Gene Section DLX6 (distal-less homeobox 6) Atlas of Genetics and Cytogenetics
... between defective DLX5/DLX6 expression and the pathogenic mechanism impairing limb development remains to be elucidated. In the second case, the mutated DLX5 has been demonstrated to fail at transactivating its bona fide MYC target. Such an observation is not unexpected as the mutation affects Q50, ...
... between defective DLX5/DLX6 expression and the pathogenic mechanism impairing limb development remains to be elucidated. In the second case, the mutated DLX5 has been demonstrated to fail at transactivating its bona fide MYC target. Such an observation is not unexpected as the mutation affects Q50, ...
Engineering carbonic anhydrase for highly selective ester hydrolysis Gunnar Höst
... tuning of protein parameters, without being critical for the structural integrity. By introducing variation in such positions the catalytic performance of an enzyme can be changed without destroying its ability to fold. Prior to the advent of recombinant technology and protein engineering, the only ...
... tuning of protein parameters, without being critical for the structural integrity. By introducing variation in such positions the catalytic performance of an enzyme can be changed without destroying its ability to fold. Prior to the advent of recombinant technology and protein engineering, the only ...
Plasmodium falciparum enolase - Tata Institute of Fundamental
... (Pfen) (EC 4.2.1.11), the dehydrating glycolytic metalloenzyme that catalyzes the inter conversion of 2-phosphoglyceric acid (2-PGA) and phosphoenolpyruvate (PEP), has not yet been characterized. Enolases are highly conserved across species [9]. In most species, it exists as a symmetric homodimer [1 ...
... (Pfen) (EC 4.2.1.11), the dehydrating glycolytic metalloenzyme that catalyzes the inter conversion of 2-phosphoglyceric acid (2-PGA) and phosphoenolpyruvate (PEP), has not yet been characterized. Enolases are highly conserved across species [9]. In most species, it exists as a symmetric homodimer [1 ...
Identification of Novel Non-Metal Haloperoxidases from the Marine
... dispensed in 96-deep-square well plates, and the plates were incubated overnight at 37°C using a plate orbital shaker (Titramax 1000; Heidolph). Cultures were harvested by centrifugation (2,500 rpm for 10 min) and frozen at -80°C for 60 min. For the HPO assay, cells were suspended in 1,000 µl of 0.5 ...
... dispensed in 96-deep-square well plates, and the plates were incubated overnight at 37°C using a plate orbital shaker (Titramax 1000; Heidolph). Cultures were harvested by centrifugation (2,500 rpm for 10 min) and frozen at -80°C for 60 min. For the HPO assay, cells were suspended in 1,000 µl of 0.5 ...
The photosynthetic apparatus of Prochlorococcus
... light-harvesting antenna systems of a single genus, Prochlorococcus. This organism, discovered only about ten years ago, is an extremely small, Chl b-containing cyanobacterium that sometimes constitutes up to 50 % of the photosynthetic biomass in the oceans. Various Prochlorococcus strains are known ...
... light-harvesting antenna systems of a single genus, Prochlorococcus. This organism, discovered only about ten years ago, is an extremely small, Chl b-containing cyanobacterium that sometimes constitutes up to 50 % of the photosynthetic biomass in the oceans. Various Prochlorococcus strains are known ...
Nucleic Acids Research, 32: D489-D492 (2004).
... suggest that Alu elements have a broad evolutionary impact. Parts of Alu elements may become inserted into mature mRNAs by way of splicing in a process called `exonization'. Presumably, the exonization process is facilitated by sequence motifs within Alu that resemble splice sites (4±6). Indeed, mor ...
... suggest that Alu elements have a broad evolutionary impact. Parts of Alu elements may become inserted into mature mRNAs by way of splicing in a process called `exonization'. Presumably, the exonization process is facilitated by sequence motifs within Alu that resemble splice sites (4±6). Indeed, mor ...
Ectopic overexpression of castor bean LEAFY
... plants identified orthologs of master regulators of seed filling originally identified in Arabidopsis, such as LEC2, ABI3, and FUS3 [29,30]. Sequence-based quantitative trait loci (QTL) analysis has associated many rice and maize genes that encode products containing B3 domain genes with traits such as ...
... plants identified orthologs of master regulators of seed filling originally identified in Arabidopsis, such as LEC2, ABI3, and FUS3 [29,30]. Sequence-based quantitative trait loci (QTL) analysis has associated many rice and maize genes that encode products containing B3 domain genes with traits such as ...
Computational Definition of
... We have used a computational approach to identify ESEs and ESSs associated with constitutively spliced exons. We focused on constitutive splicing as opposed to alternative splicing so as to tackle the more fundamental problem represented by the former and to avoid what are likely more complex mechan ...
