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Atomic force microscopy studies of the molecular mechanisms of
Atomic force microscopy studies of the molecular mechanisms of

... of the DNA between RNAPs causes them to stall or pause when they get too close to one another. This may be one fundamental way that the cell controls gene expression through the physical properties of the DNA, but more work is needed to prove the hypothesis and understand the details. It is being in ...
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Location of Genes_Gene Expression
Location of Genes_Gene Expression

... • Gene regulation can occur at any point during gene expression, but most commonly occurs at the level of transcription – the information in a gene’s DNA is transferred to mRNA – genetic information always goes from DNA to RNA to protein – a given cell only transcribes a specific set of genes and no ...
What is Cloning?
What is Cloning?

... Cloning DNA in Plasmids  By fragmenting DNA of any origin (human, animal, or plant) and inserting it in the DNA of rapidly reproducing foreign cells, billions of copies of a single gene or DNA segment can be produced in a very short time. DNA to be cloned is inserted into a plasmid (a small, self ...
Thomas Geburek - WordPress.com
Thomas Geburek - WordPress.com

... Sweet genes: Do we shape the gene pool of our forests ? ...
Big Idea #3
Big Idea #3

... STRUCTURE OF DNA  DNA is a polymer made of nucleotide monomers  Each nucleotide is made of a phosphate group attached to a five carbon sugar and one of four possible nitrogen bases: A ,T,C, or G. Sugar in DNA is deoxyribose Sugar in RNA is ribose Bases are divided into purines (A,G) and pyrimidin ...
Genetically Modified Food
Genetically Modified Food

... Mechanism1 : Isolating/synthesizing the target gene  The ‘shotgun’ approach, using type II restriction enzyme(restriction endonuclease ) - the enzyme cuts at recognition sites, to obtain a desired gene - sticky ends or blunt ends produced (there are figures later)  Making a copy of the gene from ...
Structure I: DNA to RNA to Protein
Structure I: DNA to RNA to Protein

... • Each protein has a unique sequence of amino acids • The sequence of amino acids specifies protein shape and function ...
Chapter Three The Biological Basis of Life
Chapter Three The Biological Basis of Life

... second tRNA–amino acid complex arrives at the ribosome. ...
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... Genes serves as a recipe for building a protein molecule (these are called “structural genes”) When a particular protein is needed by the cell, the corresponding gene, made of DNA, is turned "on," or transcribed into messenger RNA, which then carries the "protein recipe" to the proteinmaking machine ...
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... A cholesterol-carrying particle in the blood, made up of Lipoprotein) cholesterol and other lipids surrounded by a single layer of phospholipids in which proteins are embedded. An LDL particle carries more cholesterol than a related lipoprotein, HDL, and high LDL levels in the blood correlate with a ...
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... Green – introns (non-coding regions, which will be spliced out of the mRNA) Unlike in yeasts, in higher eukaryotes genomic DNA does not represent continuous coding regions, thus spliced mRNA is usually taken as a starting material ...
Unit 9 Completed Vocabulary - WAHS
Unit 9 Completed Vocabulary - WAHS

... transformation – process in which one strain of bacteria is changed by a gene or genes from another strain of bacteria. bacteriophage – a virus that infects bacteria. nucleotide – monomer of nucleic acids made up of a 5-carbon sugar, a phosphate group, and a nitrogenous base. base pairing – principl ...
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... B. Random insertion of the targeting vector. The cells are NeoR but will not grow on gancyclovir since TK is also inserted and that converts gancyclovir into a cell-toxic product. ...
DNA Workshop - Lapeer High School
DNA Workshop - Lapeer High School

... Follow the directions and answer the questions as you go along. First click the button in the upper left that says “DNA Replication.” Follow the prompts and go through the animation. You can repeat if necessary. a. What kind of protein unzips the DNA to start the process? b. Which bases always pair ...
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Bio 1 Unit Objectives Protein Synthesis Readings

... Exploring Life: Chapter 11 Online Activities ...
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... One of several alternative forms of a gene that occupies a given locus on a chromosome. Alleles can differ from one another in their phenotypic effects. At the molecular level, alleles differ from one another based on their nucleotide sequences, regardless of their effect on phenotype. ...
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DNA - Valhalla High School

... cell must make another copy of, or replicate it’s DNA.  The DNA molecule basically unzips itself by breaking the hydrogen bonds holding the two strands of nucleotides together.  Each strand then forms a second strand by using free nucleotides which are found in the nucleus.  DNA replication simpl ...
Classical Genetics
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... Prim rose). Gene mutations (Point mutations) affect the genes. It may be Deletion (removal of bases), Addition or Insertion and Substitution (replacement of one base by another one). Substitution may be Transition (Purine-Purine or Pyrimidine-Pyrimidine change) or Tranversion (PurinePyrimidine chang ...
Microbiology - Las Positas College
Microbiology - Las Positas College

... Identify the roles of a clone and a vector in making recombined DNA. Compare selection and mutation. Define REs, and outline their use to make recombinant DNA. List some properties of vectors and describe their use. Outline the steps in PCR and provide an examples of its use. Describe various differ ...
Select one of your Biology instructors from another class and look
Select one of your Biology instructors from another class and look

... three adjacent nucleotides will be a start codon? A stop codon? In an mRNA molecule of random sequence, what is the average distance between stop codons? 8.2 If DNA consisted of only two nucleotides (say, A and T) in any sequence, what is the minimum number of adjacent nucleotides that would be need ...
Molecular Biology Primer
Molecular Biology Primer

... specific set of approximately 13 nucleotides marking the beginning of genes – 1 nucleotide that serves as a transcriptional start site – 6 that are 10 nucleotides 5' to the start site, and – 6 more that are 35 nucleotides 5' to the start site – What is the frequency for the sequence to occur? ...
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Genetic Technology

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genetics review package
genetics review package

... barcode or signature. This can be used to prove identity of individuals and to prove paternity. 9. What is DNA amplification and why is it used? DNA amplification is the process where DNA is replicated for therapeutic, cloning or investigative purposes. Enzymes and heat are added in alteration to pr ...
Extra Credit DNA Study Guide
Extra Credit DNA Study Guide

... 4. Use a restriction enzyme to cut the insulin gene out of the human DNA. 5. Use a restriction enzyme to cut the bacterial plasmid round DNA. 6. Insert the genetic marker and the gene into the plasmid DNA. 54. What is the process illustrated on page 304-305? ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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