to Subread) for this workshop ...
Two-Component System of Mycobacterium Tuberculosis
... the histidine protein kinase (HK) PhoR, are involved in the induction of Pho-regulon genes including the phoPR operon and genes encoding the major vegetative alkaline phosphatases, phoA and phoB. The PhoP protein belongs to OmpR/PhoB subfamily, which is considered as the largest of the response regu ...
... the histidine protein kinase (HK) PhoR, are involved in the induction of Pho-regulon genes including the phoPR operon and genes encoding the major vegetative alkaline phosphatases, phoA and phoB. The PhoP protein belongs to OmpR/PhoB subfamily, which is considered as the largest of the response regu ...
Identification of eight novel coagulation factor XIII subunit A mutations
... structure of the factor XIIIA protein. Future in vitro expression studies of the factor XIII mutations are required to confirm their pathological mechanisms. Key words: factor XIII deficiency, FXIII-A, FXIII-B, structural analysis. ...
... structure of the factor XIIIA protein. Future in vitro expression studies of the factor XIII mutations are required to confirm their pathological mechanisms. Key words: factor XIII deficiency, FXIII-A, FXIII-B, structural analysis. ...
Pig genetics: insight in minipigs
... Genetics in the Czech Republic because of the similarities between human and porcine skin (Zhang and Monteiro-Rivière, 1997). Initially, an inheritable form of cutaneous melanoma has been described in the Hormel miniature pig strain. A strain of Hormel miniature pigs, known as Sinclair pigs were fou ...
... Genetics in the Czech Republic because of the similarities between human and porcine skin (Zhang and Monteiro-Rivière, 1997). Initially, an inheritable form of cutaneous melanoma has been described in the Hormel miniature pig strain. A strain of Hormel miniature pigs, known as Sinclair pigs were fou ...
RNA Amplification and cDNA Synthesis for qRT
... transcription, the samples were treated with DNase I to remove the ds cDNA template, as well as a large portion of genomic DNA. The amplified RNA was purified using an RNA Clean & Concentrator™-5 column (Zymo Research) and then reverse-transcribed, using random primers, into cDNA in a final volume o ...
... transcription, the samples were treated with DNase I to remove the ds cDNA template, as well as a large portion of genomic DNA. The amplified RNA was purified using an RNA Clean & Concentrator™-5 column (Zymo Research) and then reverse-transcribed, using random primers, into cDNA in a final volume o ...
#2
... compared to that obtained with GC* (r2 ¼ 0.61), and it is entirely due to the relationship between GC and GC* (figs. 2 and 3). This clearly shows that it is not the base composition that affects recombination rate, but instead that recombination drives the evolution of base composition. These result ...
... compared to that obtained with GC* (r2 ¼ 0.61), and it is entirely due to the relationship between GC and GC* (figs. 2 and 3). This clearly shows that it is not the base composition that affects recombination rate, but instead that recombination drives the evolution of base composition. These result ...
MERE1, a Low-Copy-Number Copia-Type
... However, a polymorphism is detected between the Jemalong and R108 lines. Similarly, using this technique, a polymorphism can be detected between the different lines from a small M. truncatula ecotype collection (Fig. 4C), despite a rather constant copy number of the element in these different lines. ...
... However, a polymorphism is detected between the Jemalong and R108 lines. Similarly, using this technique, a polymorphism can be detected between the different lines from a small M. truncatula ecotype collection (Fig. 4C), despite a rather constant copy number of the element in these different lines. ...
Solid phase peptide synthesis (SPPS), strategies, resins and
... temporary and permanent (side chain) protecting groups are both acid labile side chain deprotection during repeated TFA treatment can occur repeated TFA-mediated N-α-deprotection over the course of a long synthesis may lead to modification and/or degradation of sensitive peptide sequences di ...
... temporary and permanent (side chain) protecting groups are both acid labile side chain deprotection during repeated TFA treatment can occur repeated TFA-mediated N-α-deprotection over the course of a long synthesis may lead to modification and/or degradation of sensitive peptide sequences di ...
PowerPoint Slides - Evo-Ed
... Funding for the Evo-Ed Project is provided by the National Science Foundation and by Lyman Briggs College, Michigan State University. These slides are provided as a teaching resource. You are encouraged to modify them to meet your specific teaching and learning needs. Please adhere to the copyright ...
... Funding for the Evo-Ed Project is provided by the National Science Foundation and by Lyman Briggs College, Michigan State University. These slides are provided as a teaching resource. You are encouraged to modify them to meet your specific teaching and learning needs. Please adhere to the copyright ...
Fetal Hemoglobin Levels in Sickle Cell Disease and
... F-cell assays. Assays for percent F cells were performed on peripheral blood to determine F-cell production in nonanemic normal (Hb AA) and AS subjects. Monoclonal mouse anti-human Hb F antibody was used to enumerate lo4 RBCs per assay using a flow cytometry technique.z3 An assay for the percent of ...
... F-cell assays. Assays for percent F cells were performed on peripheral blood to determine F-cell production in nonanemic normal (Hb AA) and AS subjects. Monoclonal mouse anti-human Hb F antibody was used to enumerate lo4 RBCs per assay using a flow cytometry technique.z3 An assay for the percent of ...
