results and discussion discussion
... incubated at 37 °C. After overnight incubation, some of the mutants were not showing growth on LB ampicillin/kanamycin containing 750 mM NaCl agar plates. This clearly indicates the disruption in the salt tolerance gene or regulatory region. Six salt sensitive insertional mutants were obtained for p ...
... incubated at 37 °C. After overnight incubation, some of the mutants were not showing growth on LB ampicillin/kanamycin containing 750 mM NaCl agar plates. This clearly indicates the disruption in the salt tolerance gene or regulatory region. Six salt sensitive insertional mutants were obtained for p ...
Defelipe, L.A, Dolghih E, Roitberg A.E., Nouzova M., Mayoral
... in a hydrophobic pocket formed by Ile-151, Ile-154, Tyr 155, Leu158, Val-221 and Val-224 (Fig. 4A). Docking results indicated a clear difference in the interaction of (10R)-JHA and (10S)-JHA with the hydrophobic pocket of the binding site of AeJHAMT. Specifically, while the epoxide ring of (10S)-JHA ...
... in a hydrophobic pocket formed by Ile-151, Ile-154, Tyr 155, Leu158, Val-221 and Val-224 (Fig. 4A). Docking results indicated a clear difference in the interaction of (10R)-JHA and (10S)-JHA with the hydrophobic pocket of the binding site of AeJHAMT. Specifically, while the epoxide ring of (10S)-JHA ...
Structure and function of human lactalbumin made lethal to tumor
... field has widened in scope, acquiring new members and enriching our understanding of the basic principles underlying protein self-assembly and acquisition of new functionality. HAMLET key features are related to the intrinsic properties of proteins to possess varying functions depending on their conf ...
... field has widened in scope, acquiring new members and enriching our understanding of the basic principles underlying protein self-assembly and acquisition of new functionality. HAMLET key features are related to the intrinsic properties of proteins to possess varying functions depending on their conf ...
Product Information Sheet - Sigma
... Storage/Stability In dried form, hirudin was found to lose <5% of its activity in 2-3 years, when stored at –20 °C. In water with preservative added, it is stable for 6 months at room temperature. It is also stable when heated for 15 minutes at 80 °C. The heat stability decreases with increasing pH. ...
... Storage/Stability In dried form, hirudin was found to lose <5% of its activity in 2-3 years, when stored at –20 °C. In water with preservative added, it is stable for 6 months at room temperature. It is also stable when heated for 15 minutes at 80 °C. The heat stability decreases with increasing pH. ...
Introduction to amino acid metabolism Overview - Rose
... In animals, addition of free ammonium to a-ketoacids or to a-amino acids is limited to a-ketoglutarate, glutamate, and glycine. All other amino acids receive their nitrogen by transfer of organic nitrogen from one amino acid to another. In amino acid metabolism, the most common nitrogen donor is glu ...
... In animals, addition of free ammonium to a-ketoacids or to a-amino acids is limited to a-ketoglutarate, glutamate, and glycine. All other amino acids receive their nitrogen by transfer of organic nitrogen from one amino acid to another. In amino acid metabolism, the most common nitrogen donor is glu ...
The Chemical Composition of the Cell Wall in some
... In most cases strains of known origin have been used, either from the NCTC or NCIB. These were checked for purity, but not investigated further. Strains of Corynebacterium diphtheriae had been isolated from cases, and had the typical morphology and fermentation reactions. Strains of C . hofmanni iso ...
... In most cases strains of known origin have been used, either from the NCTC or NCIB. These were checked for purity, but not investigated further. Strains of Corynebacterium diphtheriae had been isolated from cases, and had the typical morphology and fermentation reactions. Strains of C . hofmanni iso ...
Characterization of the Role of Eco1 in Chromosome
... Before DNA replication, each chromosome is a single DNA molecule, referred to separately as chromatids (Figure 2). During the Synthesis (S) phase, the genetic material is replicated, forming sister chromatids. After replication, each of the chromosomes has a duplicate copy, now existing as a comple ...
... Before DNA replication, each chromosome is a single DNA molecule, referred to separately as chromatids (Figure 2). During the Synthesis (S) phase, the genetic material is replicated, forming sister chromatids. After replication, each of the chromosomes has a duplicate copy, now existing as a comple ...
