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Identification of a mitochondrial ATP synthase small subunit gene
Identification of a mitochondrial ATP synthase small subunit gene

... distributed throughout the mitochondria, as can be observed by RMtATP6-GFP/MitoTracker double staining (Fig. 3a–c). The RMtATP6-GFP gene was cloned into the plant expression vector pBI121 to study the localization of RMtATP6 in a tobacco plant (Nicotiana tabacum). The fusion gene was placed under th ...
Salmonella Typhi
Salmonella Typhi

... the cell and capable of replicating independently of it. Plasmids carry a signal situated at their replication origin dictating how many copies are to be made, and this number can be artificially increased. ...
DNA Technology and Genomics  I.
DNA Technology and Genomics I.

... Because the target sequence usually occurs (by chance) many times on a long DNA molecule, an enzyme will make many cuts. Copies of a DNA molecule will always yield the same set of restriction fragments when exposed to a specific enzyme. ...
mb_ch10
mb_ch10

... – During translation, amino acids are assembled from information encoded in mRNA. – As the mRNA codons move through the ribosome, tRNAs add specific amino acids to the growing polypeptide chain. – The process continues until a stop codon is reached and the newly made protein is released. ...
DNA chips: a new tool for genetic analysis and diagnostics
DNA chips: a new tool for genetic analysis and diagnostics

... mutated allele found corresponded to GGC (Gly) and GAC (Asp). The genotypes scored by the polypyrrole DNA chip assay were 100% in agreement with conventional DNA sequencing results. All the K-ras codon 12 mutations detected by direct sequencing were positive by polypyrrole DNA chip analysis, and no ...
Evolutionary history of the genus Capra
Evolutionary history of the genus Capra

... ideal for phylogenetic studies. It is haploid, with no recombination and uniquely maternal inheritance. Likewise, the Y-chromosome is a useful molecule for phylogenetic studies. This sex chromosome is paternally inherited and, with the exception of the pseudoautosomal region, it does not undergo hom ...
INTRODUCTION: - the BIOTECH Project
INTRODUCTION: - the BIOTECH Project

... comparing a broader range of organisms (i.e., spanning greater phylogenetic distances) than is whole genome DNA-DNA hybridization. However, as with DNA-DNA measurements, it was necessary to have DNA and/or RNA from each species of interest. If relationships were analyzed by comparing sequence data, ...
Chromosome - s3.amazonaws.com
Chromosome - s3.amazonaws.com

... Sequence of 3 bases on DNA or ‘triplet’ that codes for a particular amino acid. Gene (s) Genes A section of DNA that contains coded information as a sequence of bases. Genes code for polypeptides (small proteins). It is these polypeptides that determine the nature and development of organisms. Allel ...
KS4 Chromosomes, Genes and DNA
KS4 Chromosomes, Genes and DNA

... In all living things, characteristics are passed on in the chromosomes that offspring inherit from their parents. So all human characteristics, including gender, must be something to do with chromosomes. Where are chromosomes found? 4 of 47 ...
Document
Document

... • for fragments up to about 1,000 bases long • many identical copies of single, denatured sections of DNA • replication is started from the 5’ end, just as in PCR • a small concentration of bases in the solution of one type is altered so that the replication of that DNA strand stops when the replica ...
DNA Technology - De Anza College
DNA Technology - De Anza College

... • Recombinant DNA techniques are used • To clone cellulase genes in bacteria • To produce large quantities of cellulase To sell to textile manufacturers ...
APDC Unit IX CC DNA Bio
APDC Unit IX CC DNA Bio

... • Genetic Engineering: process of manipulating genes and genomes • Biotechnology: process of manipulating organisms or their components for the purpose of making useful products. • Recombinant DNA: DNA that has been artificially made, using DNA from different sources – eg. Human gene inserted into E ...
II. Types of Mutations
II. Types of Mutations

... C. Insertions and deletions of larger DNA fragments 1. Gain or loss of 100-1000’s (or more) of bases 2. Results in complete loss of gene function 3. Some deletions might wipe out entire gene cluster 4. Translocation: ...