Maritimibacter alkaliphilus gen. nov., sp. nov., a genome
... System (MIDI), by the Center. The estimated genome size, based on genome sequencing data, was approximately 4.5 mbp, coding for 4757 open reading frames. The DNA G+C content of strain HTCC2654T was 64.1 mol% from genome sequences and 61.7 mol% with the HPLC method. The only respiratory quinone detec ...
... System (MIDI), by the Center. The estimated genome size, based on genome sequencing data, was approximately 4.5 mbp, coding for 4757 open reading frames. The DNA G+C content of strain HTCC2654T was 64.1 mol% from genome sequences and 61.7 mol% with the HPLC method. The only respiratory quinone detec ...
Bioinformatik - Chair of Computational Biology
... Enzymes that catalyze more than one reaction Genome predictions usually assign a single enzymatic function. However, E.coli is known to contain many multifunctional enzymes. Of the 607 E.coli enzymes, 100 are multifunctional, either having the same active site and different substrate specificities ...
... Enzymes that catalyze more than one reaction Genome predictions usually assign a single enzymatic function. However, E.coli is known to contain many multifunctional enzymes. Of the 607 E.coli enzymes, 100 are multifunctional, either having the same active site and different substrate specificities ...
Lesson Overview
... bridge forms between two bacterial cells, genetic material, usually in the form of a plasmid, moves from one cell to the other. This transfer of genetic information increases genetic diversity Many plasmids carry genes that enable bacteria to survive in new environments or to resist antibiotics that ...
... bridge forms between two bacterial cells, genetic material, usually in the form of a plasmid, moves from one cell to the other. This transfer of genetic information increases genetic diversity Many plasmids carry genes that enable bacteria to survive in new environments or to resist antibiotics that ...
Better than Chance: the importance of null models
... Is residue-residue contact prediction better than chance? Early predictors (1994) reported results that were 1.4 to 5.1 times “better than chance” on a sample of 11 proteins. But they used a uniform null model: P(residue i contacts residue j) = constant . A better null model: P (residue i contacts r ...
... Is residue-residue contact prediction better than chance? Early predictors (1994) reported results that were 1.4 to 5.1 times “better than chance” on a sample of 11 proteins. But they used a uniform null model: P(residue i contacts residue j) = constant . A better null model: P (residue i contacts r ...
Signal Peptide-Selection of cDNA Cloned Directly from the
... the partial cDNA sequences were not available, full-length sequences need to be obtained to further analyze the potential subcellular localization and database homology of each predicted peptide. In situ localization of genes expressed within specimens of H. glycines. The digoxygenin-11-dUTP (DIG)-l ...
... the partial cDNA sequences were not available, full-length sequences need to be obtained to further analyze the potential subcellular localization and database homology of each predicted peptide. In situ localization of genes expressed within specimens of H. glycines. The digoxygenin-11-dUTP (DIG)-l ...
BLOTTING.142
... Size-separation of the fragments (standard agarose gel electrophoresis) In situ denaturation of the DNA fragments (by incubation @ ↑temp) Transfer of denatured DNA fragments into a solid support (nylon or ...
... Size-separation of the fragments (standard agarose gel electrophoresis) In situ denaturation of the DNA fragments (by incubation @ ↑temp) Transfer of denatured DNA fragments into a solid support (nylon or ...
Genetic Algorithms and Evolutionary Strategies 1
... 3. Fitness ranking: To avoid using extreme fitness values, sort individuals according to raw fitness, then assign reproductive trials according to rank (found to be superior to scaling) 4. Tournament selection: Select a pair of individuals from the population at random (with replacement), copy the b ...
... 3. Fitness ranking: To avoid using extreme fitness values, sort individuals according to raw fitness, then assign reproductive trials according to rank (found to be superior to scaling) 4. Tournament selection: Select a pair of individuals from the population at random (with replacement), copy the b ...
MYH Associated Polyposis (MAP)
... in size. There are different types of polyps and those most commonly found in MAP are called adenomas. Most people with MAP have between 10 and 500 polyps. Polyps caused by MAP do not usually appear until adulthood and are less numerous than those found in patients with another genetic condition cal ...
... in size. There are different types of polyps and those most commonly found in MAP are called adenomas. Most people with MAP have between 10 and 500 polyps. Polyps caused by MAP do not usually appear until adulthood and are less numerous than those found in patients with another genetic condition cal ...
Oligogenic basis of isolated gonadotropin
... in more than one locus in each affected individual. Elucidating the principles of oligogenic inheritance and mechanisms of genetic interactions could help unravel the newly appreciated role of rare sequence variants in polygenic disorders. With few exceptions, however, the precise genetic architectu ...
... in more than one locus in each affected individual. Elucidating the principles of oligogenic inheritance and mechanisms of genetic interactions could help unravel the newly appreciated role of rare sequence variants in polygenic disorders. With few exceptions, however, the precise genetic architectu ...
Problem Set 1
... b. Output the full oligo sequence and its GC content to the screen (5 points). 2. Calculate Tm (described in the skeleton code) for a test 70-mer. (5 points) 3. Read in the SARS genome and parse through all possible 70-mers, calculate GC content and Tm for each 70-mer in the SARS genome, filter out ...
... b. Output the full oligo sequence and its GC content to the screen (5 points). 2. Calculate Tm (described in the skeleton code) for a test 70-mer. (5 points) 3. Read in the SARS genome and parse through all possible 70-mers, calculate GC content and Tm for each 70-mer in the SARS genome, filter out ...