Metagenomic analysis of a soil enrichment cultures
... 16s rRNA gene of virtually all species because they hybridize to a highly conserved region. However, an important difference here is that the template is a pool of mixed genomic DNA, so there are potentially 1000s of different 16s rRNA genes present. Each one is amplified in the reaction, yielding a ...
... 16s rRNA gene of virtually all species because they hybridize to a highly conserved region. However, an important difference here is that the template is a pool of mixed genomic DNA, so there are potentially 1000s of different 16s rRNA genes present. Each one is amplified in the reaction, yielding a ...
Frederick Sanger - Nobel Lecture
... were two residues of each assuming a molecular weight of 12,000. This suggested to us that insulin was composed of four polypeptide chains, two with phenylalanine and two with glycine end-groups. This method has now been applied widely to many proteins and peptides, and together with the Edman pheny ...
... were two residues of each assuming a molecular weight of 12,000. This suggested to us that insulin was composed of four polypeptide chains, two with phenylalanine and two with glycine end-groups. This method has now been applied widely to many proteins and peptides, and together with the Edman pheny ...
gene cloning and identification of the Circumsporozoite protein of
... The sporozoite stage of malaria parasites carries a protein on its outer surface (1) which expresses a unique immunodominant epitope recognized by immunized or repeatedly infected hosts (33, 34). Sera from mice immunized with Plasmodium berghei sporozoites immunoprecipitate a single 44,O000Mr protei ...
... The sporozoite stage of malaria parasites carries a protein on its outer surface (1) which expresses a unique immunodominant epitope recognized by immunized or repeatedly infected hosts (33, 34). Sera from mice immunized with Plasmodium berghei sporozoites immunoprecipitate a single 44,O000Mr protei ...
Comparing Kernels For Predicting Protein Binding Sites From Amino
... protein-DNA, and protein-RNA interface sites from amino acid sequences. Yan et al. [Yan et al., 2004a], [Yan et al., 2004b] have used SVM for identifying protein-protein interface residues among surface residues using amino acid sequence information. Sen et al. [Sen et al., 2004] have proposed an ap ...
... protein-DNA, and protein-RNA interface sites from amino acid sequences. Yan et al. [Yan et al., 2004a], [Yan et al., 2004b] have used SVM for identifying protein-protein interface residues among surface residues using amino acid sequence information. Sen et al. [Sen et al., 2004] have proposed an ap ...
Chapter 30: Protein Synthesis
... Peptide Chain Termination Requires a GProtein Family Member • Elongation continues until the 70S ribosome encounters a “stop” codon • Release factors recognize the stop codon at the A site • Presence of release factors at A site transforms the peptidyl transferase into a hydrolase, which cleaves th ...
... Peptide Chain Termination Requires a GProtein Family Member • Elongation continues until the 70S ribosome encounters a “stop” codon • Release factors recognize the stop codon at the A site • Presence of release factors at A site transforms the peptidyl transferase into a hydrolase, which cleaves th ...
Protein Similarity Score - Santa Clara Law Digital Commons
... three-dimensional structure, with various functional groups of the amino acids positioned in a manner that enable the protein to perform its function. 8 It is the protein's sequence that dictates the threedimensional structure, and hence the functionality of the protein. 9 However, there is some red ...
... three-dimensional structure, with various functional groups of the amino acids positioned in a manner that enable the protein to perform its function. 8 It is the protein's sequence that dictates the threedimensional structure, and hence the functionality of the protein. 9 However, there is some red ...
Determination and Quantification of Molecular Interactions in Protein
... Wheat gluten (WG) is the byproduct, elastic mass after starch is washed from wheat flour dough. WG is an excellent film forming agent, but an addition of plasticizer to the film is necessary to form a homogenous film. Glutenins, gliadins, and the low molecular weight proteins albumins and globulins ...
... Wheat gluten (WG) is the byproduct, elastic mass after starch is washed from wheat flour dough. WG is an excellent film forming agent, but an addition of plasticizer to the film is necessary to form a homogenous film. Glutenins, gliadins, and the low molecular weight proteins albumins and globulins ...
CRACKING THE GENETIC CODE
... components and a method to detect tRNA binding to the ribosome. To isolate the complex he exploited the ability of nylon filters to bind large RNA molecules, such as ribosomes, but not the smaller tRNA molecules. He used a nylon filter to separate ribosomes (and anything bound to the ribosomes) from ...
... components and a method to detect tRNA binding to the ribosome. To isolate the complex he exploited the ability of nylon filters to bind large RNA molecules, such as ribosomes, but not the smaller tRNA molecules. He used a nylon filter to separate ribosomes (and anything bound to the ribosomes) from ...
