How do bacteria respond to their environment?
... How does E. coli sense starvation for nitrogen? Is there a protein that binds ALL amino acids? ...
... How does E. coli sense starvation for nitrogen? Is there a protein that binds ALL amino acids? ...
Isolation and Characterization of Plastidic Glucose-6-Phosphate Dehydrogenase
... Plant cells contain plastids, organelles dedicated to performing specific biochemical processes including photosynthesis, starch and oil biosynthesis. Fatty acid biosynthesis in oil seeds occurs in one type of plastid termed the leucoplast. Anabolic metabolism in leucoplasts includes the production ...
... Plant cells contain plastids, organelles dedicated to performing specific biochemical processes including photosynthesis, starch and oil biosynthesis. Fatty acid biosynthesis in oil seeds occurs in one type of plastid termed the leucoplast. Anabolic metabolism in leucoplasts includes the production ...
Effects of Enzyme Concentration, Temperature, pH
... products, processes such as enzymatic hydrolysis of the fish proteins to produce a more marketable and functional protein hydrolysate should be considered (Aspmo et al. 2005; Liceaga-Gesualdo & Li-Chan 1999). The functional properties of fish protein hydrolysates prepared may be improved through par ...
... products, processes such as enzymatic hydrolysis of the fish proteins to produce a more marketable and functional protein hydrolysate should be considered (Aspmo et al. 2005; Liceaga-Gesualdo & Li-Chan 1999). The functional properties of fish protein hydrolysates prepared may be improved through par ...
311-320 - CiteSeerX
... Thus the increased intensity observed with the rapeseed protein cannot be correlated with its tyrosine and tryptophan content. Similarly in the case of the mustard 12 S protein, the greater intensity of the 294 nm peak than that of the 287 nm peak cannot also be correlated with the tyrosine and tryp ...
... Thus the increased intensity observed with the rapeseed protein cannot be correlated with its tyrosine and tryptophan content. Similarly in the case of the mustard 12 S protein, the greater intensity of the 294 nm peak than that of the 287 nm peak cannot also be correlated with the tyrosine and tryp ...
Strong association between mRNA folding strength and protein
... It is plausible that the observed correlation is due to lesserunderstood features of gene expression. One plausibility is related to self-folding versus aggregation of mRNA molecules. If self-intramoleculer folding is strong enough it can prevent aggregation of mRNA molecules by hybridization (illus ...
... It is plausible that the observed correlation is due to lesserunderstood features of gene expression. One plausibility is related to self-folding versus aggregation of mRNA molecules. If self-intramoleculer folding is strong enough it can prevent aggregation of mRNA molecules by hybridization (illus ...
Properties and sequence of the coenzyme B12
... according to Kell et al. [11] with 100 mM glycerol as carbon source and C. freundii as described previously [6]. E. coli was routinely cultivated at 30³C in LB medium [12], which was supplemented with ampicillin (100 Wg ml31 ) when necessary. Recombinant E. coli strains used for expression of the ge ...
... according to Kell et al. [11] with 100 mM glycerol as carbon source and C. freundii as described previously [6]. E. coli was routinely cultivated at 30³C in LB medium [12], which was supplemented with ampicillin (100 Wg ml31 ) when necessary. Recombinant E. coli strains used for expression of the ge ...
IEX and RP Method Development for the Separation of
... Proteins interact with the stationary phase due to charge. Separation based on differences in degree of charge. Sample is injected in a mobile phase buffer with a low salt concentration – this binds proteins to the column. Proteins are typically eluted at constant pH with increasing salt gradients ( ...
... Proteins interact with the stationary phase due to charge. Separation based on differences in degree of charge. Sample is injected in a mobile phase buffer with a low salt concentration – this binds proteins to the column. Proteins are typically eluted at constant pH with increasing salt gradients ( ...
Optimality models in biology nanocourse @ Harvard
... -> Science proceeds by rejection of theories and replacement ...
... -> Science proceeds by rejection of theories and replacement ...
Transcriptome and metabolite profiling the infection cycle of
... investigated both pathogen and host at two time points during the initial symptomless interaction (4- and 10-dpi) and at the onset of first symptoms (the transitional phase) which occurred at 13-dpi in these experiments. This study confirmed many previously published fungal transcriptional responses ...
... investigated both pathogen and host at two time points during the initial symptomless interaction (4- and 10-dpi) and at the onset of first symptoms (the transitional phase) which occurred at 13-dpi in these experiments. This study confirmed many previously published fungal transcriptional responses ...
A novel EVI1 gene family, MEL1, lacking a PR domain (MEL1S) is
... domain (DBD), MEL1 (or MEL1S) was inserted into an EcoRI site of the pM GAL4 DBD cloning vector (Clontech, Palo Alto, CA), resulting in GAL4 DBD-MEL1 (or GAL4 DBD-MEL1S). The GAL4 binding site in the pG5-CAT vector (Clontech) was inserted into the region before the promoter of pGL3-promoter vector ( ...
