This is the accepted version of the following article:
... specific providing high yields and purity without the risk of structural or functional modification of isolated protein. Purifications based on affinity chromatography represent the most powerful tool available in term of the selectivity and recovery. Batch separation with specific antibodies as af ...
... specific providing high yields and purity without the risk of structural or functional modification of isolated protein. Purifications based on affinity chromatography represent the most powerful tool available in term of the selectivity and recovery. Batch separation with specific antibodies as af ...
Amino Acids, Peptides, and Proteins
... that ionize near pH 8): the amino-terminal a-amino group of Glu and the His imidazole group. Thus, we can estimate ...
... that ionize near pH 8): the amino-terminal a-amino group of Glu and the His imidazole group. Thus, we can estimate ...
Article Antibody-Specific Model of Amino Acid
... process constitute the first building block required for the development of accurate and efficient computational methodology capable to predict antibody properties from sequence data. As somatic hypermutation involves fast mutation of antibody sequences, this process can be described using a Markov ...
... process constitute the first building block required for the development of accurate and efficient computational methodology capable to predict antibody properties from sequence data. As somatic hypermutation involves fast mutation of antibody sequences, this process can be described using a Markov ...
Full Text
... The superfamily of ligand-gated ion channels (LGICs) has been implicated in anesthetic and alcohol responses. Mutations within glycine and GABA receptors have demonstrated that possible sites of anesthetic action exist within the transmembrane subunits of these receptors. The exact molecular arrange ...
... The superfamily of ligand-gated ion channels (LGICs) has been implicated in anesthetic and alcohol responses. Mutations within glycine and GABA receptors have demonstrated that possible sites of anesthetic action exist within the transmembrane subunits of these receptors. The exact molecular arrange ...
Identification of four small molecular mass proteins in the silk of
... genes have also been elucidated in the waxmoth, Galleria mellonella (suprafamily Pyraloidea), a lepidopteran distantly related to B. mori. It was found that the overall silk composition and the silk gene structure are similar as in B. mori (0urovec et al., 1992). Amino acid sequence homologies betwe ...
... genes have also been elucidated in the waxmoth, Galleria mellonella (suprafamily Pyraloidea), a lepidopteran distantly related to B. mori. It was found that the overall silk composition and the silk gene structure are similar as in B. mori (0urovec et al., 1992). Amino acid sequence homologies betwe ...
evolution of protein function by domain swapping
... high in function. Thus, an understanding of how proteins evolve is an important step in rationally designing combinatorial protein engineering strategies. It is generally accepted that nature evolves proteins for novel function by redesigning existing protein frameworks. This is supported by a numbe ...
... high in function. Thus, an understanding of how proteins evolve is an important step in rationally designing combinatorial protein engineering strategies. It is generally accepted that nature evolves proteins for novel function by redesigning existing protein frameworks. This is supported by a numbe ...
IndelFR: a database of indels in protein structures and their flanking
... D512–D518 Nucleic Acids Research, 2012, Vol. 40, Database issue ...
... D512–D518 Nucleic Acids Research, 2012, Vol. 40, Database issue ...
BCH364C-391L-Spring2015-SequenceAlignmentI
... In practice, searching for sequence or structural similarity is one of the most powerful computational approaches for discovering functions for genes, since we can often glean many new insights about a protein based on what is known about its homologs. Here’s an example from my own lab, where we di ...
... In practice, searching for sequence or structural similarity is one of the most powerful computational approaches for discovering functions for genes, since we can often glean many new insights about a protein based on what is known about its homologs. Here’s an example from my own lab, where we di ...
Importance of main-chain hydrophobic free energy to the stability of
... Several investigations have been carried out to understand the mechanism for the thermostability of proteins. Gromiha et al. [17] made a comparative analysis on the relation between thermostability and amino acid properties for a family of meso and thermophilic proteins wherein the Gibbs free energy ...
