A Contribution of the Mitochondrial
... 1983; Timasheff, 1992; Sola-Penna and Meyer-Fernandes, 1994, 1996) have described the role of w ater in the maintenance of noncovalent interac tions in the enzymes. It has been reported that sol uble mitochondrial F, -ATPase (Garza-Ramos et al., 1989) and MgATP-submitochondrial particles (Garza-Ra ...
... 1983; Timasheff, 1992; Sola-Penna and Meyer-Fernandes, 1994, 1996) have described the role of w ater in the maintenance of noncovalent interac tions in the enzymes. It has been reported that sol uble mitochondrial F, -ATPase (Garza-Ramos et al., 1989) and MgATP-submitochondrial particles (Garza-Ra ...
Hydrogen exchange mass spectrometry for the analysis of protein
... FIGURE 1. Overall scheme for hydrogen exchange mass spectrometry experiments. A: Pulse labeling. After a protein has been exposed to a perturbant (chemical denaturant, heat, pH, binding, complex formation, pressure, etc.), unfolded regions (gray) become labeled with deuterium (red) during a quick pu ...
... FIGURE 1. Overall scheme for hydrogen exchange mass spectrometry experiments. A: Pulse labeling. After a protein has been exposed to a perturbant (chemical denaturant, heat, pH, binding, complex formation, pressure, etc.), unfolded regions (gray) become labeled with deuterium (red) during a quick pu ...
Python Libraries for Computational Chemistry and Biology
... Nowadays, everyone with $50,000 or more to spare is switching to an Oracle cartridge and using SQL. Still vendor specific, but much easier to use. Don’t need a special-purpose API - DB-API is just fine. ...
... Nowadays, everyone with $50,000 or more to spare is switching to an Oracle cartridge and using SQL. Still vendor specific, but much easier to use. Don’t need a special-purpose API - DB-API is just fine. ...
Bosque, a software system for phylogenetic analysis
... that will produce, ultimately, a phylogenetic tree. Thinking on this basic pipeline, Bosque defines the concept of Tree Project as a set consisting of: 1. a set of sequences 2. an alignment of these sequences, and 3. a set of trees out of this alignment Since there is no perfect tree-reconstruction ...
... that will produce, ultimately, a phylogenetic tree. Thinking on this basic pipeline, Bosque defines the concept of Tree Project as a set consisting of: 1. a set of sequences 2. an alignment of these sequences, and 3. a set of trees out of this alignment Since there is no perfect tree-reconstruction ...
The Calcium-Binding Activity of a Vacuole
... A vacuole membrane-associated calcium-binding protein with an apparent mass of 45 kD was purified from celery (Apium graveolens). This protein, VCaB45, is enriched in highly vacuolate tissues and is located within the lumen of vacuoles. Antigenically related proteins are present in many dicotyledono ...
... A vacuole membrane-associated calcium-binding protein with an apparent mass of 45 kD was purified from celery (Apium graveolens). This protein, VCaB45, is enriched in highly vacuolate tissues and is located within the lumen of vacuoles. Antigenically related proteins are present in many dicotyledono ...
A Simple Introduction to NCBI BLAST
... sequence (Figure 6a). Looking at the descriptions, it appears that our unknown D. yakuba genomic DNA has sequence homology to the gene legless in D. melanogaster. Comparing the descriptions for the top eight hits, we noticed that only the D. melanogaster hit has an accession number that begins with ...
... sequence (Figure 6a). Looking at the descriptions, it appears that our unknown D. yakuba genomic DNA has sequence homology to the gene legless in D. melanogaster. Comparing the descriptions for the top eight hits, we noticed that only the D. melanogaster hit has an accession number that begins with ...
Materials and Methods
... expression and solubility score values. A parameter can potentially show an equivalent effect across the entire score range; in this case, an increase in the parameter would have the same influence on the probability of scoring 0 vs. 1 for expression as for scoring 3 vs. 4 for expression. Alternatel ...
... expression and solubility score values. A parameter can potentially show an equivalent effect across the entire score range; in this case, an increase in the parameter would have the same influence on the probability of scoring 0 vs. 1 for expression as for scoring 3 vs. 4 for expression. Alternatel ...
