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Nucleic acid vaccines against rickettsial diseases and methods of use
Nucleic acid vaccines against rickettsial diseases and methods of use

... against R. rickettsii (VishWanath, S., G. McDonald, N. Watkins [1990] Infect. Immun. 581646). It is knoWn that there is structural variation in rickettsial antigens betWeen different geographical isolates. Thus, a functional recombi nant vaccine against multiple isolates Would need to contain ...
Transgenic Rice Plants Containing a Chlorsulfuron Resistance
Transgenic Rice Plants Containing a Chlorsulfuron Resistance

Respiration Worksheet
Respiration Worksheet

... J. Multiple Alleles So far, all genes have been coded for by two alleles, one from the father and one from the mother. This will always be the case as each parent can only donate one allele. Certain genes are coded for by more than two alleles, so the phenotype depends on which alleles are passed do ...
LP - Columbia University
LP - Columbia University

... What happens in an individual meiosis depends on whether the total # of crossovers is even or odd and which chromatids are involved For more details, see the second diagram of lecture 22, topic VI-B, called "multiple crossovers." This diagram is included FYI only. Important note: The diagrams referr ...
Mouse models of obesity
Mouse models of obesity

Evaluation of the role of rank and opg genes in paget`s disease of
Evaluation of the role of rank and opg genes in paget`s disease of

... Paget’s disease of bone (PDB) has an estimated prevalence up to 5% in the population over the age of 55 years,2,11 and is thereby the second leading skeletal disorder affecting the older population of the western world, exceeded only by osteoporosis. The disease involves expedited remodeling, result ...
Comparison of conserved structural and regulatory domains within
Comparison of conserved structural and regulatory domains within

... rRNA and 23S rRNA genes of the cyanobacterium Nostoc PCC 7120 gave three products. Two represented true ITS regions of different sizes, while the third was a heteroduplex. The longer spacer (ITS-L) contained 512 nucleotides and carried tRNAIle and tRNAAla genes, separated by a large stem–loop struct ...
The RNA origin of transfer RNA aminoacylation and beyond
The RNA origin of transfer RNA aminoacylation and beyond

... events could have been catalysed by RNA alone in the RNA world. However, tRNA aminoacylation, another essential reaction of translation, is solely catalysed by protein enzymes, aminoacyl-tRNA synthetases (ARSs), in the modern system. In the RNA world hypothesis, this discrepancy could be explained b ...
Maintenance of DNA Methylation during the Arabidopsis Life Cycle
Maintenance of DNA Methylation during the Arabidopsis Life Cycle

... FIS2 maternal allele in the endosperm. A putative general paternal silencing has been observed for reporters of endospermexpressed genes for up to 4 DAP (Vielle-Calzada et al., 2000). It could be hypothesized that such a general mechanism also affects expression at genomic loci for genes expressed i ...
Detection of mcr-1 colistin resistance gene in
Detection of mcr-1 colistin resistance gene in

werribee secondary college vce unit planner ~ 2004
werribee secondary college vce unit planner ~ 2004

... Homework: SAC1 revision and mitosis, meiosis revision including exam questions Tuesday: Molecular genetics, the nature of the genomes, genes and the genetic code Thursday: The role of RNA in transcription and in translation for Eukaryotes Friday: SAC1: an investigation or simulation related to mitos ...
Nucleic Acids - Lyndhurst Schools
Nucleic Acids - Lyndhurst Schools

... Organic Compounds: Nucleic Acids ...
Lo, Frederick: Three Recently Developed Algorithms for Aligning Distantly Related Proteins
Lo, Frederick: Three Recently Developed Algorithms for Aligning Distantly Related Proteins

... protein pairs from the SCOP database. The pairs had ~45% identity or less. All three methods require an initial alignment and similarity matrix, so the profile-profile alignment algorithm Fold and Function Assignment System (FFAS) developed by the Jaroszewski group was utilized (Rychlewski et al. 20 ...
Volume 11 - Número 6 - Novembro / Dezembro de 2001
Volume 11 - Número 6 - Novembro / Dezembro de 2001

... vicarious calcification, and transient facial palsy. Autism has been reported. The molecular defect underlying Williams syndrome is a large deletion at chromosomal band 7q, encompassing 1.5-2.0 MB of genomic DNA and including the complete elastin gene(19). In addition, this region contains other 14 ...
BGS 118, Low number of tillers 1, lnt1
BGS 118, Low number of tillers 1, lnt1

... The tiller number is reduced to 2 to 4 per plant. These tillers are formed soon after seedling emergence; hence, no lateemerging tillers are observed. Culms are thick and stiff and leaves are dark green (7). Plants mutants at the lnt1 locus fail to produce secondary tillers (1). Occasional spike mal ...
CMBI
CMBI

The genus Listonella MacDonell and Colwell 1986 is a later
The genus Listonella MacDonell and Colwell 1986 is a later

View - Plos
View - Plos

... spread in phytophagous beetles, we pyrosequenced (454, Roche) cDNA libraries generated from five species of beetles, including the one from C. tremulae [18]. First we assessed the potential level of contamination of our EST datasets by eukaryotic organisms present in the gut flora or being potential ...
Chapter 12
Chapter 12

... • β-galactosides, the substrates of the lac operon, are its inducer. ...
domaination
domaination

... multidomain proteins. Not a surprise since: – Structural domain boundaries will not always coincide with sequence domain boundaries – Proteins must have some domain shuffling ...
Rapid Translation System RTS 500 E. coli HY Kit
Rapid Translation System RTS 500 E. coli HY Kit

... fact, this methodology only involves liquid handling and can thus be adapted to automated processes. Low yield has been the limiting factor for many years in cell-free protein expression making this method suitable only as an analytical tool. The development of the RTS 500 E. coli Circular Template ...
Chapter 5 Macromolecules
Chapter 5 Macromolecules

... • When phospholipids are added to water, they self-assemble into a bilayer, with the hydrophobic tails pointing toward the interior • The structure of phospholipids results in a bilayer arrangement found in cell membranes • Phospholipids are the major component of all ...
the lysis/lysogeny switch in phage \lambda
the lysis/lysogeny switch in phage \lambda

... Question: How does the phage switch from one state to the other? ...
Exercise Controls Gene Expression
Exercise Controls Gene Expression

... after one to two hours of recovery. That work, as well as several similar studies, further revealed that exercise transiently activates transcription of a large number of other mitochondrial genes but that the degree of activation is much smaller, increasing only two- or threefold (see Figure 3). Th ...
S-B-9-1_Rabbit Natural Selection Laboratory Activity
S-B-9-1_Rabbit Natural Selection Laboratory Activity

... 5. Shake the bag to simulate the rabbits mating. Without looking, select two alleles at a time. Place the rabbits (pairs of alleles) in the appropriate dish: FF, Ff, or ff. Record the results in the data table next to “Generation 1.” For example, if you draw colors represented by Ff, place a tally m ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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