... Several investigations have been carried out to understand the mechanism for the thermostability of proteins. Gromiha et al. [17] made a comparative analysis on the relation between thermostability and amino acid properties for a family of meso and thermophilic proteins wherein the Gibbs free energy ...
PDF only - at www.arxiv.org.
... One of the main roles of bioinformatics (or computational biology) in this post-genomic era of biology is to reduce the workload of the experimentalists by computationally “eliminating” candidates for experimentation, thereby allowing them to invest their time and effort on the “good” ones that are ...
... One of the main roles of bioinformatics (or computational biology) in this post-genomic era of biology is to reduce the workload of the experimentalists by computationally “eliminating” candidates for experimentation, thereby allowing them to invest their time and effort on the “good” ones that are ...
The Roles of Amino Acids in Milk Yield and Components
... the rumen, of which microbial proteins supply the majority with variable contributions from undigested feed proteins. In the core essential group, the branched chain AAs, methionine and phenylalanine can be synthesized by animals from their corresponding keto-acids. However, the keto-acids can only ...
... the rumen, of which microbial proteins supply the majority with variable contributions from undigested feed proteins. In the core essential group, the branched chain AAs, methionine and phenylalanine can be synthesized by animals from their corresponding keto-acids. However, the keto-acids can only ...
Protein Applications in Sports Nutrition—Part I
... been consistently training for over a year (45,47). Phillips et al. (45) demonstrated in the same group of initially untrained participants that 8 weeks of resistance training (6 d/wk) blunted the acute MPS response seen at baseline while consuming .1.2 6 0.6 g$kg21$d21 of protein. Despite this att ...
... been consistently training for over a year (45,47). Phillips et al. (45) demonstrated in the same group of initially untrained participants that 8 weeks of resistance training (6 d/wk) blunted the acute MPS response seen at baseline while consuming .1.2 6 0.6 g$kg21$d21 of protein. Despite this att ...
Crystal structure of yeast hexokinase Pl in complex
... A multiple sequence alignment of hexokinases was obtained from the PFAM database,41 in a total of 317 sequences, including that of yeast hexokinase PI. From the alignment, it is possible to compute, in a parameter named DG stat, the discrepancy between amino acid frequencies in a given position and ...
... A multiple sequence alignment of hexokinases was obtained from the PFAM database,41 in a total of 317 sequences, including that of yeast hexokinase PI. From the alignment, it is possible to compute, in a parameter named DG stat, the discrepancy between amino acid frequencies in a given position and ...
Plant organelle proteomics
... There is a plethora of information about cellular mechanisms in plants that we glean by studying mutants or changes in transcript levels, especially in Arabidopsis thaliana. The study of genes however is only a limited dimension for cell biologists. The study of the proteome is far more information ...
... There is a plethora of information about cellular mechanisms in plants that we glean by studying mutants or changes in transcript levels, especially in Arabidopsis thaliana. The study of genes however is only a limited dimension for cell biologists. The study of the proteome is far more information ...
SURVEY AND SUMMARY Origins of tmRNA: the
... for these biochemical activities. This evolutionary leap was embodied by the emergence of non-random coding RNA, which served as the first medium for genetic information. From there, RNA and peptides had to co-evolve through a ribonucleoprotein (RNP) world (Figure 1). This step would have been funda ...
... for these biochemical activities. This evolutionary leap was embodied by the emergence of non-random coding RNA, which served as the first medium for genetic information. From there, RNA and peptides had to co-evolve through a ribonucleoprotein (RNP) world (Figure 1). This step would have been funda ...
Structure and biosynthesis of the signal
... is synthesized with a cleavable amino-terminal signal sequence and contains only one classical membrane-spanning segment. Its insertion into the ER membrane during biosynthesis depends on the function of the signal-recognition particle. SSR shows a remarkable charge distribution with the amino termi ...
... is synthesized with a cleavable amino-terminal signal sequence and contains only one classical membrane-spanning segment. Its insertion into the ER membrane during biosynthesis depends on the function of the signal-recognition particle. SSR shows a remarkable charge distribution with the amino termi ...