... domain (DBD), MEL1 (or MEL1S) was inserted into an EcoRI site of the pM GAL4 DBD cloning vector (Clontech, Palo Alto, CA), resulting in GAL4 DBD-MEL1 (or GAL4 DBD-MEL1S). The GAL4 binding site in the pG5-CAT vector (Clontech) was inserted into the region before the promoter of pGL3-promoter vector ( ...
Defining the anabolic window of opportunity
... muscle. The reasons for this lack of certainty likely are multi-factorial and may have to do with the differences in study design between these studies. In the studies described above, the subjects varied from completely untrained (20) to recreationally trained (12) to very welltrained (19). The imp ...
... muscle. The reasons for this lack of certainty likely are multi-factorial and may have to do with the differences in study design between these studies. In the studies described above, the subjects varied from completely untrained (20) to recreationally trained (12) to very welltrained (19). The imp ...
Biochemical and functional characterization of Plasmodium
... Background: Emergence of drug-resistant Plasmodium falciparum has created an urgent need for new drug targets. DNA polymerase δ is an essential enzyme required for chromosomal DNA replication and repair, and therefore may be a potential target for anti-malarial drug development. However, little is ...
... Background: Emergence of drug-resistant Plasmodium falciparum has created an urgent need for new drug targets. DNA polymerase δ is an essential enzyme required for chromosomal DNA replication and repair, and therefore may be a potential target for anti-malarial drug development. However, little is ...
Structural adaptation of enzymes to low
... at temperatures below 10°C. Indeed, the earth’s surface is dominated by low-temperature environments such as the Arctic and Antarctic continents, mountains regions and the marine waters which cover 70% of its area and display, below 1000 m under the sea level, temperatures not exceeding 5°C. Psychro ...
... at temperatures below 10°C. Indeed, the earth’s surface is dominated by low-temperature environments such as the Arctic and Antarctic continents, mountains regions and the marine waters which cover 70% of its area and display, below 1000 m under the sea level, temperatures not exceeding 5°C. Psychro ...
Journal of Bacteriology
... isolated as described previously (34). The primer was labeled with [g-32P]dATP (Amersham), using polynucleotide kinase (Pharmacia) as described by Maniatis et al. (22). Primer extension was performed by using avian myeloblastosis virus reverse transcriptase (Pharmacia) annealed on approximately 20 m ...
... isolated as described previously (34). The primer was labeled with [g-32P]dATP (Amersham), using polynucleotide kinase (Pharmacia) as described by Maniatis et al. (22). Primer extension was performed by using avian myeloblastosis virus reverse transcriptase (Pharmacia) annealed on approximately 20 m ...
Possibilities and Limitations of Genetic Engineering
... the species of interest can by hybridized. The gene can come from another plant species, or from a non-plant species, such as a bacterium. In alfalfa, the most commonly used method of gene transfer is Agrobacterium tumefaciens mediated gene transfer. A. tumefaciens is a naturally occurring soil bact ...
... the species of interest can by hybridized. The gene can come from another plant species, or from a non-plant species, such as a bacterium. In alfalfa, the most commonly used method of gene transfer is Agrobacterium tumefaciens mediated gene transfer. A. tumefaciens is a naturally occurring soil bact ...
5-Cell and Molecular Biology (Golgi etc)
... All are hydrolyses and for optimal activity they require an acid environment and Lysosome provides this by maintaining pH of about 5 in its interior In this way, the content of cytosol are doubly protected against attack by the cell’s own digestive system The membrane of lysosome normally ke ...
... All are hydrolyses and for optimal activity they require an acid environment and Lysosome provides this by maintaining pH of about 5 in its interior In this way, the content of cytosol are doubly protected against attack by the cell’s own digestive system The membrane of lysosome normally ke ...
Regional Differences in Protein Synthesis within the Lens of
... Utilization of 3SS-methionine by cells of the rat lens was studied by combined autoradiographic and chemical techniques. Part of the methionine teas converted to cysteine, glutathione, and taurine. Protein synthesis was essentially restricted to the lens cortex, and appeared to be largely attributab ...
... Utilization of 3SS-methionine by cells of the rat lens was studied by combined autoradiographic and chemical techniques. Part of the methionine teas converted to cysteine, glutathione, and taurine. Protein synthesis was essentially restricted to the lens cortex, and appeared to be largely attributab ...
Eh Klp5 is a divergent member of the kinesin 5 family that regulates
... monastrol. Mammalian cells treated with this drug arrest in mitosis with monopolar microtubular fibres due to specific inhibition of Eg5 activity (Mayer et al., 1999). Subsequently other Eg5 inhibitors – HR22C16 analogues were identified through chemical genetic screens (Hotha et al., 2003) that wer ...