A Major Surface Protein on Group A Streptococci Is a
... Dot-Blot Immunoassay to Determine the Location of the 39-kD Protein. The surface location of 39-kD protein was determined with the monospecific antibodies using a bacterial dot-blot immunoassay as previously described (31, 40). Essentially, an overnight culture of strain D471 was adjusted to ODium 1 ...
... Dot-Blot Immunoassay to Determine the Location of the 39-kD Protein. The surface location of 39-kD protein was determined with the monospecific antibodies using a bacterial dot-blot immunoassay as previously described (31, 40). Essentially, an overnight culture of strain D471 was adjusted to ODium 1 ...
ppt
... stages, a preliminary study using a small dataset to refine the system, followed by a benchmarking study using a larger dataset. The preliminary study used 146 abstracts, consisting of 56 positive papers and 90 negative papers: ...
... stages, a preliminary study using a small dataset to refine the system, followed by a benchmarking study using a larger dataset. The preliminary study used 146 abstracts, consisting of 56 positive papers and 90 negative papers: ...
X(0,25) - IFSC-USP
... Gly residues that turn in a way that only they can.These Glys are retained in all members of a fold family(ie. all Bacterial ribosomal proteins). Pro has a constrained conformation. While Pros can be replaced by other amino acids, specific energetic stability may be lost accounting for conservation. ...
... Gly residues that turn in a way that only they can.These Glys are retained in all members of a fold family(ie. all Bacterial ribosomal proteins). Pro has a constrained conformation. While Pros can be replaced by other amino acids, specific energetic stability may be lost accounting for conservation. ...
Health relevance of intestinal protein fermentation in young
... play only a minor role with regard to total pre-caecal protein digestibility (Erickson and Kim, 1990). As indicated below, there are some indications that dietary (i.e. digesta viscosity, dietary particle size, buffering capacity, antinutritional factors, diet changes) as well as endogenous factors ...
... play only a minor role with regard to total pre-caecal protein digestibility (Erickson and Kim, 1990). As indicated below, there are some indications that dietary (i.e. digesta viscosity, dietary particle size, buffering capacity, antinutritional factors, diet changes) as well as endogenous factors ...
STING Millennium: a web-based suite of programs
... because any point on the structure/surface might be painted (colored) only once, following the color code designed for given amino acid characteristics. Amino acid sequence on the other hand, can be mapped by number of parameters, organized and displayed in underlying rows. Appropriate visualization ...
... because any point on the structure/surface might be painted (colored) only once, following the color code designed for given amino acid characteristics. Amino acid sequence on the other hand, can be mapped by number of parameters, organized and displayed in underlying rows. Appropriate visualization ...
Exact and pattern searching of protein sequences
... Complex pattern searching of protein sequences is possible in the /SQSP and /SQSFP subsequence search fields by using the Boolean operators (AND, OR, NOT) as well as special characters and symbols. ...
... Complex pattern searching of protein sequences is possible in the /SQSP and /SQSFP subsequence search fields by using the Boolean operators (AND, OR, NOT) as well as special characters and symbols. ...
Distribution of major serum proteins in an airbrea
... saturation suggests resembling state of surface hydrophilic amino acid residues, which determines solubility under changed ionic strengths or the pH. Obviously, another β globulin, Hp undergoes such a change in state of solubility at 45% AS and may be exploited as first purification step. Low salt s ...
... saturation suggests resembling state of surface hydrophilic amino acid residues, which determines solubility under changed ionic strengths or the pH. Obviously, another β globulin, Hp undergoes such a change in state of solubility at 45% AS and may be exploited as first purification step. Low salt s ...
Survey - University of Washington
... Summary of BLAST • Dynamic programming is O(nm), where n is the length of the query and m is the size of the database. • BLAST is O(m). • BLAST produces an index of the query sequence that allows fast matching to the database. • Relative to Smith-Waterman, BLAST can produce false negatives; i.e., h ...
... Summary of BLAST • Dynamic programming is O(nm), where n is the length of the query and m is the size of the database. • BLAST is O(m). • BLAST produces an index of the query sequence that allows fast matching to the database. • Relative to Smith-Waterman, BLAST can produce false negatives; i.e., h ...