Relationships Analyzing Amino-Acid Sequences to Determine
... 1. According to your data from Cytochrome C, which organism is most closely related to humans? Which is least closely related to humans? 2. In the study of hemoglobin, which vertebrate is most closely related to humans? Least closely related? 3. Why can it be said that proteins behave like molecular ...
... 1. According to your data from Cytochrome C, which organism is most closely related to humans? Which is least closely related to humans? 2. In the study of hemoglobin, which vertebrate is most closely related to humans? Least closely related? 3. Why can it be said that proteins behave like molecular ...
Biological Science, 5e (Freeman) Chapter 3 Protein Structure and
... Since structure correlates well with function, look for new ways to probe the complex structure of proteins in order to understand what they do and how they do it. One of the most powerful techniques in existence today is X-ray crystallography. The main difficulty with this technique is getting the ...
... Since structure correlates well with function, look for new ways to probe the complex structure of proteins in order to understand what they do and how they do it. One of the most powerful techniques in existence today is X-ray crystallography. The main difficulty with this technique is getting the ...
Fragmenta Agronomica 2
... – year of the experiment. The content of isoleucine, on the other hand, was significantly lower. In the case of endogenous amino acids, in the second year of the study their total content was 59.39 g·100 g-1 of protein, and was significantly higher compared to the first year, by 3.15 g, i.e. by 5.6% ...
... – year of the experiment. The content of isoleucine, on the other hand, was significantly lower. In the case of endogenous amino acids, in the second year of the study their total content was 59.39 g·100 g-1 of protein, and was significantly higher compared to the first year, by 3.15 g, i.e. by 5.6% ...
Basis of preclinical studies_Biochemistry_Practicals_LI
... 4. Be able to discuss the significance of alterations of protein amount in blood serum. 1) Biuret reaction Principle: The biuret reaction is a method that can be used to determine the amount of soluble protein in a solution. The biuret reagent (copper sulfate in a strong base) reacts with peptide bo ...
... 4. Be able to discuss the significance of alterations of protein amount in blood serum. 1) Biuret reaction Principle: The biuret reaction is a method that can be used to determine the amount of soluble protein in a solution. The biuret reagent (copper sulfate in a strong base) reacts with peptide bo ...
3.2 Breeding of corn line DAS-40278-9
... studies with the AAD-1 protein have confirmed the absence of any biologically significant amino acid sequence similarity to known protein toxins or allergens and digestibility studies have demonstrated that the protein would be rapidly degraded following ingestion, similar to other dietary proteins. ...
... studies with the AAD-1 protein have confirmed the absence of any biologically significant amino acid sequence similarity to known protein toxins or allergens and digestibility studies have demonstrated that the protein would be rapidly degraded following ingestion, similar to other dietary proteins. ...
The novel genome organization of the insect picorna
... that the proteins may be translated through internal initiation of translation from the genomic length RNA. The finding of this novel genome organization for DCV shows that this virus is not a member of the Picornaviridae as previously thought, but belongs to a distinct and hitherto unrecognized vir ...
... that the proteins may be translated through internal initiation of translation from the genomic length RNA. The finding of this novel genome organization for DCV shows that this virus is not a member of the Picornaviridae as previously thought, but belongs to a distinct and hitherto unrecognized vir ...
Stabilization by GroEL, a Molecular Chaperone, and a Periplasmic
... only a few such proteins are known. Furthermore, the periplasm of Escherichia coli has been of great interest with respect to the functional expression of a wide variety of recombinant proteins from different sources. However, the periplasmic folding of proteins has not been studied in any great det ...
... only a few such proteins are known. Furthermore, the periplasm of Escherichia coli has been of great interest with respect to the functional expression of a wide variety of recombinant proteins from different sources. However, the periplasmic folding of proteins has not been studied in any great det ...