Mutational effects on protein structure and function Jonas Carlsson Link¨
... but not completely characterised functions. The techniques used are general as demonstrated by applications on metabolic proteins (CYP21, CYP11B1, IAPP, ADH3), regulatory proteins (p53, GDNF) and a transporter protein (ANTR1). When the protein CYP21 (steroid 21-hydroxylase) is deficient it causes CA ...
... but not completely characterised functions. The techniques used are general as demonstrated by applications on metabolic proteins (CYP21, CYP11B1, IAPP, ADH3), regulatory proteins (p53, GDNF) and a transporter protein (ANTR1). When the protein CYP21 (steroid 21-hydroxylase) is deficient it causes CA ...
Rhodopsin
... When active, Transducin’s alpha subunit dissociates from the other two subunits The active Transducin then activates a membrane bound protein called phosphodiesterase Phosphodiesterase hydrolyzes cGMP The hydrolyzes of cGMP, leads to the ion channel closing and the initiation of an action potential ...
... When active, Transducin’s alpha subunit dissociates from the other two subunits The active Transducin then activates a membrane bound protein called phosphodiesterase Phosphodiesterase hydrolyzes cGMP The hydrolyzes of cGMP, leads to the ion channel closing and the initiation of an action potential ...
Cloning, Purification, and Partial Characterization of the
... The Halobacterium sp. NRC-1 MCM gene was amplified using PCR from genomic DNA (kindly provided by Brian Berquist). The PCR reaction was performed with 100 ng genomic DNA, 200 M dNTPs, 5 units of OptimaseTM polymerase (Transgenomic), 1 x Optimase reaction buffer, 1.5 mM MgSO4, 10% DMSO and 0.4 M of ...
... The Halobacterium sp. NRC-1 MCM gene was amplified using PCR from genomic DNA (kindly provided by Brian Berquist). The PCR reaction was performed with 100 ng genomic DNA, 200 M dNTPs, 5 units of OptimaseTM polymerase (Transgenomic), 1 x Optimase reaction buffer, 1.5 mM MgSO4, 10% DMSO and 0.4 M of ...
Calculation of hydrophobicities
... them, instead of precise equality or too tight matching. The adoption of this method depends on the actual result of the calculation of overall errors. 4.3. Weighted errors according to amino acid frequency Another alternative method (Method iii) solves the Eqs. 1, 3, and 5 (i.e. for Methods 1, 2 an ...
... them, instead of precise equality or too tight matching. The adoption of this method depends on the actual result of the calculation of overall errors. 4.3. Weighted errors according to amino acid frequency Another alternative method (Method iii) solves the Eqs. 1, 3, and 5 (i.e. for Methods 1, 2 an ...
Identification of TIpC, a novel 62 kDa MCP
... Expression of TlpC. The 1.9 kb Tag1 fragment containing tlpC was modified by site-directed mutagenesis to create an additional TagI restriction site which was proximally located just upstream of the potential ribosome-binding site. The corresponding 1.7 kb TaqI fragment which was isolated from pDW37 ...
... Expression of TlpC. The 1.9 kb Tag1 fragment containing tlpC was modified by site-directed mutagenesis to create an additional TagI restriction site which was proximally located just upstream of the potential ribosome-binding site. The corresponding 1.7 kb TaqI fragment which was isolated from pDW37 ...
Isolation and Characterization of Plastidic Glucose-6-Phosphate Dehydrogenase
... power and ATP to support fatty acid synthesis in plastids from castor oil seed embryos requires more study of the enzymes involved in the OPPP. ...
... power and ATP to support fatty acid synthesis in plastids from castor oil seed embryos requires more study of the enzymes involved in the OPPP. ...
Table S6: Domains present in the primary network generated from
... This is a bacterial family of uncharacterised proteins. This protein is functionally uncharacterized. It is about 300-500 amino acids in length. This family is found in plants and bacteria. This family of proteins are functionally uncharacterised. This protein is found in bacteria and eukaryotes. Pr ...
... This is a bacterial family of uncharacterised proteins. This protein is functionally uncharacterized. It is about 300-500 amino acids in length. This family is found in plants and bacteria. This family of proteins are functionally uncharacterised. This protein is found in bacteria and eukaryotes. Pr ...
Trends in Plant Science
... SH2 domains have not been found in higher plants. Only two entries (Table 2) encoding an SH2 domain could be predicted from the Arabidopsis genome using programs such as SMART [9]. Although a short segment within the Arabidopsis GAI (Gibberellin Insensitive) gene and its maize and wheat orthologs wa ...
... SH2 domains have not been found in higher plants. Only two entries (Table 2) encoding an SH2 domain could be predicted from the Arabidopsis genome using programs such as SMART [9]. Although a short segment within the Arabidopsis GAI (Gibberellin Insensitive) gene and its maize and wheat orthologs wa ...
The role of histidine residues in low-pH-mediated viral
... simulations were performed in which combinations of these residues were selectively protonated Ragothaman & Kobe [2006–7]. To test the mechanical side-chain function of these histidines, another series of simulations of a triple mutant was performed in which these histidine residues were mutated to ...