... We have used a computational approach to identify ESEs and ESSs associated with constitutively spliced exons. We focused on constitutive splicing as opposed to alternative splicing so as to tackle the more fundamental problem represented by the former and to avoid what are likely more complex mechan ...
CP1 Domain in Escherichia coli Leucyl
... steps; amino acids larger than the cognate substrate are rejected by a “coarse sieve”, while the reaction products of amino acids smaller than the cognate substrate will go through a “fine sieve” and be hydrolyzed. This “double-sieve” mechanism has been proposed for IleRS, a class I aminoacyl-tRNA s ...
... steps; amino acids larger than the cognate substrate are rejected by a “coarse sieve”, while the reaction products of amino acids smaller than the cognate substrate will go through a “fine sieve” and be hydrolyzed. This “double-sieve” mechanism has been proposed for IleRS, a class I aminoacyl-tRNA s ...
RHD - Labex
... The results with Cp beyond 40 cycles are not to be considered as definitive, they must be checked ...
... The results with Cp beyond 40 cycles are not to be considered as definitive, they must be checked ...
abnormalities of chromosome structure
... MUTAGENS: agents that are known to increase the frequency of mutations. Radiation from x-rays or nuclear fallout forms electrically charged ions that produce chemical reactions which change DNA bases. A variety of chemicals can induce mutations because they are chemically similar to the DNA ba ...
... MUTAGENS: agents that are known to increase the frequency of mutations. Radiation from x-rays or nuclear fallout forms electrically charged ions that produce chemical reactions which change DNA bases. A variety of chemicals can induce mutations because they are chemically similar to the DNA ba ...
Characterisation of the katA gene encoding a catalase and evidence
... The catalase gene katA of Staphylococcus xylosus was cloned. It encodes a protein of 494 amino acids with a molecular mass of 56.9 kDa, closely related to monofunctional catalases. A katA mutant still showed a relatively high catalase activity demonstrating that S. xylosus possesses more than one en ...
... The catalase gene katA of Staphylococcus xylosus was cloned. It encodes a protein of 494 amino acids with a molecular mass of 56.9 kDa, closely related to monofunctional catalases. A katA mutant still showed a relatively high catalase activity demonstrating that S. xylosus possesses more than one en ...
Chemical synthesis of proteins
... for specific functions. There are three general chemical approaches to constructing proteins. The first is ‘stepwise synthesis’, in which the entire protein is synthesized one amino acid at a time. The second is ‘fragment assembly’, in which individual peptide strands are initially constructed stepw ...
... for specific functions. There are three general chemical approaches to constructing proteins. The first is ‘stepwise synthesis’, in which the entire protein is synthesized one amino acid at a time. The second is ‘fragment assembly’, in which individual peptide strands are initially constructed stepw ...
Pancreas
... 1) fatty acid (18 C) + coenzyme A 2) fatty acid (18 C)-coA 3) fatty acid (16 C) and acetyl-coA Acetyl-CoA used in citric acid cycle This reaction also yields NADH => electron transport chain Excess acetyl-CoA forms ketone bodies ...
... 1) fatty acid (18 C) + coenzyme A 2) fatty acid (18 C)-coA 3) fatty acid (16 C) and acetyl-coA Acetyl-CoA used in citric acid cycle This reaction also yields NADH => electron transport chain Excess acetyl-CoA forms ketone bodies ...
The energetic basis of the DNA double helix: a
... rather controversial in a number of aspects. The first DSC studies of natural DNA suggested that their unfolding/dissociation proceeds without noticeable heat capacity increment, Cp (9). Since according to Kirchhoff’s relation, Cp = ∂(H)/∂T, it was assumed, and widely accepted, that the enthalpy ...
... rather controversial in a number of aspects. The first DSC studies of natural DNA suggested that their unfolding/dissociation proceeds without noticeable heat capacity increment, Cp (9). Since according to Kirchhoff’s relation, Cp = ∂(H)/∂T, it was assumed, and widely accepted, that the enthalpy ...
SNP-Based Mapping of Crossover Recombination in
... limited in most cases to two markers (due to the relative paucity of morphological phenotypes in C. elegans) . As a result, each experiment typically measures crossover frequency within a sin gle interval, which prevents detection of chromosomes with mul tiple crossovers and complicates determina ...
... limited in most cases to two markers (due to the relative paucity of morphological phenotypes in C. elegans) . As a result, each experiment typically measures crossover frequency within a sin gle interval, which prevents detection of chromosomes with mul tiple crossovers and complicates determina ...