The Glutaric Acidurias of the Amish: A Sense of Progress 1988
... collagens, elastins, or fibrillins. Rik also cared for a collection of patients with neuromuscular problems including WerdnigHoffman Disease, congenital metabolic myopathies and cardiomyopathies. Two brothers with increased 3-methylglutaconic acid in the urine, cyclic neuropenia, and cardiomyopathy ...
... collagens, elastins, or fibrillins. Rik also cared for a collection of patients with neuromuscular problems including WerdnigHoffman Disease, congenital metabolic myopathies and cardiomyopathies. Two brothers with increased 3-methylglutaconic acid in the urine, cyclic neuropenia, and cardiomyopathy ...
The DNA sequence and biology of human chromosome 19
... Figure S1: Chromosome 11 recombination rate versus sequence-based physical distance. Markers from the deCODE genetic map were aligned to the chromosome and the average recombination rate was calculated for each 1 Mb window along its length. Female, male, and sex-averaged recombination rates are indi ...
... Figure S1: Chromosome 11 recombination rate versus sequence-based physical distance. Markers from the deCODE genetic map were aligned to the chromosome and the average recombination rate was calculated for each 1 Mb window along its length. Female, male, and sex-averaged recombination rates are indi ...
Growth medium composition-determined regulatory mechanisms
... Transcriptional activation from the pheBA and catBCA promoters is regulated by CatR and the catechol degradation pathway intermediate cis,cis-muconate. Here it is shown that physiological control mechanisms are superimposed on this regulatory system. Transcriptional activation from the pheBA and cat ...
... Transcriptional activation from the pheBA and catBCA promoters is regulated by CatR and the catechol degradation pathway intermediate cis,cis-muconate. Here it is shown that physiological control mechanisms are superimposed on this regulatory system. Transcriptional activation from the pheBA and cat ...
An Update on the Hereditary Spastic Paraplegias: New Genes and
... sequencing (NGS) has led to the accelerated discovery of new HSP genes, including B4GALNT1, DDHD1, C19orf12, GBA2, TECPR2, DDHD2, C12orf65, REEP2, and IBA57. Moreover, a single study alone identified 18 previously unknown putative HSP genes and created a model for the protein interactions of HSP, cal ...
... sequencing (NGS) has led to the accelerated discovery of new HSP genes, including B4GALNT1, DDHD1, C19orf12, GBA2, TECPR2, DDHD2, C12orf65, REEP2, and IBA57. Moreover, a single study alone identified 18 previously unknown putative HSP genes and created a model for the protein interactions of HSP, cal ...
Amino Acid Interrelationships in Cysteine Toxicity in
... The results in Tables 1 and 3 show that in the examined strains of Neurospora crassa, S-methyl-L-cysteinewas a powerful and competitive antagonist of L-cysteine. In view of the reported occurrence of S-methyl-L-cysteineas a normal metabolite in the wild and methionineless strains of N . crassa and t ...
... The results in Tables 1 and 3 show that in the examined strains of Neurospora crassa, S-methyl-L-cysteinewas a powerful and competitive antagonist of L-cysteine. In view of the reported occurrence of S-methyl-L-cysteineas a normal metabolite in the wild and methionineless strains of N . crassa and t ...
Nucleotide Catabolism
... and produce antibodies. Adenosine deaminase activity is also impaired in other diseases such as AIDS, anemia, lymphomas and leukemias. Adenosine deaminase deficiency is another target for gene therapy. Gene therapy is the attempt to repair a genetic deficiency by the introduction of a function gene. ...
... and produce antibodies. Adenosine deaminase activity is also impaired in other diseases such as AIDS, anemia, lymphomas and leukemias. Adenosine deaminase deficiency is another target for gene therapy. Gene therapy is the attempt to repair a genetic deficiency by the introduction of a function gene. ...
INDIGO-BINDING DOMAINS IN CELLULASE MOLECULES
... that a certain relationship exists between the washing performance of enzymes and (i) quantity (percentage) of aromatic amino acid side chains exposed to solvent on the surface of protein globules or (ii) overall percentage of the surface non-polar residues. Two enzymes (Eg3 and Eg5) had higher cont ...