... simulations were performed in which combinations of these residues were selectively protonated Ragothaman & Kobe [2006–7]. To test the mechanical side-chain function of these histidines, another series of simulations of a triple mutant was performed in which these histidine residues were mutated to ...
Protein Structure - Research Centers
... The overall 3-D arrangement of atoms in a single polypeptide chain Made up of secondary-structure elements & locally unstructured strands Described in terms of sequence, ...
... The overall 3-D arrangement of atoms in a single polypeptide chain Made up of secondary-structure elements & locally unstructured strands Described in terms of sequence, ...
synthase is regulated by mRNA splicing
... dependent on the activity of the v-src oncogene product pp60V-src, a tyrosine kinase. The genes pp6ov-src induces are also activated by other cell-division stimulators such as the tumor promoter phorbol 12-myristate 13-acetate (PMA), as well as serum and epidermal growth factor (1, 2); however, tran ...
... dependent on the activity of the v-src oncogene product pp60V-src, a tyrosine kinase. The genes pp6ov-src induces are also activated by other cell-division stimulators such as the tumor promoter phorbol 12-myristate 13-acetate (PMA), as well as serum and epidermal growth factor (1, 2); however, tran ...
Additional Methods_Office2004
... both TIR and reflected fluorescence. All imaging was performed using a Nikon 100X 1.49 NA Apo TIRF oil immersion objective and collected over a 40.96 μm × 40.96 μm area onto an Andor iXon 897 EMCCD camera (Andor Technology, UK), with a frame size of 256 × 256 pixels and a corresponding pixel size of ...
... both TIR and reflected fluorescence. All imaging was performed using a Nikon 100X 1.49 NA Apo TIRF oil immersion objective and collected over a 40.96 μm × 40.96 μm area onto an Andor iXon 897 EMCCD camera (Andor Technology, UK), with a frame size of 256 × 256 pixels and a corresponding pixel size of ...
Computational Biology
... 3 phylogenetic profiles for E.coli proteins Proteins with phylogenetic profiles in the neighborhood of ribosomal protein RL7 (A), flagellar structural protein FlgL (B), and histidine biosynthetic protein His5 (C). All proteins with profiles identical to the query proteins are shown in the double bo ...
... 3 phylogenetic profiles for E.coli proteins Proteins with phylogenetic profiles in the neighborhood of ribosomal protein RL7 (A), flagellar structural protein FlgL (B), and histidine biosynthetic protein His5 (C). All proteins with profiles identical to the query proteins are shown in the double bo ...
Innovative Purification Protocol for Heparin Binding
... encoded in the genetic code [12]. There are only 20 standard amino acids that exist in living organism. Sometimes these amino acids are chemically modified in the protein after protein synthesis [11]. In total the number of different proteins, which it is possible to produce from 20 amino acids is ...
... encoded in the genetic code [12]. There are only 20 standard amino acids that exist in living organism. Sometimes these amino acids are chemically modified in the protein after protein synthesis [11]. In total the number of different proteins, which it is possible to produce from 20 amino acids is ...
Marshall Nirenberg - Nobel Lecture
... reported that DNAase inhibited in vitro amino acid incorporation into protein. I had also observed this phenomenon and was greatly interested in it because the results strongly suggested that the cell-free synthesis of protein was dependent, ultimately, upon DNA templates. Heinrich Matthaei then joi ...
... reported that DNAase inhibited in vitro amino acid incorporation into protein. I had also observed this phenomenon and was greatly interested in it because the results strongly suggested that the cell-free synthesis of protein was dependent, ultimately, upon DNA templates. Heinrich Matthaei then joi ...
Probability-Based Scoring Function as a Software
... Bangmod, Thungkru, Bangkok 10140, Thailand; 2BEC Unit, KMUTT-Bangkhuntien, 83 Moo 8, Thakham, Bangkhuntien, Bangkok 10150, Thailand; 3School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkok 10140, Thailand Abstract: One of the major goals of proteomic researc ...
... Bangmod, Thungkru, Bangkok 10140, Thailand; 2BEC Unit, KMUTT-Bangkhuntien, 83 Moo 8, Thakham, Bangkhuntien, Bangkok 10150, Thailand; 3School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkok 10140, Thailand Abstract: One of the major goals of proteomic researc ...
S11. Computational Molecular Modeling- Week 5. 3
... ICM-Browser program and be comfortable helping students make informed choices about what regions of the proteins should be analyzed. I recommend that the instructor performs an internet background search on how protein 3-D modeling works so they understand the various types of resources that are ava ...
... ICM-Browser program and be comfortable helping students make informed choices about what regions of the proteins should be analyzed. I recommend that the instructor performs an internet background search on how protein 3-D modeling works so they understand the various types of resources that are ava ...