Base Composition and Translational Selection are Insufficient to
... All organisms exhibit some degree of codon usage bias (CUB), the unequal usage of synonymous codons [1,2]. Codon bias may vary among genes of the same organism, which is associated with factors like asymmetrical mutation pressures or tissue-specific gene expression, but is relatively uniform within ...
... All organisms exhibit some degree of codon usage bias (CUB), the unequal usage of synonymous codons [1,2]. Codon bias may vary among genes of the same organism, which is associated with factors like asymmetrical mutation pressures or tissue-specific gene expression, but is relatively uniform within ...
Translational control of regA, a key gene controlling
... with which the control construct (pVcRegA1, shortend A1) restored the WT phenotype equal to 100%. Mutational ablation of AUG1, AUG2 or ORF2 causes a marked reduction of regA expression Here, we use RNA nomenclature (AUG, etc) when it concerns translation control, and DNA nomenclature (ATG, etc) when ...
... with which the control construct (pVcRegA1, shortend A1) restored the WT phenotype equal to 100%. Mutational ablation of AUG1, AUG2 or ORF2 causes a marked reduction of regA expression Here, we use RNA nomenclature (AUG, etc) when it concerns translation control, and DNA nomenclature (ATG, etc) when ...
Doctorial Thesis Regulation of Branched
... Leucine is an essential branched-chain amino acid and plays a key role in protein synthesis through mammalian target of rapamycin (mTOR). Recently, it has been reported that an oral administration of leucine has been revealed to influence the plasma concentrations of other amino acids not only isole ...
... Leucine is an essential branched-chain amino acid and plays a key role in protein synthesis through mammalian target of rapamycin (mTOR). Recently, it has been reported that an oral administration of leucine has been revealed to influence the plasma concentrations of other amino acids not only isole ...
Saccharomyces cerevisiae Functional Expression and Stabilization of Horseradish Peroxidase by Directed Evolution in
... Since total activity of wild-type HRP-C in S. cerevisiae is near the detection limit for ABTS as substrate, a much more highly expressed HRP mutant, 2-13A10 (Morawski et al., 2000) was used as the starting point for directed evolution by random mutagenesis. HRP 2-13A10 is less thermostable than wild ...
... Since total activity of wild-type HRP-C in S. cerevisiae is near the detection limit for ABTS as substrate, a much more highly expressed HRP mutant, 2-13A10 (Morawski et al., 2000) was used as the starting point for directed evolution by random mutagenesis. HRP 2-13A10 is less thermostable than wild ...
6 Energy and Metabolism
... of, but almost no one wants to carry that burden. • There is a guy named NAD who is willing to accept this burden. When he takes on the H+, he is reduced. If his H+ burden is removed by someone else, he feels good, and is oxidized! • All of NAD’s brothers are also named NAD. It takes 2 NAD brothers ...
... of, but almost no one wants to carry that burden. • There is a guy named NAD who is willing to accept this burden. When he takes on the H+, he is reduced. If his H+ burden is removed by someone else, he feels good, and is oxidized! • All of NAD’s brothers are also named NAD. It takes 2 NAD brothers ...
AMINO ACID SEQUENCE ANALYSIS OF HUMAN INTERLEUKIN 1
... messenger RNA coding for IL-1. Lomedico, et al. (12) have published a cloned cDNA coding for P388Dl-derived murine IL-1. This nucleotide sequence was validated by the sequencing of two peptides derived by CNBr cleavage of purified P388D1 IL-1. Nevertheless, the protein encoded by this cDNA had very ...
... messenger RNA coding for IL-1. Lomedico, et al. (12) have published a cloned cDNA coding for P388Dl-derived murine IL-1. This nucleotide sequence was validated by the sequencing of two peptides derived by CNBr cleavage of purified P388D1 IL-1. Nevertheless, the protein encoded by this cDNA had very ...
Translocation Arrest by Reversible Folding of a Precursor Protein
... made use of the following observation. Import and processing of a fusion protein between a mitochondrial presequence (amino acid residues 1-22 of cytochrome oxidase subunit IV) and dihydrofolate reductase (DHFR) t was found to be inhibited after binding of the folate antagonist methotrexate to the D ...
... made use of the following observation. Import and processing of a fusion protein between a mitochondrial presequence (amino acid residues 1-22 of cytochrome oxidase subunit IV) and dihydrofolate reductase (DHFR) t was found to be inhibited after binding of the folate antagonist methotrexate to the D ...
Point mutation
A point mutation, or single base modification, is a type of mutation that causes a single nucleotide base change, insertion, or deletion of the genetic material, DNA or RNA. The term frameshift mutation indicates the addition or deletion of a base pair. A point mutant is an individual that is affected by a point mutation.Repeat induced point mutations are recurring point mutations, discussed below.