Crystal Structure of 4-Chlorobenzoate:CoA Ligase/Synthetase in the
... acids or adenylated benzoic acid derivatives. The nonribosomal peptide synthetases (NRPSs) are composed of numerous catalytic domains linked in a modular fashion, often within a single polypeptide that can be thousands of residues in length (9, 13, 14). The adenylation domains of the NRPSs serve as ...
... acids or adenylated benzoic acid derivatives. The nonribosomal peptide synthetases (NRPSs) are composed of numerous catalytic domains linked in a modular fashion, often within a single polypeptide that can be thousands of residues in length (9, 13, 14). The adenylation domains of the NRPSs serve as ...
Nutrient Metabolism
... ABSTRACT A restricted protein diet supplemented with amino acids and fat may reduce the acidogenic effects of exercise. Twelve Arabian horses were assigned to a 2 ⫻ 2 factorial experiment: two fat levels: 0 or 10 g/100 g added corn oil and two crude protein levels: 7.5 g/100 g (supplemented with 0.5 ...
... ABSTRACT A restricted protein diet supplemented with amino acids and fat may reduce the acidogenic effects of exercise. Twelve Arabian horses were assigned to a 2 ⫻ 2 factorial experiment: two fat levels: 0 or 10 g/100 g added corn oil and two crude protein levels: 7.5 g/100 g (supplemented with 0.5 ...
Protein design TOPS cartoon of Top7 Approach to designing Top7
... multimolecular assemblies by multiple docking’’. J. Mol. Biol., 349, 435-447] ...
... multimolecular assemblies by multiple docking’’. J. Mol. Biol., 349, 435-447] ...
Chapter Five - DORAS
... compared to ampicillin. Cultures of 50 ml were inoculated with 1 ml of a stationary phase overnight culture. For each strain, an induced and uninduced culture was prepared. The cultures were incubated at 37oC and 220 rpm until an OD680nm of 0.4 was reached. At this point IPTG was added to a concentr ...
... compared to ampicillin. Cultures of 50 ml were inoculated with 1 ml of a stationary phase overnight culture. For each strain, an induced and uninduced culture was prepared. The cultures were incubated at 37oC and 220 rpm until an OD680nm of 0.4 was reached. At this point IPTG was added to a concentr ...
Computer-Based Design of Novel Protein Structures
... amino acid sequences that could pack well on a target protein backbone. These algorithms have been successful and have been used to stabilize proteins, solubilize membrane proteins, redesign protein-protein interactions, create new enzymes, and design novel protein structures (6, 15, 35, 50, 72). In ...
... amino acid sequences that could pack well on a target protein backbone. These algorithms have been successful and have been used to stabilize proteins, solubilize membrane proteins, redesign protein-protein interactions, create new enzymes, and design novel protein structures (6, 15, 35, 50, 72). In ...
PCR primers for the amplification of the nuclear small subunit
... twice in filtered seawater and the central capsule was physically separated by a sterilized razor blade from the ectocytoplasm, which contained endosymbiotic algae, as mentioned above. Each central capsule and the ectocytoplasm was rinsed twice again in distilled water, and then incubated for 30 min ...
... twice in filtered seawater and the central capsule was physically separated by a sterilized razor blade from the ectocytoplasm, which contained endosymbiotic algae, as mentioned above. Each central capsule and the ectocytoplasm was rinsed twice again in distilled water, and then incubated for 30 min ...
Investigation of a Zα-like Peptide Motif in Koi Herpesvirus
... mutants using identical concentrations and the same procedure. In Figure 4, the conformational change in B-DNA with titration of ORF112 Y84A can be seen. Initial addition of protein causes an immediate inversion of CD bands from 250-265 nm, whereas in the wild type, inversion did not occur until tit ...
... mutants using identical concentrations and the same procedure. In Figure 4, the conformational change in B-DNA with titration of ORF112 Y84A can be seen. Initial addition of protein causes an immediate inversion of CD bands from 250-265 nm, whereas in the wild type, inversion did not occur until tit ...
