"Genetic Methods of Polymer Synthesis". In: Encyclopedia of
... Recombinant DNA methods have been traditionally used in site-directed mutagenesis studies designed to probe protein folding or enzymatic activity. The ease with which genetic sequences can be constructed has, however, led to the increased use of these methods for the synthesis of proteins with repet ...
... Recombinant DNA methods have been traditionally used in site-directed mutagenesis studies designed to probe protein folding or enzymatic activity. The ease with which genetic sequences can be constructed has, however, led to the increased use of these methods for the synthesis of proteins with repet ...
Bioinformatics Toolbox
... routines include spatial plots of microarray data (maimage, redgreencmap), box plots (maboxplot), loglog plots (maloglog), and intensity-ratio plots (mairplot). You can also view clustered expression profiles (clustergram, redgreencmap). You can create 2–D scatter plots of principal components from ...
... routines include spatial plots of microarray data (maimage, redgreencmap), box plots (maboxplot), loglog plots (maloglog), and intensity-ratio plots (mairplot). You can also view clustered expression profiles (clustergram, redgreencmap). You can create 2–D scatter plots of principal components from ...
“Nice” plotting of proteins
... of proline for which the distance is smaller than the above. The uniform distribution of the C -s along the protein curve makes plots of the protein backbone relatively easy to do. The simplest solution (that we used already) is to connect the coordinates by a straight line interpolating from one C ...
... of proline for which the distance is smaller than the above. The uniform distribution of the C -s along the protein curve makes plots of the protein backbone relatively easy to do. The simplest solution (that we used already) is to connect the coordinates by a straight line interpolating from one C ...
Supplementary Data
... (hMRP3: gi:6920069), which provide intermediate links. The alanine content of the region aligned with the N-ter helix is in good agreement with such a secondary structure. Conserved hydrophobic amino acids (V, I, L, F, M, Y, W) are colored dark green, and residues that can substitute them (A, C, T) ...
... (hMRP3: gi:6920069), which provide intermediate links. The alanine content of the region aligned with the N-ter helix is in good agreement with such a secondary structure. Conserved hydrophobic amino acids (V, I, L, F, M, Y, W) are colored dark green, and residues that can substitute them (A, C, T) ...
The CS2 fimbrial antigen from escherichia coli, purification
... liberated by blending. Initial purification was obtained by isoelectric- and magnesium chloride precipitation and the protein was thereby isolated to approx. 80% purity in the form of intact fimbriae. Testing with ELISA showed that this preparation contained a small amount of material which gave a p ...
... liberated by blending. Initial purification was obtained by isoelectric- and magnesium chloride precipitation and the protein was thereby isolated to approx. 80% purity in the form of intact fimbriae. Testing with ELISA showed that this preparation contained a small amount of material which gave a p ...
Supplemental file S8 to
... Previously, major differences were identified among protein domains with regard to their presence in the genome and their protein domain recombination promiscuity [3,4]. It was found that some domains are always high ranking (e.g., SH3 and PH domains), whereas others are only present in relatively l ...
... Previously, major differences were identified among protein domains with regard to their presence in the genome and their protein domain recombination promiscuity [3,4]. It was found that some domains are always high ranking (e.g., SH3 and PH domains), whereas others are only present in relatively l ...
Scoring the Alignment of Amino Acid Sequences
... • The 1 PAM matrix was derived by constructing hypothetical phylogenetic trees relating sequences in 71 families. • The higher the power of the matrix, the more evolutionary time units represented by the matrix. • Criticism – raising M to high powers does not capture the true difference between shor ...
... • The 1 PAM matrix was derived by constructing hypothetical phylogenetic trees relating sequences in 71 families. • The higher the power of the matrix, the more evolutionary time units represented by the matrix. • Criticism – raising M to high powers does not capture the true difference between shor ...
Post-translational Modifications
... B. GPI anchors - Glycophosphatidyl inositol (GPI) attached to the C terminus Composed of oligosaccharides and inositol phospholipids Provides a mechanism for anchoring cell-surface proteins to the membrane as a flexible leash that allows the entire protein (except for anchor) to be in extracellula ...
... B. GPI anchors - Glycophosphatidyl inositol (GPI) attached to the C terminus Composed of oligosaccharides and inositol phospholipids Provides a mechanism for anchoring cell-surface proteins to the membrane as a flexible leash that allows the entire protein (except for anchor) to be in extracellula ...
Regulation of transcript encoding the 43K
... with negative numbers. The number of the nucleotide residue at the end of each line is provided. The predicted amino acid sequence is shown above the nucleotide sequence. Amino acid residues are numbered beginning with the amino terminal residue. The 5' and 3' ends of the Xen 43.1 cDNA is bordered b ...