... • Said Anastasia was raised by a farmer, and later she married a nephew of her adopted parents and had a daughter, Eugene’s ...
Foundations of Biology
Foundations of Biology

... their genetic information to the nucleus. The hydrogenosome, a structure involved in carbohydrate metabolism found in some Archezoans (Muller, 1992), is now thought to represent a mitochondria that has lost its genetic information completely and along with that loss, the ability to do the Krebs cycl ...
Chromatin Structure 1
Chromatin Structure 1

... Early in development, one X chromosome in each existing cell is randomly inactivated by condensation into a tight mass of heterochromatin. The inactivated X chromosome is strongly methylated and does not participate in transcription initiation. After X chromosome inactivation in embryonic cell - all ...
AP Biology
AP Biology

...  Different types of cells in an organism have the same DNA (pp. 406-410;FIGURES 21.5,21.6) Cells differ in structure and function not because they contain different genes, but because they express different portions of a common genome; they have genomic equivalence. Differentiated cells from mature ...
mutations
mutations

... (2) Therefore, a base substitution in DNA might not result in any amino acid change in a protein c) Missense mutation resulting in the replacement of an amino acid with a similar amino acid (1) A substitution of one hydrophobic amino acid for another hydrophobic amino acid might not disrupt the stru ...
DNA, RNA, and Protein Synthesis Note Packet
DNA, RNA, and Protein Synthesis Note Packet

... 2. Messenger RNA is the _____________________ between the DNA in the nucleus and the ribosomes in the cytoplasm. 3. The instructions are carried in the form of _____________. The first codon is called the __________________. This is the point at which mRNA will attach to the ribosome. This tells the ...
CHAPTER 14 LECTURE NOTES: RECOMBINANT DNA
CHAPTER 14 LECTURE NOTES: RECOMBINANT DNA

... In this example, digestion with Enzyme 1 shows that there are two restriction sites for this enzyme, but does not reveal whether the 3 kb segment is in the middle or on the end of the digested sequence, which is 17 kb long. Combined digestion by both enzyme 1 and enzyme 2 leaves the 6 and 8 kb segme ...
CLARK LAP Wednesday March 26 2014 STRAWBERRY DNA
CLARK LAP Wednesday March 26 2014 STRAWBERRY DNA

... through the cheesecloth and into the tall glass until there is very little liquid left in the funnel (only wet pulp remains). How does the filtered strawberry liquid look? • Pour the filtered strawberry liquid from the tall glass into the small glass jar so that the jar is one quarter full. • Measur ...
Hybrid
Hybrid

... and 0.8 in the second population, the probability of an individual being heterozygous in each population is simply 2pq = 0.32. If the two populations interbreed, then the expected frequency of heterozygotes among the F1 hybrids is 0.68, which is only 2.13 times the expected frequency of heterozygote ...
Document
Document

... Female Male affected Female affected ...
Genome Assembly and Annotation
Genome Assembly and Annotation

... Multiple records for same loci common, Single records for each molecule of records can contradict each other major organisms No limit to species included Data exchanged among INSDC members ...
5.6 Mutations
5.6 Mutations

... Usually occurs between two nonhomologous chromosomes. Result is a fusion protein with an altered function ...
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Mitochondrial DNA



Mitochondrial DNA (mtDNA or mDNA) is the DNA located in mitochondria, cellular organelles within eukaryotic cells that convert chemical energy from food into a form that cells can use, adenosine triphosphate (ATP). Mitochondrial DNA is only a small portion of the DNA in a eukaryotic cell; most of the DNA can be found in the cell nucleus and, in plants, in the chloroplast.In humans, mitochondrial DNA can be assessed as the smallest chromosome coding for 37 genes and containing approximately 16,600 base pairs. Human mitochondrial DNA was the first significant part of the human genome to be sequenced. In most species, including humans, mtDNA is inherited solely from the mother.The DNA sequence of mtDNA has been determined from a large number of organisms and individuals (including some organisms that are extinct), and the comparison of those DNA sequences represents a mainstay of phylogenetics, in that it allows biologists to elucidate the evolutionary relationships among species. It also permits an examination of the relatedness of populations, and so has become important in anthropology and field biology.
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