... monastrol. Mammalian cells treated with this drug arrest in mitosis with monopolar microtubular fibres due to specific inhibition of Eg5 activity (Mayer et al., 1999). Subsequently other Eg5 inhibitors – HR22C16 analogues were identified through chemical genetic screens (Hotha et al., 2003) that wer ...
intolerance to lactose and other dietary sugars
... high frequency of the lactase persistence allele in certain populations probably results from selection for milk drinking, due the nutritional value of milk. We have shown that the lactase persistence/nonpersistence polymorphism is controlled by a cis-acting regulatory element (Fig. 2) and have prov ...
... high frequency of the lactase persistence allele in certain populations probably results from selection for milk drinking, due the nutritional value of milk. We have shown that the lactase persistence/nonpersistence polymorphism is controlled by a cis-acting regulatory element (Fig. 2) and have prov ...
Molecular Imprinting of Maltose Binding Protein
... mixed in a volume of 10 mM Tris buffer (pH 7) to obtain a total monomer concentration of 10 wt %. To initiate polymerization, 5 μL of TEMED (6.5% v/v, aqueous solution) was added to 50 μL of precursor solution and then immediately deposited on a silane-modified glass slide and covered by a mica wafer. ...
... mixed in a volume of 10 mM Tris buffer (pH 7) to obtain a total monomer concentration of 10 wt %. To initiate polymerization, 5 μL of TEMED (6.5% v/v, aqueous solution) was added to 50 μL of precursor solution and then immediately deposited on a silane-modified glass slide and covered by a mica wafer. ...
Structural bases of GM1 gangliosidosis and Morquio B
... the enzyme molecule. The numbers of atoms affected by the aminoacid substitutions in the main chain and side chain are 397 and 495, respectively, and the RMSD value is 0.17 Å (Table 1). This suggests that G123R causes a large conformational change. Color imaging clearly showed a large conformationa ...
... the enzyme molecule. The numbers of atoms affected by the aminoacid substitutions in the main chain and side chain are 397 and 495, respectively, and the RMSD value is 0.17 Å (Table 1). This suggests that G123R causes a large conformational change. Color imaging clearly showed a large conformationa ...
Plant biotin-containing carboxylases
... functional domains (Fig. 1) [3,4]. These domains can be readily recognized based upon a high degree of sequence conservation. Two are catalytic, and the third is a structural domain. The structural domain that carries the biotin prosthetic group is called the biotin carboxylcarrier (BCC)1 domain. On ...
... functional domains (Fig. 1) [3,4]. These domains can be readily recognized based upon a high degree of sequence conservation. Two are catalytic, and the third is a structural domain. The structural domain that carries the biotin prosthetic group is called the biotin carboxylcarrier (BCC)1 domain. On ...
protein
... • Peptide is the name assigned to short polymers of amino acids. Peptides are classified by the number of amino acid units in the chain. • Each unit is called an amino acid residue, the word residue denoting what is left after the release of H2O when an amino acid forms a peptide link upon joining t ...
... • Peptide is the name assigned to short polymers of amino acids. Peptides are classified by the number of amino acid units in the chain. • Each unit is called an amino acid residue, the word residue denoting what is left after the release of H2O when an amino acid forms a peptide link upon joining t ...
Reducing Rice Seed Storage Protein Accumulation Leads to
... visible on a stained gel (Fig. 1A and Supplemental Fig. S1). On the other hand, levels of Glb-1 and 13kD prolamins significantly increased (Fig. 1A). GluB-less seeds had decreased levels of GluB-4, GluB-2 and GluB-1, whereas levels of Glb-1 and 13kD prolamins increased appreciably (Fig. 1A and Supp ...
... visible on a stained gel (Fig. 1A and Supplemental Fig. S1). On the other hand, levels of Glb-1 and 13kD prolamins significantly increased (Fig. 1A). GluB-less seeds had decreased levels of GluB-4, GluB-2 and GluB-1, whereas levels of Glb-1 and 13kD prolamins increased appreciably (Fig. 1A and Supp ...
- Wiley Online Library
... that B. subtilis has two distinct nitrate reductases, one is assimilatory and the other respiratory [1,3,13]. In retrospect, the nitrate reductase reported above should be the respiratory one encoded by narGHJI since the enzyme was shown later to be induced under anaerobiosis and not under the contr ...
... that B. subtilis has two distinct nitrate reductases, one is assimilatory and the other respiratory [1,3,13]. In retrospect, the nitrate reductase reported above should be the respiratory one encoded by narGHJI since the enzyme was shown later to be induced under anaerobiosis and not under the contr ...