... that a certain relationship exists between the washing performance of enzymes and (i) quantity (percentage) of aromatic amino acid side chains exposed to solvent on the surface of protein globules or (ii) overall percentage of the surface non-polar residues. Two enzymes (Eg3 and Eg5) had higher cont ...
Biochemistry of Cells
... when protein chains coil or fold When protein chains called polypeptides join together, the tertiary structure forms because R groups interact with each other ...
... when protein chains coil or fold When protein chains called polypeptides join together, the tertiary structure forms because R groups interact with each other ...
Acute Phase Proteins - a Potent Biomarker for Mastitis
... tissue can be a source of the APP in bovine milk (Molenaar et al., 2002; Hiss et al., 2004). The secretion of APP in milk during IMI is presumably related to their roles in innate immunity in resisting the invasion and establishment of pathogens. Haptoglobin acts in plasma as a scavenger molecule fo ...
... tissue can be a source of the APP in bovine milk (Molenaar et al., 2002; Hiss et al., 2004). The secretion of APP in milk during IMI is presumably related to their roles in innate immunity in resisting the invasion and establishment of pathogens. Haptoglobin acts in plasma as a scavenger molecule fo ...
Biochemistry of Cells - Campbell County Schools
... when protein chains coil or fold When protein chains called polypeptides join together, the tertiary structure forms because R groups interact with each other ...
... when protein chains coil or fold When protein chains called polypeptides join together, the tertiary structure forms because R groups interact with each other ...
UNIT 1: INTRODUCTION TO BIOLOGY
... are the pairs of identical shaped and sized chromosomes in the cells of more complex, diploid organisms they are the result of sexual reproduction after fusion of male and female haploid gametes, each having only one set of chromosomes, to form a new, diploid organism diploid organisms have tw ...
... are the pairs of identical shaped and sized chromosomes in the cells of more complex, diploid organisms they are the result of sexual reproduction after fusion of male and female haploid gametes, each having only one set of chromosomes, to form a new, diploid organism diploid organisms have tw ...
In(IL mat A
... opposite mating type are heterokaryon-incompatible in the vegetative phase. This hypothesis was supported by the finding that when H4250 duplications are homozygous mat A/mat A or mat a/mat.a, they do not show the DA phenotype. Also, when other duplications are heterozygous for heterokaryon incompat ...
... opposite mating type are heterokaryon-incompatible in the vegetative phase. This hypothesis was supported by the finding that when H4250 duplications are homozygous mat A/mat A or mat a/mat.a, they do not show the DA phenotype. Also, when other duplications are heterozygous for heterokaryon incompat ...
Chapter 1. introduction
... 2.2. Storage of Biological information in DNA Genome: The total genetic informational content for each cell Exact duplication Expression of stored information DNA molecules Watson and Crick in 1952: Double helix structure Complementary base pairs by specific hydrogen bondings: C-G and A-T C-G: trip ...
... 2.2. Storage of Biological information in DNA Genome: The total genetic informational content for each cell Exact duplication Expression of stored information DNA molecules Watson and Crick in 1952: Double helix structure Complementary base pairs by specific hydrogen bondings: C-G and A-T C-G: trip ...
Apple polygalacturonase inhibiting protein1 expressed in transgenic tobacco
... DQ185063), termed Mdpgip1, was identical to that isolated independently by Yao et al. (1999) from an apple fruit (cv. Golden delicious) cDNA library (Genbank accession no. U77041). The Mdpgip1 gene was engineered for high-level expression under control of the enhanced CaMV 35S promoter and terminato ...
... DQ185063), termed Mdpgip1, was identical to that isolated independently by Yao et al. (1999) from an apple fruit (cv. Golden delicious) cDNA library (Genbank accession no. U77041). The Mdpgip1 gene was engineered for high-level expression under control of the enhanced CaMV 35S promoter and terminato ...
This article appeared in a journal published by Elsevier. The
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... licensing copies, or posting to personal, institutional or third party websites are prohibited. In most cases authors are permitted to post their version of the article (e.g. in Word or Tex form) to their personal website or ...
Point mutation
A point mutation, or single base modification, is a type of mutation that causes a single nucleotide base change, insertion, or deletion of the genetic material, DNA or RNA. The term frameshift mutation indicates the addition or deletion of a base pair. A point mutant is an individual that is affected by a point mutation.Repeat induced point mutations are recurring point mutations, discussed below.