... with negative numbers. The number of the nucleotide residue at the end of each line is provided. The predicted amino acid sequence is shown above the nucleotide sequence. Amino acid residues are numbered beginning with the amino terminal residue. The 5' and 3' ends of the Xen 43.1 cDNA is bordered b ...
Proteomic sensitivity to dietary manipulations in rainbow trout
... were found in free amino acid pools in either liver or muscle between diets. Protein extraction followed by high-resolution two-dimensional electrophoresis, coupled with gel image analysis, allowed identification and expression of hundreds of protein. Individual proteins of interest were then subjec ...
... were found in free amino acid pools in either liver or muscle between diets. Protein extraction followed by high-resolution two-dimensional electrophoresis, coupled with gel image analysis, allowed identification and expression of hundreds of protein. Individual proteins of interest were then subjec ...
Origin and Evolution of a New Gene Descended From alcohol
... D. mercatorum, though it does seem unlikely that the large deletions reported to exist in the protein-coding et al. (1994) could result regions amplified by SULLIVAN ...
... D. mercatorum, though it does seem unlikely that the large deletions reported to exist in the protein-coding et al. (1994) could result regions amplified by SULLIVAN ...
BIOLOGY
... and on their distances from each other. The signals that NMR produces are a set of distances between specific pairs of atoms. NMR data generate models of possible structures, rather than a single structure. For smaller proteins in particular, NMR can quite accurately predict the 3D structure. Despit ...
... and on their distances from each other. The signals that NMR produces are a set of distances between specific pairs of atoms. NMR data generate models of possible structures, rather than a single structure. For smaller proteins in particular, NMR can quite accurately predict the 3D structure. Despit ...
Allantoin transport protein, PucI, from Bacillus subtilis
... primates), however, is non-functional, so uric acid is the end product of purine catabolism and this is excreted in the urine (Johnson et al., 2009). In most other mammals, allantoin is excreted in the urine, whilst in fish it is degraded further to ammonia before excretion. In bacteria, purines are ...
... primates), however, is non-functional, so uric acid is the end product of purine catabolism and this is excreted in the urine (Johnson et al., 2009). In most other mammals, allantoin is excreted in the urine, whilst in fish it is degraded further to ammonia before excretion. In bacteria, purines are ...
Document
... lactating sows. However, metabolic traits such as plasma urea nitrogen [11] and the indicator AA oxidation technique [12] have also been used. For practical purposes, one would favor a response criterion such as daily gain. However, accurate estimates of daily gain can only be obtained when the expe ...
... lactating sows. However, metabolic traits such as plasma urea nitrogen [11] and the indicator AA oxidation technique [12] have also been used. For practical purposes, one would favor a response criterion such as daily gain. However, accurate estimates of daily gain can only be obtained when the expe ...
Circadian Rhythm of Intestinal SucraseActivity in Rats
... ceases. This rhythm can be temporally shifted by altering the time of controlled feeding (5), but can be eliminated only by several days of starvation (2). Because most investigators have found a rise in enzyme activity 1 h before the scheduled beginning of feeding, many have postulated that it is t ...
... ceases. This rhythm can be temporally shifted by altering the time of controlled feeding (5), but can be eliminated only by several days of starvation (2). Because most investigators have found a rise in enzyme activity 1 h before the scheduled beginning of feeding, many have postulated that it is t ...
to the reprint.
... comprised of four alpha helical segments. The second and third helices form a helix-turn-helix motif involved directly in DNA binding. The first and the fourth helixes are believed to contribute to the specificity of DNA-binding (5, 6) In the last decade, several families of homeobox genes have been ...
... comprised of four alpha helical segments. The second and third helices form a helix-turn-helix motif involved directly in DNA binding. The first and the fourth helixes are believed to contribute to the specificity of DNA-binding (5, 6) In the last decade, several families of homeobox genes have been ...
Integrative Assignment - California State University
... We already know how many amino acids are in your sequences from the GenPept pages. To get the proportion that is hydrophobic you can simply use the chart on page 47 of your book, tally the hydrophobic ones, and divide by the total number of amino acids in the protein. Should take about 10 minutes. T ...
... We already know how many amino acids are in your sequences from the GenPept pages. To get the proportion that is hydrophobic you can simply use the chart on page 47 of your book, tally the hydrophobic ones, and divide by the total number of amino acids in the protein. Should take about 10 minutes. T ...
