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Fulltext - Jultika
Fulltext - Jultika

... Biochemistry, University of Oulu, and Biocenter Oulu, Finland, and at the Center for Gene Therapy, the late Allegheny University of the Health Sciences, Philadelphia, USA, during the last years of the second millenium Anno Domini. I wish to express my sincere thanks to my supervisor, Docent Leena Al ...
"Nitrogen Metabolism". In: Microbial Physiology (Fourth Edition)
"Nitrogen Metabolism". In: Microbial Physiology (Fourth Edition)

... nitrogenase, nitrogenase 3, is encoded by alternate nitrogen fixation (anfHDK ) genes, which are expressed in A. vinelandii only in the absence of Mo and V. Dinitrogenase reductase 3 contains two identical subunits and dinitrogenase 3 is present in two active configurations, α2 β2 and α1 β2 . The re ...
25_2
25_2

... complex DNA such as the human genome from a very small amount of DNA solution [1]. Many PCR machines are available on the market. Recently, micro PCR which can amplify the sequence faster using less amounts of reagents conveniently than the conventional PCR machines are taking the center stage [2-5] ...
Comparative analysis of two-component signal transduction systems
Comparative analysis of two-component signal transduction systems

alignable - gobics.de: Department of Bioinformatics
alignable - gobics.de: Department of Bioinformatics

... For each position x and each sequence Si exist an upper bound ub(x,i) and a lower bound lb(x,i) for residues y in Si that are alignable with x ...
End-product control of enzymes of branched
End-product control of enzymes of branched

... isoleucine-valine pathway, the combination of all three amino acids gave in individual experiments activities 0.12-0.19 of those with no supplementation, the ratio of the means being 0.16. Supplementation with threonine, or with leucine, isoleucine or valine singly or in pairwise combinations, gave ...
genetics - Liceocopernico.it
genetics - Liceocopernico.it

... ability to exchange genetic material between a wide range of unrelated organisms. Medicine and agriculture may literally be revolutionized by these Tecent developments in molecular genetics. Some exposure to college-level or university-level biology is desirable before embarking on the study of gene ...
mtr function Background Luis M. Corrochano
mtr function Background Luis M. Corrochano

... spreading 0.2 ml of conidial suspension on the surface of the selective agar or by use of spreading agar with the appropriate supplements. The handling and plating of conidia should be performed in darkness or dim light to prevent photorepair. The plates should be incubated in the dark, at least for ...
Cellecta Technical Note - RNAi Screen - PUMA
Cellecta Technical Note - RNAi Screen - PUMA

... we could use high-throughput (HT) sequencing to address both of these points. First, we looked at the library. When the library itself was hybridized to the Affymetrix HG U133+2 arrays, only approximately half the shRNA sequences were detectable. This may be a result of weak hybridization for this s ...
Enzyme Mechanisms - Illinois Institute of Technology
Enzyme Mechanisms - Illinois Institute of Technology

... Incorporates DNA replication as an analytical tool for determining sequence Uses short primer that attaches to the 3’ end of the ssDNA, after which a specially engineered DNA polymerase Each vial includes one dideoxyXTP and 3 ordinary dXTPs; the dideoxyXTP will be incorporated but will halt synthesi ...
Genbiotech Store
Genbiotech Store

Light affects motility and infectivity of Agrobacterium tumefaciens
Light affects motility and infectivity of Agrobacterium tumefaciens

Obesity — a genetic disease of adipose tissue?
Obesity — a genetic disease of adipose tissue?

Creation/Evolution - Geoscience Research Institute
Creation/Evolution - Geoscience Research Institute

... representing a few amino acids or possibly one codon representing a “group” of amino acids More precise codon meaning evolves perhaps with only the first two bases having meaning with discrimination at the third position evolving later The code becomes “frozen” when the system becomes so complex tha ...
Document
Document

... blood type classification system is the ABO (say "AB-O") system discovered by Karl Landsteiner in the early 1900s. There are four types of blood in the ABO system: A, B, AB, and O. Your blood type is established before you are born, by specific genes inherited from your parents. You receive one gene ...
A Review of using Spray Pyrolysis through Sol-gel
A Review of using Spray Pyrolysis through Sol-gel

Triphosphatase Related to the Protein Tyrosine Phosphatases
Triphosphatase Related to the Protein Tyrosine Phosphatases

