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1.d Standard curve construction and validation of the C t
1.d Standard curve construction and validation of the C t

File - Ms. Wilson`s Biology Class
File - Ms. Wilson`s Biology Class

... Read the text below and answer the following questions: 1. In order to speed up the copying process (replication), DNA replication begins at ___________ locations along each chromosome. 2. The two DNA strands are pulled apart and copied in both directions at the rate of about _________ nucleotides p ...
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Information Flow 2

... The mRNA that is first transcribed is much longer than the mRNA that will eventually be translated. Eukaryotic genes commonly have intervening sequences that must be removed before an mRNA that codes for the proper sequence of amino acids can be created. The intervening sequences that must be remove ...
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NSDTR Degenerative Encephalopathy

... Recently, we have identified a new brain disease in Nova Scotia Duck Tolling Retrievers. The purpose of this article is to provide information about the condition so that breeders and veterinarians can be alert to any future cases and help us find the gene responsible. What is NSDTR Degenerative Enc ...
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Isolation and Comparative Genomic Analysis of Final Third of Satis

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... 6) If a substitution occurred to the 6th base in the DNA template strand, such that cytosine was changed to thymine, would the final protein change? Why? No. Initially, the DNA strand had the triplet TTC – this created the mRNA codon AAG. If we change the template to TTT, the new codon would be AAA. ...
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Chapter 11 How Genes are Controlled

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DNA replication to translation

... two directional polynucleotide strands in double helix nucleotides are linked in chains with a phosphodiester bond free ends of chain will have 5’ phosphate at one end, 3’ hydroxyl at the other end 5’ end ...
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... form. Serine residue 100 is the major site of TAL2 phosphorylation in vivo. And it serves as an effective in vitro substrate for MAP kinases such as ERK1. TAL2 polypeptides interact in vivo with the E2A gene products to form HLH heterodimers that bind DNA, the result is the E2A inactivation. The E2A ...
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... • 35,000 ~ 40,000 genes with multiple splicing products per gene (build 34). • Finish at April, 2003 & single chromosome papers published one by one. • The entire human genome was finished again Oct. 2004. Build 35 assembly with 2.85 billion nucleotides interrupted by only 341 gaps. It covers 99% of ...
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... uracil biosynthetic machinery. It will therefore kill a URA3 cell. It will not kill a ura3 cell, since an enzyme required to turn 5-FOA into a toxic chemical is missing. Using this drug, you can plate a mixture of a million URA3 cells and one ura3 cell on a plate containing 5-FOA and only the single ...
probability and genetics
probability and genetics

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DNA Computing on a Chip

...  Complementary DNA strands that satisfy the first clauses are added to the solution.  The remaining single strands are destroyed by enzymes.  The surface is then heated to melt away the complementary strands.  This cycle is repeated for each of the remaining clauses. ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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