Dr Asmat Salim MM707-electrophoresis 2014
... mainly by the buffer ions with a small proportion being conducted by the sample ions. • It is possible to accelerate an electrophoretic separation by increasing the applied voltage, which would result in a corresponding increase in the current flow. • The distance migrated will be proportional to cu ...
... mainly by the buffer ions with a small proportion being conducted by the sample ions. • It is possible to accelerate an electrophoretic separation by increasing the applied voltage, which would result in a corresponding increase in the current flow. • The distance migrated will be proportional to cu ...
enriched protein products of marine origin like the new components
... These properties make it possible to introduce protein ingredients in formulas of high-protein foods and obtain end products that are not inferior to traditional products in terms of the biological value and most importantly to efficiently use expensive raw materials of animal origin [6]. Products d ...
... These properties make it possible to introduce protein ingredients in formulas of high-protein foods and obtain end products that are not inferior to traditional products in terms of the biological value and most importantly to efficiently use expensive raw materials of animal origin [6]. Products d ...
The potato NLR immune receptor R3a does not contain
... positives among computationally predicted NLR-integrated domains. The position of predicted integrated domains needs to be checked for overlap with canonical NLR domains. Other false positives may include the DUF3542 domain, which overlaps with the CC domain of several solanaceous NLR proteins and m ...
... positives among computationally predicted NLR-integrated domains. The position of predicted integrated domains needs to be checked for overlap with canonical NLR domains. Other false positives may include the DUF3542 domain, which overlaps with the CC domain of several solanaceous NLR proteins and m ...
Analyzing Effects of Naturally Occurring Missense Mutations
... SNPs (nsSNPs), which cause changes in protein sequence. The most dramatic change is induced by the nonsense mutations, which result in a premature stop codon and produce truncated, usually nonfunctional proteins [4]. Missense mutation, on the other hand, is a change of a single amino acid into anoth ...
... SNPs (nsSNPs), which cause changes in protein sequence. The most dramatic change is induced by the nonsense mutations, which result in a premature stop codon and produce truncated, usually nonfunctional proteins [4]. Missense mutation, on the other hand, is a change of a single amino acid into anoth ...
Two Perspectives on the Origin of the Standard Genetic Code
... and using the method of stepwise fragment condensation of the randomly generated 10-mers available in the library. Even in such cases, yields can be low depending upon the conditions, with the synthesized oligomers showing no similarity with known proteins. These results highlight the difficulty of ...
... and using the method of stepwise fragment condensation of the randomly generated 10-mers available in the library. Even in such cases, yields can be low depending upon the conditions, with the synthesized oligomers showing no similarity with known proteins. These results highlight the difficulty of ...
The pyruvate dehydrogenase complex of the chemol
... which lacks pyruvate decarboxylase, dihydrolipoyl transacetylase and dihydrolipoamide dehydrogenase activities and is unable to grow on minimal medium lacking acetate whereas PDH positive strains can (Guest et al., 1983). Cosmid 5.1, plasmids pTHI081 and pTHI0251 were transformed into E. coli JRG134 ...
... which lacks pyruvate decarboxylase, dihydrolipoyl transacetylase and dihydrolipoamide dehydrogenase activities and is unable to grow on minimal medium lacking acetate whereas PDH positive strains can (Guest et al., 1983). Cosmid 5.1, plasmids pTHI081 and pTHI0251 were transformed into E. coli JRG134 ...
Molecular Imprinting of Maltose Binding Protein
... and covered by a mica wafer. The imprinted polymers (MIPs) were prepared in the same way by adding MBP labeled with Cy3 (MBP-Cy3) (1 mg mL1 in Tris buffer) to the precursor solution to achieve a final concentration of 0.5 mg mL1. Polymerization was conducted at 35 °C for 3 h. After polymerization, t ...
... and covered by a mica wafer. The imprinted polymers (MIPs) were prepared in the same way by adding MBP labeled with Cy3 (MBP-Cy3) (1 mg mL1 in Tris buffer) to the precursor solution to achieve a final concentration of 0.5 mg mL1. Polymerization was conducted at 35 °C for 3 h. After polymerization, t ...
Protein sequence databases
... The concept of protein superfamilies, families and domains is one of the oldest in computational biology. Back in the 60s, when the first protein sequence database was published as printed version, Margaret Dayhoff defined the basic principles of this discipline with only a small number of sequences ...
... The concept of protein superfamilies, families and domains is one of the oldest in computational biology. Back in the 60s, when the first protein sequence database was published as printed version, Margaret Dayhoff defined the basic principles of this discipline with only a small number of sequences ...