... It remains to be seen whether the CEL-1 triphosphatase is representative of all higher eukaryotic capping enzymes. Biochemical characterizations of capping enzymes from two higher eukaryotes (brine shrimp and rat) are consistent with a single protein with both triphosphatase and guanylyltransferase ...
Dr. Stephanie Sherman Fragile X Research
Dr. Stephanie Sherman Fragile X Research

The bonobo genome compared with the chimpanzee and human
The bonobo genome compared with the chimpanzee and human

... closely related to chimpanzees. Moreover, comparison of a small number of autosomal DNA sequences has shown that bonobo DNA sequences often fall within the variation of chimpanzees5. Bonobos and chimpanzees are highly similar to each other in many respects. However, the behaviour of the two species ...
Chapter 3 Transposon Mutagenesis of Rhodobacter sphaeroides
Chapter 3 Transposon Mutagenesis of Rhodobacter sphaeroides

... procedure damages the target DNA and sometimes, during repair, an improper base pair (or pairs) is incorporated in the DNA, causing a mutation. Chemical mutagens are also employed. These compounds are added to a growing culture of an organism for a given time period and interfere with the replicatio ...
Knox, Kirstin : An Introduction To Motif Based Functional Classification of Large Protein Families
Knox, Kirstin : An Introduction To Motif Based Functional Classification of Large Protein Families

... One major challenge is to develop effective methods for assigning specific functions to proteins within large families and for classifying family members into groups that share functional characteristics. While multiple sequence alignments can be incredibly useful in predicting functionally or struc ...
The novel genome organization of the insect picorna
The novel genome organization of the insect picorna

... 3« terminus of the RNA with an oligo(dT) primer. Further cDNA synthesis was initiated with oligonucleotide primers designed according to sequences toward the end of the preceding clone. In each case, second strand synthesis was performed using RNase H and E. coli polymerase I (Promega). The cDNA was ...
Identification of a Vinyl Reductase Gene for
Identification of a Vinyl Reductase Gene for

... CIW8. We identified 38 plants that exhibited a recombination in this interval. New single nucleotide polymorphism markers were generated from the sequence information from the Monsant Arabidopsis polymorphism collection database, and we confirmed that the DVR gene is located on the region covered by ...
A RARE KEL17/KEL(IVS3+1G>A) COMPOUND HETEROZYGOUS
A RARE KEL17/KEL(IVS3+1G>A) COMPOUND HETEROZYGOUS

... Background: The Kell blood group system includes some of the most immunogenic antigens among blood a a groups know. Among them Kell(KEL1), Kp (KEL3), and Js (KEL6) are well known. The antithetic antigens KEL11/17 further contribute to this list. However, KEL17 is considered as very rare, with an app ...
Sequence comparison of aflR from different Aspergillus species
Sequence comparison of aflR from different Aspergillus species

... called the S strain of A. flavus (Cotty, 1989) or A. flavus var. parvisclerotigenus (Saito and Tsuruta, 1993), while the latter has been called the L strain of A. flavus (Cotty, 1989). Atypical A. flavus isolates which produce both B and G aflatoxins were also included. These were described previously as ...
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Artificial gene synthesis

Artificial gene synthesis is a method in synthetic biology that is used to create artificial genes in the laboratory. Currently based on solid-phase DNA synthesis, it differs from molecular cloning and polymerase chain reaction (PCR) in that the user does not have to begin with preexisting DNA sequences. Therefore, it is possible to make a completely synthetic double-stranded DNA molecule with no apparent limits on either nucleotide sequence or size. The method has been used to generate functional bacterial or yeast chromosomes containing approximately one million base pairs. Recent research also suggests the possibility of creating novel nucleobase pairs in addition to the two base pairs in nature, which could greatly expand the possibility of expanding the genetic code.Synthesis of the first complete gene, a yeast tRNA, was demonstrated by Har Gobind Khorana and coworkers in 1972. Synthesis of the first peptide- and protein-coding genes was performed in the laboratories of Herbert Boyer and Alexander Markham, respectively.Commercial gene synthesis services are now available from numerous companies worldwide, some of which have built their business model around this task. Current gene synthesis approaches are most often based on a combination of organic chemistry and molecular biological techniques and entire genes may be synthesized ""de novo"", without the need for precursor template DNA. Gene synthesis has become an important tool in many fields of recombinant DNA technology including heterologous gene expression, vaccine development, gene therapy and molecular engineering. The synthesis of nucleic acid sequences is often more economical than classical cloning and mutagenesis procedures.
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